Skip to main page content

• HOME
• Current Issue
• Archive
• Alerts
• About ASM
• Contact us
• Tech Support
• Journals.ASM.org

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, M. Articles by Banerjee, A. K. Search for Related Content PubMed PubMed Citation Articles by Chen, M. Articles by Banerjee, A. K.

 Previous Article  |  Next Article 

Journal of Virology, December 2007, p. 13478-13485, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01244-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Interaction of Vesicular Stomatitis Virus P and N Proteins: Identification of Two Overlapping Domains at the N Terminus of P That Are Involved in N⁰-P Complex Formation and Encapsidation of Viral Genome RNA

Mingzhou Chen, Tomoaki Ogino, and Amiya K. Banerjee^*

Department of Molecular Genetics, Section of Virology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195

Received 7 June 2007/ Accepted 21 September 2007

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
The nucleocapsid (N) protein of nonsegmented negative-strand (NNS) RNA viruses, when expressed in eukaryotic cells, aggregates and forms nucleocapsid-like complexes with cellular RNAs. The phosphoprotein (P) has been shown to prevent such aggregation by forming a soluble complex with the N protein free from cellular RNAs (designated N⁰). The N⁰-P complex presumably mediates specific encapsidation of the viral genome RNA. The precise mechanism by which the P protein carries out this function remains unclear. Here, by using a series of deleted and truncated mutant forms of the P protein of vesicular stomatitis virus (VSV), Indiana serotype, we present evidence that the N-terminal 11 to 30 amino acids (aa) of the P protein are essential in keeping the N protein soluble. Furthermore, glutathione S-transferase fused to the N-terminal 40 aa by itself is able to form the N⁰-P complex. Interestingly, the N-terminal 40-aa stretch failed to interact with the viral genome N-RNA template whereas the C-terminal 72 aa of the P protein interacted specifically with the latter. With an in vivo VSV minigenome transcription system, we further show that a deletion mutant form of P (P1-10) lacking the N-terminal 10 aa which is capable of forming the N⁰-P complex was unable to support VSV minigenome transcription, although it efficiently supported transcription in vitro in a transcription-reconstitution reaction when used as purified protein. However, the same mutant protein complemented minigenome transcription when expressed together with a transcription-defective P deletion mutant protein containing N-terminal aa 1 to 210 (PII+III). Since the minigenome RNA needs to be encapsidated before transcription ensues, it seems that the entire N-terminal 210 aa are required for efficient genome RNA encapsidation. Taking these results together, we conclude that the N-terminal 11 to 30 aa are required for N⁰-P complex formation but the N-terminal 210 aa are required for genome RNA encapsidation.

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
Vesicular stomatitis virus (VSV) is a negative-strand RNA virus belonging to the family Rhabdoviridae. Its genome RNA is enwrapped in a helical form with the nucleocapsid (N) protein and packaged within the virion together with the RNA-dependent RNA polymerase large protein (L) and the phosphoprotein (P). During cell infection, the associated RNA polymerase transcribes the genome RNA into five mRNAs that are subsequently translated into the glycoprotein (G), the matrix (M) protein, and the N, P, and L proteins. The N protein is an RNA-binding protein since it forms a high-molecular-weight complex with cellular RNAs with a quasihelical structure (1, 16) when expressed from plasmids in bacterial, mammalian, or insect cells. In contrast, it specifically encapsidates the viral genome RNA during the course of infection. It has been suggested that the P protein acts as a specific chaperone and forms a soluble complex with the N protein and prevents the latter from concentration-dependent aggregation (11, 19, 23, 25). The N-P complex, once formed, prevents the N protein from binding to cellular RNAs, keeps it in an encapsidation-competent form, and provides specificity to encapsidate the viral RNA during replication. This soluble complex, a vital component of replication containing RNA-free N protein, is designated N⁰-P (8, 11, 24, 31). Both in vitro and in vivo studies have also shown that P forms specific complexes with N⁰ with various stoichiometries (11, 22, 24). Thus, the P protein of VSV, and presumably of other NNS RNA viruses, serves three important functions in RNA synthesis. (i) It structurally stabilizes L protein (14, 17, 28, 34) to bind to the N-RNA template to form the active L-P₂ holoenzyme (13); for VSV, phosphorylation of P protein is essential to form this active holoenzyme (30). (ii) P protein forms a specific complex with N⁰ and prevents N protein from binding to cellular RNAs, which is an essential step for replication since newly formed N⁰-P complex initiates and encapsidates viral cRNA and newly synthesized genome RNA (14, 18, 19, 25, 31, 35). Interestingly, unlike formation of an active L-P₄ complex, phosphorylation of P protein is not required for N⁰-P complex formation (36). (iii) For VSV, the P protein forms a tripartite complex with the L and N proteins to form the replicase complex to transcribe the genome and antigenome RNAs end to end with concomitant encapsidation of RNA by the N protein elicited from the N⁰-P complex (32). Although it is known that the N and C termini of the P protein are involved in binding to N⁰ and N-RNA template, respectively (36), it is not known whether N⁰-P complex formation is necessary and sufficient for encapsidation of viral RNA. Here, we demonstrate that N⁰-P complex formation is necessary but a defined N-terminal region of the P protein directly regulates encapsidation of viral genome RNA.

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
Materials. The anti-Myc polyclonal antibody (Ab) (sc789), the monoclonal Ab (sc-40), and the anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal Ab (sc-32233) used were from Santa Cruz Biotechnology, Inc. The anti-glutathione S-transferase (GST) polyclonal Ab used and protein G-Sepharose 4 Fast Flow were from Amersham Biosciences. The anti-HA (clone HA-7) and anti-Flag monoclonal Abs used were from Sigma, and the anti-six-His monoclonal Ab (ab18184) used was from Abcam Inc. Anti-VSV N, P, and L polyclonal Abs were raised in rabbits and supplied by Invitrogen. ATP, GTP, UTP, and CTP were obtained from Roche Molecular Biochemical. [-³²P]GTP (3,000 Ci/mmol) was from Perkin-Elmer.

