INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

Controls of the rate of mRNA decay play an important role in mammalian gene expression (25, 37). Different mRNAs have characteristic half-lives that range from less than 15 min to more than 10 h. The decay rates of some mRNAs are dependent upon the stage of the cell cycle or are responsive to external stimuli such as exposure of the cells to hormones or growth factors (3, 6). While much has been learned concerning mRNA degradation pathways in yeast (39, 43), considerably less is known about the decay mechanisms in mammalian cells. Although a number of mammalian RNases have been isolated, it is unclear which enzymes are components of the mRNA degradation machinery, how they are targeted to mRNAs, or how their activities are controlled (1, 2). In addition, the regulated degradation of many mRNAs depends upon their being translated; yet the mechanism of this linkage is unclear (14). In this context, the virion host shutoff (Vhs) protein of herpes simplex virus (HSV) provides an attractive model system for studying mechanisms of mammalian mRNA decay.

Vhs is a 58-kDa polypeptide encoded by the viral UL41 open reading frame, which is a minor structural component of HSV virions (20, 33, 40). During lytic infections, copies of the Vhs (UL41) polypeptide, which enter the cell as components of infecting virions, accelerate the degradation of cellular mRNAs, contributing to an overall decrease in host protein synthesis (11, 38). Following the onset of viral transcription, Vhs also accelerates the turnover of viral mRNAs (19, 27, 28, 42). By shortening the half-lives of all mRNAs, it helps redirect the cell from host to viral gene expression, and facilitates the sequential expression of different classes of viral genes (31).

A number of studies suggest that Vhs (UL41) is itself an endonuclease. The UL41 homologues from HSV and other alpha herpesviruses share sequence homologies with a family of mammalian, Saccharomyces cerevisiae, bacterial, and phage nucleases (5, 9; D. N. Everly, Jr., P. Feng, I. S. Mian, and G. S. Read, unpublished data). For several of these homologues, point mutations that alter key conserved residues abrogate the nuclease activity, and mutations that alter the corresponding residues of Vhs inactivate its ability to induce mRNA decay (Everly et al., unpublished). Extracts of detergent-solubilized virions contain an endonuclease activity that is Vhs dependent since it is found in wild-type but not Vhs mutant virions and can be inhibited by Vhs-specific antisera (44). Furthermore, rabbit reticulocyte lysates containing in vitro-translated Vhs contain an endonuclease activity (7, 44). Although these studies are suggestive, the virion preparations that exhibited nuclease activity probably contained a number of contaminating cellular proteins, while the reticulocyte lysates contained the full complement of cytoplasmic proteins. Thus, one cannot exclude the possibility that Vhs activates a cellular endonuclease but is not itself a nuclease.

Although Vhs does not discriminate between mRNAs, it does exhibit two important kinds of selectivity. First, it exhibits a strong preference for mRNAs, as opposed to non-mRNAs. This is true in vivo (27, 28) as well as in in vitro decay reactions containing either cytoplasmic extracts from infected cells (18, 41) or in vitro-translated Vhs (44). Second, recent studies suggest that Vhs does not cleave mRNAs at random sites but may initiate degradation near regions of translation initiation. In infected cells, sequences near the 5' end of an mRNA are degraded prior to those near the 3' end of the transcript (17), while in rabbit reticulocyte lysates, in vitro-translated Vhs initially targets sites near the 5' end of an mRNA (7). In addition, in vitro-translated Vhs preferentially induces cleavage at sites downstream from a picornavirus internal ribosome entry site (IRES) (8, 23). In contrast, the Vhs activity in solubilized virions is not restricted to mRNAs and cleaves target RNAs at multiple sites throughout the molecule (44). These data suggest that, in the absence of key cellular factors, the Vhs-dependent endonuclease is significantly less specific than in intact cells. To explain this discrepancy, we searched for cellular proteins that interact with Vhs using the yeast two-hybrid system and coimmunoprecipitation assays. The results show that Vhs interacts with the cellular translation initiation factor eIF4H and suggest a mechanism for targeting Vhs to mRNAs and to regions of translation initiation, as well as for linking mRNA decay and translation.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Cells and virus. Vero cells and HSV type 1 (HSV-1), strain KOS, were grown as described previously (29). The recombinant vaccinia virus vvT7 expresses the T7 RNA polymerase (26) and was obtained from Lindsey Hutt-Fletcher (University of MissouriKansas City).

