This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beemon, K Articles by Sefton, B M Search for Related Content PubMed PubMed Citation Articles by Beemon, K Articles by Sefton, B M

 Previous Article  |  Next Article 

J Virol. 1979 April; 30(1): 190-200

Polymorphism of avian sarcoma virus src proteins.

K Beemon, T Hunter and B M Sefton

ABSTRACT

The src gene products of seven different avian sarcoma viruses were compared. In vitro translation of virion RNA yielded products identified unambiguously as p60src in the case of two stocks of the Schmidt-Ruppin strain, three stocks of the Prague strain, the Bryan strain, and the Bratislava 77 strain of avian sarcoma virus. Differences in the electrophoretic mobility of these seven p60src proteins in sodium dodecyl sulfate-polyacrylamide gels, corresponding to variation in the apparent molecular weights ranging from 56,000 to 60,500, were observed. Antigenic variability was also found; only three of the seven viruses tested encoded a p60src, which was precipitated by antisera derived from rabbits bearing tumors induced by the Schmidt-Ruppin strain of Rous sarcoma virus. Examination of the methionine-containing tryptic peptides of the seven ;60src proteins by two-dimensional mapping revealed four common peptides but marked variability in the five to eight other peptides in each protein. Clear differences in the peptide maps of p60src were observed, both between different strains of virus and within strains. In the three cases examined, p60src synthesized in transformed cells was found to be essentially identical to that synthesized in vitro. We conclude that there is significant polymorphism in the p60src proteins of the avian sarcoma viruses.

---

J Virol. 1979 April; 30(1): 190-200

This article has been cited by other articles:

• Duesberg, P. H. (1980). Transforming Genes of Retroviruses. Cold Spring Harb Symp Quant Biol 44: 13-29 [Abstract]  
• Wang, L.-H., Snyder, P., Hanafusa, T., Moscovici, C., Hanafusa, H. (1980). Comparative Analysis of Cellular and Viral Sequences Related to Sarcomagenic Cell Transformation. Cold Spring Harb Symp Quant Biol 44: 755-764 [Abstract]  
• Hunter, T., Sefton, B. M., Beemon, K. (1980). Studies on the Structure and Function of the Avian Sarcoma Virus Transforming-gene Product. Cold Spring Harb Symp Quant Biol 44: 931-941 [Abstract]  
• Radke, K., Martin, G. S. (1980). Transformation by Rous Sarcoma Virus: Effects of src-gene Expression on the Synthesis and Phosphorylation of Cellular Polypeptides. Cold Spring Harb Symp Quant Biol 44: 975-982 [Abstract]