Skip to main page content

• HOME
• Current Issue
• Archive
• Alerts
• About ASM
• Contact us
• Tech Support
• Journals.ASM.org

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carter, J. R. Articles by Dutch, R. E. Search for Related Content PubMed PubMed Citation Articles by Carter, J. R. Articles by Dutch, R. E.

 Previous Article  |  Next Article 

Journal of Virology, June 2005, p. 7922-7925, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7922-7925.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Role of N-Linked Glycosylation of the Hendra Virus Fusion Protein

James Richard Carter, Cara Theresia Pager, Stephen Derrick Fowler, and Rebecca Ellis Dutch^*

Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky 40536-0298

Received 2 December 2004/ Accepted 8 February 2005

Top Abstract Text References

 
The Hendra virus fusion (F) protein contains five potential sites for N-linked glycosylation in the ectodomain. Examination of F protein mutants with single asparagine-to-alanine mutations indicated that two sites in the F₂ subunit (N67 and N99) and two sites in the F₁ subunit (N414 and N464) normally undergo N-linked glycosylation. While N-linked modification at N414 is critical for protein folding and transport, F proteins lacking carbohydrates at N67, N99, or N464 remained fusogenically active. As N464 lies within heptad repeat B, these results contrast with those seen for several paramyxovirus F proteins.

Top Abstract Text References

 
Hendra virus, a newly emerged paramyxovirus in the Henipavirus genus, has two glycoproteins: the fusion (F) protein, which promotes membrane fusion (3), and the attachment protein, G. The Hendra virus F protein shows characteristics of type I viral fusion proteins (Fig. 1), including heptad repeats (HR) critical for promotion of membrane fusion (reviewed in reference 5) and posttranslational cleavage of the precursor form (F₀) to the fusogenic heterodimer, F₁+F₂ (13). The Hendra virus F ectodomain contains five N-X-S/T motifs for the potential addition of N-linked carbohydrates, two in F₁ and three in F₂. However, lectin binding analysis found no evidence that the F₁ subunit contained N-linked carbohydrates (9). This is surprising for several reasons. First, the frequency of N-linked site usage is approximately 90% (7). Second, N-linked glycosylation in the transmembrane-proximal heptad repeat (HRB) is important for the folding or membrane fusion activity of several paramyxovirus F proteins (8, 18), and one potential N-linked site (N464) in the Hendra virus F₁ subunit is within HRB. Finally, the closely related Nipah virus F protein was recently reported to contain N-linked carbohydrates in both F₁ and F₂ (10). We therefore examined N-linked carbohydrate additions to the Hendra virus F protein.

View larger version (6K): [in this window] [in a new window]

FIG. 1. Schematic of the Hendra virus fusion protein. FP, fusion peptide; HR, heptad repeat; TM, transmembrane domain; S-S, disulfide bond. The positions of the potential N-linked glycosylation sites and mutations produced are shown.

Six asparagine-to-alanine mutants in potential glycosylation sites were created: three in F₂ (N64A, N67A, and N99A) and three in F₁ (N414A, N464A, and N485A) (Fig. 1). While N485 was previously identified as a potential site for N-linked glycosylation (9), a proline at position 486 should preclude N-linked glycosylation. To analyze expression and mobility, the wild-type (wt) or mutant Hendra virus F genes in the pCAGGS expression plasmid (12) were transfected into Vero cells and metabolic labeling and immunoprecipitation were performed as previously described (13). Samples were separated on a 10% polyacrylamide gel. The wt Hendra virus F protein (Fig. 2A, lane 1) is processed from the precursor, F₀, to the mature form, F₁+F₂. No proteolytic cleavage was seen for mutants N64A and N414A (Fig. 2A, lanes 2 and 5), although a mobility shift was observed for N414A, suggesting that these mutations disrupt folding and processing. Compared to the wt protein, mobility shifts in F₀ and F₂ are seen for N67A and N99A (Fig. 2A, lanes 3 and 4), indicating that these sites in F₂ are normally N-link glycosylated. The mobility difference in the F₂ subunits between N67A and N99A is likely due to variation in branching of the glycan. Both F₀ and F₁ in HRB mutant N464A show faster mobility than in the wt protein (Fig. 2A, lane 6), suggesting that N464 is also N-link glycosylated. Finally, no change in mobility is seen for N485A (Fig. 2A, lane 7), confirming that this site is not glycosylated. N-glycosidase F treatment (Calbiochem), performed as described previously (14), followed by analysis on a 15% polyacrylamide gel showed shifts in all three forms of the wt Hendra virus F protein, confirming that N-linked carbohydrates are present on the F₀, F₁, and F₂ subunits (Fig. 2B). The subunit mobilities after N-glycosidase F treatment are similar in wt and mutant proteins, consistent with mobility changes resulting from differences in N-linked carbohydrates.

