This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Newbold, J. E. Articles by Sinsheimer, R. L. Search for Related Content PubMed PubMed Citation Articles by Newbold, J. E. Articles by Sinsheimer, R. L.

 Previous Article  |  Next Article 

J Virol. 1970 April; 5(4): 427-431
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Process of Infection with Bacteriophage X174

XXXIV. Kinetics of the Attachment and Eclipse Steps of the Infection

Division of Biology, California Institute of Technology, Pasadena, California 91109

ABSTRACT

The products of X cistrons II, III, and VII are demonstrated to affect the attachment of the phage to its host Escherichia coli C; therefore, by inference, these cistrons influence, directly or indirectly, the structure of proteins in the virus particle. Two of the mutations which alter attachment kinetics, ts79 in cistron III and h in cistron VII, also affect the electrophoretic mobility of the virus and emphasize the role of charge in the attachment interaction with the host. The kinetics for attached phage to go into "eclipse" are first-order and biphasic; about 85% of the phage eclipse at one rate (k_e = 0.86 min^–1) and the remainder do so at a distinctly lower rate (k_e = 0.21 min^–1). No X cistrons yet identified affect the eclipse process. The lowest temperature at which eclipse is detected is 19 C. The Arrhenius activation energy for phage eclipse has the high value of 36.6 kcal/mole, indicating the cooperative nature of the event.

This article has been cited by other articles:

• Everson, J. S., Garner, S. A., Fane, B., Liu, B.-L., Lambden, P. R., Clarke, I. N. (2002). Biological Properties and Cell Tropism of Chp2, a Bacteriophage of the Obligate Intracellular Bacterium Chlamydophila abortus. J. Bacteriol. 184: 2748-2754 [Abstract] [Full Text]