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J. Virol., Mar 1995, 1959-1963, Vol 69, No. 3
Copyright © 1995, American Society for Microbiology

Characterization of a live, attenuated human parainfluenza type 3 virus candidate vaccine strain

R Ray, K Meyer, FK Newman and RB Belshe
Division of Infectious Diseases and Immunology, Saint Louis University Health Sciences Center, Missouri 63110.

Characterization of a temperature-sensitive and live, attenuated human parainfluenza type 3 virus strain (cp45) grown at a permissive temperature (32 degrees C) suggested that the virus efficiently multiplies in cell lines and retains antigenic and functional properties of the envelope glycoproteins. When grown at a nonpermissive temperature (39.5 degrees C), the cp45 virus exhibited poor replication; however, shifting to a permissive temperature allowed virus growth. Although at a nonpermissive temperature virus polypeptide synthesis was significantly reduced, the hemagglutinin-neuraminidase and fusion glycoproteins were transported to cell surfaces and retained their characteristic biologic activities. Studies on mRNA synthesis from the P protein gene suggested a poor transcriptional activity of the cp45 virus at a nonpermissive temperature. Results from this study indicate that the temperature sensitivity of cp45 virus is related to altered transcriptional activity and a marked reduction in virus polypeptide synthesis.


This article has been cited by other articles:

  • Ray, R. B., Steele, R., Meyer, K., Ray, R. (1997). Transcriptional Repression of p53 Promoter by Hepatitis C Virus Core Protein. J. Biol. Chem. 272: 10983-10986 [Abstract] [Full Text]  


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