View larger version (71K): [in this window] [in a new window]

FIG. 2. Examination the wt Hendra virus F protein and the six mutated forms for expression, mobility, and digestion by N-glycosidase F. (A) Hendra virus wt and mutant F proteins were expressed in Vero cells, starved, labeled with Tran-[35S] for 30 min, and chased for 3 h. Samples were prepared as described previously (13), separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and analyzed using the STORM imaging system. (B) Effect of N-glycosidase F treatment. Hendra virus wt and mutant F proteins were expressed in Vero cells as described previously. Following immunoprecipitation, samples were digested overnight with N-glycosidase F (0.2 U) (14), separated by 15% SDS-PAGE, and analyzed via the STORM PhosphorImager system.

To further explore the cleavage-deficient phenotypes, two additional mutations were created. The Hendra virus F mutant S66A shows mobility and proteolytic cleavage similar to that of the wt protein (Fig. 2A, lanes 8 and 9), suggesting that asparagine 64 is not N-link modified but is required for proper folding and processing. Hendra virus F mutant T416A displays mobility and lack of proteolytic cleavage similar to those of Hendra virus F mutant N414A (Fig. 2A, lanes 5 and 10), indicating that glycosylation at N414 is required for proper processing.

As the removal of N-linked glycans from viral fusion proteins can alter fusogenic activity (8, 18), syncytium formation in BHK-21F cells was examined (Fig. 3A) (6). While no syncytia were observed when the wt or mutant Hendra virus F proteins were expressed alone (data not shown and reference 3), coexpression of wt Hendra virus F protein or the mutants S66A, N67A, N99A, or N464A with the Hendra virus G protein produced syncytium formation (Fig. 3A). The N64A, N414A, and T416A mutants did not form syncytia when coexpressed with the Hendra virus G protein (Fig. 3A), consistent with the cleavage deficiencies observed (Fig. 2).

View larger version (90K): [in this window] [in a new window]

FIG. 3. Membrane fusion promoted by the Hendra virus F wt and mutant proteins. (A) Syncytium formation. Hendra virus wt and mutant F proteins were coexpressed in BHK-21F cells with the Hendra virus G attachment protein. Approximately 18 h posttransfection, photographs were taken at a magnification of x10 using the Nikon Eclipse TS100 microscope outfitted with a Nikon Coolpix 995 digital camera. (B) Luciferase fusion assay of Hendra virus wt and mutant F proteins. pCAGGS Hendra virus F wt or the indicated mutants, along with pCAGGS Hendra virus G and a plasmid containing the luciferase gene under the control of the T7 promoter, were transfected into Vero cells. BSR cells, which stably express the T7 polymerase (4), were overlaid onto the F- and G-expressing cells, and the mixed cell populations were incubated at 37°C for 3 h. Cells were lysed and analyzed for luciferase activity on a luminometer (Molecular Devices). Samples used were averages of triplicates and are representative of five separate experiments. G, Hendra virus G protein alone; F, Hendra virus F protein alone; V, the pCAGGS vector alone.

