This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Usherwood, E. J.
Right arrow Articles by Nash, A. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Usherwood, E. J.
Right arrow Articles by Nash, A. A.

 Previous Article  |  Next Article 

J. Virol., Sep 1996, 6516-6518, Vol 70, No. 9
Copyright © 1996, American Society for Microbiology

Characterization of tumor cell lines derived from murine gammaherpesvirus-68-infected mice

EJ Usherwood, JP Stewart and AA Nash
Department of Veterinary Pathology, University of Edinburgh, Summerhall, United Kingdom. Edward.Usherwood@ed.ac.uk

Cell lines were derived from mice with murine gammaherpesvirus-68 (MHV- 68)-associated lymphoproliferative disease. Four were of an ambiguous phenotype and were MHV-68 negative. One, S11, was a B lymphocyte that contained MHV-68 genomes in both linear and episomal forms and released virus. The line was clonable and grew into tumors in nude mice. This is the first naturally occurring MHV-68-positive B-cell line to be generated, and it will be an invaluable tool for the study of MHV-68 latency.


This article has been cited by other articles:

  • Flach, B., Steer, B., Thakur, N. N., Haas, J., Adler, H. (2009). The M10 Locus of Murine Gammaherpesvirus 68 Contributes to both the Lytic and the Latent Phases of Infection. J. Virol. 83: 8163-8172 [Abstract] [Full Text]  
  • Krug, L. T., Collins, C. M., Gargano, L. M., Speck, S. H. (2009). NF-{kappa}B p50 Plays Distinct Roles in the Establishment and Control of Murine Gammaherpesvirus 68 Latency. J. Virol. 83: 4732-4748 [Abstract] [Full Text]  
  • Gargano, L. M., Forrest, J. C., Speck, S. H. (2009). Signaling through Toll-Like Receptors Induces Murine Gammaherpesvirus 68 Reactivation In Vivo. J. Virol. 83: 1474-1482 [Abstract] [Full Text]  
  • Jarousse, N., Chandran, B., Coscoy, L. (2008). Lack of Heparan Sulfate Expression in B-Cell Lines: Implications for Kaposi's Sarcoma-Associated Herpesvirus and Murine Gammaherpesvirus 68 Infections. J. Virol. 82: 12591-12597 [Abstract] [Full Text]  
  • Forrest, J. C., Speck, S. H. (2008). Establishment of B-Cell Lines Latently Infected with Reactivation-Competent Murine Gammaherpesvirus 68 Provides Evidence for Viral Alteration of a DNA Damage-Signaling Cascade. J. Virol. 82: 7688-7699 [Abstract] [Full Text]  
  • Hochreiter, R., Ptaschinski, C., Kunkel, S. L., Rochford, R. (2007). Murine gammaherpesvirus-68 productively infects immature dendritic cells and blocks maturation. J. Gen. Virol. 88: 1896-1905 [Abstract] [Full Text]  
  • Donofrio, G., Herath, S., Sartori, C., Cavirani, S., Flammini, C. F., Sheldon, I. M. (2007). Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function. Reproduction 134: 183-197 [Abstract] [Full Text]  
  • Donofrio, G., Cavirani, S., Vanderplasschen, A., Gillet, L., Flammini, C. F. (2006). Recombinant Bovine Herpesvirus 4 (BoHV-4) Expressing Glycoprotein D of BoHV-1 Is Immunogenic and Elicits Serum-Neutralizing Antibodies against BoHV-1 in a Rabbit Model. CVI 13: 1246-1254 [Abstract] [Full Text]  
