Files in this Data Supplement:

• Supplemental file 1 - Alignment Fig. S1 (Alignment of Virp1 ortholog nucleotide sequences.)
  Alignment Fig. S2 (Alignment of Virp1 protein nucleotide sequences.)
  Zipped PDF document, 68K.
• Supplemental file 2 - Fig. S1 (Analysis of Virp1 expression in various Solanaceae species.)
  Fig. S2 (GFP-virp1 fusion protein was not found capable of local spread.)
  Fig. S3 (Northern analysis of transgenic llines transformed with a hairpin construct against Virp1.) Fig. S4 (sRNAs produced by transgenic N. benthiamiana and lines carrying the Virp1-hairpin construct.)
  Fig. S5 (Infection analysis of N. tabacum protoplasts inoculated with PSTVd.)
  Fig. S6 (Western detection of Virp1 from transgenic lines.)
  Zipped PDF document, 573K.
• Supplemental file 3 - Legends to alignment and supplemental figures Table S1 (Nucleotide sequence identity of Virp1 homologs in different Solanaceae species.) Table S2 (Amino acid sequence identity of Virp1 homologs in different Solanaceae species.)
  MS Word document, 58K.