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J Virol. 1970 January; 5(1): 45-50
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Multiplication of Bacteriophage P22 in Penicillin-Induced Spheroplasts of Salmonella typhimurium

Department of Biochemistry and Biophysics, College of Medical Sciences, Banaras Hindu University, Varanasi-5, U.P., India

ABSTRACT

The spheroplasts of Salmonella typhimurium (LT2) prepared by treatment with penicillin were capable of adsorbing phage P22 C₁. The normal multiplication of the phage took place, although the burst size was reduced to one-fourth of that in intact cells. Rate of incorporation of ¹⁴C-thymidine into spheroplasts was increased severalfold on phage infection. Multiplication of C⁺ also took place, but no lysogeny could be established in spheroplasts. Furthermore, spheroplasts prepared from cells lysogenized with wild-type phage, LT2 (C⁺), and a temperature-inducible C₂ mutant, LT2(tsC₂), were not inducible. Unlike normal cells, both mitomycin C and actinomycin D interfered with the phage multiplication in spheroplasts. The spheroplast system offers great advantages in the study of the synthesis of nucleic acids and proteins in phage-infected LT2.

¹ Senior Research Fellow under a research program sponsored by the Council of Scientific and Industrial Research, New Delhi, India.

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