Cells and viruses. HeLa and BHK-21 cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. Recombinant vaccinia virus (vTF7-3) carrying the bacteriophage T7 RNA polymerase gene was propagated as described by Pattnaik et al. (30).

Plasmid constructs. HA-tagged wild-type P, P1-40, phosphorylation site mutant protein P3A (S60A, T62A, S64A), PHinge, P161-210, PII+III, and P76-137; Myc-tagged wild-type N and P; and Flag-tagged L, pBS-N, pBS-P, pBS-L, and pVSV-CAT2 have been described previously (7). cDNAs encoding other mutant P proteins were amplified with pET3a-P (2) as the template and cloned in frame with the open reading frame (ORF) encoding the GAL4 activating domain and a hemagglutinin (HA) tag into pGADT7 (Clontech). cDNA encoding P1-10 was also cloned in frame with the ORF encoding the GAL4 DNA binding domain and a Myc tag into pGBKT7 (Clontech). GST-fused P peptides were amplified with pGEX-6P3 and pET3a-P as templates, respectively. The resultant PCR products were mixed as templates, and reamplified cDNAs encoding GST-fused peptides were cloned in frame with the ORF encoding the GAL4 activating domain and an HA tag into pGADT7. All constructs were verified by DNA sequencing.

Detection of soluble N protein and in vivo coimmunoprecipitation. A plasmid encoding the N protein was transfected alone or cotransfected with plasmids encoding wild-type P, mutant P proteins, or GST-fused peptides with Lipofectamine 2000 (Invitrogen) into HeLa cells in six-well plates, followed by vTF7-3 infection. After 24 h of transfection, cell lysates were washed once with phosphate-buffered saline and lysed in lysis buffer (6). Lysates were centrifuged at 13,000 rpm for 30 min at 4°C to separate the supernatant and pellet. The soluble N proteins in supernatants in all subsequent studies and only aggregated N proteins in pellets were analyzed by Western blotting (WB) with anti-Myc monoclonal Ab. To detect interaction between the L and P proteins, Flag-tagged L and HA-tagged P protein or mutant P proteins were coexpressed. Supernatants from lysates were subjected to immunoprecipitation with anti-L polyclonal Ab as described above. Bound proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and detected by WB with monoclonal anti-Myc, anti-HA, or anti-Flag Abs.

Separation of N⁰-P and N-RNA complexes by ultracentrifugation. A 0.5-µg sample of a plasmid encoding Myc-tagged N was transfected alone or cotransfected with increasing amounts of plasmids (0.25 µg, 0.5 µg, 1.0 µg, and 2.0 µg) encoding HA-tagged GST-1-40 (PN40) or GST-192-265 (PC72), described above. Cell pellet was incubated in 2.4 ml of lysis buffer (20 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1.9% [wt/vol] Triton X-100, 1 mM 1,4-dithiothreitol, 5% [vol/vol] glycerol) for 30 min on ice. Lysates were centrifuged as described above, and supernatants were layered onto 1.6 ml 20% (vol/vol) glycerol in TE (20 mM Tris-HCl [pH 8.0], 1 mM EDTA) and ultracentrifuged further in a Sorvall TH-660 rotor at 35,000 rpm for 4 h at 4°C to separate the supernatant (U-sup) and pellet (U-ppt). The U-ppt was resuspended in 50 µl of TE containing 10% (vol/vol) glycerol. Both the U-sup and U-ppt were boiled in Laemmli buffer and detected by WB with monoclonal anti-Myc Ab to detect N protein.

N-RNA template purification and GST pull-down assays. Preparation of purified N-RNA template was done as described by Ogino and Banerjee (29). HA-tagged GST, PN40, and PC72 were expressed in HeLa cells as described above. Cells were treated with lysis buffer provided with the ProFound pull-down GST protein-protein interaction kit (Pierce) and incubated for 30 min in reverse transcriptase. After centrifugation at 13,000 rpm for 30 min, equal amounts of supernatants were mixed with 200 ng purified N-RNA template. GST pull-down assays were performed with the ProFound pull-down GST protein-protein interaction kit according to the manufacturer's protocol. Proteins in supernatants and eluted proteins from GST pull-down beads were detected by WB with anti-HA, anti-GST, or anti-N Abs, followed by gel electrophoresis.

In vitro transcription of the VSV genome RNA. The recombinant wild-type P and mutant P proteins (P1-10, P11-20, P21-30, and P31-40) were expressed as hexahistidine-tagged forms in Sf21 insect cells with the Bac-to-Bac baculovirus expression system (Invitrogen), purified with Ni-nitrilotriacetic acid agarose resin beads (QIAGEN), and used for in vitro transcription-reconstitution reactions with purified N-RNA (75 ng) and recombinant L (20 ng) and P (40 ng) as described by Ogino and Banerjee (29). ³²P-labeled transcripts were analyzed by electrophoresis in a 5% polyacrylamide gel containing 7 M urea, followed by autoradiography.

