This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beard, P. Articles by Maxwell, I. H. Search for Related Content PubMed PubMed Citation Articles by Beard, P. Articles by Maxwell, I. H.

 Previous Article  |  Next Article 

J Virol. 1976 January; 17(1): 20-26
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Strand-Specific Transcription of Polyoma Virus DNA Early in Productive Infection and in Transformed Cells

Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
Department of Molecular Biology, University of Geneva, Geneva, Switzerland

ABSTRACT

The DNA strand origin of nuclear and cytoplasmic polyoma-specific RNA in productively infected mouse cells and in a line of polyoma-transformed hamster cells was determined by hybridization of unlabeled RNA with radioactively labeled separated strands of polyoma DNA. Early in the productive cycle (10 h postinfection) nuclear viral RNA is complementary to only about 40% of the E strand of viral DNA. No RNA complementary to the L strand was detected even when the RNA was first self-annealed to enrich for possible minor species. Early cytoplasmic RNA is complementary to the same 40% of the E strand. Thus, only that part of the polyoma genome which codes for early viral messenger RNA appears to be transcribed. Late in infection, nuclear viral RNA is complementary to most or all of the L strand and to at least 60% of the E strand. Late cytoplasmic viral RNA hybridizes to 40 to 45% of the E strand and 50 to 55% of the L strand. The transformed cell nuclear viral RNA is complementary to 60% of the E strand, whereas cytoplasmic RNA is complementary to 40% of the E strand and comprises the same polyoma-specific sequences as are found in RNA early in productive infection. No L strand transcripts could be detected. Thus, in the transformed cells and late in productive infection, viral RNA sequences in the cytoplasm are a specific subset of those in the nucleus.

¹ Present address: Department of Anatomy, University of Colorado, School of Medicine, Denver, Col. 80220.

This article has been cited by other articles:

• Kumar, M., Carmichael, G. G. (1998). Antisense RNA: Function and Fate of Duplex RNA in Cells of Higher Eukaryotes. Microbiol. Mol. Biol. Rev. 62: 1415-1434 [Abstract] [Full Text]  
• Kumar, M., Carmichael, G. G. (1997). Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts. Proc. Natl. Acad. Sci. USA 94: 3542-3547 [Abstract] [Full Text]