This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tamashiro, J C Articles by Spector, D H Search for Related Content PubMed PubMed Citation Articles by Tamashiro, J C Articles by Spector, D H

 Previous Article  |  Next Article 

J Virol. 1982 May; 42(2): 547-557

Construction of a cloned library of the EcoRI fragments from the human cytomegalovirus genome (strain AD169).

ABSTRACT

The DNA genome of human cytomegalovirus (HCMV) strain AD169 is 158 x 10(6) Mr. Cleavage of the HCMV DNA with the restriction endonuclease EcoRI yields 35 major fragments ranging in size from 0.54 x 10(6) Mr. We have constructed a cloned library of the EcoRI fragments of this strain of HCMV, using the plasmid pACYC184 and the recipient bacterium Escherichia coli strain HB101 RecA-. The viral origin of the cloned inserts was determined by hybridization to viral DNA. The fragments were characterized further by digestion with other restriction enzymes. Several clones were obtained which contained sequences spanning the junction between the long (L) and short (S) components of the viral DNA sequences. These clones differed in molecular weight by multiples of 0.3 x 10(6) to 0.4 x 10(6) Mr. The variability found in the clones was also reflected in the genome. Each clone containing a junction sequence hybridized to a series of bands on Southern filters of EcoRI-digested HCMV DNA. This "ladder effect" provided evidence for a region of heterogeneity within the L-S junction.

This article has been cited by other articles:

