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Journal of Virology, January 2004, p. 1050-1054, Vol. 78, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.2.1050-1054.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of an Envelope Protein from the FRD Family of Human Endogenous Retroviruses (HERV-FRD) Conferring Infectivity and Functional Conservation among Simians

Sandra Blaise,¹ Alessia Ruggieri,² Marie Dewannieux,¹ François-Loic Cosset,² and Thierry Heidmann^1*

Unité des Rétrovirus Endogènes et Eléments Rétroïdes des Eucaryotes Supérieurs, UMR 8122 CNRS, Institut Gustave Roussy, 94805 Villejuif,¹ Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Ecole Normale Supérieure de Lyon, 69364 Lyon, France²

Received 15 July 2003/ Accepted 30 September 2003

Top Abstract Introduction References

 
A member of the HERV-W family of human endogenous retroviruses (HERV) had previously been demonstrated to encode a functional envelope which can form pseudotypes with human immunodeficiency virus type 1 virions and confer infectivity on the resulting retrovirus particles. Here we show that a second envelope protein sorted out by a systematic search for fusogenic proteins that we made among all the HERV coding envelope genes and belonging to the HERV-FRD family can also make pseudotypes and confer infectivity. We further show that the orthologous envelope genes that were isolated from simians—from New World monkeys to humans—are also functional in the infectivity assay, with one singular exception for the gibbon HERV-FRD gene, which is found to be fusogenic in a cell-cell fusion assay, as observed for the other simian envelopes, but which is not infectious. Sequence comparison of the FRD envelopes revealed a limited number of mutations among simians, and one point mutation—located in the TM subunit—was shown to be responsible for the loss of infectivity of the gibbon envelope. The functional characterization of the identified envelopes is strongly indicative of an ancestral retrovirus infection and endogenization, with some of the envelope functions subsequently retained in evolution.

Top Abstract Introduction References

 
Human endogenous retroviruses (HERV) comprise approximately 8% of the human genome (9). Most of the identified elements are defective due to mutations and/or deletions within their genes, but some elements have conserved fully open reading frames (3, 12, 17, 18). A systematic search for coding envelope genes has led to the identification of 16 such genes (8). It had previously been demonstrated that one of these genes, the HERV-W envelope gene, is indeed functional, since it can induce cell-cell fusion when expressed in cells possessing the RDR receptors (5, 10) and since pseudotypes generated with human immunodeficiency virus type 1 (HIV-1) virions are infectious (2, 10). In this study, we show that another envelope protein, associated with an endogenous retrovirus of the so-called HERV-FRD family (see references 16 and 17) and sorted out by a systematic screen of the human genome for fusogenic envelopes (4), can also confer infectivity on pseudotypes generated with lentiviral virions. To do so, we cloned the HERV-FRD envelope gene into a human cytomegalovirus (hCMV) promoter-driven expression vector and used a previously described assay (10), as schematized in Fig. 1A. In this assay, human 293T cells are cotransfected with an expression vector for the retroviral proteins—except the envelope—from type C viruses (murine leukemia virus [MLV]) or lentiviruses (HIV-1 and simian immunodeficiency virus [SIV]), a corresponding lacZ gene-marked defective retroviral vector, and an expression vector for the env gene to be tested (or an empty vector as a negative control and a vector for the amphotropic MLV envelope as a positive control). Then, the pseudotype virions are assayed for infectivity after recovery of the transfected-cell supernatant 36 h posttransfection, transfer of the supernatant onto target test cells, and centrifugation of the plates (i.e., spinoculation [see reference 10]) and, after an additional 60-h period, counting the lacZ-positive test cell colonies by in situ histochemical staining for ß-galactosidase activity. The results of such an assay are given in Fig. 1B and Table 1, for the various viral cores (MLV, SIV, and HIV-1) and target cells tested, which included murine, feline, and human cells. It can be clearly observed that the HERV-FRD envelope, when expressed on lentiviral particles, confers infectivity on the virions, with evidence for viral titers on feline (G355-5) and human (TE671 and 293T) cells in the 100- to 500-CFU/ml range. Interestingly, the murine 3T3 cells and the human HeLa cells are refractory to infection (although they are positive for the control amphotropic MLV envelope), a result consistent with the absence of any detectable syncytium formation in a fusion assay carried out with these cells (see below). It can also be noted that the MLV core is much less efficient—if at all—than the lentiviral cores, as similarly observed for the HERV-W envelope (2, 5, 10). Finally, since some envelopes are sensitive to shearing, viral titers were also measured in the absence of the spinoculation step: in all cases, its omission (data not shown) resulted in a 5- to 20-fold reduction of the titers in Table 1 (and similarly for the simian FRD envelopes assayed as shown in Fig. 2).

