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Journal of Virology, February 2007, p. 1773-1785, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01316-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Physiological Levels of Virion-Associated Human Immunodeficiency Virus Type 1 Envelope Induce Coreceptor-Dependent Calcium Flux ^,

Marta Melar,^1,2 David E. Ott,³ and Thomas J. Hope^1*

Department of Cell and Molecular Biology, Northwestern University, Chicago, Illinois 60611,¹ Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois 60612,² Retroviral Assembly Laboratory, SAIC-Frederick, NCI at Frederick, Frederick, Maryland 21702³

Received 22 June 2006/ Accepted 15 November 2006

Top Abstract Introduction Materials and Methods Results Discussion References

 
Human immunodeficiency virus (HIV) entry into target cells requires the engagement of receptor and coreceptor by envelope glycoprotein (Env). Coreceptors CCR5 and CXCR4 are chemokine receptors that generate signals manifested as calcium fluxes in response to binding of the appropriate ligand. It has previously been shown that engagement of the coreceptors by HIV Env can also generate Ca²⁺ fluxing. Since the sensitivity and therefore the physiological consequence of signaling activation in target cells is not well understood, we addressed it by using a microscopy-based approach to measure Ca²⁺ levels in individual CD4⁺ T cells in response to low Env concentrations. Monomeric Env subunit gp120 and virion-bound Env were able to activate a signaling cascade that is qualitatively different from the one induced by chemokines. Env-mediated Ca²⁺ fluxing was coreceptor mediated, coreceptor specific, and CD4 dependent. Comparison of the observed virion-mediated Ca²⁺ fluxing with the exact number of viral particles revealed that the viral threshold necessary for coreceptor activation of signaling in CD4⁺ T cells was quite low, as few as two virions. These results indicate that the physiological levels of virion binding can activate signaling in CD4⁺ T cells in vivo and therefore might contribute to HIV-induced pathogenesis.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Entry of human immunodeficiency virus type 1 (HIV-1) into target cells requires an interaction of envelope glycoprotein (Env) with CD4 and sequentially with one of the coreceptors, CXCR4 or CCR5 (24, 33, 41, 53, 61, 72). Interestingly, all three proteins have signaling functions in healthy, uninfected cells. CD4 provides a costimulatory signal in antigen-specific T-cell receptor-major histocompatibility complex II interaction in T cells (27). CXCR4 and CCR5 are chemokine receptors coupled to heterotrimeric G_iß proteins. Activation of these G-protein coupled receptors (GPCR) by chemokine ligands initiates signal transduction pathways. These lead to elevation of intracellular free calcium, downregulation of cyclic AMP, activation of phospholipase C, phosphoinositide kinase-3, and mitogen-activated protein kinase pathways (25, 32, 48, 57, 65). The signaling eventually results in upregulation of cytokine production, actin cytoskeleton reorganization, and chemotaxis.

Multiple groups have reported that HIV-1 Env and its subunit gp120 mediate similar changes in cells expressing CD4 and the corresponding coreceptor: Ca²⁺ fluxing (1, 7, 10, 47, 71), cyclic AMP downregulation (31), activation of the mitogen-activated protein kinase MEK/ERK pathway (10, 56), rearrangements of actin cytoskeleton (10, 55), and chemotaxis (35). However, it is not known whether Env binding to its receptors mimics the chemokine-induced signal or creates a unique one and, more importantly, what physiological role it would have in HIV-caused pathogenesis. There are two schools of thought relating to the potential role of HIV envelope based signaling: one that emphasizes the importance of HIV-1 Env's "choice" to engage signaling molecules for its entry (1, 7, 31, 37, 58, 65) and another that suggests that Env-mediated signaling is dispensable for infection (3-5, 8). However, both views do agree on the existence of the Env-mediated signaling phenomenon. The key issue in this debate is the sensitivity of the signaling transduction system. If Env-mediated signaling requires high concentrations of gp120 that are rarely achieved in vivo, it seems unlikely that experimentally detected signaling would be physiologically relevant. In contrast, if low concentrations of gp120 can stimulate signaling, it seems likely that such interaction is highly specific, physiologically relevant, and better reflects the situation found in infected patients. We therefore decided to study Env-mediated signaling specificity, quality, and potency at low concentrations in individual cells.

We used the response of GPCR signaling, intracellular Ca²⁺ elevation, as an indicator of signal initiation. Observing the kinetics of this Ca²⁺ mobilization in individual cells allowed us to dissect the nature of the signal, involvement of CD4, and minimal Env concentration needed to activate signaling within individual primary unstimulated CD4⁺ T cells. This way, we indirectly addressed the question of the physiological relevance of Env-mediated signaling in primary target cells. Indeed, we found that approximately 10 pM of virion-bound gp120 was enough to initiate signaling. This concentration is in a range of gp120 values proposed to be present in the sera of infected patients (29), even though a much higher gp120 concentration is potentially achieved at the sites of infection, the secondary lymphoid organs (62). Translated to the level of an individual cell, we demonstrate signaling initiation with as little as two virions and an average of four virions. Our data reveal that the interaction of a few virions with T cells ex vivo can induce a unique signaling cascade in the absence of infection and in this way potentially contribute to the pathology of AIDS.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Cell lines. CHO-745/CycT CD4⁺, 745/CycT CD4⁺ CCR5⁺, and 745/CycT CD4⁺ CXCR4⁺ cell lines were kindly provided by Paul Bieniasz (The Rockefeller University, New York, NY). Cells were maintained in Iscove's modified Dulbecco medium (Bio-Whittaker/Cambrex Co., Walkersville, MD) containing penicillin G (200 U/ml), gentamicin (10 µg/ml), L-glutamine (0.3 µg/ml), 10% fetal bovine serum (FBS), and 200 µg of G418/ml.