To more accurately quantitate fusion, a luciferase reporter gene assay was performed (Fig. 3B) (15). No fusion was detected when the Hendra virus F or G proteins were expressed alone or when the folding-defective mutants N64A, N414A, or T416A were coexpressed with Hendra virus G. In the presence of Hendra virus G, S66A resulted in no statistically significant change in fusion compared to the wt protein. Removal of carbohydrates in F₂ (N67A and N99A) resulted in F proteins that were fusogenic, though the extent of fusion showed a statistically significant decrease. Surprisingly, removal of the carbohydrate in HRB (N464A) resulted in increased fusion (150% of that of the wt on average).

As lowered surface expression can decrease fusion activity (6), a biotinylation assay with Vero cells transiently expressing wt or mutant Hendra virus F proteins was performed according to the manufacturer's instructions (Pierce), with the same number of cells being used for each biotinylation. The mature F₁ subunit and some precursor F₀ are present on the cell surface for wt Hendra virus F and the mutants N67A, N99A, and N464A (Fig. 4A, lanes 1, 3, 4 and 6), consistent with reports of uncleaved F protein in Hendra virus virions (9). Only uncleaved N64A and N414A are present on the cell surface (Fig. 4A, lanes 2 and 5), consistent with the cleavage defects previously demonstrated. Quantitation of F₁ from four separate experiments using ImageQuant (Amersham) showed 40% (N67A), 70% (N99A), and 50% (N464A) reductions in surface expression. The decrease in fusion for N67A or N99A correlates with a decrease in surface expression. In contrast, higher levels of fusion are seen with N464A (Fig. 3B), even with lowered surface density, confirming the hyperfusogenic nature of this mutant.

View larger version (28K): [in this window] [in a new window]

FIG. 4. Cell surface expression and examination of possible additional modifications to the Hendra virus F protein. (A) Biotinylation assay of wt and mutant Hendra virus proteins for cell surface expression. Vero cells were transfected and metabolically labeled, and biotinylation on an identical numbers of cells was performed as described in the text. The biotinylated proteins were separated by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analyzed via the STORM PhosphorImager system. (B) Comparison of mobilities of Hendra virus F and SV5 F proteins transiently expressed in Vero cells after tunicamycin treatment (T) or N-glycosidase F digestion (P). For tunicamycin samples, cells were preincubated in 1 µg/ml tunicamycin, labeled for 30 min, and chased for 3 h. N-glycosidase F digestions were performed as described previously (14). Samples were separated on a 10% acrylamide gel and analyzed using the STORM PhosphorImager system.

Other viral glycoproteins undergo additional posttranslational modifications, such as O-linked glycosylation (2), acylation (17), or phosphorylation (11). To determine whether Hendra virus F has additional posttranslational modifications, protein mobility after synthesis in the presence of tunicamycin (1 µg/ml; Calbiochem), which inhibits N-linked glycosylation in the endoplasmic reticulum (16), was compared to mobility after N-glycosidase F treatment. Tunicamycin treatment gave a single band (Fig. 4B; lane 2), consistent with the requirement for N-linked glycosylation at N414 for protein processing. N-glycosidase F-treated Hendra virus F₀ runs slower than F₀ from the tunicamycin-treated cells (Fig. 4B, lanes 2 and 4), suggesting that additional modifications occur during transport through the secretory pathway. The SV5 F protein, which requires N-linked glycosylation for folding (1) but has no other identified modifications, had mobilities similar to those of the F₀ precursor either synthesized with tunicamycin present or after treatment with N-glycosidase F (Fig. 4B, lanes 6 and 8).

Our studies of the Hendra virus F protein clearly demonstrate that both the F₂ (at N67 and N99) and F₁ (at N414 and N464) subunits contain N-linked carbohydrates, in contrast to lectin binding studies which did not detect N-linked modifications in the F₁ subunit (9). Our findings indicate that the Hendra virus and Nipah virus F proteins utilize N-linked carbohydrates in a similar manner: the sites of addition match (10), glycosylation at residue 414 is critical for processing (Fig. 2A) (10), and removal of the N-linked carbohydrate in HRB (N464A) gives a significant decrease of cell surface expression (10). Many paramyxovirus F proteins contain a carbohydrate within HRB. This modification is required for SV5 F protein cleavage and cell surface expression (1) and is important for fusion promotion of the Newcastle disease virus and respiratory syncytial virus F proteins (8, 18). In contrast, the Nipah virus mutant lacking HRB glycosylation promoted efficient syncytium formation even with an 80% decrease in surface expression (10). Removal of the HRB carbohydrate from the Hendra virus F protein results in both decreased surface expression and increased fusion activity, clearly shown in the quantitative fusion assay (Fig. 3B). These results suggest mechanistic differences in fusion promotion between the Hendra virus and Nipah virus F proteins and F proteins from other paramyxoviruses.

