Files in this Data Supplement:

• Supplemental file 1 - Fig. S1. Expression levels of endogenous Notch1 and Notch2 in HaCaT cells and stable expression levels of Jagged1 and HA-tagged Delta1 in HaCaT-Jagged1 and HaCaT Delta cell lines, respectively. Also shown are levels of phospho-PKB/AKT (ser-473) in subcutaneous tumors generated by HaCaT cells expressing a combination of genes.
  Fig. S2. Jagged1 expression leads to induction PI3K-mediated generation of anoikis resistance.
  Fig. S3. Expression level of plakoglobin in HaCaT cells stably expressing mock vector (HaCaT-Neo) or Jagged1 (HaCaT-Jag1) at 20 h postwounding. The changes in expression of EMT markers in SiHa cells at 40 h in an in vitro wound healing assay are shown.
  Fig. S4. Jagged1-induced PI3K activation and anoikis resistance is independent of CSL-mediated Notch signaling.
  Fig. S5. Tumor volume in nude mice after 3 weeks of subcutaneous infection of HaCaT cells stably expressing mock vector (Neo) or C-terminal PDZ-binding motif mutant Jagged1. PDF document, 200K.
• Supplemental file 2 - : Table. S1. C-terminal PDZ-ligand motif mutant Jagged1 cooperates with HPV-16 E6 and E7 in transformation of HaCaT cells in in vitro soft agar colony formation assays.
  Table S2. List of genes either up- or downregulated in the microarray analysis of HaCaT cells stably expressing either Jagged1 or Delta1.

  PDF document, 325K.