Primary CD4⁺ and CD8⁺ T-cell isolation. Peripheral blood mononuclear cells from healthy blood donors were isolated by Ficoll density gradient centrifugation. The peripheral blood mononuclear cell fraction was monocyte depleted by magnetically labeling cells with anti-CD14 monoclonal antibody (MAb)-coated microbeads and by applying the cell suspension onto a column within the magnetic cell-sorting separator according to the manufacturer's instructions (Miltenyi Biotec, Auburn, CA). Labeled CD14⁺ cells were retained in the magnetized column, and the CD14^– flowthrough was positively selected for CD4⁺ or CD8⁺ T cells by using anti-CD4 or anti-CD8 MAb-coated microbeads, respectively. Subsequently, CD4⁺ or CD8⁺ T cells retained in the magnetized column were eluted when the column was removed from the magnetic field. For some experiments, CD4⁺ T cells were isolated by negative selection using a CD4⁺ T-cell isolation kit (Miltenyi Biotec), where untouched CD4⁺ T cells were collected as a flowthrough after the magnetic separation and all non-CD4⁺ T cells were retained in the column. Both ways of cell isolation yielded 98% CD4⁺ or CD8⁺ T-cell populations, as confirmed by flow cytometry. It is worth noting that there was no difference in the results obtained from CD4⁺ T cells isolated using either the positive or the negative selection method (data not shown). A total of 2 x 10⁶ T cells/ml were cultivated in 15% FBS-RPMI 1640 (Bio-Whittaker/Cambrex) for 24 h without activation and then subjected to fluxing analysis. Work with human tissues was approved by Northwestern University (IRB 1754-002).

Reagents. Stromal cell-derived factor 1 (SDF1) was purchased from Sigma Aldrich (St. Louis, MO). Recombinant monomeric HIV_HXB envelope glycoprotein was kindly provided by Robert W. Doms (University of Pennsylvania). HIV_BaL, AMD3100, and recombinant soluble CD4-183 (sCD4; 2-domain) (28) were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Disease, National Institutes of Health. The anti-p24 MAb AG3 was kindly provided by Jonathan Allan. Anti-hCCR5 and anti-hCXCR4 MAbs were purchased from R&D Systems (Minneapolis, MN), and phycoerythrin-conjugated anti-hCD4 antibody was obtained from Pharmingen, Inc. (San Diego, CA). Cy5 and fluorescein isothiocyanate anti-mouse secondary antibodies were purchased from Jackson Immunoresearch Laboratories (West Grove, PA), and Alexa Fluor 350-phalloidin was from Invitrogen/Molecular Probes (Carlsbad, CA). Aminooxypentane (AOP)-RANTES was a gift from Oliver Hartley (Centre Medical Universitaire).

Virus stock. Wild-type HIV_MN and HIV_ADA strains made in H9 or Jurkat cells were CD45 depleted to eliminate microvesicles as described previously (69). Briefly, supernatants from stably infected H9 cells containing virus were incubated with anti-CD45-conjugated microbeads (Miltenyi Biotec), and the CD45⁺ fraction (microvesicles) was retained inside the magnetized column, allowing CD45^– flowthrough (virus) to be collected. Viral preparations were then pelleted at 50,000 x g for 1 h at 4°C and resuspended in TNE buffer. Envelope glycoprotein on the virion surface remained functional, as shown by virus infectivity assays (26).

Flow cytometry. A total of 5 x 10⁵ cells were resuspended in 100 µl of 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) and incubated with a 1:200 dilution of MAbs raised against hCD4 (phycoerythrin-conjugated RPA-T4 MAb), hCCR5, or hCXCR4 at 4°C for 30 min. For CCR5 and CXCR4 staining, cells were washed twice in 1% BSA, resuspended in 100 µl of ice-cold 1% BSA, and incubated with 1:400 dilution of fluorescein isothiocyanate-conjugated anti-mouse immunoglobulin G for 30 min at 4°C. Finally, the cells were washed, resuspended in 200 µl of ice-cold 1% BSA, and analyzed by using FACSCalibur (Becton Dickinson).

Single-cell calcium mobilization assay. A total of 2 x 10⁶ cells in 1 ml of RPMI (15% FBS) were loaded with 4 µM Fluo4-AM (Invitrogen Co., Carlsbad, CA). After 20 min of incubation at 37°C, cells were washed in PBS and additionally incubated for 10 min at 37°C in serum-free RPMI. Prior to microscopy, primary T cells were transferred to T dishes (Bioptech, Inc., Butler, PA) with Cell-TAK (BD Biosciences, Bedford, MA)-coated glass surface and allowed to attach for 15 min at 37°C in serum-free RPMI. Ca²⁺ measurements were performed on an Olympus IX70 DeltaVision API deconvolution microscope attached to a charge-coupled device camera in a temperature-controlled chamber at 37°C. The cells were imaged in real time at 488 nm. Random fields containing 15 to 20 cells were photographed every 10 s for 1 to 5 min prior to the addition of stimulus (chemokine, recombinant gp120, or the virus) and continued to be photographed for different lengths of time (indicated in the figure legend for each experiment). The nanostage of the DeltaVision microscope allowed simultaneous observation of more than one field. To detect background fluorescence changes, Fluo-4-loaded cells were observed every 10 s without stimulation for 8 min (CHO-745 cells) or 40 min (primary T cells).

For each experiment, the images were converted into a movie file that could be analyzed for fluorescence intensity in distinctive regions of interest using ImageJ software (http://rsbweb.nih.gov/ij/docs/index.html). Ratios of green fluorescence at any time point (F) and initial fluorescence (F₀) are directly proportional to the Ca²⁺ concentration changes (52). Ratios were plotted over time for regions of interest representing individual cells to obtain a graph showing relative changes in Ca²⁺ concentrations. Using this approach, we were able to detect the pattern of Ca²⁺ fluxing in individual cells, including the amplitude, frequency, and kinetics.