• Luo, M. H., Schwartz, P. H., Fortunato, E. A. (2008). Neonatal Neural Progenitor Cells and Their Neuronal and Glial Cell Derivatives Are Fully Permissive for Human Cytomegalovirus Infection. J. Virol. 82: 9994-10007 [Abstract] [Full Text]  
• Sanders, R. L., Clark, C. L., Morello, C. S., Spector, D. H. (2008). Development of Cell Lines That Provide Tightly Controlled Temporal Translation of the Human Cytomegalovirus IE2 Proteins for Complementation and Functional Analyses of Growth-Impaired and Nonviable IE2 Mutant Viruses. J. Virol. 82: 7059-7077 [Abstract] [Full Text]  
• Tran, K., Mahr, J. A., Choi, J., Teodoro, J. G., Green, M. R., Spector, D. H. (2008). Accumulation of Substrates of the Anaphase-Promoting Complex (APC) during Human Cytomegalovirus Infection Is Associated with the Phosphorylation of Cdh1 and the Dissociation and Relocalization of APC Subunits. J. Virol. 82: 529-537 [Abstract] [Full Text]  
• Kapasi, A. J., Spector, D. H. (2008). Inhibition of the Cyclin-Dependent Kinases at the Beginning of Human Cytomegalovirus Infection Specifically Alters the Levels and Localization of the RNA Polymerase II Carboxyl-Terminal Domain Kinases cdk9 and cdk7 at the Viral Transcriptosome. J. Virol. 82: 394-407 [Abstract] [Full Text]  
• Luo, M. H., Fortunato, E. A. (2007). Long-Term Infection and Shedding of Human Cytomegalovirus in T98G Glioblastoma Cells. J. Virol. 81: 10424-10436 [Abstract] [Full Text]  
• White, E. A., Del Rosario, C. J., Sanders, R. L., Spector, D. H. (2007). The IE2 60-Kilodalton and 40-Kilodalton Proteins Are Dispensable for Human Cytomegalovirus Replication but Are Required for Efficient Delayed Early and Late Gene Expression and Production of Infectious Virus. J. Virol. 81: 2573-2583 [Abstract] [Full Text]  
• Shlapobersky, M., Sanders, R., Clark, C., Spector, D. H. (2006). Repression of HMGA2 Gene Expression by Human Cytomegalovirus Involves the IE2 86-Kilodalton Protein and Is Necessary for Efficient Viral Replication and Inhibition of Cyclin A Transcription.. J. Virol. 80: 9951-9961 [Abstract] [Full Text]  
• Casavant, N. C., Luo, M. H., Rosenke, K., Winegardner, T., Zurawska, A., Fortunato, E. A. (2006). Potential Role for p53 in the Permissive Life Cycle of Human Cytomegalovirus.. J. Virol. 80: 8390-8401 [Abstract] [Full Text]  
• Sanchez, V., Spector, D. H. (2006). Cyclin-Dependent Kinase Activity Is Required for Efficient Expression and Posttranslational Modification of Human Cytomegalovirus Proteins and for Production of Extracellular Particles.. J. Virol. 80: 5886-5896 [Abstract] [Full Text]  
• Tamrakar, S., Kapasi, A. J., Spector, D. H. (2005). Human Cytomegalovirus Infection Induces Specific Hyperphosphorylation of the Carboxyl-Terminal Domain of the Large Subunit of RNA Polymerase II That Is Associated with Changes in the Abundance, Activity, and Localization of cdk9 and cdk7. J. Virol. 79: 15477-15493 [Abstract] [Full Text]  
• White, E. A., Spector, D. H. (2005). Exon 3 of the Human Cytomegalovirus Major Immediate-Early Region Is Required for Efficient Viral Gene Expression and for Cellular Cyclin Modulation. J. Virol. 79: 7438-7452 [Abstract] [Full Text]  
• Sanchez, V., McElroy, A. K., Yen, J., Tamrakar, S., Clark, C. L., Schwartz, R. A., Spector, D. H. (2004). Cyclin-Dependent Kinase Activity Is Required at Early Times for Accurate Processing and Accumulation of the Human Cytomegalovirus UL122-123 and UL37 Immediate-Early Transcripts and at Later Times for Virus Production. J. Virol. 78: 11219-11232 [Abstract] [Full Text]  
• Rosenke, K., Fortunato, E. A. (2004). Bromodeoxyuridine-Labeled Viral Particles as a Tool for Visualization of the Immediate-Early Events of Human Cytomegalovirus Infection. J. Virol. 78: 7818-7822 [Abstract] [Full Text]  
• White, E. A., Clark, C. L., Sanchez, V., Spector, D. H. (2004). Small Internal Deletions in the Human Cytomegalovirus IE2 Gene Result in Nonviable Recombinant Viruses with Differential Defects in Viral Gene Expression. J. Virol. 78: 1817-1830 [Abstract] [Full Text]  
• Biswas, N., Sanchez, V., Spector, D. H. (2003). Human Cytomegalovirus Infection Leads to Accumulation of Geminin and Inhibition of the Licensing of Cellular DNA Replication. J. Virol. 77: 2369-2376 [Abstract] [Full Text]  
• Fortunato, E. A., Sanchez, V., Yen, J. Y., Spector, D. H. (2002). Infection of Cells with Human Cytomegalovirus during S Phase Results in a Blockade to Immediate-Early Gene Expression That Can Be Overcome by Inhibition of the Proteasome. J. Virol. 76: 5369-5379 [Abstract] [Full Text]  
• Sanchez, V., Clark, C. L., Yen, J. Y., Dwarakanath, R., Spector, D. H. (2002). Viable Human Cytomegalovirus Recombinant Virus with an Internal Deletion of the IE2 86 Gene Affects Late Stages of Viral Replication. J. Virol. 76: 2973-2989 [Abstract] [Full Text]  
• McElroy, A. K., Dwarakanath, R. S., Spector, D. H. (2000). Dysregulation of Cyclin E Gene Expression in Human Cytomegalovirus-Infected Cells Requires Viral Early Gene Expression and Is Associated with Changes in the Rb-Related Protein p130. J. Virol. 74: 4192-4206 [Abstract] [Full Text]  
• Fortunato, E. A., Dell'Aquila, M. L., Spector, D. H. (2000). Specific chromosome 1 breaks induced by human cytomegalovirus. Proc. Natl. Acad. Sci. USA 97: 853-858 [Abstract] [Full Text]  
• Rodems, S. M., Spector, D. H. (1998). Extracellular Signal-Regulated Kinase Activity Is Sustained Early during Human Cytomegalovirus Infection. J. Virol. 72: 9173-9180 [Abstract] [Full Text]  
• Rodems, S. M., Clark, C. L., Spector, D. H. (1998). Separate DNA Elements Containing ATF/CREB and IE86 Binding Sites Differentially Regulate the Human Cytomegalovirus UL112-113 Promoter at Early and Late Times in the Infection. J. Virol. 72: 2697-2707 [Abstract] [Full Text]