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J Virol. 1974 October; 14(4): 840-845
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Effect of ß-Propiolactone Inactivation of Polyoma Virus on Viral Functions

^a Department of Molecular Biology, University of Geneva, Geneva, Switzerland

ABSTRACT

Polyoma virus was inactivated by treatment with ß-propiolactone. T-antigen production, polyoma-RNA synthesis, induction of host DNA synthesis (measured by incorporation of labeled thymidine into the cell culture), and in vitro transforming ability were inactivated to a similar degree by various ß-propiolactone concentrations (0.25% ß-propiolactone reduced these functions approximately 96%), whereas plaque-forming ability and the ability of the virus to replicate its DNA and to synthesize capsid antigen were inactivated by a given concentration of ß-propiolactone to a much greater degree (0.25% ß-propiolactone led to a reduction of plaque-forming ability of over 8 logs). The significance of these data and their relationship to previously published experiments are discussed.

¹ Present address: Department of Medicine, USVA Hospital, University Dr. C, Pittsburgh, Pa. 15240.

² Permanent address: Division of Biology, Kansas State University, Manhattan, Kan. 66506.

³ Present address: Department of Medicine, Stanford University Medical Center, Stanford, Calif. 94305.