Virus quantification. After Ca²⁺ fluxing experiments, cells were fixed directly in T dishes while on the microscope with 3.7% formaldehyde in PIPES buffer (100 nM PIPES [pH 6.8], 2 mM MgCl₂, 1 mM EGTA) for 5 min. Cells were washed with PBS and blocked for 5 min with the blocking solution (0.1% Triton X-100, 1% BSA, and 0.01% sodium azide in 10% normal donkey serum). Anti-HIV p24 MAb was added at a 1:250 dilution to cells in blocking solution, followed by incubation for 30 min at room temperature. Cells were then washed and incubated with a 1:400 dilution of anti-mouse secondary Cy5 antibody in blocking solution for 20 min at room temperature. After a wash with PBS, cells were incubated with 1:250 Alexa Fluor 350-phalloidin for 20 min in PBS. After the final wash, the cells were imaged in three dimensions by using deconvolution microscopy. Eighteen individual image slices were taken as part of the z-series, from the top to the bottom of the cells, in order to create a volume projection. Image fluorescence intensities were normalized to the background of the control cells not infected with the virus but stained in the same manner.

Statistical analysis. P values were calculated by using a two-tailed Student t test. Error bars represent the standard errors of the mean (SEM) unless otherwise noted. Statistical analysis was performed by using Microsoft Excel (Microsoft, Redmond, WA) and GraphPad software.

Supplemental material. Figure S1 in the supplemental material represents a time-lapse view of Fluo-4-loaded primary CD4⁺ T cells upon treatment with 10 ng of p24 HIV_MN/ml. Cells were imaged every 10 s for 40 min, and virus was added after the first 5 min (30 frames). Quantification of the data shown in Table 3 is presented. Figure S2 in the supplemental material displays the Ca²⁺ response in CHO-745 CD4⁺ CCR5⁺ cells to the R5-tropic HIV_ADA strain, indicating the functionality of HIV_ADA Env.

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TABLE 3. Virion-mediated Ca2+ fluxing in CD4+ T cells

Top Abstract Introduction Materials and Methods Results Discussion References

 
Monomeric gp120 induces coreceptor-specific Ca²⁺ mobilization. To detect HIV Env-initiated signaling cascades upon receptor engagement, we decided to monitor the changes in the secondary messenger, intracellular free Ca²⁺. To this end, Ca²⁺ concentration fluctuations within individual cells were visualized by real-time microscopy. For Ca²⁺ detection, cells were loaded with fluorescent Ca²⁺ indicator Fluo-4. The fluorescence intensity of Fluo-4 increases rapidly after it binds to free Ca²⁺ ions (52, 70) and is therefore directly proportional to the free Ca²⁺ concentration changes. Prior to all of the signaling experiments, we determined the baseline calcium fluctuations to distinguish the background changes from the actual Ca²⁺ flux. In this case, we imaged individual CHO-745 cells in the absence of any stimulation for 8 min. The free Ca²⁺ concentration in these cells fluctuated within a 1.5-fold range in the absence of stimuli and was used as a threshold value (Fig. 1A). Any signal greater than this threshold was scored as positive.

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FIG. 1. Specificity of gp120-mediated Ca2+ fluxing in CHO-745 cell lines. (A) Untreated CHO-745 CD4+ CCR5+ cells imaged for 500 s under the microscope to obtain the background Ca2+ fluctuation threshold, as described in Materials and Methods. The fluxing threshold was set to 1.5-fold, as indicated on the graphs. In all experiments, lines represent individual cells and are representatives of n, the total number of cells tested from a minimum of two experiments. The percentage value refers to the relative number of the cells fluxed ± the SEM. (B to F) CHO745 cell lines as indicated on the graphs treated with the indicated stimulus at the time point marked by an arrow. (G) Time-lapse images of CHO-745 CD4+ CXCR4+ cells from graph C. Scale bar, 17 µm. The numbers of the cells corresponding to the fluxing in response to SDF1 (panel C) are shown in the last panel.

To initiate this analysis, we chose the three previously characterized CHO-745 cell lines expressing human CD4⁺, CD4⁺ CCR5⁺, or CD4⁺ CXCR4⁺. These cell lines expressing different combinations of receptor and coreceptor served as an analytical tool, allowing us to compare the requirements for Env-mediated versus chemokine-mediated signaling. The CHO-745 cell lines showed the anticipated response when exposed to the appropriate chemokine. The specific ligand for CXCR4 is SDF1, and one of the ligands specific to CCR5 is chemokine regulated on activation, normal T-cell expressed and secreted (RANTES). CHO-745 CD4⁺ CCR5⁺ cells responded rapidly to the addition of 30 nM AOP-RANTES (Fig. 1B), and CD4⁺ CXCR4⁺ cells responded rapidly to 30 nM SDF1 (Fig. 1C). CHO-745 CD4⁺ cells did not respond to either AOP-RANTES or SDF1, as expected. In addition, no signaling was detected in CD4⁺ coreceptor expressing CHO-745 cells if treated with the nonmatching chemokine (data not shown). Ca²⁺ fluxing in response to the chemokines was heterogeneous overall. Rapid initial Ca²⁺ fluxing was observed as soon as 10 s in some cells, whereas the response was delayed in others. Often the same cell fluxed more than once in transient peaks (Fig. 1B and C), whereas some cells never responded during the period of analysis.

After establishing the pattern of CHO cell signaling with chemokines, we tested their responsiveness to recombinant monomeric gp120. We exposed three CHO-745 cell lines to R5-tropic gp120_BaL or X4-tropic gp120_HXB. By combining different coreceptors with gp120 of two different tropisms, we could get an insight into the specificity of the observed cellular response and test the importance of CD4 alone in signaling initiation.

