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J Virol. 1989 May; 63(5): 2343-2347

Association of p60fyn with middle tumor antigen in murine polyomavirus-transformed rat cells.

I D Horak, T Kawakami, F Gregory, K C Robbins and J B Bolen

Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.

ABSTRACT

Rabbit antisera raised against human FYN-specific peptides were used to evaluate the expression of the fyn gene product in normal and murine polyomavirus middle tumor antigen (MTAg)-transformed rat cells. The antisera were capable of detecting p60fyn in both normal and MTAg-transformed cells. Two different antisera directed against unique p60fyn sequences were found to detect p60fyn-MTAg complexes in cell lysates from the MTAg-transformed cells. The MTAg molecules immunoprecipitated by FYN antisera were phosphorylated on tyrosine during immune-complex kinase reactions at sites similar to those found on MTAg in complexes with pp60c-src. Whereas the abundance of p60fyn was estimated to be less in the MTAg-transformed cells than in their normal counterparts, the specific activities of p60fyn molecules in the normal and transformed cells were similar.

J Virol. 1989 May; 63(5): 2343-2347




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