This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Smith, H. S. Articles by Riggs, J. Search for Related Content PubMed PubMed Citation Articles by Smith, H. S. Articles by Riggs, J.

 Previous Article  |  Next Article 

J Virol. 1976 January; 17(1): 269-274
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Studies on Morphological Transformation of BALB/3T3-Derived Clones by Murine Leukemia Virus

^* Cell Culture Laboratory, School of Public Health, University of California-Berkeley, Berkeley, California 94720
California State Department of Public Health Viral and Rickettsial Disease Laboratory, Berkeley, California 94720

ABSTRACT

We have described a cell line, UC1-B, derived spontaneously from BALB/3T3 mouse embryo cells, which, unlike the standard BALB/3T3, are morphologically transformed and produce bizarre viral forms in response to murine leukemia virus. Although UC1-B and BALB/3T3 are morphologically similar, and both form contact-inhibited monolayers at confluence, the UC1-B cells are partially transformed because: they grow to a slightly higher saturation density than 3T3 cells, they grow in medium lacking serum growth factors, and they produce tumors in mice. Another clone, 12A-3, derived from BALB/3T3, also transforms and produces bizarre viral forms after infection with murine leukemia virus. Unlike UC1-B cells, the 12A3-8 cells are identical in growth properties to BALB/3T3; therefore, a partially altered morphology is not required for the induction of transformation by murine leukemia virus.