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J Virol. 1974 June; 13(6): 1263-1273
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Simian Virus 40 Transcription in Productively Infected and Transformed Cells

¹ Center for Cancer Research and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Department of Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel
Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514

ABSTRACT

Several independent cell lines transformed by simian virus 40 carry a species of viral RNA of 900,000 to 1,000,000 daltons. A viral RNA species of similar size is found early in the lytic cycle. Late in the viral lytic cycle, two prominent viral RNA species of about 600,000 and 900,000 daltons are seen. The larger late species shares nucleotide sequences with, and is less stable than, the smaller. These RNA species are located in the cytoplasm of the infected cell. The regions of the viral genome coding for these RNA species are mapped by hybridization of lytic RNA species to fragments of the genome produced by cleavage with Haemophilus aegyptius endonuclease.