Files in this Data Supplement:

• Supplemental file 1 - Fig. S1 (EM pictures of IMV bound to actin protrusions on HeLa cells.)
  Fig. S2 (EM of IMV bound to actin protrusions on HeLa cells.)
  Fig. S3 (Anti-M2 antibodies specifically recognized VPEF protein but not vaccinia IMV in immunofluorescence analyses.)
  Fig. S4 (Soluble VPEF protein and siRNA targeting VPEF blocked vaccinia IMV penetration into HeLa cells.)
  PDF file, 5.3MB.
• Supplemental file 2 - Movie S1 (mCherry-VV moved along actin protrusions before reaching HeLa cell bodies.)
  Zipped MOV file, 99K.
• Supplemental file 3 - Movie S2 (mCherry-VV moved along actin protrusions before reaching HeLa cell bodies.)
  Zipped MOV file, 145K.
• Supplemental file 4 - Movie S3 (mCherry-VV moved in an active ruffling area.)
  Zipped MOV file, 134K.
• Supplemental file 5 - Movie S4 (mCherry-VV was grabbed by waving actin protrusions.)
  Zipped MOV file, 348K.
• Supplemental file 6 - Movie S5. IMV movement is actin dependent.
  Zipped MOV file, 147K.
• Supplemental file 7 - Movie S6. IMV movement is not affected by taxol treatment.
  Zipped MOV file, 118K.