We found that CHO-745 cells responded to gp120 after 1 to 2 min, which was delayed compared to the chemokine-induced signal. CHO-745 CD4⁺ CCR5⁺ but not CD4⁺ CXCR4⁺ cells responded to 30 nM R5-tropic gp120_BaL (Fig. 1E). CHO-745 CD4⁺ CXCR4⁺ cells mobilized Ca²⁺ in response to the matching X4-tropic gp120_HXB (Fig. 1F) but required a higher gp120 concentration. No response was observed until a 2 µM concentration was used. At the same time, single positive CHO-745 CD4⁺ cells did not generate Ca²⁺ signal after treatment with as much as 2 µM gp120_HXB (Fig. 1D). Altogether, these results implied that the Ca²⁺ signaling we were detecting upon gp120 addition was coreceptor specific and solely coreceptor mediated. However, it is worth noting that the effective stimulatory gp120 concentration in CHO cell lines is superphysiological considering the potential in vivo gp120 levels (38).

Gp120-mediated Ca²⁺ fluxing in primary unstimulated CD4⁺ T cells is coreceptor mediated. To test the coreceptor specificity in a more physiologically relevant environment, we focused on the requirements for gp120-mediated signaling ex vivo in primary unstimulated CD4⁺ T cells. This heterogeneous cell population expresses CXCR4 (12, 51) but not CCR5, therefore representing an ideal system for examining coreceptor specificity in gp120-mediated signaling.

Again, the background Ca²⁺ fluctuation was determined by monitoring untreated CD4⁺ T cells for 40 min, which maintained Ca²⁺ levels within a twofold range (Fig. 2A). Only 2.5% ± 0.5% cells elevated the Ca²⁺ level above this threshold in the absence of stimuli. SDF1 treatment caused a rapid Ca²⁺ fluxing in 34.5% ± 1.8% cells (Fig. 2B and Table 1) . The response in these cells was consistent with results obtained from the CHO-745 CD4⁺ CXCR4⁺ cell line (compare Fig. 1C and Fig. 2B). The first T cells responded within 10 s, often in more than one transient peak, where the calcium level stayed elevated for an average of 1.1 ± 0.1 min per peak. Higher SDF1 concentrations (up to 500 nM) increased the number of Ca²⁺ fluxes per cell but could not increase either the relative number of responsive cells or the amplitude of the response (data not shown).

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FIG. 2. Specificity of gp120-mediated Ca2+ fluxing in primary unstimulated CD4+ T cells. (A) Primary CD4+ T cells imaged for 40 min without any stimulation to obtain background Ca2+ fluctuations. This twofold threshold is indicated on all graphs. Each line represents an individual cell. (B to D) CD4+ T cells treated with 30 nM SDF1 (B), 100 nM gp120HXB (C), or 20 nM gp120HXB (D) at the time points indicated by the arrows. (E) Cells treated with 100 nM gp120BaL (thick arrow) and subsequently with 100 nM gp120HXB (thin arrow). All figures are representative of a minimum of three experiments. A quantification of these data is also shown in Table 1.

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TABLE 1. Monomeric gp120-mediated Ca2+ fluxing in CD4+ T cells

We next sought to determine whether and under which conditions unstimulated primary CD4⁺ T cells respond to recombinant monomeric X4-tropic gp120_HXB. Indeed, T cells did mobilize intracellular Ca²⁺ after gp120_HXB treatment (Table 1). The calcium level remained elevated for a maximum 14 min per peak, with an average of 4.6 ± 0.8 min per peak, which was significantly longer than the peak caused by SDF1 (P < 0.01). At 100 nM gp120_HXB, there was a 2- to 3-min lag phase before any flux was detected (Fig. 2C). This lag phase was detected even at higher gp120 concentrations and was prolonged when lower gp120_HXB concentrations were used. For example, with 20 nM gp120_HXB, the lag phase ranged from 5 to 7 min (Fig. 2D), and a significantly lower percentage of cells responded (Table 1). At less than 20 nM gp120_HXB we could not detect changes in intracellular Ca²⁺ concentrations above the twofold threshold within 15 min of addition. Therefore, the unstimulated CD4⁺ T cells were 100-fold more sensitive in their response to X4-tropic gp120 than the CHO-745 CD4⁺ CXCR4⁺ cells described above. Blocking CXCR4 signaling ability with a small inhibitory molecule, AMD3100 (63), allowed us to confirm the coreceptor specificity of the observed Ca²⁺ mobilization. The values shown in Table 1 indicate that 10 µM AMD3100 significantly abrogated gp120_HXB-mediated cell signaling initiation (P < 0.001). Moreover, 100 nM R5-tropic gp120_BaL could not cause Ca²⁺ fluxing in tested CD4⁺ T cells (Table 1), where the CCR5 surface expression was <1.2% (data not shown). Thus, the observed signaling was mediated through the cognate coreceptor.

CD4 is necessary for gp120-mediated Ca²⁺ fluxing in primary unstimulated CD4⁺ T cells. The results described above questioned the role of CD4 in gp120-mediated Ca²⁺ signaling, since the lack of response to R5-tropic gp120_BaL was observed in primary CD4⁺ T cells (Table 1) and to gp120_HXB in single positive CHO-745 CD4⁺ cells (Fig. 1D)_. These data suggested that CD4 binding alone was insufficient for Ca²⁺ mobilization.

Interestingly, R5-tropic and X4-tropic gp120 appeared to compete for CD4 binding. The pretreatment of primary CD4⁺ T cells with 100 nM R5-tropic gp120_BaL abolished signaling by 100 nM X4-tropic gp120_HXB (Fig. 2E). This suggested a role for CD4-gp120 binding in Ca²⁺ signaling, even though the signal was mediated through CXCR4. gp120 probably required CD4 binding prior to CXCR4 engagement, a view consistent with the previous results showing that CD4 engagement was required for V3 loop exposure in gp120 and a subsequent interaction with coreceptor (24, 41, 72).

