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The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles
J. Virol. Siu et al. 82: 11318

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Video S1 (Video corresponding to Fig 6B. Live cell imaging on Vero E6 cells that produce M-E-NeGFP SARS-CoV virus-like particles.)
    Zipped QuickTime video (.mov) file, 8.1 MB.
  • Supplemental file 2 - Video S2 (Video corresponding to Fig. 6C,h. Cells were treated overnight with BFA, BFA was washed out, and image sequence acquired.)
    Zipped QuickTime video (.mov) file, 18.4 MB.
  • Supplemental file 3 - Video S3 (Video corresponding to Fig. 6C,b. Cells were incubated overnight without BFA, BFA was added, and image sequence acquired at 25 min post-addition for 45 min.)
    Zipped QuickTime video (.mov) file, 10.3 MB.
  • Supplemental file 4 - Video S4 (Video corresponding to Fig. 6C,i. Cells were treated overnight with BFA, BFA was washed out, and image sequence acquired at 3 h post-wash for 10 min.)
    Zipped QuickTime video (.mov) file, 15.4 MB.
  • Supplemental file 5 - Video S5 (Video corresponding to Fig. 6C,f. Cells were incubated overnight without BFA, BFA was added for 4 h and washed out for 2 h.)
    Zipped QuickTime video (.mov) file, 13.8 MB.




This Article
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