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J. Virol., 10 1996, 7331-7334, Vol 70, No. 10
Copyright © 1996, American Society for Microbiology

PCR engineering of viral quasispecies: a new method to preserve and manipulate genetic diversity of RNA virus populations

KM Chumakov
Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852, USA.

A PCR-based method for the controlled manipulation of individual genomic sites of poliovirus with concomitant preservation of the sequence heterogeneity of the rest of the genome is proposed. The new approach can be used for the creation of stable DNA repositories of populations of extremely heterogenous RNA viruses and may have implications for live vaccine technology.

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