This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baldridge, J. R.
Right arrow Articles by Buchmeier, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baldridge, J. R.
Right arrow Articles by Buchmeier, M. J.

 Previous Article  |  Next Article 

J. Virol., 01 1997, 755-758, Vol 71, No. 1
Copyright © 1997, American Society for Microbiology

Antibody prevents the establishment of persistent arenavirus infection in synergy with endogenous T cells

JR Baldridge, TS McGraw, A Paoletti and MJ Buchmeier
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.

A cardinal feature of the biology of lymphocytic choriomeningitis virus (LCMV) is its ability to establish persistent infections in mice. Persistence is usually established by infection of the mouse during the in utero or neonatal period. Susceptibility can be extended to the adult by treatment with immunosuppressive agents or by infection with immunosuppressive strains of LCMV. In this study we investigated the capacity of passively acquired anti-LCMV antibodies to prevent the establishment of persistence in both neonatal and adult mice. Suckling BALB/c mouse pups nursed by mothers immunized against LCMV before pregnancy had higher survival rates following infection than controls and withstood challenge doses of up to 400 PFU without becoming persistently infected. To establish that maternal antibody alone and not maternally derived T cells provided this protection, nonimmune mothers were infused with monoclonal anti-LCMV neutralizing antibodies within 24 h after delivering their pups. Pups nursing on these passively immunized mothers were resistant to persistent LCMV infection. The establishment of persistence in adult BALB/c mice by the immunosuppressive, macrophage-tropic LCMV variant, clone 13 was also prevented by prophylactic treatment with anti-LCMV monoclonal antibodies. However, the protection afforded by passively acquired antibody was found to be incomplete if the recipients lacked functional CD8+ T cells. While 65% of neonatal athymic (nu/nu) mice nursed by immune nu/+ dams resisted low-dose viral challenge (25 PFU), the majority of nude pups challenged with high doses of virus (100 PFU) became persistently infected. Also, protection was incomplete in beta2- microglobulin knockout mice, which lack functional CD8+ T cells, suggesting that a cooperative effect was exerted by the combination of neutralizing antibody and endogenous T cells. These results indicate that antibodies provide an effective barrier to the establishment of persistent infections in immunocompetent mice and reaffirm that vaccines which induce strong humoral responses may provide efficient protection against arenavirus infections.


This article has been cited by other articles:

  • Crotty, S., McCausland, M. M., Aubert, R. D., Wherry, E. J., Ahmed, R. (2006). Hypogammaglobulinemia and exacerbated CD8 T-cell-mediated immunopathology in SAP-deficient mice with chronic LCMV infection mimics human XLP disease. Blood 108: 3085-3093 [Abstract] [Full Text]  
  • Botten, J., Alexander, J., Pasquetto, V., Sidney, J., Barrowman, P., Ting, J., Peters, B., Southwood, S., Stewart, B., Rodriguez-Carreno, M. P., Mothe, B., Whitton, J. L., Sette, A., Buchmeier, M. J. (2006). Identification of Protective Lassa Virus Epitopes That Are Restricted by HLA-A2.. J. Virol. 80: 8351-8361 [Abstract] [Full Text]  
  • Gros, L., Dreja, H., Fiser, A. L., Plays, M., Pelegrin, M., Piechaczyk, M. (2005). Induction of Long-Term Protective Antiviral Endogenous Immune Response by Short Neutralizing Monoclonal Antibody Treatment. J. Virol. 79: 6272-6280 [Abstract] [Full Text]  
  • Casadevall, A., Pirofski, L.-a. (2004). New Concepts in Antibody-Mediated Immunity. Infect. Immun. 72: 6191-6196 [Full Text]  
  • Jordan, M. B., Hildeman, D., Kappler, J., Marrack, P. (2004). An animal model of hemophagocytic lymphohistiocytosis (HLH): CD8+ T cells and interferon gamma are essential for the disorder. Blood 104: 735-743 [Abstract] [Full Text]  
  • Krieg, C., Maier, R., Meyerhans, A. (2004). Gut-homing ({alpha}4{beta}7+) Th1 memory responses after inactivated poliovirus immunization in poliovirus orally pre-immunized donors. J. Gen. Virol. 85: 1571-1579 [Abstract] [Full Text]  
  • Schuurhuis, D. H., Ioan-Facsinay, A., Nagelkerken, B., van Schip, J. J., Sedlik, C., Melief, C. J. M., Verbeek, J. S., Ossendorp, F. (2002). Antigen-Antibody Immune Complexes Empower Dendritic Cells to Efficiently Prime Specific CD8+ CTL Responses In Vivo. J. Immunol. 168: 2240-2246 [Abstract] [Full Text]  
  • Ou, R., Zhou, S., Huang, L., Moskophidis, D. (2001). Critical Role for Alpha/Beta and Gamma Interferons in Persistence of Lymphocytic Choriomeningitis Virus by Clonal Exhaustion of Cytotoxic T Cells. J. Virol. 75: 8407-8423 [Abstract] [Full Text]  
  • Ciurea, A., Hunziker, L., Klenerman, P., Hengartner, H., Zinkernagel, R. M. (2001). Impairment of Cd4+ T Cell Responses during Chronic Virus Infection Prevents Neutralizing Antibody Responses against Virus Escape Mutants. JEM 193: 297-306 [Abstract] [Full Text]  
  • Earl, P. L., Sugiura, W., Montefiori, D. C., Broder, C. C., Lee, S. A., Wild, C., Lifson, J., Moss, B. (2001). Immunogenicity and Protective Efficacy of Oligomeric Human Immunodeficiency Virus Type 1 gp140. J. Virol. 75: 645-653 [Abstract] [Full Text]  
  • Fisher-Hoch, S. P., Hutwagner, L., Brown, B., McCormick, J. B. (2000). Effective Vaccine for Lassa Fever. J. Virol. 74: 6777-6783 [Abstract] [Full Text]  
  • Seiler, P., Senn, B. M., Klenerman, P., Kalinke, U., Hengartner, H., Zinkernagel, R. M. (2000). Additive Effect of Neutralizing Antibody and Antiviral Drug Treatment in Preventing Virus Escape and Persistence. J. Virol. 74: 5896-5901 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»