To test CXCR4 signaling in a CD4-free environment, we used primary CD8⁺ CD4^– T cells that express CXCR4 at comparable levels to primary CD4⁺ T cells (Fig. 3A). CD8⁺ T cells were responsive to SDF1 in a fashion similar to CD4⁺ T cells. Most of the cells responded rapidly, in transient peaks (Fig. 3B and Table 2). CD8⁺ T cells were, however, unable to elevate Ca²⁺ upon addition of up to 2 µM gp120_HXB, even though the same cells responded to SDF1 (Fig. 3C). Nevertheless, when we provided CD4 in trans by adding soluble CD4 (sCD4) to the cells prior to gp120_HXB addition, CD8⁺ T cells elevated Ca²⁺ after a 1-min lag (Fig. 4B).

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FIG. 3. CD8+ T-cell response to monomeric gp120. (A) Flow cytometry of primary unstimulated CD4+ and CD8+ T cells 24 h after isolation from peripheral blood. Plots show the expression levels of CD4 or CXCR4 in CD4+ T cells (thin line) and CD8+ T cells (thick line), with isotype control antibodies (gray histogram). (B and C) CD8+ T cells treated with 30 nM SDF1 (B) or 200 nM gp120HXB (thick arrow) and subsequently with 30 nM SDF1 (thin arrow) (C). (D) 200 nM gp120HXB preincubated with 500 nM sCD4 for 5 min at 37°C prior to addition onto CD8+ T cells. All figures are representative of a minimum of two experiments. Each line represents an individual cell. A quantification of these data is also shown in Table 2.

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TABLE 2. Selective effect of sCD4 on gp120-mediated Ca2+ fluxing in CD4+ and CD8+ T cells

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FIG. 4. sCD4 altered fluxing kinetics in T cells. (A) Relative numbers of fluxing CD4+ or CD8+ T cells (n) plotted over the time it took them to elevate Ca2+ above the twofold threshold upon the addition of 30 nM SDF1. (B) Fluxing kinetics of CD8+ T cells in response to 200 nM sCD4, subsequently treated with 200 nM gp120HXB (1:1 molar ratio). (C) Fluxing kinetics of CD8+ T cells treated with preincubated sCD4-gp120HXB complex in 5:2 molar ratio. (D to F) Fluxing kinetics of CD4+ T cells in response to 200 nM gp120HXB (D), preincubated sCD4-gp120HXB complex in a 1:2 molar ratio (E), or preincubated sCD4-gp120HXB complex in a 1:1 molar ratio (F). All graphs show the average values ± the standard deviation obtained only from fluxing cells (n). Quantitative data from these experiments are also shown in Table 2.

In contrast, no lag phase in Ca²⁺ mobilization was detected when gp120_HXB and sCD4 were preincubated for 5 min at 37°C and then added to CD8⁺ T cells (Fig. 3D and 4C). Thus, prebinding of gp120 to sCD4 appeared to remove a kinetic step allowing signaling to occur on the same timescale as that of chemokine SDF1 (Fig. 3D). The observation that the prebinding of gp120 and sCD4 removes the lag before signaling suggests that the observed lag was not due to the slower diffusion rate of gp120 compared to SDF1. It rather reflects the time for gp120 to bind CD4, change its conformation to expose the V3 loop, engage the coreceptor, and subsequently initiate Ca²⁺ flux. It is worth noting that sCD4 did not alter normal SDF1-mediated activation of CXCR4 signaling (Table 2).

In contrast to CD8⁺ T cells, treatment of CD4⁺ T cells with gp120_HXB preincubated with sCD4 failed to accelerate signaling initiation. Actually, sCD4 had an inhibitory effect on the kinetics of Ca²⁺ signaling. As shown in Fig. 4E, with a 1:2 molar ratio of sCD4 to gp120, the lag phase increased from 2 to 3 min up to 3 to 6 min, and with 1:1 molar ratio it increased up to 5 to 8 min. Increasing concentrations of sCD4 prolonged the lag phase and decreased the number of the responding cells until the total abrogation of the signal (Table 2). Therefore, the soluble gp120-sCD4 complex behaved differently in the presence of transmembrane CD4 on the target cells.

Low levels of X4-tropic virions mediate Ca²⁺ fluxing in primary unstimulated CD4⁺ T cells. The results presented above reveal that CD4⁺ T cells respond to monomeric X4-tropic gp120 and that the generated Ca²⁺ signal is CXCR4-mediated but dependent on CD4 binding. However, in its native form, virion-bound Env is a trimer composed of three gp120 exterior glycoproteins and three transmembrane gp41 glycoproteins (22, 74). Therefore, the ability of virion-bound, trimeric, X4-tropic gp120_MN to activate CXCR4-mediated Ca²⁺ fluxing in CD4⁺ T cells was determined. In all of the following experiments, T cells were exposed to replication-competent HIV_MN virions depleted of CD45 (69). This viral preparation contained a highly active homogeneous stock, wherein general debris and microvesicles have been removed to avoid nonspecific artifacts in signaling (14).

The response of CD4⁺ T cells to HIV_MN was dose dependent. The number of cells that elevated the Ca²⁺ above the twofold threshold within the first 35 min positively correlated with the HIV_MN p24 concentration (Table 3). The lowest p24 concentration that stimulated fluxing was 10 ng of p24 HIV_MN/ml (Fig. 5A and Fig. S1 in the supplemental material), which led to fluxing in 17.4% ± 0.2% cells.