Antibodies. A polyclonal rabbit antiserum raised against a Vhs-LacZ fusion protein has been described previously (33). Monoclonal antibody that reacts with an epitope of the influenza virus hemagglutinin (HA) was purchased from Covance.

Plasmids. (i) Plasmids containing wild-type and mutant Vhs alleles. The plasmid pKOSamp contains the Vhs (UL41) open reading frame from wild-type HSV-1 (KOS) cloned into the vector pcDNA1.1 A (Invitrogen) (10). Various mutant Vhs alleles have been constructed by site-directed mutagenesis of pKOSamp. Each of the alleles D34N, D82N, E192Q, D194N, D195N, T211S, T211A, D213N, D215N, and D261N contains a single amino acid change in the Vhs allele of HSV-1 (KOS). The name of each allele includes the number of the residue that is altered, preceded by the wild-type amino acid and followed by the amino acid to which it is changed. For example, in D194N an aspartic acid at residue 194 is changed to asparagine. Each mutation alters an amino acid that is conserved in Vhs and a number of cellular nucleases and that has been shown to be important to the activity of some of those cellular enzymes. Each mutation also abrogates the mRNA degradative activity of the Vhs allele following transfection of mammalian cells. Construction and characterization of these alleles will be described elsewhere (Everly et al., unpublished). Construction of R435H, in which arginine 435 is changed to histidine, has been described previously (10). The mutation in the allele referred to in this work as T214I is that carried by the mutant virus Vhs1. This mutant lacks detectable Vhs activity and has been characterized extensively in previous studies (9, 18, 28, 29, 32). The mutants K(89-489) and K(1-453) encode Vhs polypeptides lacking the first 88 or last 36 amino acids of the wild-type polypeptide, respectively. The structure of each mutant allele was confirmed by DNA sequencing.

(ii) Plasmids for yeast two-hybrid interactions. The plasmid pAS2-Vhs contains the UL41 open reading frame of HSV-1 (KOS) inserted between the EcoRI and BamHI sites of pAS2-1 (Clontech). pAS2-Vhs encodes a fusion protein containing the Gal4 DNA binding domain (amino acids 1 through 147) followed by 16 amino acids encoded by the polylinker and the entire 489-amino-acid Vhs polypeptide. pAS2-Vhs was used to screen a commercially available library of HeLa cell cDNAs cloned into pACT2 (Clontech). pACT2-4H and pACT2-4H_i are two plasmids isolated from that library. To confirm the specificity of the two-hybrid interaction, the vectors used to express Vhs and eIF4H_i were switched. The two-hybrid assay then was repeated using pAS2-4H_i, which encodes a fusion protein of the Gal4 DNA binding domain and eIF4H_i, and pACT2-Vhs, which encodes a Gal4 activation domain-Vhs fusion protein.

(iii) Plasmids for GST pull-down experiments. For GST pull-down experiments, the eIF4H open reading frame was PCR amplified and cloned between the BamHI and SalI sites of pGEX-5-3 (Amersham-Pharmacia) to yield pGST-4H, which encodes a glutathione S-transferase (GST)-eIF4H fusion protein that can be expressed in bacteria. A similar procedure was used to construct pGST-4H_i, which encodes a fusion protein of GST and eIF4H_i.

(iv) Plasmids for transient expression of Vhs and eIF4H. For expression of an epitope-tagged version of eIF4H in mammalian cells, sequences encoding HA-tagged eIF4H were PCR amplified from pACT2-4H and inserted between the NcoI and SacI sites of pTM1, to yield pTM1-4H. Parallel procedures were used to construct pTM1-4H_i, encoding an HA-tagged eIF4H_i. To express Vhs, the UL41 open reading frame was PCR amplified and inserted between the NcoI and BamHI sites of pTM1, to yield pTM1-Vhs.