In vivo transcription of VSV minigenome RNA. BHK-21 cells in six-well plates were infected with vTF7-3 and transfected with plasmid pBS-N, pBS-L, or pVSV-CAT2 and a plasmid encoding HA-tagged P or mutant proteins. In the complementation assay, a plasmid encoding Myc-tagged P or P1-10 or a plasmid encoding HA-tagged P mutant protein was cotransfected with plasmid pBS-N, pBS-L, or pVSV-CAT2 (7). After 24 h, cell lysates were subjected to a chloramphenicol acetyltransferase (CAT) enzyme-linked immunosorbent assay (ELISA) for detection of the CAT expression level as described by Chen et al. (7). The expression levels of the wild-type and mutant P proteins were detected by WB with anti-HA and anti-Myc Abs.

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
The N-terminal 11 to 30 aa of the P protein are essential in keeping the N protein soluble. First, we constructed a series of mutant P cDNAs that, when expressed in HeLa cells, produced a battery of mutated P proteins (Fig. 1) that were used in all subsequent experiments. To directly map the region within the N-terminal domain of the P protein that interacts with the N protein to form the N⁰-P complex, we coexpressed the Myc-tagged N protein and the HA-tagged, N terminus truncated P protein (P1-40) in HeLa cells as described in Materials and Methods. The supernatants and pellets that were obtained by centrifugation at 13,000 rpm represented the soluble fraction and aggregated or RNA-bound N protein, respectively (12). As shown in Fig. 2A, when expressed alone, approximately 70% of the N protein was found in the pellet fraction (Fig. 2A, lane 2) as determined from the WB analyses, whereas about 70% of the N protein was found in the supernatant fraction along with P protein when it was coexpressed with the P protein (Fig. 2A, lane 3). In contrast, about 75% of the N protein remained in the pellet fraction when it was coexpressed with P1-40, suggesting that the N-terminal 40 aa are required for N⁰-P formation (Fig. 2A, lanes 2 and 3). Additional mutant P proteins were then coexpressed with the Myc-tagged N protein and compared with the observed effect of P1-40 on N protein solubility. As shown in Fig. 2B, HA-tagged P3A, PHinge, P161-210, and PII+III (Fig. 1) all were able to keep more than 53% of the N protein soluble (lanes 4 to 7), confirming that the N-terminal 40 aa are indeed required for its solubility and that phosphorylation of P protein is not required for N⁰-P complex formation. To further narrow down the region in P which can carry out this function, HA-tagged P1-20 and P1-10 (Fig. 1) were coexpressed with the N protein and the results show that P1-10 was also able to keep about 70% of the N protein soluble, which is similar to the wild-type P protein, but P1-20 was only able to keep 30% of the N protein soluble (Fig. 2C, lanes 4 and 5), indicating that residues 11 to 20 are essential for N protein solubility. To confirm this and test whether residues 21 to 40 are also required to keep the N protein soluble, we constructed three additional deletion mutant proteins, P11-20, P21-30, and P31-40, tagged with HA (Fig. 1) and coexpressed them with the N protein. As shown in Fig. 2D, P11-20 and P21-30 were able to keep only 36% and 40% of the N protein soluble, respectively, whereas P31-40 was similar to the wild-type P protein (lanes 4 to 6) and was able to keep 70% of the N protein soluble. If these results are taken together, it seems that N-terminal residues 11 to 30 of the P protein are essential for N⁰-P complex formation.

View larger version (21K): [in this window] [in a new window]

FIG. 1. Schematic representation of the wild-type P protein and the mutant P proteins used in this study. The wild-type P protein is divided into four regions designated I (aa 1 to 137), Hinge (aa 138 to 210), II (aa 211 to 244), and III (aa 245 to 265). The letters S at position 60, T at position 62, S at position 64, S at position 226, and S at position 227 represent the phosphorylation sites of the P protein. Wild-type P and the mutant P proteins were fused with an HA tag. The numbers represent amino acid positions.

View larger version (61K): [in this window] [in a new window]

FIG. 2. Amino acids 11 to 30 of the P protein are essential to keep N protein soluble. Myc-tagged N protein and HA-tagged P protein or mutant P proteins were coexpressed in HeLa cells as indicated. Supernatants (sup) from centrifugation at 13,000 rpm (A, B, C, and D) were detected by WB with anti-Myc, anti-HA, and anti-GAPDH monoclonal Abs. GAPDH was used as a loading control. (A) The pellet (ppt) was also detected by WB with an anti-Myc monoclonal Ab.

The N-terminal 40 aa of the P protein by themselves are capable of forming the N⁰-P complex. Next, we wanted to find out whether expressing only the N-terminal 40 aa will render the N protein soluble. Initially, we attempted to express the N-terminal 60 aa to avoid possible degradation of a smaller peptide of 40 aa in cells. However, we failed to detect any expression of the peptide in HeLa cells. We then constructed three chimeric proteins with two N-terminal peptides and one C-terminal peptide fused with GST and an HA tag as shown in Fig. 3A. HA-tagged GST, GST-1-40 (PN40), GST-1-60 (PN60), and GST-192-265 (PC72) were efficiently expressed when transfected into HeLa cells (data not shown). When these fusion peptides were coexpressed with the Myc-tagged N protein and the supernatants were analyzed for N protein by WB with anti-Myc Ab, both PN40 and PN60 were able to keep at least 70% of the N protein soluble whereas GST and PC72 were only able to keep 35% of the N soluble (Fig. 3B). From these results, it seems that both PN40 and PN60 could form N⁰-P complexes whereas PC72 possibly interacts with the N-RNA complex only as shown by Green et al. (15). To further confirm that PN40 binds to N⁰ and prevents the N protein to bind to cellular RNAs, increasing amounts of plasmids expressing PN40 or PC72 were coexpressed with the N protein and supernatant containing the soluble N protein after centrifugation at 13,000 rpm was further centrifuged at 35,000 rpm (see Materials and Methods) and analyzed for N protein by WB. When expressed alone, at least 80% of the N protein was pelleted during ultracentrifugation (U-ppt), which clearly indicated that it was bound to cellular RNAs (Fig. 3C, lane 1). We cannot, however, rule out the possibility that some small aggregated N complex also remained in the pellet. On the other hand, with increasing concentrations of PN40, nearly 90% of the N protein shifted to the U-sup fractions following ultracentrifugation, indicating that PN40 prevents N protein from binding to cellular RNAs (Fig. 3C, lanes 2 to 5). In contrast, almost 80% of the N protein remained in the pellet fraction with increasing concentrations of PC72 (Fig. 3C, lanes 6 to 9), indicating that PN40 fails to interact with the N-RNA complex whereas PC72 probably does (14). To directly demonstrate that PC72 indeed binds to the N-RNA complex, we used an N-RNA template purified from VSV virions (Fig. 4A and B) and studied its interaction with PN40 and PC72. Equal amounts of HeLa cell lysates expressing GST, PN40, and PC72 were mixed with 200 ng of purified N-RNA template for GST pull-down assays. As shown in Fig. 4C, PC72 was able to pull down the N-RNA template (lane 3, right side) but GST and PN40 were not (lanes 1 and 2, right side) although they had similar levels of expression (left side). Thus, it appears that PC72 bound to N protein prebound to RNA and PN40 interacted specifically with N⁰ when both were coexpressed with N in HeLa cells.