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FIG. 1. Infection assays using retroviral particles pseudotyped with the HERV-FRD and control envelopes. (A) Rationale of the assay. To determine whether the HERV-FRD envelope could confer infectivity on MLV, HIV, or SIV Env-defective pseudoparticles, corresponding pseudotype viruses were produced by cotransfecting 7.5 x 105 293T cells with 0.55 µg of the phCMV-HERV-FRD vector expressing the HERV-FRD envelope (or pcDNA3 for the negative control and phCMVampho for the amphotropic MLV envelope positive control); 1.75 µg of a vector encoding the retroviral proteins (except the envelope) of MLV (phCMVintron) (13), HIV (pCMVR8.91) (20), or SIV (pSIV3+) (13); and 1.75 µg of the corresponding defective retroviral vectors marked with a ß-galactosidase reporter gene (pMFGsnlslacZ [7], pHR'CMVlacZ [20], and R9SA [14]), by calcium phosphate precipitation (Invitrogen). At 36 h posttransfection, viral supernatants were collected and filtered through 0.45-µm-pore-size membranes. Target cells (murine 3T3; feline G355-5; or human TE671, HeLa, and 293T cells) were seeded in 24-well plates at a density of 104 cells per well and incubated overnight at 37°C. Five to 500 µl of virus samples containing 4 µg of Polybrene per ml was added to the cells and centrifuged for spinoculation at 1,200 x g for 2 h 30 min at 25°C (10). After removal of the supernatants, the cells were incubated in regular medium for 60 h at 37°C. Viral titers were then measured by X-Gal (5-bromo-4-chloro-3-indolylphosphate) staining of the cells and expressed as lacZ CFU per milliliter of viral supernatant. (B) Infectivity of SIV-based retroviral particles pseudotyped with the HERV-FRD envelope (or with no protein in the "SIV-None" control), as assayed using human 293T cells as target cells and X-Gal staining, following the protocol described for panel A.

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TABLE 1. Infection assay of the HERV-FRD envelopea

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FIG. 2. Infection and cell-cell fusion activities and host range of the HERV-FRD and simian orthologous envelopes. Upper panel: infection of feline (G355-5), human (293T and HeLa), and murine (3T3) cells by SIV-based particles pseudotyped with the indicated ERV-FRD envelopes. Experimental conditions were the same as in Fig. 1, with negative and positive control values as in Table 1. NWM, New World monkey (Callithrix jacchus marmoset). Lower panel: cell-cell fusion assay for the ERV-FRD envelopes and cells as in the upper panel. The G355-5 and HeLa cells were transfected using Lipofectamine (Invitrogen; 2 µg of DNA for 5 x 105 cells), and the 293T and TE671 cells were transfected using calcium phosphate precipitation (Invitrogen; 5 µg of DNA for 5 x 105 cells). Fusion activities were measured 12 to 36 h posttransfection of the corresponding expression vectors. To visualize syncytia, cells were fixed in methanol and stained by adding May-Grünwald and Giemsa solutions (Sigma) according to the manufacturer's instructions. The fusion index, which represents the percentage of fusion events in a cell population, is defined as [(N - S)/T] x 100, where N is the number of nuclei in the syncytia, S is the number of syncytia, and T is the total number of nuclei counted.

