This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palmarini, M.
Right arrow Articles by Sharp, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palmarini, M.
Right arrow Articles by Sharp, J. M.

 Previous Article  |  Next Article 

J. Virol., 03 1996, 1618-1623, Vol 70, No. 3
Copyright © 1996, American Society for Microbiology

The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep

M Palmarini, C Cousens, RG Dalziel, J Bai, K Stedman, JC DeMartini and JM Sharp
Moredun Research Institute, Edinburgh, United Kingdom.

Sheep pulmonary adenomatosis ([SPA] ovine pulmonary carcinoma) is a transmissible lung cancer of sheep that has been associated etiologically with a type D- and B-related retrovirus (jaagsiekte retrovirus (JSRV]). To date it has been impossible to cultivate JSRV in vitro and therefore to demonstrate the etiology of SPA by a classical approach. In addition, the presence of 15 to 20 copies of endogenous JSRV-related sequences (enJSRV) has hampered studies at the molecular level. The aim of this study was to investigate whether the expression of exogenous JSRV was specifically associated with neoplasia in SPA- affected animals. Initially, we found that enJSRVs were transcribed in a wide variety of normal sheep tissues. Then, by sequencing part of the gag gene of enJSRV we established a ScaI restriction site in gag as a molecular marker for the exogenous form of JSRV. Restriction enzyme digestion of PCR products obtained from the amplification of cDNA from a total of 65 tissues collected from SPA-affected and unaffected control sheep revealed that the exogenous form of JSRV was exclusively and consistently present in tumor tissues and lung secretions of the affected animals. In addition, exogenous JSRV provirus was detected only in DNA from SPA tumors and not from nontumor tissues of the same animals. This study has demonstrated clearly that the exogenous form of JSRV is specifically associated with SPA tumors.


This article has been cited by other articles:

