This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nuebling, C M Articles by Mueller-Lantzsch, N Search for Related Content PubMed PubMed Citation Articles by Nuebling, C M Articles by Mueller-Lantzsch, N

 Previous Article  |  Next Article 

J Virol. 1989 November; 63(11): 4609-4615

Identification and characterization of an Epstein-Barr virus early antigen that is encoded by the NotI repeats.

C M Nuebling and N Mueller-Lantzsch

Institut für Mikrobiologie und Hygiene, Abteilung Virologie, Freiburg, Federal Republic of Germany.

ABSTRACT

The Epstein-Barr virus (EBV) genome is characterized by two regions carrying partially homologous clusters of short tandem repeats (NotI and PstI repeats) flanked by 1,044 and 1,045 base pairs with almost perfect homology (DL and DR, left and right duplications, respectively). Both repetitive regions are transcribed into poly(A)+ mRNA after induction of the productive EBV cycle with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate and contain open reading frames. To identify the potential protein encoded by the NotI repeat open reading frame (BHLF1), two repeat units of EBV strain M-ABA were expressed using the tryptophan-regulated Escherichia coli expression vector pATH11. Rabbit antisera generated against the resulting fusion protein reacted specifically with a protein varying in molecular size between 70,000 and 90,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, found after 12-O-tetradecanoyl-phorbol-13-acetate or n-butyrate induction in various cell lines harboring EBV. In immunofluorescence tests with the BHLF1-specific antiserum, an immunofluorescence with EA-D specificity could be observed. In addition, the BHLF1 protein is exhibiting polyanion-binding activity with a maximum for single-stranded DNA. Furthermore, the fusion protein is recognized by a number of human EBV-positive sera.

---

J Virol. 1989 November; 63(11): 4609-4615

This article has been cited by other articles:

• Ikuta, K., Srinivas, S. K., Schacker, T., Miyagi, J.-i., Scott, R. S., Sixbey, J. W. (2008). Points of Recombination in Epstein-Barr Virus (EBV) Strain P3HR-1-Derived Heterogeneous DNA as Indexes to EBV DNA Recombinogenic Events In Vivo. J. Virol. 82: 11516-11525 [Abstract] [Full Text]  
• Xue, S.-A., Griffin, B. E. (2007). Complexities associated with expression of Epstein-Barr virus (EBV) lytic origins of DNA replication. Nucleic Acids Res 35: 3391-3406 [Abstract] [Full Text]  
• Xue, S.-A., Jones, M. D., Lu, Q.-L., Middeldorp, J. M., Griffin, B. E. (2003). Genetic Diversity: Frameshift Mechanisms Alter Coding of a Gene (Epstein-Barr Virus LF3 Gene) That Contains Multiple 102-Base-Pair Direct Sequence Repeats. Mol. Cell. Biol. 23: 2192-2201 [Abstract] [Full Text]  
• Xue, S.-A., Lu, Q.-L., Poulsom, R., Karran, L., Jones, M. D., Griffin, B. E. (2000). Expression of Two Related Viral Early Genes in Epstein-Barr Virus-Associated Tumors. J. Virol. 74: 2793-2803 [Abstract] [Full Text]  
• Steven, N.M., Annels, N.E., Kumar, A., Leese, A.M., Kurilla, M.G., Rickinson, A.B. (1997). Immediate Early and Early Lytic Cycle Proteins Are Frequent Targets of the Epstein-Barr Virus-induced Cytotoxic T Cell Response. JEM 185: 1605-1618 [Abstract] [Full Text]