Files in this Data Supplement:

• Supplementary file 1 - Table S1 (Sequence comparison between Mamu-A*02 binding epitopes from SIVmac239 and the same epitopes in SIVsmE660.)
  Table S2 (Frequency and breadth of vaccine-induced cellular immune responses measured in PBMC and CD8-depleted PBMC prior to initiation of intrarectal challenges.)
  Legends to Fig. S1-S7
  Zipped Word file, 76K.
• Supplementary file 2 - Fig. S1 (Western blot at 8 weeks postvaccination.)
  Zipped EPS file, 2.3MB.
• Supplementary file 3 - Fig. S2 (Titration of SIVsmE660 stock by mucosal challenge to determine the dose at which 3 or fewer viral variants cross the mucosal barrier.)
  Zipped TIFF file, 604K.
• Supplementary file 4 - Fig. S3 (Absolute CD4 and CD4 total memory counts.)
  Zipped EPS file, 1.9MB.
• Supplementary file 5 - Fig. S4 (Anamnestic vaccine-induced immune responses in the vaccinees.)
  Zipped TIFF file, 568K.
• Supplementary file 6 - Fig. S5 (Viral loads of naive animals challenged intravenously with the same stock of SIVsmE660 as was used in the experiments described in the article.)
  Zipped EPS file, 145K.
• Supplementary file 7 - Fig. S6 (Viral loads of SIVmac239ΔNef-vaccinated animals challenged intravenously with the same stock of SIVsmE660 as was used in the experiments described in the article.)
  Zipped EPS file, 187K.
• Supplementary file 8 - Fig. S7 (Viral loads of naive animals challenged intrarectally with the same stock of SIVsmE660 as was used in the experiments described in the article.)
  Zipped EPS file, 112K.