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J Virol. 1970 May; 5(5): 639-650
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Replication of Sendai Virus

II. Steps in Virus Assembly

Virus Laboratory, University of California, Berkeley, California 94720
Department of Medicine, Stanford University, Stanford, California 94305

ABSTRACT

Chick embryo fibroblast cultures infected with Sendai virus were incubated with ³H-uridine in the presence of actinomycin D beginning at 18 hr after infection. The 35 and 18S virus-specific ribonucleic acid (RNA) components were found in a ribonuclease-sensitive form in the cell and appeared to be associated with polyribosomes. Newly synthesized 57S viral RNA was rapidly coated with protein to form intracellular viral nucleocapsid, and no 57S RNA was found "free" (ribonucleasesensitive) in the 2,000 x g supernatant fraction of disrupted cells. The nucleocapsid from detergent-disrupted Sendai virus and that from disrupted cells were indistinguishable in ultrastructure and buoyant density, and neither was found to be infectious or have hemagglutinating activity. Kinetic studies of nucleocapsid and virus formation indicated a relative block in conversion of viral nucleocapsid to complete enveloped virus in these cells, resulting in accumulation of large amounts of nucleocapsid in the cell cytoplasm.

¹ Part of this work was taken from a thesis submitted by C. D. B. in partial satisfaction of the requirements for the Doctor of Philosophy degree from the University of California, Berkeley.