This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sock, E.
Right arrow Articles by Wegner, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sock, E.
Right arrow Articles by Wegner, M.

 Previous Article  |  Next Article 

J. Virol., Mar 1996, 1512-1520, Vol 70, No. 3
Copyright © 1996, American Society for Microbiology

Functional comparison of PML-type and archetype strains of JC virus

E Sock, K Renner, D Feist, H Leger and M Wegner
Zentrum fur Molekulare Neurobiologie, Universitat Hamburg, Germany.

Isolates of the human polyomavirus JC can be grouped as either PML-type or archetype strains primarily on the basis of divergence in their regulatory regions. Only PML-type viruses have so far been found to be associated with the human demyelinating disease progressive multifocal leukoencephalopathy. Here we have compared the functional properties of archetype and PML-type regulatory regions with regard to DNA replication and viral gene expression. No significant differences could be detected between archetype and PML-type regions in their ability to direct episomal DNA replication in the presence of JC virus T antigen. When viral gene expression was examined, early- and late-gene promoters from all PML-type strains exhibited a significantly higher activity in glial than in nonglial cells. Surprisingly, archetype strain promoters were also preferentially active in glial cells, although this effect was less pronounced than in PML-type strains. Furthermore, all promoters from archetype strains reacted to the presence of viral T antigen or the glial transcription factor Tst-1/Oct6 in a manner similar to the promoters of the PML-type viral strain Mad-1. Interestingly, T antigen and Tst-1/Oct6 were found to function in a species-specific and cell-type-specific manner, respectively. We concluded from our experiments that the differences in the regulatory regions cannot account for the different biology of archetype and PML- type viral strains.


This article has been cited by other articles:

  • Baranek, C., Sock, E., Wegner, M. (2005). The POU protein Oct-6 is a nucleocytoplasmic shuttling protein. Nucleic Acids Res 33: 6277-6286 [Abstract] [Full Text]  
  • Zheng, H.-Y., Takasaka, T., Noda, K., Kanazawa, A., Mori, H., Kabuki, T., Joh, K., Oh-ishi, T., Ikegaya, H., Nagashima, K., Hall, W. W., Kitamura, T., Yogo, Y. (2005). New sequence polymorphisms in the outer loops of the JC polyomavirus major capsid protein (VP1) possibly associated with progressive multifocal leukoencephalopathy. J. Gen. Virol. 86: 2035-2045 [Abstract] [Full Text]  
  • Schlierf, B., Ludwig, A., Klenovsek, K., Wegner, M. (2002). Cooperative binding of Sox10 to DNA: requirements and consequences. Nucleic Acids Res 30: 5509-5516 [Abstract] [Full Text]  
  • Peirano, R. I., Wegner, M. (2000). The glial transcription factor Sox10 binds to DNA both as monomer and dimer with different functional consequences. Nucleic Acids Res 28: 3047-3055 [Abstract] [Full Text]  
  • Okada, Y., Sawa, H., Tanaka, S., Takada, A., Suzuki, S., Hasegawa, H., Umemura, T., Fujisawa, J.-i., Tanaka, Y., Hall, W. W., Nagashima, K. (2000). Transcriptional Activation of JC Virus by Human T-lymphotropic Virus Type I Tax Protein in Human Neuronal Cell Lines. J. Biol. Chem. 275: 17016-17023 [Abstract] [Full Text]  
  • Sock, E., Enderich, J., Wegner, M. (1999). The J Domain of Papovaviral Large Tumor Antigen Is Required for Synergistic Interaction with the POU-Domain Protein Tst-1/Oct6/SCIP. Mol. Cell. Biol. 19: 2455-2464 [Abstract] [Full Text]  
  • Ciappi, S, Azzi, A, De Santis, R, Leoncini, F, Sterrantino, G, Mazzotta, F, Mecocci, L (1999). Archetypal and rearranged sequences of human polyomavirus JC transcription control region in peripheral blood leukocytes and in cerebrospinal fluid. J. Gen. Virol. 80: 1017-1023 [Abstract]  
  • Kuhlbrodt, K., Schmidt, C., Sock, E., Pingault, V., Bondurand, N., Goossens, M., Wegner, M. (1998). Functional Analysis of Sox10 Mutations Found in Human Waardenburg-Hirschsprung Patients. J. Biol. Chem. 273: 23033-23038 [Abstract] [Full Text]  
  • Kuhlbrodt, K., Herbarth, B., Sock, E., Enderich, J., Hermans-Borgmeyer, I., Wegner, M. (1998). Cooperative Function of POU Proteins and SOX Proteins in Glial Cells. J. Biol. Chem. 273: 16050-16057 [Abstract] [Full Text]  
  • Kuhlbrodt, K., Herbarth, B., Sock, E., Hermans-Borgmeyer, I., Wegner, M. (1998). Sox10, a Novel Transcriptional Modulator in Glial Cells. J. Neurosci. 18: 237-250 [Abstract] [Full Text]  
  • Schreiber, J., Enderich, J., Sock, E., Schmidt, C., Richter-Landsberg, C., Wegner, M. (1997). Redundancy of Class III POU Proteins in the Oligodendrocyte Lineage. J. Biol. Chem. 272: 32286-32293 [Abstract] [Full Text]  
  • Ryan, A K, Rosenfeld, M G (1997). POU domain family values: flexibility, partnerships, and developmental codes.. Genes Dev. 11: 1207-1225  
  • Sock, E., Enderich, J., Rosenfeld, M. G., Wegner, M. (1996). Identification of the Nuclear Localization Signal of the POU Domain Protein Tst-1/Oct6. J. Biol. Chem. 271: 17512-17518 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»