View larger version (32K): [in this window] [in a new window]

FIG. 3. The N-terminal 40 aa of the P protein are sufficient to keep N protein soluble. (A) Schematic representation of the N-terminal 40 and 60 aa and the C-terminal 72 aa fused with GST and an HA tag. Numbers represent amino acid positions. (B) Myc-tagged N and HA-tagged GST, GST-1-40 (PN40), GST-1-60 (PN60), or GST-192-265 (PC72) were coexpressed in HeLa cells as indicated. Supernatants (sup) from centrifugation at 13,000 rpm were detected by WB with anti-Myc, anti-HA, and anti-GAPDH monoclonal Abs. GAPDH was used as a loading control. (C) PN40 binds to N0, and PC72 binds to the N-RNA complex. Myc-tagged N protein was expressed alone or coexpressed with increasing concentrations of PN40 or PC72. Supernatants from centrifugation at 13,000 rpm were further centrifuged at 35,000 rpm (see Materials and Methods). N0 and the N-RNA complex were detected in U-sup or U-ppt by WB with anti-Myc Ab.

View larger version (26K): [in this window] [in a new window]

FIG. 4. The N-terminal 40-aa region is unable to bind to the N-RNA template in vitro. Purified RNP, N-RNA, and L-P complexes were analyzed by SDS-PAGE, followed by Coomassie brilliant blue (CBB) staining (A), and detected by WB with an anti-N polyclonal Ab (B). (C) PC72 interacted with the N-RNA template in vitro, but PN40 did not. Equal amounts of HeLa cell lysates expressing GST, PN40, or PC72 were detected by WB with anti-HA Ab (left side), and the same lysates were mixed with 200 ng of the purified N-RNA template in GST pull-down assays. Eluted GST beads were assayed by WB with anti-GST and anti-N Abs.

The N-terminal 40 aa of the P protein are not sufficient for VSV minigenome transcription in vivo, and the N-terminal 210 aa are required to make the minigenome competent for transcription. Next, we wanted to determine whether all of the N-terminal 40 aa are also required for VSV genome RNA transcription. First, we studied whether the mutant protein P1-10, P11-20, P21-30, or P31-40 would interact with the L protein to form an active RNA polymerase complex when coexpressed in HeLa cells. As shown in Fig. 5A, all of the mutant P proteins efficiently interacted with the L protein, since Ab-recognizing L protein brought down the wild-type P protein and all of the mutant P proteins (lanes 3 to 7). The expression levels of the P and L proteins were similar in the two cases (Fig. 5B), indicating that the RNA polymerase holoenzyme complex was formed in each case. We then tested all four mutant proteins for the ability to transcribe genome RNA in in vitro transcription-reconstitution assays. His-tagged mutant P proteins His-P1-10, His-P11-20, His-P21-30, and His-P31-40 were efficiently expressed in insect cells with recombinant baculovirus expressing the mutant proteins as described in Materials and Methods and were purified (Fig. 6A). The purified P proteins were then used in transcription-reconstitution reactions in vitro with purified L protein and the N-RNA template. As shown in Fig. 6B, with the N-RNA template alone or in the presence of L protein only, no transcription was observed (lanes 1 and 2) whereas the transcribed mRNAs (G, N, and P/M) were clearly detected in the presence of L and P, as well as with the four mutant proteins (lanes 3 to 7). The relative transcription activities of His-P1-10, His-P11-20, His-P21-30, and His-P31-40 were 103%, 88%, 72%, and 48% of that of wild-type P, which was taken as 100% (Fig. 6C). These results clearly demonstrated that all of the mutant proteins were active in supporting VSV genome transcription in vitro and that the N-terminal 30 aa are dispensable; His-P31-40, however, showed only 48% activity. To test whether these mutant proteins are active in transcription in vivo, we used the VSV minigenome transcription assay system previously used (7), as detailed in Materials and Methods. vTF7-3-infected BHK-21 cells were transfected with plasmids pVSV-CAT2, pBS-N, and pBS-L and a plasmid encoding HA-tagged P or the four mutant proteins, and cell lysates were subjected to a CAT ELISA for the detection of CAT expression levels. Surprisingly, all of the mutant proteins, including P1-10 and P31-40, which form the N⁰-P complex (Fig. 2), were unable to support VSV minigenome transcription in vivo (Fig. 6D, top). To detect transcription of the minigenome, it needs to be encapsidated by the N protein before transcription ensues; these results demonstrate that N⁰-P complex formation is necessary but not sufficient to encapsidate the genome RNA in vivo. All of the mutant proteins are transcriptionally active in vitro because they interact with the preformed N-RNA template and L protein. To address the possibility that regions beyond the N-terminal 40 aa are required to encapsidate VSV genome RNA, we used Myc-tagged P1-10, which is inactive in supporting minigenome transcription in vivo, and several mutant P proteins tagged with HA and performed a functional complementation assay. Myc-tagged wild-type P protein was used as a positive control. vTF7-3-infected BHK-21 cells were transfected with plasmids pVSV-CAT2, pBS-N, and pBS-L and a plasmid encoding Myc-P, Myc-P1-10, or HA-tagged mutant proteins PII+III, P161-210, and P76-137 (Fig. 1). As shown in Fig. 7A, all of the mutant proteins were unable to support minigenome transcription compared to the Myc-P protein (lanes 1 to 6) at similar levels of expression (Fig. 7B). However, when plasmids encoding Myc-P1-10 and HA-tagged PII+III or P161-210 were coexpressed in the presence of other supporting plasmids, the relative CAT expression levels were 82.3% and 40.7%, respectively (lanes 7 and 8), strongly suggesting that an additional region of the P protein is necessary for encapsidation of minigenome RNA. It is interesting that in the complementation with Myc-P1-10 and HA-P76-137, no CAT expression was detected (Fig. 7A, lane 9), which indicated that aa 76 to 137 are also required for the encapsidation of VSV genome RNA. We have previously demonstrated that P138-160 is active in supporting VSV minigenome transcription in vivo (7). Taken together, the findings indicate that the entire N-terminal 137 aa and the self-association domain (residues 161 to 210) are required for efficient encapsidation of VSV genome RNA.