Yeast two-hybrid screen. A search for cellular cDNAs encoding Vhs-interacting proteins was performed using the Matchmaker Two-Hybrid System 2 (Clontech) according to standard techniques (12). Yeast strain Y190, which contains the his3 and lacZ genes driven by Gal4-responsive promoters, was transformed with pAS2-Vhs. Cells receiving the bait plasmid were transformed with plasmids from a commercially available library of HeLa cell cDNAs in the Gal4 activation domain vector pACT2 (Clontech). Cells that grew on synthetic dropout (SD) medium containing 35 mM 3-aminotriazole (3-AT) and lacking tryptophan, leucine, and histidine potentially did so because of activation of the Gal4-responsive his3 gene. These were replica plated onto filters and screened for lacZ expression according to standard protocols (Clontech). cDNA-containing plasmids were isolated from blue colonies, amplified in Escherichia coli strain KC8 (Clontech), and analyzed by restriction enzyme digestion. Selected plasmids were used to retransform yeast strain Y187 along with the pAS2-Vhs. Plasmids that activated lacZ expression in the presence of pAS2-Vhs, but not in the presence of the DNA binding domain vector pAS2-1 or an unrelated bait plasmid containing a cDNA for lamin C (Clontech), were judged to encode potential Vhs-interacting proteins. cDNA sequences were determined and compared to known DNA and protein sequences using the BLAST network service and sequence analysis software of the National Center for Biotechnology Information. Quantitative analyses of LacZ expression in yeast containing selected bait and cDNA-containing plasmids were performed according to published protocols (4).

Recombination-based two-hybrid assay. A recombination-based yeast two-hybrid assay was performed as described previously (30). Yeast strain Y190 was transformed with pACT2-4H and the large SmaI fragment of pAS2-Vhs (see Fig. 4). This fragment contains all of the pAS2-Vhs sequences except those encoding Vhs amino acids 148 through 343. Transformations also included an EcoRI-XbaI fragment from another plasmid containing the entire mutant Vhs allele whose binding to eIF4H was to be tested. Transformants were plated on SD medium containing 35 mM 3-AT and lacking tryptophan, leucine, and histidine to select for yeast that grew because transcription of the Gal4-responsive his3 gene had been activated. Within the yeast, recombination between the SmaI fragment of pAS2-Vhs and the fragment containing the mutant Vhs gene resulted in reconstitution of a pAS2-Vhs plasmid containing the Vhs mutation. Transformations that contained an EcoRI-XbaI fragment carrying the wild-type Vhs allele gave rise to 200 to 300 colonies on the selective medium which also expressed the lacZ gene from a Gal4-responsive promoter. In contrast, transformations that contained just pACT2-4H and the large SmaI fragment of pAS2-Vhs, but no EcoRI-XbaI fragment containing a Vhs allele, gave rise to at most 5 to 10 colonies on selective medium. Mutant Vhs alleles that gave rise to a number of colonies comparable to that of wild-type Vhs were judged to encode proteins that retained eIF4H binding activity. In contrast, several mutant alleles gave rise to no more than the background number of colonies and were judged to encode proteins that had lost the ability to interact with eIF4H. All of the mutants gave rise to a number of colonies comparable to that of wild-type Vhs or a number that was indistinguishable from the background.

In vitro transcription and translation. Vhs protein was produced by coupled in vitro transcription and translation using the TnT T7 Quick Coupled Transcription/Translation System from Promega. Translation reactions were adjusted to 50 mM Tris-HCl (pH 8.0)-100 mM NaCl-1% Nonidet P-40-1 mM EDTA and used in GST pull-down experiments.

Virus infection and preparation of infected cell extracts. Vero cells were infected with 5 PFU of HSV-1 (KOS)/cell (28). Sixteen hours later, the cells were harvested and lysed by resuspension at a concentration of 10⁷cells/ml in ice-cold lysis buffer (50 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1% NP-40, 1 mM EDTA) containing protease inhibitors (2 µg of aprotinin per ml and 50 µg of phenylmethylsulfonyl fluoride per ml). After 10 min on ice, the nuclei were pelleted, and the supernatant was saved for GST pull-down experiments.

GST pull-down assays. E. coli, strain BL21, was transformed with plasmids encoding fusion proteins of GST and wild-type or mutant forms of eIF4H. Overnight cultures were induced for 4 h with 0.1 mM isopropyl--D-thiogalactopyranoside (IPTG), after which the bacteria were harvested, resuspended in phosphate-buffered saline containing 1% (vol/vol) Triton X-100 and lysozyme (0.1 mg/ml), and lysed by sonication. Bacterial extracts were clarified and mixed with a 50% (vol/vol) slurry of glutathione-Sepharose 4B in phosphate-buffered saline (50 µl per 1 ml of lysate). After 15 min on ice, the beads were pelleted, washed, and resuspended in 0.4-ml portions of either cytoplasmic supernatant from HSV-infected cells or rabbit reticulocyte lysates containing in vitro-translated Vhs. Thirty minutes later the beads were pelleted and washed. Complexes of GST-eIF4H bound to Vhs were eluted with a small volume of 10 mM glutathione in 50 mM Tris (pH 8.0) and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). [³⁵S]methionine-labeled Vhs from in vitro translation reactions was detected by autoradiography of the dried gel, while unlabeled Vhs from lysates of infected cells was detected by Western blotting (33).