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FIG. 5. Virion-mediated Ca2+ fluxing in CD4+ T cells. (A) CD4+ T cells treated with 10 ng of p24 HIVMN/ml at the time point indicated by an arrow. The graph represents data obtained from the signaling movie shown in the supplemental material. (B) Cells preincubated with 10 µM AMD3100 for 25 min treated with 10 ng of p24 HIVMN/ml. (C) CD4+ T cells treated with 10 ng of p24 HIVADA/ml. (D) Kinetics of CD4+ T cell response to HIVMN. Shown are the relative numbers of 80 cells that fluxed upon the addition of 10 ng of p24 HIVMN/ml, distributed by the time necessary to elevate the Ca2+ level above twofold threshold. The values are averages of 26 experiments ± the SEM. Dotted lines (2.5% ± 0.5%) represent the cutoff for cells that nonspecifically fluxed in threshold determination experiments (Table 1). Quantitative data from all graphs are also shown in Table 3.

Coreceptor specificity in virion-mediated fluxing is shown in Fig. 5B, wherein the pretreatment of primary CD4⁺ T cells with CXCR4 inhibitor AMD3100 diminished Ca²⁺ signaling. While 10 ng of p24 HIV_MN/ml activated Ca²⁺ fluxing in T cells, the same concentration of R5-tropic HIV_ADA failed to induce any signals over background levels (Fig. 5C). The results obtained from CHO-745 cells treated with virions also implied coreceptor specificity in fluxing: 10 ng of p24 HIV_ADA/ml elevated Ca²⁺ in 20.4 ± 1.8% CHO-745 CD4⁺ CCR5⁺ cells (Fig. S2 in the supplemental material), and yet HIV_MN did not activate fluxing in a single CHO-745 CD4⁺ CCR5⁺ cell of 22 tested (data not shown). Thus, virion-mediated signaling requires an Env-coreceptor match, reflecting the results obtained with monomeric gp120.

The pattern of HIV_MN-induced Ca²⁺ elevation resembled that observed for monomeric gp120_HXB. Coreceptor response to 10 ng of p24 HIV_MN/ml was at least three minutes delayed (Fig. 5A and D), whereas some cells took longer than 3 min to activate signaling (Fig. 5D). The average length of Ca²⁺ peaks was 3.72 ± 0.9 min and was not statistically different from the length of monomeric gp120_HXB-mediated Ca²⁺ flux. The high heterogeneity in kinetics and pattern of cellular response to the virus is likely due to the kinetics and frequency of viral attachment events to target cells.

To gain insight into how virion binding correlates to the Ca²⁺ fluxing pattern, the number of viral particles bound to each T cell were counted. After the fluxing experiments, the cells were fixed and stained for HIV p24 capsid protein. The fluorescent p24 signal was normalized to the control virus-free cells stained for p24, and each round dot above the background pixel threshold was counted as a single virion (Fig. 6). In all cases, virions were randomly dispersed on the surfaces of target cells (Fig. 6B). It appears that Ca²⁺ had been mobilized only in a subset of p24⁺ cells (Fig. 7A), as observed in the T-cell responses to SDF1 and monomeric gp120 (Table 1). Importantly, we found that 99% ± 0.7% of cells that did flux had detectable p24 signal on their plasma membranes. The majority of p24⁺ cells did not flux (Fig. 7A), but the portion of p24⁺ fluxing cells increased over time (Fig. 7A). For instance, between 10 and 35 min the percentage of fluxing p24⁺ cells increased from 5.3% ± 0.6% to 17.4% ± 0.2% in cells treated with 10 ng of p24/ml and from 4.1% ± 0.6% to 27.5% ± 0.7% in cells treated with 100 ng of p24/ml (Fig. 7A and Table 3). Among individual experiments, a positive correlation between the frequency of Ca²⁺ fluxing and the average virion number per cell was noticed 10 min after the HIV_MN treatment. This correlation was somewhat decreased by 35 min (Fig. 7B), possibly due to responses to previously bound virus or the ongoing release or turnover of virus on cell surface.

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FIG. 6. Binding of HIVMN virions to individual primary CD4+ T cells. (A) Example of virion-binding experiment. Fluo-4-loaded cells were observed under a microscope (x100) every 10 s for 40 min and treated with 10 ng of p24 HIVMN/ml after the first 300 s. Scale bar, 7 µm. (B) After 40 min of the signaling experiment, the cells were fixed and stained for HIV-1 p24 (red) and actin (blue). Eighteen images from the top to the bottom of the cells were digitally overlapped to give one image, a volume projection of the z-series. Therefore, all of the virions were found on the cell surface. (C) Red p24 signals were counted, and the numbers correlated to the pattern of Ca2+ fluxing in the same cells. Relative Ca2+ concentration changes were plotted over time.

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FIG. 7. Virion distribution on fluxing versus nonfluxing T cells. (A) Percentage of cells that had the virus (p24 signal) on their surface 2, 10, or 35 min upon addition of 10 or 100 ng of p24 HIVMN/ml. The upper portions of the bars represent the percentage of p24-positive cells that also had fluxed. Values were averaged from a minimum of four experiments ± the SEM. (B) Positive correlation between the percentage of cells fluxed and average number of virions per cell in individual experiments. Each dot represents a single experiment. The plots show the correlation 10 or 35 min upon the addition of 10 ng of p24 HIVMN/ml. r2, coefficient of determination. (C) Data show the average virion numbers found on cells that fluxed versus cells that did not 2, 10, or 35 min after treatment with 10 or 100 ng of p24 HIVMN/ml. Shown are the average values from nine experiments ± the SEM. ND, not determined. *, Data also represented in graph B. (D) Distribution of virion numbers attached to individual cells that fluxed versus cells that did not flux in response to 10 ng of HIVMN/ml during a 35-min interval. The average of nine experiments ± the SEM is given.