View larger version (26K): [in this window] [in a new window]

FIG. 5. P1-10, P11-20, P21-30, and P31-40 all interact with L protein in vivo. HA-tagged wild-type P protein or mutant P proteins were coexpressed with Flag-tagged L protein (Flag-L) in HeLa cells as indicated. Protein expression was detected in lysates by WB with anti-HA and anti-Flag Abs (B). Immunoprecipitation (IP) was performed with anti-L polyclonal Ab, and immune complexes were detected by WB with anti-HA and anti-Flag Abs (A).

View larger version (41K): [in this window] [in a new window]

FIG. 6. The N-terminal 40-aa region is required for encapsidation of viral genome RNA. (A) Purified, recombinant, His-tagged wild-type P protein and mutant P proteins were analyzed by SDS-PAGE, followed by Coomassie brilliant blue (CBB) staining, and by WB with anti-His and anti-P Abs. (B) In vitro transcription reconstituted with an N-RNA template and recombinant P protein or mutant P proteins and L protein. The recombinant P protein or mutant P proteins and L protein were subjected to in vitro transcription with the N-RNA template. 32P-labeled transcripts were analyzed by urea-5% PAGE, followed by autoradiography. The positions of the viral mRNAs are indicated on the right. (C) The relative transcription activities (percent) of wild-type P protein and mutant P proteins in transcription reactions are indicated. The relative transcription activity of wild-type P protein was defined as 100%. (D) Roles of wild-type P protein and mutant P proteins in VSV minigenome transcription in vivo. BHK cells expressing T7 polymerase were transfected with pBS-N, pBS-L, pVSV-CAT2, and a plasmid encoding HA-tagged wild-type P protein or mutant P proteins as indicated. A CAT ELISA was performed with lysates of the transfected cells to measure relative CAT expression levels as viral minigenome transcription activities. The relative CAT expression level of the wild-type P protein was defined as 100%. The expression levels of the wild-type P protein and mutant P proteins were detected by WB with an anti-HA monoclonal Ab.

View larger version (29K): [in this window] [in a new window]

FIG. 7. Complementation assay of the ability of mutant proteins to support the transcription of the VSV minigenome in vivo. BHK cells expressing T7 polymerase were transfected with pBS-N, pBS-L, pVSV-CAT2, and a plasmid encoding the wild-type P protein or mutant P proteins as indicated. (A) A CAT ELISA was performed with lysates of transfected cells to measure relative CAT expression levels as viral minigenome transcription activities. The relative CAT expression level of the wild-type P protein was defined as 100%. (B) Expression levels of HA-tagged mutant proteins and the Myc-tagged P protein and P1-10 were detected by WB with anti-HA and anti-Myc monoclonal Abs, respectively.

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 
The N protein of VSV, as well as all viruses belonging to the NNS RNA virus family, plays a pivotal role in the life cycle of the virus. Not only does it encapsidate the genome RNA (and render it inaccessible to RNase action) which serves as the template for both transcription and replication to occur, but it also associates with the P protein to form a soluble N⁰-P complex, which is the required intermediate that provides the N protein to encapsidate newly replicated genome RNA. Additionally, the complex also interacts with the L protein to form the replicase complex that transcribes the genome or antigenome RNA end to end (32). It has been proposed that the putative replicase holoenzyme complex, i.e., L-N-P, initiates replication, which is followed by concomitant encapsidation of nascent RNA by N⁰-P (32). Thus, formation of the N⁰-P complex is crucial in the production of functional genomic and antigenomic templates. Several studies have been done previously to gain insight into the interaction of P protein with the N protein to form the N⁰-P complex, in particular, the roles of the N and C termini of the P protein in this process (36). However, whether formation of the N⁰-P complex is sufficient to encapsidate the genome RNA or there exists a separate region in P that is additionally required to regulate the N protein for encapsidation has not been investigated.

