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Journal of Virology, July 2007, p. 7327, Vol. 81, No. 13
0022-538X/07/$08.00+0 doi:10.1128/JVI.00871-07
| AUTHOR'S CORRECTION |
Department for Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany
Volume 81, no. 9, p. 4591-4603, 2007. Page 4593, Materials and Methods, Plasmid construction, lines 4 to 16: We have discovered that there is an error in the description of the plasmid construction of fluorochrome gene-tagged reporter plasmids. We performed two separate PCRs, using antisense primer Alinker_Xba_Pme_NS5A_aa383 (5'-TTAAACCCAGGTCTAGAACCGCTCGAGGGGGGCTGGCCAAAG-3') and sense primer S6660 (5'-CAGGACTGACCACTGACAATCTG-3') or sense primer Slinker_Xba_Pme_NS5A_aa383 (5'-TCTAGACCTGGGTTTAAACGTGATGCAGGCTCGTCCACGGG-3') and antisense primer A7759 (5'-CAGACTCCAGGTCCGGATCTCCAGGC-3') for insertion of the linker at NS5A amino acid position 383 and antisense primer Alinker_Xba_Pme_NS5A_aa378 (5'-TTAAACCCAGGTCTAGACTGGCCAAAGGTCTTGATGGCCAG-3') and sense primer S6660 or sense primer Slinker_Xba_Pme_NS5A_aa378 (5'-TCTAGACCTGGGTTTAAACGTCCCCCCTCGAGCGGTGATGCAG-3') and antisense primer A7759 for insertion at NS5A amino acid position 378, respectively. Amplicons were combined by a second PCR resulting in fragments that comprise the complete NS5A coding region plus linker, which were SanDI/RsrII digested and transferred into pFKi389LucNS3-3'_dg_JFH.
We apologize for any inconvenience this may have caused to other investigators.
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