  • Angeloni, D. (2007). Molecular analysis of deletions in human chromosome 3p21 and the role of resident cancer genes in disease. Brief Funct Genomic Proteomic 0: elm007v1-21 [Abstract] [Full Text]  
  • Caporale, M., Cousens, C., Centorame, P., Pinoni, C., De las Heras, M., Palmarini, M. (2006). Expression of the jaagsiekte sheep retrovirus envelope glycoprotein is sufficient to induce lung tumors in sheep.. J. Virol. 80: 8030-8037 [Abstract] [Full Text]  
  • McGee-Estrada, K., Fan, H. (2006). In Vivo and In Vitro Analysis of Factor Binding Sites in Jaagsiekte Sheep Retrovirus Long Terminal Repeat Enhancer Sequences: Roles of HNF-3, NF-I, and C/EBP for Activity in Lung Epithelial Cells. J. Virol. 80: 332-341 [Abstract] [Full Text]  
  • Palmarini, M., Mura, M., Spencer, T. E. (2004). Endogenous betaretroviruses of sheep: teaching new lessons in retroviral interference and adaptation. J. Gen. Virol. 85: 1-13 [Abstract] [Full Text]  
  • Carlson, J., Lyon, M., Bishop, J., Vaiman, A., Cribiu, E., Mornex, J.-F., Brown, S., Knudson, D., DeMartini, J., Leroux, C. (2003). Chromosomal Distribution of Endogenous Jaagsiekte Sheep Retrovirus Proviral Sequences in the Sheep Genome. J. Virol. 77: 9662-9668 [Abstract] [Full Text]  
  • Grossman, D. A., Hiti, A. L., McNiel, E. A., Ye, Y., Alpaugh, M. L., Barsky, S. H. (2002). Comparative Oncological Studies of Feline Bronchioloalveolar Lung Carcinoma, Its Derived Cell Line and Xenograft. Cancer Res. 62: 3826-3833 [Abstract] [Full Text]  
  • Summers, C., Neill, W., Dewar, P., Gonzalez, L., van der Molen, R., Norval, M., Sharp, J. M. (2002). Systemic immune responses following infection with Jaagsiekte sheep retrovirus and in the terminal stages of ovine pulmonary adenocarcinoma. J. Gen. Virol. 83: 1753-1757 [Abstract] [Full Text]  
  • Palmarini, M., Gray, C. A., Carpenter, K., Fan, H., Bazer, F. W., Spencer, T. E. (2001). Expression of Endogenous Betaretroviruses in the Ovine Uterus: Effects of Neonatal Age, Estrous Cycle, Pregnancy, and Progesterone. J. Virol. 75: 11319-11327 [Abstract] [Full Text]  
  • Palmarini, M., Maeda, N., Murgia, C., De-Fraja, C., Hofacre, A., Fan, H. (2001). A Phosphatidylinositol 3-Kinase Docking Site in the Cytoplasmic Tail of the Jaagsiekte Sheep Retrovirus Transmembrane Protein Is Essential for Envelope-Induced Transformation of NIH 3T3 Cells. J. Virol. 75: 11002-11009 [Abstract] [Full Text]  
  • Palmarini, M., Fan, H. (2001). Retrovirus-Induced Ovine Pulmonary Adenocarcinoma, an Animal Model for Lung Cancer. JNCI J Natl Cancer Inst 93: 1603-1614 [Abstract] [Full Text]  
  • Maeda, N., Palmarini, M., Murgia, C., Fan, H. (2001). Direct transformation of rodent fibroblasts by jaagsiekte sheep retrovirus DNA. Proc. Natl. Acad. Sci. USA 98: 4449-4454 [Abstract] [Full Text]  
  • Palmarini, M., Hallwirth, C., York, D., Murgia, C., de Oliveira, T., Spencer, T., Fan, H. (2000). Molecular Cloning and Functional Analysis of Three Type D Endogenous Retroviruses of Sheep Reveal a Different Cell Tropism from That of the Highly Related Exogenous Jaagsiekte Sheep Retrovirus. J. Virol. 74: 8065-8076 [Abstract] [Full Text]  
  • Palmarini, M., Datta, S., Omid, R., Murgia, C., Fan, H. (2000). The Long Terminal Repeat of Jaagsiekte Sheep Retrovirus Is Preferentially Active in Differentiated Epithelial Cells of the Lungs. J. Virol. 74: 5776-5787 [Abstract] [Full Text]  
  • Rai, S. K., DeMartini, J. C., Miller, A. D. (2000). Retrovirus Vectors Bearing Jaagsiekte Sheep Retrovirus Env Transduce Human Cells by Using a New Receptor Localized to Chromosome 3p21.3. J. Virol. 74: 4698-4704 [Abstract] [Full Text]  
  • Palmarini, M., Sharp, J. M., Lee, C., Fan, H. (1999). In Vitro Infection of Ovine Cell Lines by Jaagsiekte Sheep Retrovirus. J. Virol. 73: 10070-10078 [Abstract] [Full Text]  
  • Palmarini, M., Sharp, J. M., de las Heras, M., Fan, H. (1999). Jaagsiekte Sheep Retrovirus Is Necessary and Sufficient To Induce a Contagious Lung Cancer in Sheep. J. Virol. 73: 6964-6972 [Abstract] [Full Text]  
  • Cousens, C., Minguijon, E., Dalziel, R. G., Ortin, A., Garcia, M., Park, J., Gonzalez, L., Sharp, J. M., de las Heras, M. (1999). Complete Sequence of Enzootic Nasal Tumor Virus, a Retrovirus Associated with Transmissible Intranasal Tumors of Sheep. J. Virol. 73: 3986-3993 [Abstract] [Full Text]  
  • Holland, M. J., Palmarini, M., Garcia-Goti, M., Gonzalez, L., McKendrick, I., de las Heras, M., Sharp, J. M. (1999). Jaagsiekte Retrovirus Is Widely Distributed both in T and B Lymphocytes and in Mononuclear Phagocytes of Sheep with Naturally and Experimentally Acquired Pulmonary Adenomatosis. J. Virol. 73: 4004-4008 [Abstract] [Full Text]  
  • Blond, J.-L., Besème, F., Duret, L., Bouton, O., Bedin, F., Perron, H., Mandrand, B., Mallet, F. (1999). Molecular Characterization and Placental Expression of HERV-W, a New Human Endogenous Retrovirus Family. J. Virol. 73: 1175-1185 [Abstract] [Full Text]  
  • Perron, H., Garson, J. A., Bedin, F., Beseme, F., Paranhos-Baccala, G., Komurian-Pradel, F., Mallet, F., Tuke, P. W., Voisset, C., Blond, J. L., Lalande, B., Seigneurin, J. M., Mandrand, B., Sclerosis, t. C. R. G. o. M. (1997). Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis. Proc. Natl. Acad. Sci. USA 94: 7583-7588 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»