Coprecipitation of Vhs and eIF4H from mammalian cells. Vero cells were grown to 90% confluence in 100-mm-diameter petri dishes and infected with vaccinia virus vTF7-39 (5 PFU/cell) as described previously (21). Thirty minutes later, they were transfected with 5 µg of pTM1-Vhs and 5 µg of either pTM1-4H_i or a mixture of pTM1-4H_i and pTM1-4H. Twenty hours after transfection, the cells were harvested and lysed, and immunoprecipitates were prepared using either a Vhs-specific polyclonal antiserum or HA-specific monoclonal antibody (33). Precipitated proteins were resolved by SDS-PAGE and analyzed by Western blotting using the reciprocal antibody.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Two-hybrid screen for Vhs-interacting proteins. To identify cellular proteins that interact with Vhs, the entire Vhs (UL41) open reading frame of HSV-1 was cloned downstream from the Gal4 DNA binding domain and used as bait in a yeast two-hybrid screen of a HeLa cell cDNA library cloned downstream from the Gal4 activation domain. HeLa cells were chosen as the source of cellular cDNAs because they are readily infected with HSV-1 and are very susceptible to Vhs-induced mRNA degradation. Of 1.7 × 10⁷ clones that were screened, 90 were judged to be candidates for cDNAs encoding Vhs-interacting proteins since they induced simultaneous expression of his3 and lacZ genes from Gal4-responsive promoters. Analysis of all 90 cDNAs revealed that they fell into five groups on the basis of their size and restriction patterns. Selected cDNA-containing plasmids were amplified in E. coli and reintroduced into yeast, where, once again, they activated a Gal4-responsive promoter in the presence of a Vhs-containing bait plasmid. Transcription was not activated when the cDNAs were used in combination with the Gal4 DNA binding domain vector lacking Vhs or with an unrelated bait plasmid containing a lamin C cDNA. Interchanging the vectors in which Vhs and the cDNAs were expressed did not affect their ability to activate transcription of a Gal4-responsive promoter, further verifying the specificity of the two-hybrid interaction.

View larger version (93K): [in this window] [in a new window]   FIG. 1.   Sequences of eIF4H and two cellular Vhs binding proteins. The published sequence of eIF4H is shown, along with the deduced sequences of two Vhs binding proteins identified in the yeast two-hybrid screen. Amino acids that are identical to those of eIF4H are shaded grey. Asterisks indicate the absence of an amino acid and were introduced to facilitate alignment of eIF4H, Vhs binding protein 1, and Vhs binding protein 2.

In vitro interaction between Vhs and eIF4H. To corroborate the results of the two-hybrid assay, eIF4H_i was cloned into pGEX-5X-3 (Pharmacia) to produce pGST-4H_i, a plasmid encoding a fusion protein of GST and eIF4H_i. GST-eIF4H_i was produced in bacteria, bound to glutathione-Sepharose, and tested for its ability to interact with Vhs in solution. Two sources of Vhs were used: [³⁵S]methionine-labeled Vhs produced by in vitro transcription and translation in rabbit reticulocyte lysates and unlabeled Vhs from lysates of HSV-infected cells. Control reactions assayed the binding of Vhs to GST produced in bacteria that contained the pGEX-5X-3 vector. After incubation with Vhs, the beads were washed and the bound proteins were eluted with 10 mM glutathione. Bound proteins were analyzed by SDS-PAGE and autoradiography to detect ³⁵S-labeled Vhs (Fig. 2A) or by SDS-PAGE and Western blotting to detect Vhs from infected cell lysates (Fig. 2B). Vhs from both sources bound to beads containing GST-eIF4H_i, but not to beads charged with just GST, indicating a specific interaction between Vhs and eIF4H_i.

