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Aureusvirus P14 Is an Efficient RNA Silencing Suppressor That Binds Double-Stranded RNAs without Size Specificity
J. Virol. Mérai et al. 79: 7217

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Files in this Data Supplement:

  • Supplemental file 1 - Materials S1. P14 is not expressed in PoLV[Greek capital letter Delta], 14-inoculated tissues, while it is expressed to high levels in P14-agroinfiltrated leaves.
    Materials S2. Description of experiments that were carried out to prove that the long dsRNA binding activity of P14 extract was specific.
    Materials S3. Description of experiments that were carried out to study the effect of P14 on virus-induced cell-autonomous silencing.
    Materials S4. Description of how we aligned the ORF4- and ORF5-encoded RNAs and the deduced proteins of tombusviruses and aureusviruses.
    Table S1.Tombusvirus and aureusvirus strains that were used for RNA and protein alignments.
    MS Word document, 32K.
  • Supplemental file 2 - Fig. S1. P14 is expressed to high levels in PoLV-infected and in P14-infiltrated leaves.
    PowerPoint presentation, 549K.
  • Supplemental file 2 - Fig. S2. Multiple sequence alignments of RNA sequences of available aureusvirus and tombusvirus ORF4 regions.
    Fig. S3. Multiple sequence alignments of amino acid sequences deduced from available aureusvirus and tombusvirus ORF4s.
    Fig. S4. Consensus trees of MP and suppressor proteins of aureusvirus and tombusvirus.
    MS Word document 2.5MB.




This Article
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