Figure 7C represents the quantification of HIV p24 signal on cells that fluxed versus ones that did not flux upon different HIV_MN treatments. A time- and concentration-dependent increase in average virion numbers on cells that did not flux was observed. In contrast, cells that did flux had an average of four virions attached, even under different experimental conditions (Fig. 7C). A statistical difference in the number of virions bound to fluxing versus nonfluxing T cells can be seen 10 min after viral addition. This difference is lost 35 min into the treatment, since now more viruses landed on nonfluxing cells. Interestingly, even though there was no difference in the average virion numbers bound to the two cell groups after 35 min (Fig. 7C), the distribution of virions on responding and nonresponding cells is certainly different (Fig. 7D). We detect 1 to 12 virions bound to an individual responsive cell. As mentioned above, these cells were always found with at least one viral particle, but most often with two or three. However, cells that did not elevate Ca²⁺ after the same HIV_MN treatment were often found with no virus, and no obvious preference for the distribution of virion particles among cells was observed.

Top Abstract Introduction Materials and Methods Results Discussion References

 
How CD4⁺ T cells respond to binding of HIV envelope glycoprotein is a controversial issue. We sought to determine whether low numbers of free monomeric (gp120_m) and virion-associated trimeric gp120 (Env) could be recognized by CD4⁺ T cells through signal transduction pathways initiated by signaling molecules CD4 and CXCR4, which serve as receptors in X4-tropic HIV entry (25, 32, 48, 57, 65). As a marker for the initiation of signaling, we chose to monitor the oscillations of a secondary messenger, free intracellular Ca²⁺. Detecting Ca²⁺ changes by real-time microscopy allowed the kinetics and requirements for activation of Env-mediated signaling to be studied in individual primary unstimulated CD4⁺ T cells. This approach enabled the sensitive detection of signaling at extremely low concentrations of X4-tropic Env.

Our data showed that both gp120_m and Env were able to induce signaling in cells expressing CD4 and a coreceptor. gp120_m- and Env-mediated coreceptor-specific Ca²⁺ fluxing was only observed when the matching coreceptor responded to R5-tropic or X4-tropic gp120. The engagement of CD4 alone could not mobilize Ca²⁺ within our detection range, which suggested that Ca²⁺ signal was being mediated solely through the coreceptor. This was confirmed because signaling mediated by X4-tropic gp120 was sensitive to CXCR4 inhibitor AMD3100. In addition, we showed that coreceptor-mediated Ca²⁺ fluxing upon HIV gp120 binding was CD4 dependent. Primary CD8⁺ CD4^– CXCR4⁺ T cells were observed to elevate Ca²⁺ in response to gp120_m only in the presence of soluble CD4. This was in agreement with previous reports that gp120 activates the coreceptor and leads to Ca²⁺ fluxing and mitogen-activated protein kinase pathway signaling only upon previous gp120-CD4 interaction (56, 71). However, even though CD4 was required, it did not have to be on the cells surface, strengthening the conclusion that signaling was coreceptor mediated.

The pattern of Ca²⁺ mobilization initiated by both gp120_m and Env was qualitatively different from that induced by chemokines. Chemokines AOP-RANTES and SDF1 caused rapid, transient Ca²⁺ peaks through CCR5 or CXCR4, respectively. In contrast, a delayed, sustained plateau of Ca²⁺ was a consequence of gp120_m and Env treatments. This phenomenon was independent of gp120 tropism and concentration. Also, when endogenous CD4 was absent, as in primary CD8⁺ T cells, a gp120-sCD4 complex mobilized Ca²⁺ through CXCR4 in a similar fashion as SDF1 did, with multiple transient peaks. This finding suggested that the signal generated by a cell-associated gp120-CD4-CXCR4 complex was qualitatively different from the chemokine signaling. That is, when CD4 was present in the membrane of target cells along with the matching coreceptor, gp120 elicited a unique extended pattern of Ca²⁺ elevation. This is reasonable given the fact that CD4 is a signaling molecule that is capable of transmitting signals upon gp120 binding (65). Alternatively, the simultaneous engagement of CD4 and coreceptor may prevent the normal endocytosis of the chemokine receptor. Endocytosis and subsequent acidification of a GPCR causes the ligand to be released ending the signaling. Engaging CD4 and chemokine receptor simultaneously may alter this process, leading to a sustained signal. This may be especially important in the context of virion-associated envelopes where multiple molecules are likely simultaneously engaged. The importance of this unique signaling pattern might be related to recent findings that T and B cells are able to differentiate the quality of outside stimuli even at the level of a secondary messenger. Different Ca²⁺ elevation patterns, including the amplitude, frequency, and the length of elevation can lead to the activation of different transcription factors and therefore to distinct cellular responses (13, 15, 18, 19, 45, 67, 73). However, since we did not test any of the downstream effectors of CD4 and GPCR signaling, CD4-CXCR4 synergy in gp120-mediated signaling requires future investigation.

The presence of a lag phase in coreceptor activation was an additional difference between the effects of chemokine and gp120 glycoprotein. Unlike the rapid SDF1-mediated calcium mobilization, there was a statistically significant delay in CXCR4 activation by both gp120_m and Env. The earliest CXCR4 activation events occurred 2 to 3 min after the gp120 treatment, a finding consistent with other published data on the existence of a lag phase in HIV-1 fusion (17, 20, 36, 50, 54, 59). A likely explanation is that the lag interval represents the time necessary for gp120 attachment to CD4, conformational changes to expose the V3 loop, and a subsequent coreceptor engagement (24, 33, 41, 53, 61, 72). Consistent with that, no delay was detected in CD8⁺ T cells response to preincubated complex of gp120_m and sCD4, suggesting that coreceptor engagement and signaling are relatively fast, similar to the SDF1 effect. Our assumption is in agreement with the work of LaBranche et al. (40) and Gallo et al. (26), who showed a reduced time lag in the fusion of CD4-independent gp120 mutant compared to the wild type. In addition, data obtained from prior temperature-arrested fusion state studies indicate that CD4 engagement is the first rate-limiting step in viral fusion (30, 49).