View larger version (35K): [in this window] [in a new window]   FIG. 2.   Binding of Vhs to a GST-eIF4Hi fusion protein in vitro. GST or a GST-eIF4Hi was bound to glutathione-Sepharose 4B and incubated with rabbit reticulocyte lysates containing in vitro-translated Vhs (A) or a cytoplasmic extract from HSV-1 infected cells (B). Bound proteins were eluted with 10 mM glutathione and analyzed by SDS -PAGE. In vitro-translated proteins were detected by autoradiography, while Vhs from infected cells was detected by Western blotting. In both panels, lane 1 contains the starting material, lane 2 contains proteins that bound to GST, and lane 3 contains proteins that bound to GST-eIF4Hi.

Coimmunoprecipitation of Vhs and eIF4H. To determine whether Vhs and eIF4H interact in vivo, coimmunoprecipitation experiments were performed in which HA-tagged forms of eIF4H and eIF4H_i were expressed in Vero cells in the presence and absence of Vhs. After lysis of the cells, immunoprecipitates were prepared using either Vhs-specific antisera or an HA-specific monoclonal antibody and examined for the presence of coprecipitated proteins by Western blotting using the reciprocal antibody. Previous studies have shown that small amounts of the Vhs protein are produced in cells transfected with the wild-type UL41 allele, while significantly more of the protein is expressed in cells transfected with inactive Vhs alleles (29). This is presumably due to the fact that active Vhs protein degrades its own mRNA, thereby reducing the amount of the protein that is produced in transfected cells. Consequently, to maximize the amount of the Vhs protein that was produced, we used a Vhs allele (D194N) with a single point mutation that changes an aspartic acid to asparagine at residue 194. Vhs shares a number of highly conserved residues with a family of mammalian, yeast, bacterial, and phage nucleases, among them aspartate 194. For several of these cellular homologues, structural studies and site-directed mutagenesis have implicated aspartate 194 as essential for nuclease activity. Therefore, the D194N allele was constructed in anticipation that it would encode a Vhs protein that lacks nuclease activity and would be unable to induce mRNA decay in transfected cells. Recent studies have proven this to be the case (Everly et al., unpublished). In addition, experiments (see Fig. 5) show that the D194N Vhs protein retains the ability to interact with eIF4H in the yeast two-hybrid system and in GST pull-down assays.

View larger version (26K): [in this window] [in a new window]   FIG. 3.   Coimmunoprecipitation of Vhs and epitope-tagged eIF4H from mammalian cells. (A and B) Vero cells were infected with the recombinant vaccinia virus vTF7-39 and transfected with plasmids expressing HA-tagged eIF4Hi (lanes 1); Vhs (lanes 2); HA-tagged eIF4Hi and Vhs (lanes 3); or HA-tagged eIF4Hi, HA-tagged eIF4H, and Vhs (lane 4, panel A). Cell extracts were prepared and immunoprecipitated (upper gels) using Vhs-specific antiserum (A) or HA-specific monoclonal antibody (B). Immunoprecipitates (IP) were analyzed for coprecipitated proteins by Western blotting (upper gels) using the reciprocal antibodyeither the HA-specific monoclonal antibody (A) or Vhs-specific antiserum (B). To check for protein expression, an aliquot of the cell lysate was analyzed directly by Western blotting (lower gels) using HA-specific monoclonal antibody (A) or Vhs-specific antiserum (B).

Some inactive Vhs mutants fail to bind eIF4H. If this interaction between Vhs and eIF4H is important for Vhs activity, one would expect that some mutant Vhs polypeptides, which do not induce mRNA decay, would also fail to bind eIF4H. To test this prediction, we screened a panel of Vhs mutants for the ability to interact with eIF4H using the conventional two-hybrid assay or a recombination-based two-hybrid system as diagrammed in Fig. 4. Each of the mutants is unable to stimulate mRNA decay, either during a virus infection or in transfected cells (10, 29, 32; Everly et al., unpublished). The mutant proteins were also tested for binding of GST-eIF4H in a GST pull-down assay. All of the assays yielded similar results (Fig. 5).

