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J. Virol., Jan 1996, 667-671, Vol 70, No. 1
Copyright © 1996, American Society for Microbiology

Specific binding of human immunodeficiency virus type 1 (HIV-1) Gag- derived proteins to a 5' HIV-1 genomic RNA sequence

U Geigenmuller and ML Linial
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.

We developed an in vitro binding assay to study the specific interaction between human immunodeficiency virus type 1 (HIV-1) RNA and the Gag polyprotein. Binding of the in vitro-expressed protein to in vitro-transcribed RNA was determined by altered migration of the protein in polyacrylamide gels. We found that a Gag precursor lacking the matrix domain bound specifically to HIV-1 RNA, while deletion of both matrix and capsid domains diminished the specificity of binding. Among several regions of HIV-1 RNA tested, strongest binding was seen with the 5'-most 261 nucleotides, while antisense RNA from the same region did not bind.


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