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J Virol. 1970 April; 5(4): 502-506
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Early Intracellular Events in the Replication of Bacteriophage T4 Deoxyribonucleic Acid

VI. Newly Synthesized Proteins in the T4 Protein-Deoxyribonucleic Acid Complex ¹

Department of Medical Genetics, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

Shortly after infection of Escherichia coli B with T4 phage, the phage deoxyribonucleic acid (DNA) can be isolated as a fast-sedimenting, proteinaceous complex. Formation of the complex is inhibited by the addition of chloramphenicol between 3 and 4 min after infection, suggesting that phage-coded proteins are necessary to form the complex and may contribute to its structure. To determine whether the phage DNA is associated with a random collection of proteins after infection or whether the complex contained a specific set of proteins, total protein from phage-infected cell lysates was compared to complex protein isolated from similar lysates by gel acrylamide electrophoresis. The proteins obtained from complexes exhibited a distinctly different pattern of separation, indicating that the complex contained a specific set of those proteins newly synthesized after infection. The proteins of the complex appear to be associated directly with the DNA rather than with some other component which could impart the characteristic of fast sedimentation to the complex. Fast-sedimenting complexes were isolated from a ³H-leucine-labeled cell lysate. Part of this material was treated with pancreatic deoxyribonuclease. Deoxyribonuclease-treated and untreated complexes were resedimented in sucrose gradients. Virtually all the untreated complex remained fast-sedimenting, whereas most of the ³H-leucine label of the deoxyribonuclease-treated material was located toward the top of the gradient. These data suggest a direct association of DNA and protein in the complex.

² Present address: Institute for Enzyme Research, University of Wisconsin, Madison, Wis. 53706.

¹ Submitted by Robert C. Miller, Jr., in partial fulfillment of the requirements for the Ph.D. degree from the Department of Molecular Biology, University of Pennsylvania, Philadelphia.