 
We thank Lin-Fa Wang of the Australian Animal Health Laboratory for the Hendra virus F and G wt plasmids and Robert A. Lamb (HHMI, Northwestern University) for the SV5 F plasmid and antibodies. The BSR cells were kindly provided by Karl-Klaus Conzelmann, Max-von Pettenkofer-Institut. We thank Dava S. West for excellent technical assistance and Richard O. McCann, Robert Geraghty, Daniel Noonan, as well as the members of the Dutch laboratory, for critical review of the manuscript.

This study was supported by NIAID grant A151517 to R.E.D.

 
* Corresponding Author: Department of Molecular and Cellular Biochemistry University of Kentucky, 800 Rose Street, UKMC MN606 Lexington, KY 40536-0298. Phone: (859) 323-1795. Fax: (859) 323-1037. E-mail:rdutc2{at}uky.edu.

Top Abstract Text References

 

1
1. Bagai, S., and R. A. Lamb. 1995. Individual roles of N-linked oligosaccharide chains in intracellular transport of the paramyxovirus SV5 fusion protein. Virology 209:250-256.[CrossRef][Medline]
2
2. Bernstein, H. B., S. P. Tucker, E. Hunter, J. S. Schutzbach, and R. W. Compans. 1994. Human immunodeficiency virus type 1 envelope glycoprotein is modified by O-linked oligosaccharides. J. Virol. 68:463-468.[Abstract/Free Full Text]
3
3. Bossart, K. N., L. F. Wang, B. T. Eaton, and C. C. Broder. 2001. Functional expression and membrane fusion tropism of the envelope glycoproteins of Hendra virus. Virology 290:121-135.[CrossRef][Medline]
4
4. Buchholz, U., S. Finke, and K. Conzelmann. 1999. Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J. Virol. 73:251-259.[Abstract/Free Full Text]
5
5. Dutch, R. E., T. S. Jardetzky, and R. A. Lamb. 2000. Virus membrane fusion proteins: biological machines that undergo a metamorphosis. Biosci. Rep. 20:597-612.[CrossRef][Medline]
6
6. Dutch, R. E., S. B. Joshi, and R. A. Lamb. 1998. Membrane fusion promoted by increasing surface densities of the paramyxovirus F and HN proteins: comparison of fusion reactions mediated by simian virus 5 F, human parainfluenza virus type 3 F, and influenza virus HA. J. Virol. 72:7745-7753.[Abstract/Free Full Text]
7
7. Gavel, Y., and G. von Heijne. 1990. Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein Eng. 3:433-442.[Abstract/Free Full Text]
8
8. McGinnes, L., T. Sergel, J. Reitter, and T. Morrison. 2001. Carbohydrate modification of the NDV fusion protein heptad repeat domains influence maturation and fusion activity. Virology 283:332-342.[CrossRef][Medline]
9
9. Michalski, W. P., G. Crameri, L. Wang, B. J. Shiell, and B. Eaton. 2000. The cleavage activation and sites of glycosylation in the fusion protein of Hendra virus. Virus Res. 69:83-93.[CrossRef][Medline]
10
10. Moll, M., A. Kaufmann, and A. Maisner. 2004. Influence of N-glycans on processing and biological activity of the Nipah virus fusion protein. J. Virol. 78:7274-7278.[Abstract/Free Full Text]
11
11. Muhlberger, E., M. Weik, V. E. Volchkov, H. D. Klenk, and S. Becker. 1999. Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems. J. Virol. 73:2333-2342.[Abstract/Free Full Text]
12
12. Niwa, H., K. Yamamura, and J. Miyazaki. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 8:193-199.
13
13. Pager, C. T., M. A. Wurth, and R. E. Dutch. 2004. Subcellular localization and calcium and pH requirements for proteolytic processing of the Hendra virus fusion protein. J. Virol. 78:9154-9163.[Abstract/Free Full Text]
14
14. Paterson, R. G., and R. A. Lamb. 1993. The molecular biology of influenza viruses and paramyxoviruses, p. 35-73. In A. Davidson and R. M. Elliott (ed.), Molecular virology: a practical approach. IRL Oxford University Press, Oxford, England.
15
15. Russell, C., K. L. Kantor, T. S. Jardetzky, and R. A. Lamb. 2003. A dual-functional paramyxovirus F protein regulatory switch segment: activation and membrane fusion. J. Cell Biol. 163:363-374.[Abstract/Free Full Text]
16
16. Tordai, A., L. F. Brass, and E. W. Gelfand. 1995. Tunicamycin inhibits the expression of functional thrombin receptors on human T-lymphoblastoid cells. Biochem. Biophys. Res. Commun. 206:857-862.[CrossRef][Medline]
17
17. Ujike, M., K. Nakajima, and E. Nobusawa. 2004. Influence of acylation sites of influenza B virus hemagglutinin on fusion pore formation and dilation. J. Virol. 78:11536-11543.[Abstract/Free Full Text]
18
18. Zimmer, G., I. Trotz, and G. Herrler. 2001. 2001. N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus. J. Virol. 75:4744-4751.[Abstract/Free Full Text]