  • Rodrigues, L., Pires de Miranda, M., Caloca, M. J., Bustelo, X. R., Simas, J. P. (2006). Activation of Vav by the Gammaherpesvirus M2 Protein Contributes to the Establishment of Viral Latency in B Lymphocytes.. J. Virol. 80: 6123-6135 [Abstract] [Full Text]  
  • Moser, J. M., Upton, J. W., Gray, K. S., Speck, S. H. (2005). Ex Vivo Stimulation of B Cells Latently Infected with Gammaherpesvirus 68 Triggers Reactivation from Latency. J. Virol. 79: 5227-5231 [Abstract] [Full Text]  
  • Flano, E., Jia, Q., Moore, J., Woodland, D. L., Sun, R., Blackman, M. A. (2005). Early Establishment of {gamma}-Herpesvirus Latency: Implications for Immune Control. J. Immunol. 174: 4972-4978 [Abstract] [Full Text]  
  • Rickabaugh, T. M., Brown, H. J., Wu, T.-T., Song, M. J., Hwang, S., Deng, H., Mitsouras, K., Sun, R. (2005). Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 RTA Reactivates Murine Gammaherpesvirus 68 from Latency. J. Virol. 79: 3217-3222 [Abstract] [Full Text]  
  • Deng, H., Chu, J. T., Park, N.-H., Sun, R. (2004). Identification of cis Sequences Required for Lytic DNA Replication and Packaging of Murine Gammaherpesvirus 68. J. Virol. 78: 9123-9131 [Abstract] [Full Text]  
  • Moorman, N. J., Willer, D. O., Speck, S. H. (2003). The Gammaherpesvirus 68 Latency-Associated Nuclear Antigen Homolog Is Critical for the Establishment of Splenic Latency. J. Virol. 77: 10295-10303 [Abstract] [Full Text]  
  • Martinez-Guzman, D., Rickabaugh, T., Wu, T.-T., Brown, H., Cole, S., Song, M. J., Tong, L., Sun, R. (2003). Transcription Program of Murine Gammaherpesvirus 68. J. Virol. 77: 10488-10503 [Abstract] [Full Text]  
  • Macrae, A. I., Usherwood, E. J., Husain, S. M., Flano, E., Kim, I.-J., Woodland, D. L., Nash, A. A., Blackman, M. A., Sample, J. T., Stewart, J. P. (2003). Murid Herpesvirus 4 Strain 68 M2 Protein Is a B-Cell-Associated Antigen Important for Latency but Not Lymphocytosis. J. Virol. 77: 9700-9709 [Abstract] [Full Text]  
  • Marques, S., Efstathiou, S., Smith, K. G., Haury, M., Simas, J. P. (2003). Selective Gene Expression of Latent Murine Gammaherpesvirus 68 in B Lymphocytes. J. Virol. 77: 7308-7318 [Abstract] [Full Text]  
  • Pavlova, I. V., Virgin, H. W. IV, Speck, S. H. (2003). Disruption of Gammaherpesvirus 68 Gene 50 Demonstrates that Rta Is Essential for Virus Replication. J. Virol. 77: 5731-5739 [Abstract] [Full Text]  
  • Rosbottom, J., Dalziel, R. G., Reid, H. W., Stewart, J. P. (2002). Ovine herpesvirus 2 lytic cycle replication and capsid production. J. Gen. Virol. 83: 2999-3002 [Abstract] [Full Text]  
  • Flano, E., Kim, I.-J., Woodland, D. L., Blackman, M. A. (2002). {gamma}-Herpesvirus Latency Is Preferentially Maintained in Splenic Germinal Center and Memory B Cells. JEM 196: 1363-1372 [Abstract] [Full Text]  
  • Jacoby, M. A., Virgin, H. W. IV, Speck, S. H. (2002). Disruption of the M2 Gene of Murine Gammaherpesvirus 68 Alters Splenic Latency following Intranasal, but Not Intraperitoneal, Inoculation. J. Virol. 76: 1790-1801 [Abstract] [Full Text]  
  • Usherwood, E. J., Ward, K. A., Blackman, M. A., Stewart, J. P., Woodland, D. L. (2001). Latent Antigen Vaccination in a Model Gammaherpesvirus Infection. J. Virol. 75: 8283-8288 [Abstract] [Full Text]  