Previous experiments had shown that HERV-FRD is a very ancient family, which entered the primate branch after the prosimian and simian divergence but before the split between New World and Old World monkeys, 40 million years ago (4). Moreover, we had identified in the simian genomes—by PCR using primers in the HERV-FRD provirus flanking DNA—the gene orthologous to the human envFRD gene and shown that it is present in a fully coding state in all simians, from New World monkeys to humans (4). We therefore assayed whether the simian orthologous env genes also confer infectivity on SIV-pseudotype virions. The results of an experiment similar to that carried out for Fig. 1 for the human gene are given in Fig. 2, which discloses the following features: (i) all simian ERV-FRD envelopes (with one exception [see below]) confer infectivity on the pseudotype virions, with closely related viral titers (up to 2,000 CFU/ml) and target cell specificity, thus demonstrating conservation over >40 million years of the ERV-FRD envelope function, but (ii) there is one exception to this rule for the gibbon envelope, which is negative for all the target cells tested. Interestingly, this negative result is not due to a severe defect in the gibbon envelope, since the protein is still capable of promoting cell-cell fusion, as illustrated in the lower panel of the figure by an experiment carried out in parallel using the same expression vectors and cells: the results show that for all simian envelopes (except the gibbon envelope) there is a good correlation, as expected, between infectivity and cell-cell fusion and that the gibbon envelope is clearly positive for cell-cell fusion, with the same cell specificity as that of the other simian envelopes. These features, which de facto uncouple the processes of cell-cell fusion and viral entry, could be accounted for by either intrinsic differences in the molecular requirements for the two processes or differences, possibly, in the envelope viral load and/or stability (see discussion in references 1, 10, and 11). At the sequence level, it is noteworthy that the gibbon envelope discloses strong conservation with the other simian FRD envelopes, as it is 97% identical to the human sequence, i.e., it is closer than the infection-positive marmoset New World monkey envelope, which is only 88% identical to the human sequence (Fig. 3A). Actually, comparison of the gibbon and human sequences reveals only four mutations which are gibbon specific, two within the SU and two within the TM subunits (shaded in Fig. 3A). Preliminary experiments using a chimeric construct with the human SU domain fused to the gibbon TM moiety strongly suggested that mutations in the TM subunit were responsible for the loss of infectivity of the gibbon envelope, as infectivity of the chimeric envelope remained low (data not shown). Two constructs with reversion of the identified TM point mutations were therefore tested for infectivity, one with the F-to-L reversion at position 490 and one with the T-to-N reversion at position 532. As illustrated in Fig. 3B, the T-to-N reversion fully restored infectivity to the gibbon envelope, with no effect of the F-to-L mutation located in the hydrophobic transmembrane domain of the TM subunit. Due to its specific location in the cytoplasmic tail of the envelope protein, it is likely that the N-to-T mutation alters some of the functions and interactions associated, in infectious retroviruses, with this specific domain and involved in the loading, stability, and conformational transition of the Env proteins (e.g., see references 6, 15, and 19; see also references in reference 1). In this respect, the gibbon envelope together with the other currently characterized simian envelopes can be considered natural mutants and be of use in studying the refined molecular processes associated with envelope function and virus entry.

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FIG. 3. Comparison of the simian ERV-FRD envelopes and identification of a unique point mutation responsible for the gibbon envelope loss of function. (A) Aligned primary sequences of the HERV-FRD and simian orthologous envelopes. Abbreviations: Hu, Homo sapiens; Cpz, chimpanzee; Go, gorilla; Oo, orangutan; Gib, gibbon; Mcq, macaque; Nwm, New World monkey. Accession numbers are AL136139 for the human gene and AJ577595 to AJ577600 for the simian genes. The RVRR canonical motif for SU-TM cleavage is highlighted, and the predicted transmembrane hydrophobic domain is boxed. Amino acids differing between the human and gibbon proteins are indicated with an asterisk, among which those unique to the gibbonsequence are shaded. The two amino acids in the TM moiety of the gibbon protein that were mutated and assayed for reversion to the infectious phenotype are indicated with an arrow. (B) Infection of feline (G355-5) and human (293T) cells by SIV-based particles pseudotyped with the wild-type (wt) and mutant (F490L or T532N) gibbon FRD envelopes, with the human FRD envelope as a control. The indicated amino acid changes in the gibbon envelope were performed by a three-fragment ligation, using PCR fragments generated with oligonucleotides containing the gibbon-to-human single-base mutations (TTTCTT and AATACT for amino acid positions 490 and 532, respectively), and subsequent sequencing of the resulting constructs. Infection assays were performed as described for Fig. 1. The viral titers are averages from two independent experiments.

Whatever the underlying molecular events, the present functional characterization of the ERV-FRD envelopes is strongly suggestive of an ancestral infection event by a functional retrovirus and its subsequent endogenization, with some of the envelope functions retained in evolution. Close examination of the primate FRD envelope sequences indeed reveals a prevalence of synonymous rather than nonsynonymous substitutions (ratios for all pairwise comparisons, >3, by the SNAP.pl program at the www.hiv.lanl.gov site), thus suggesting positive selection for a physiological function which remains to be unraveled.

 
This work was supported by the CNRS and by grants from the Ligue Nationale contre Le Cancer (Equipe Labelisée).

 
* Corresponding author. Mailing address: Unité des Rétrovirus Endogènes et Eléments Rétroïdes des Eucaryotes Supérieurs, UMR 8122 CNRS, Institut Gustave Roussy, 94805 Villejuif, France. Phone: 33/1-42-11-49-70. Fax: 33/1-42-11-53-42. E-mail: heidmann{at}igr.fr.

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Journal of Virology, January 2004, p. 1050-1054, Vol. 78, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.2.1050-1054.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blaise, S. Articles by Heidmann, T. Search for Related Content PubMed PubMed Citation Articles by Blaise, S. Articles by Heidmann, T.