---

Journal of Virology, June 2005, p. 7922-7925, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7922-7925.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Vigerust, D. J., Ulett, K. B., Boyd, K. L., Madsen, J., Hawgood, S., McCullers, J. A. (2007). N-Linked Glycosylation Attenuates H3N2 Influenza Viruses. J. Virol. 81: 8593-8600 [Abstract] [Full Text]  
• Gardner, A. E., Dutch, R. E. (2007). A Conserved Region in the F2 Subunit of Paramyxovirus Fusion Proteins Is Involved In Fusion Regulation. J. Virol. 81: 8303-8314 [Abstract] [Full Text]  
• Aguilar, H. C., Matreyek, K. A., Choi, D. Y., Filone, C. M., Young, S., Lee, B. (2007). Polybasic KKR Motif in the Cytoplasmic Tail of Nipah Virus Fusion Protein Modulates Membrane Fusion by Inside-Out Signaling. J. Virol. 81: 4520-4532 [Abstract] [Full Text]  
• Schowalter, R. M., Smith, S. E., Dutch, R. E. (2006). Characterization of Human Metapneumovirus F Protein-Promoted Membrane Fusion: Critical Roles for Proteolytic Processing and Low pH. J. Virol. 80: 10931-10941 [Abstract] [Full Text]  
• Aguilar, H. C., Matreyek, K. A., Filone, C. M., Hashimi, S. T., Levroney, E. L., Negrete, O. A., Bertolotti-Ciarlet, A., Choi, D. Y., McHardy, I., Fulcher, J. A., Su, S. V., Wolf, M. C., Kohatsu, L., Baum, L. G., Lee, B. (2006). N-Glycans on Nipah Virus Fusion Protein Protect against Neutralization but Reduce Membrane Fusion and Viral Entry.. J. Virol. 80: 4878-4889 [Abstract] [Full Text]  
• Meulendyke, K. A., Wurth, M. A., McCann, R. O., Dutch, R. E. (2005). Endocytosis Plays a Critical Role in Proteolytic Processing of the Hendra Virus Fusion Protein. J. Virol. 79: 12643-12649 [Abstract] [Full Text]  
• Pager, C. T., Dutch, R. E. (2005). Cathepsin L Is Involved in Proteolytic Processing of the Hendra Virus Fusion Protein. J. Virol. 79: 12714-12720 [Abstract] [Full Text]  

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carter, J. R. Articles by Dutch, R. E. Search for Related Content PubMed PubMed Citation Articles by Carter, J. R. Articles by Dutch, R. E.