To address this possibility, we used a battery of mutant P proteins and studied their interactions with N protein expressed in HeLa cells. Our primary criteria were based on the fact the N protein, expressed alone, binds to cellular RNAs (5, 20, 21, 33, 27) and remains in the pellet fraction when the lysates are centrifuged (12). In contrast, when coexpressed with the wild-type P protein, a major of fraction, if not all, of N is rendered soluble and RNA free (8, 11, 24, 25, 31) and is found in the supernatant fraction. By these criteria, we have shown that the N-terminal 11 to 30 aa of the P protein, Indiana serotype, are required to keep N protein soluble (Fig. 2C and D). Importantly, the N-terminal peptide PN40 by itself is sufficient to form the N⁰-P complex (Fig. 3 and 4), whereas the C-terminal 72 aa of the P protein interact only with N protein that is already bound to RNA, i.e., the N-RNA complex (Fig. 4) (15). Since encapsidation of genome RNA by N protein is vital for subsequent transcription or replication of the N-RNA template, we used this criterion to further probe the presence of a putative encapsidation-facilitating domain within the P protein that would help the N⁰-P complex to carry out this function. With an in vivo minigenome transcription system, we demonstrated for the first time that N⁰-P complex formation is necessary but not sufficient to encapsidate the genome RNA. We showed that the mutant P protein P1-10, which is perfectly capable of forming the N⁰-P complex and highly active in an in vitro transcription assay, is incapable of carrying out minigenome transcription in vivo (Fig. 6B and D). In striking contrast, when a mutant protein lacking domains II and III (PII+III, which is transcription negative but N⁰-P positive) can efficiently complement the transcription function of P1-10 when added in trans (Fig. 7). These results strongly suggest that the encapsidation-facilitating domain resides beyond the N-terminal 40 aa, which is the minimum region required for N⁰-P complex formation. All of the N-terminal 210 aa (PII+III) containing the self-association domain (aa 161 to 210) seem to be essential for transcription complementation to occur since deletion of aa 161 to 210 reduced transcription complementation to 40%. Furthermore, deletion of aa 76 to 137 resulted in complete abrogation of transcription complementation, suggesting that the entire acidic domain is also critical to the encapsidation reaction. The precise boundaries of the N-terminal domain covering aa 1 to 137 required for encapsidation, however, need to be further investigated by additional deletion experiments. Taken together, it seems that the N-terminal 210 aa contain two overlapping domains, one N-terminal 40-aa domain for N⁰-P complex formation (NP40) and the other for encapsidation of genome RNA (NP1-210). It will be of interest to examine whether it is possible to rescue infectious virus by expressing P1-10 and P1-210 as two independent genes which would confirm our minigenome-based assay. It is noteworthy that NP1-210 contains the self-association domain of P (aa 161 to 210). Thus, it seems that within the N⁰-P complex, the self-association domain in P must be masked by the N protein to prevent it from oligomerizing and consequently binding to L to form a transcription complex (L-P₂). The L protein, on the other hand, may bind directly to the N⁰-P complex to form the putative replicase. The concerted action of the replicase complex and the N⁰-P complex may trigger the transcription-encapsidation reaction. The precise domain(s) within the N-terminal 1 to 210 aa of the P protein directly involved in the interaction with the L protein and the formation of an encapsidation-competent N⁰-P complex is currently under investigation.

A challenging question that remains is how the N⁰-P complex confers specificity to encapsidate only its cognate genome RNA. For example, the P protein of human parainfluenza virus type 3 (HPIV3) fails to form an N⁰-P complex with the N protein of VSV, and conversely, PN40 of VSV fails to form an N⁰-P complex with the N protein of HPIV3 (unpublished observations). These finding clearly demonstrate that the N and P proteins of NNS RNA viruses interact via a specific amino acid sequence or recognition fold, as well as recognize a specific cis-acting RNA sequence motif at the 5' end of the respective genome RNAs. Structural-biology studies would certainly provide insight into these interactive processes. Two recent publications of the crystal structure of N-RNA complexes of VSV (16) and rabies virus (1) purified from bacteria and insect cells, respectively, following expression of the N protein demonstrated that the RNA is tightly sequestered in a cavity at the interface between N protein lobes and shield it from the environment. The structure, however, does not reveal any sequence specificity in RNA recognition. It is noteworthy that a recent study of N⁰-P formation in Chandipura virus, which also belongs to the Rhabdoviridae family, demonstrated that the N protein in a monomeric form promotes specific viral genome RNA recognition. Once N oligomerizes or aggregates, it interacts with cellular RNA even in the presence of P (4). The P protein also interacts with leader RNA in its unphosphorylated form and initiates nucleocapsid assembly onto a nascent RNA chain (3). Thus, the interaction between P and leader RNA provides the specificity that successively recruits N protein onto RNA while itself being replaced. Whether VSV P also can keep N in a monomeric form and can recognize viral leader RNA in its unphosphorylated form needs to be studied.

N-P interaction studies with other NNS viruses show some similarity to our findings. It was shown for Sendai virus that the N-terminal half of the P protein is essential for N⁰-P complex formation but not for mRNA synthesis, and later studies demonstrated that this region could be further narrowed down to aa 33 to 41 (9, 10). The N⁰-P complex-forming domain in P of HPIV3 has been mapped within the N-terminal 40 aa (12). A similar study with rabies virus P protein by Mavrakis et al. (26) has recently shown that a peptide containing residues 4 to 40 remained associated with N within the N⁰-P complex following sequential protease digestion of the complex, confirming the requirement of this region for N⁰-P formation only. However, in each case, the precise region(s) of the P protein that is required for genome RNA encapsidation has not been determined.