It is worth noting that a 3-min lag in coreceptor activation with gp120_m was present at concentrations above and around its K_d for CXCR4 binding (200 nM) (9). Nevertheless, a 10-fold-lower gp120_m concentration caused slower coreceptor engagement kinetics in a reduced number of CD4⁺ T cells. A similar effect was seen as a result of soluble CD4 on gp120-mediated signaling in CD4⁺ T cells where sCD4 acted in an inhibitory manner on the signaling kinetics. This would be analogous to the observations of Wulfing et al. (73) that the loss of the ligand strength is reflected in the prolonged initiation of calcium elevation. The inhibitory effect of sCD4 on CD4⁺ T cells kinetics could argue that some kind of interference of transmembrane CD4 on CXCR4 activation may be a consequence of spatial restriction, since the receptor and coreceptors are found in close proximity in the cell membrane (39, 66, 75, 76). More specifically, CD4 as a signaling molecule could send a prerequisite signal that is insufficient for causing Ca²⁺ flux alone (39) but somehow modifies signaling through CXCR4 upon gp120-CXCR4 binding. This model is consistent with the qualitative differences in Ca²⁺ flux mediated by soluble CD4- and gp120-treated CD4⁺ T cells versus membrane-bound gp120-CD4 complexes, as previously mentioned. Therefore, a possible competition of two CD4 forms for gp120 could have either impaired the synergy of CD4-CXCR4 signaling or lowered the concentration of properly folded gp120 needed to engage the coreceptor in a functional gp120-CD4-CXCR4 complex.

Heterogeneity in response among individual cells was seen repeatedly. This argues for the existence of a subpopulation in an isolated primary CD4⁺ T-cell fraction that is sensitive to X4-tropic gp120, whereas a majority of cells never fluxed, even at high concentrations of stimuli. This differential response by primary unstimulated CD4⁺ T cells might be due to the disparate coreceptor expression levels on cells, the expression of different CXCR4 isoforms (42, 44, 64), or the presence of a quiescent T-cell population, or it might be a virus-induced response in only a certain type of peripheral blood CD4⁺ CXCR4⁺ T cells, such as naive, regulatory, or memory cells. In all of our experiments, up to 35% cells responded to the given stimuli, including chemokines, gp120_m, or the virus, never reaching a 100% response. Since this observation has been previously reported (7, 60), it calls for further investigation.

We have found that the lowest effective concentration of monomeric X4-tropic gp120 was in the lower nanomolar range, and its in vivo relevance is still debatable (38). On the other hand, virion-bound gp120 was one thousand times more potent in inducing signaling in unstimulated primary CD4⁺ T cells. The lowest effective viral concentration was 10 ng of p24/ml, which corresponds to 0.42 nM p24 (23), 10⁸ virions (43), 8 pM gp120, or 8.3 pg gp120 (23), which is significantly higher than the highest levels of HIV found in infected patients during acute infection (10⁶ to 10⁷ RNA copies/ml of plasma) (62). Therefore, even 10 ng of p24/ml appears to be superphysiological. However, we have found that two or three HIV particles or ca. 28 to 42 gp120 trimers (77) per cell was sufficient to activate Ca²⁺ signaling through CXCR4. In some cases, even one viral particle caused the signaling in T cells. Signal induced by a single virion is not improbable, since Irvine et al. (34) found that a single peptide ligand could activate TCR signaling in T cells and Baylor et al. (11) showed that retinal cells of the eye could detect single photons, indicating a maximum sensitivity for these signal transduction systems to minimal respective stimuli. The probability that two or three virions bind a CD4⁺ T-cell in vivo is very likely based on the range of plasma HIV RNA copies and CD4⁺ T-cell counts in chronically infected patients (21, 46, 68). Importantly, this signaling takes place in the absence of infection, since resting CD4⁺ T cells are refractory to infection. This indicates that virion-mediated signaling is likely to be happening at high frequency in infected individuals. In light of numerous reports that gp120-mediated signaling can have deleterious effects on cell function and even induce apoptosis (2, 6, 16), it is possible that such X4-tropic virion signaling may play a role in bystander effects in infected patients, contributing to the immunodeficiency without an active infection.

Based on our findings, we consider measurements of intracellular Ca²⁺ changes to be a sensitive and direct way to study the kinetics of coreceptor engagement during HIV entry. A real-time microscopy-based ex vivo system reveals responses in live natural target cells to wild-type virus at physiologically relevant concentrations and therefore involves the parameters necessary to establish a case of physiological significance. Using this system, we were able to detect CXCR4 signaling activation after the binding of two viral particles. The ability of a few virions to stimulate signaling in target cells without an active infection could have important implications in AIDS pathogenesis.

 
We thank Robert W. Doms and the Neutralizing Antibody Consortium funded by the IAVI for the recombinant gp120_HXB, Paul Bieniasz for the CHO-745/CycT cell lines, and Oliver Hartley for the AOP-RANTES. We also thank Julian Bess for purifying the viral preparations, Robert Stahelin for recommending Fluo-4, and Suchita Bhattacharyya and Edward Campbell for help with the fluorescence-activated cell sorting analysis.

This study has been funded through the National Institutes of Health, grant RO1 AI52051 (to T.J.H.), and funded in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. T.J.H. is an Elizabeth Glaser scientist.

The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

 
* Corresponding author. Mailing address: Northwestern University, Department of Cell and Molecular Biology, Feinberg School of Medicine, Ward 8-140, 303 E. Chicago Ave., Chicago, IL 60611. Phone: (312) 503-1360. Fax: (312) 503-2696. E-mail: thope{at}northwestern.edu.

Published ahead of print on 22 November 2006.

Supplemental material for this article may be found at http://jvi.asm.org/.

Top Abstract Introduction Materials and Methods Results Discussion References

 

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Journal of Virology, February 2007, p. 1773-1785, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.01316-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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