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J Virol. 1974 November; 14(5): 1070-1075
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Mechanism of Replication of X174 Single-Stranded DNA IX. Requirement for the Escherichia coli dnaG Protein

¹ Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, Quebec, Canada H3G 1Y6

ABSTRACT

Escherichia coli NY73, possessing a temperature-sensitive mutation in the dnaG locus, was rendered sensitive to bacteriophage X174 by P1 transduction. X174 reproduces in this strain at 30 C but not at 40 C. All three stages of X174 replication, parental replicative form (RF) synthesis, RF replication, and progeny single-stranded DNA synthesis, are thermolabile in this mutant. Competition-annealing data show that both plus- and minus-strand synthesis are equally inhibited after shift up to 40 C during RF replication. We conclude that the dnaG gene product is required for the synthesis of both strands of X RF during RF replication and of the complementary strand and viral progeny strands during stages I and III, respectively.