In summary, we propose a model in which the P protein of VSV acts as a chaperone that keeps the N protein soluble and free from cellular RNA by interaction with a specific recognition motif in N⁰ through the N-terminal 40 aa (aa 11 to 30 are essential). For efficient encapsidation of the viral genome RNA, the N-terminal 137 aa and the self-association domain (aa 161 to 210) are required. However, the exact N-terminal boundary between aa 41 and 137 needed for encapsidation remains to be determined. Additional mutations are required to address this question. Once the viral genome RNA is transcribed for replication mediated by the replicase, the encapsidation of nascent RNA is initiated by the N protein from the N⁰-P complex via recognition of a specific cis-acting RNA motif present in the 5' end of the nascent RNA. The P protein probably dissociates from the N⁰-P complex during the encapsidation process or after it is complete. The P protein may subsequently bind to the L protein to form the RNA polymerase holoenzyme complex and bind to the newly formed N-RNA template via the C-terminal 72 aa or a shorter region of P to carry out its function as the RNA polymerase cofactor.

 
This work was supported by a grant from the National Institutes of Health (AI26585) to A.K.B.

 
* Corresponding author. Mailing address: Virology Section, Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195. Phone: (216) 444-0625. Fax: (216) 444-2998. E-mail: banerja{at}ccf.org

Published ahead of print on 3 October 2007.