  • Rochford, R., Lutzke, M. L., Alfinito, R. S., Clavo, A., Cardin, R. D. (2001). Kinetics of Murine Gammaherpesvirus 68 Gene Expression following Infection of Murine Cells in Culture and in Mice. J. Virol. 75: 4955-4963 [Abstract] [Full Text]  
  • Donofrio, G., van Santen, V. L. (2001). A bovine macrophage cell line supports bovine herpesvirus-4 persistent infection. J. Gen. Virol. 82: 1181-1185 [Abstract] [Full Text]  
  • Robertson, K. A., Usherwood, E. J., Nash, A. A. (2001). Regression of a Murine Gammaherpesvirus 68-Positive B-Cell Lymphoma Mediated by CD4 T Lymphocytes. J. Virol. 75: 3480-3482 [Abstract] [Full Text]  
  • Ensser, A., Glykofrydes, D., Niphuis, H., Kuhn, E. M., Rosenwirth, B., Heeney, J. L., Niedobitek, G., Muller-Fleckenstein, I., Fleckenstein, B. (2001). Independence of Herpesvirus-Induced T Cell Lymphoma from Viral Cyclin D Homologue. JEM 193: 637-642 [Abstract] [Full Text]  
  • Terry, L. A., Stewart, J. P., Nash, A. A., Fazakerley, J. K. (2000). Murine gammaherpesvirus-68 infection of and persistence in the central nervous system. J. Gen. Virol. 81: 2635-2643 [Abstract] [Full Text]  
  • Usherwood, E. J., Roy, D. J., Ward, K., Surman, S. L., Dutia, B. M., Blackman, M. A., Stewart, J. P., Woodland, D. L. (2000). Control of Gammaherpesvirus Latency by Latent Antigen-Specific Cd8+ T Cells. JEM 192: 943-952 [Abstract] [Full Text]  
  • Donofrio, G., Cavirani, S., van Santen, V. L. (2000). Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus. J. Gen. Virol. 81: 1807-1814 [Abstract] [Full Text]  
  • Wu, T.-T., Usherwood, E. J., Stewart, J. P., Nash, A. A., Sun, R. (2000). Rta of Murine Gammaherpesvirus 68 Reactivates the Complete Lytic Cycle from Latency. J. Virol. 74: 3659-3667 [Abstract] [Full Text]  
  • Dutia, B. M., Stewart, J. P., Clayton, R. A. E., Dyson, H., Nash, A. A. (1999). Kinetic and phenotypic changes in murine lymphocytes infected with murine gammaherpesvirus-68 in vitro. J. Gen. Virol. 80: 2729-2736 [Abstract] [Full Text]  
  • Husain, S. M., Usherwood, E. J., Dyson, H., Coleclough, C., Coppola, M. A., Woodland, D. L., Blackman, M. A., Stewart, J. P., Sample, J. T. (1999). Murine gammaherpesvirus M2 gene is latency-associated and its protein a target for CD8+ T lymphocytes. Proc. Natl. Acad. Sci. USA 96: 7508-7513 [Abstract] [Full Text]  
  • Virgin, H. W. IV, Presti, R. M., Li, X.-Y., Liu, C., Speck, S. H. (1999). Three Distinct Regions of the Murine Gammaherpesvirus 68 Genome Are Transcriptionally Active in Latently Infected Mice. J. Virol. 73: 2321-2332 [Abstract] [Full Text]  
  • Simas, J., Swann, D, Bowden, R, Efstathiou, S (1999). Analysis of murine gammaherpesvirus-68 transcription during lytic and latent infection. J. Gen. Virol. 80: 75-82 [Abstract]  
  • Stewart, J. P., Usherwood, E. J., Ross, A., Dyson, H., Nash, T. (1998). Lung Epithelial Cells Are a Major Site of Murine Gammaherpesvirus Persistence. JEM 187: 1941-1951 [Abstract] [Full Text]  
  • Neyts, J., De Clercq, E. (1998). In Vitro and In Vivo Inhibition of Murine Gamma Herpesvirus 68 Replication by Selected Antiviral Agents. Antimicrob. Agents Chemother. 42: 170-172 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»