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 

1
1. Albertini, A. A., A. K. Wernimont, T. Muziol, R. B. Ravelli, C. R. Clapier, G. Schoehn, W. Weissenhorn, and R. W. Ruigrok. 2006. Crystal structure of the rabies virus nucleoprotein-RNA complex. Science 313:360-363.[Abstract/Free Full Text]
2
2. Barik, S., and A. K. Banerjee. 1991. Cloning and expression of the vesicular stomatitis virus phosphoprotein gene in Escherichia coli: analysis of phosphorylation status versus transcriptional activity. J. Virol. 65:1719-1726.[Abstract/Free Full Text]
3
3. Basak, S., T. Raha, D. Chattopadhyay, A. Majumder, M. S. Shaila, and D. J. Chattopadhyay. 2003. Leader RNA binding ability of Chandipura virus P protein is regulated by its phosphorylation status: a possible role in genome transcription-replication switch. Virology 307:372-385.[CrossRef][Medline]
4
4. Bhattacharya, R., S. Basak, and D. J. Chattopadhyay. 2006. Initiation of encapsidation as evidenced by deoxycholate-treated nucleocapsid protein in the Chandipura virus life cycle. Virology 349:197-211.[CrossRef][Medline]
5
5. Bhella, D., A. Ralph, L. B. Murphy, and R. P. Yeo. 2002. Significant differences in nucleocapsid morphology within the Paramyxoviridae. J. Gen. Virol. 83:1831-1839.[Abstract/Free Full Text]
6
6. Chen, M., J. C. Cortay, I. R. Logan, V. Sapountzi, C. N. Robson, and D. Gerlier. 2005. Inhibition of ubiquitination and stabilization of human ubiquitin E3 ligase PIRH2 by measles virus phosphoprotein. J. Virol. 79:11824-11836.[Abstract/Free Full Text]
7
7. Chen, M., T. Ogino, and A. K. Banerjee. 2006. Mapping and functional role of the self-association domain of vesicular stomatitis virus phosphoprotein. J. Virol. 80:9511-9518.[Abstract/Free Full Text]
8
8. Curran, J., H. Homann, C. Buchholz, S. Rochat, W. Neubert, and D. Kolakofsky. 1993. The hypervariable C-terminal tail of the Sendai paramyxovirus nucleocapsid protein is required for template function but not for RNA encapsidation. J. Virol. 67:4358-4364.[Abstract/Free Full Text]
9
9. Curran, J., R. Boeck, N. Lin-Marq, A. Lupas, and D. Kolakofsky. 1995. Paramyxovirus phosphoproteins form homotrimers as determined by an epitope dilution assay, via predicted coiled coils. Virology 214:139-149.[CrossRef][Medline]
10
10. Curran, J., T. Pelet, and D. Kolakofsky. 1994. An acidic activation-like domain of the Sendai virus P protein is required for RNA synthesis and encapsidation. Virology 202:875-884.[CrossRef][Medline]
11
11. Davis, N. L., H. Arnheiter, and G. W. Wertz. 1986. Vesicular stomatitis virus N and NS proteins form multiple complexes. J. Virol. 59:751-754.[Abstract/Free Full Text]
12
12. De, B. P., M. A. Hoffman, S. Choudhary, C. C. Huntley, and A. K. Banerjee. 2000. Role of NH₂- and COOH-terminal domains of the P protein of human parainfluenza virus type 3 in transcription and replication. J. Virol. 74:5886-5895.[Abstract/Free Full Text]
13
13. Ding, H., T. J. Green, S. Lu, and M. Luo. 2006. Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus. J. Virol. 80:2808-2814.[Abstract/Free Full Text]
14
14. Errington, W., and P. T. Emmerson. 1997. Assembly of recombinant Newcastle disease virus nucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein. J. Gen. Virol. 78:2335-2339.[Abstract]
15
15. Green, T. J., S. Macpherson, S. Qiu, J. Lebowitz, G. W. Wertz, and M. Luo. 2000. Study of the assembly of vesicular stomatitis virus N protein: role of the P protein. J. Virol. 74:9515-9524.[Abstract/Free Full Text]
16
16. Green, T. J., X. Zhang, G. W. Wertz, and M. Luo. 2006 Structure of the vesicular stomatitis virus nucleoprotein-RNA complex. Science 5785:357-360.
17
17. Horikami, S. M., and S. A. Moyer. 1995. Alternative amino acids at a single site in the Sendai virus L protein produce multiple defects in RNA synthesis in vitro. Virology 211:577-582.[CrossRef][Medline]
18
18. Horikami, S. M., J. Curran, D. Kolakofsky, and S. A. Moyer. 1992. Complexes of Sendai virus NP-P and P-L proteins are required for defective interfering particle genome replication in vitro. J. Virol. 66:4901-4908.[Abstract/Free Full Text]
19
19. Howard, M., and G. Wertz. 1989. Vesicular stomatitis virus RNA replication: a role for the NS protein. J. Gen. Virol. 70:2683-2694.[Abstract/Free Full Text]
20
20. Iseni, F., A. Barge, F. Baudin, D. Blondel, and R. W. Ruigrok. 1998. Characterization of rabies virus nucleocapsids and recombinant nucleocapsid-like structures. J. Gen. Virol. 79:2909-2919.[Abstract]
21
21. Karlin, D., S. Longhi, and B. Canard. 2002. Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association. Virology 302:420-432.[CrossRef][Medline]
22
22. La Ferla, F. M., and R. W. Peluso. 1989. The 1:1 N-NS protein complex of vesicular stomatitis virus is essential for efficient genome replication. J. Virol. 63:3852-3857.[Abstract/Free Full Text]
23
23. Majumder, A., S. Basak, T. Raha, S. P. Chowdhury, D. Chattopadhyay, and S. Roy. 2001. Effect of osmolytes and chaperone-like action of P-protein on folding of nucleocapsid protein of Chandipura virus. J. Biol. Chem. 276:30948-30955.[Abstract/Free Full Text]
24
24. Masters, P. S., and A. K. Banerjee. 1988. Resolution of multiple complexes of phosphoprotein NS with nucleocapsid protein N of vesicular stomatitis virus. J. Virol. 62:2651-2657.[Abstract/Free Full Text]
25
25. Masters, P. S., and A. K. Banerjee. 1988. Complex formation with vesicular stomatitis virus phosphoprotein NS prevents binding of nucleocapsid protein N to nonspecific RNA. J. Virol. 62:2658-2664.[Abstract/Free Full Text]
26
26. Mavrakis, M., S. Mehouas, E. Real, F. Iseni, D. Blondel, N. Tordo, and R. W. Ruigrok. 2006. Rabies virus chaperone: identification of the phosphoprotein peptide that keeps nucleoprotein soluble and free from non-specific RNA. Virology 349:422-429.[CrossRef][Medline]
27
27. Mavrakis, M., L. Kolesnikova, G. Schoehn, S. Becker, and R. W. Ruigrok. 2002. Morphology of Marburg virus NP-RNA. Virology 296:300-307.[CrossRef][Medline]
28
28. Mellon, M. G., and S. U. Emerson. 1978. Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus. J. Virol. 27:560-567.[Abstract/Free Full Text]
29
29. Ogino, T., and A. K. Banerjee. 2007. Unconventional mechanism of mRNA capping by the RNA-dependent RNA polymerase of vesicular stomatitis virus. Mol. Cell 25:85-97.[CrossRef][Medline]
30
30. Pattnaik, A. K., L. Hwang, T. Li, N. Englund, M. Mathur, T. Das, and A. K. Banerjee. 1997. Phosphorylation within the amino-terminal acidic domain I of the phosphoprotein of vesicular stomatitis virus is required for transcription but not for replication. J. Virol. 71:8167-8175.[Abstract]
31
31. Peluso, R. W. 1988. Kinetic, quantitative, and functional analysis of multiple forms of the vesicular stomatitis virus nucleocapsid protein in infected cells. J. Virol. 62:2799-2807.[Abstract/Free Full Text]
32
32. Qanungo, K. R., D. Shaji, M. Mathur, and A. K. Banerjee. 2004. Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA. Proc. Natl. Acad. Sci. USA 101:5952-5957.[Abstract/Free Full Text]
33
33. Schoehn, G., F. Iseni, M. Mavrakis, D. Blondel, and R. W. Ruigrok. 2001. Structure of recombinant rabies virus nucleoprotein-RNA complex and identification of the phosphoprotein binding site. J. Virol. 75:490-498.[Abstract/Free Full Text]
34
34. Smallwood, S., K. W. Ryan, and S. A. Moyer. 1994. Deletion analysis defines a carboxyl-proximal region of Sendai virus P protein that binds to the polymerase L protein. Virology 202:154-163.[CrossRef][Medline]
35
35. Spehner, D., R. Drillien, and P. M. Howley. 1997. The assembly of the measles virus nucleoprotein into nucleocapsid-like particles is modulated by the phosphoprotein. Virology 232:260-268.[CrossRef][Medline]
36
36. Takacs, A. M., T. Das, and A. K. Banerjee. 1993. Mapping of interacting domains between the nucleocapsid protein and the phosphoprotein of vesicular stomatitis virus by using a two-hybrid system. Proc. Natl. Acad. Sci. USA 90:10375-10379.[Abstract/Free Full Text]

---

Journal of Virology, December 2007, p. 13478-13485, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01244-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Li, M., Schmitt, P. T., Li, Z., McCrory, T. S., He, B., Schmitt, A. P. (2009). Mumps Virus Matrix, Fusion, and Nucleocapsid Proteins Cooperate for Efficient Production of Virus-Like Particles. J. Virol. 83: 7261-7272 [Abstract] [Full Text]  

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, M. Articles by Banerjee, A. K. Search for Related Content PubMed PubMed Citation Articles by Chen, M. Articles by Banerjee, A. K.