View larger version (23K): [in this window] [in a new window]   FIG. 4.   Strategy of the recombination-based yeast two-hybrid assay. Yeast strain Y190 was transformed with pACT2-4H and the large SmaI fragment of pAS2-Vhs. Transformations also included an EcoRI-XbaI fragment from another plasmid containing the entire mutant Vhs allele whose binding to eIF4H was to be tested. Within the yeast, recombination between the SmaI fragment of pAS2-Vhs and the fragment containing the mutant Vhs gene resulted in reconstitution of a pAS2-Vhs plasmid containing the Vhs mutation. Transformants were plated on SD medium containing 35 mM 3-AT and lacking tryptophan, leucine, and histidine to select for yeast that grew because transcription of the Gal4-responsive his3 gene had been activated. Transformations that contained a Vhs allele encoding a protein that bound eIF4H gave rise to 200 to 300 colonies on the selective medium, while control transformations containing just pACT2-4H and the large SmaI fragment of pAS2-Vhs gave rise to at most 5 to 10 colonies.

View larger version (30K): [in this window] [in a new window]   FIG. 5.   eIF4H binding by wild-type and mutant Vhs. (A) The structures of the wild-type Vhs protein and various mutants. For deletion mutants, the Vhs residues included in the mutant protein are indicated. For each point mutant, the location of the altered residue is indicated by the vertical line above the bar representing the protein. The in vivo mRNA-degradative activity of each Vhs protein is shown in the column immediately to the right of the diagram. This was assayed in transfected Vero cells for all of the alleles and during virus infections for wild-type Vhs and the mutant T214I. The right-hand column indicates whether a Vhs protein binds (++) or does not () bind eIF4H in the conventional (superscript letter a) or recombination-based (superscript letter b) yeast two-hybrid system. (B) GST pull-down assays. Wild-type and mutant Vhs polypeptides were produced by in vitro translation and analyzed for the ability to bind a GST-eIF4H fusion protein as described for Fig. 2. Material that bound to GST-eIF4H and was eluted with 10 mM glutathione is shown in the upper gels (GST Pulldown). Aliquots of the total in vitro-translated material are shown in the lower gels (Input).

eIF4H mutants that fail to bind Vhs. To investigate the domain of eIF4H required for Vhs binding, a series of eIF4H deletion mutants were tested for their ability to interact with Vhs in the yeast two-hybrid and GST pull-down assays (Fig. 6). Shortening eIF4H from the carboxyl terminus to 191 amino acids reduced its ability to bind Vhs only marginally. Further shortening to 141 amino acids reduced binding to Vhs somewhat; although a strong interaction still remained. However, truncation of eIF4H to 104 amino acids abolished detectable binding to Vhs, suggesting that residues between amino acids 105 and 141 are important for the interaction. Consistent with this, deletion of amino acids 90 to 150 abolished the interaction. Deletion of amino acids 1 through 89 reduced binding to Vhs only a few fold, indicating that the first 89 amino acids of eIF4H do not contribute significantly to the interaction. Finally, a fragment containing amino acids 90 through 150 of eIF4H_i resulted in almost as strong a two-hybrid interaction as did full-length eIF4H_i. Amino acids 138 through 150 of this fragment are present in eIF4H_i but not in eIF4H. Since Vhs interacts with both eIF4H and eIF4H_i, a fragment containing amino acids 90 through 137 of eIF4H is sufficient to allow strong interaction with Vhs.

View larger version (42K): [in this window] [in a new window]   FIG. 6.   Vhs binding by eIF4H mutants. (A) The structures of eIF4H mutants are shown, with residues shared by eIF4H and eIF4Hi shaded light grey and those unique to eIF4Hi shaded dark grey. At the right is the relative binding of each eIF4H protein to Vhs in the conventional yeast two-hybrid assay. Binding is expressed as the amount of lacZ activity resulting from the activation of a GAL4-dependent lacZ gene, where the activity induced by eIF4Hi is defined as 100%. (B) Wild-type Vhs was produced by in vitro translation and analyzed for the ability to bind various fusion proteins containing GST fused to mutant forms of eIF4H. Vhs bound by the various GST-eIF4H polypeptides and eluted with 10 mM glutathione is shown in the upper gel (GST Pulldown). A Coomassie-stained gel of the mutant GST-eIF4H proteins is shown below (Input).

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

These studies demonstrate that the Vhs protein of HSV interacts with the cellular translation initiation factor eIF4H in vitro, in yeast, and in mammalian cells. The biologic importance of this interaction is suggested by the observation that several point mutations in Vhs, which abrogate its ability to induce mRNA decay, also abolish its ability to bind eIF4H.

Although in vivo Vhs is targeted to mRNAs and perhaps to regions of translation initiation, the Vhs-dependent nuclease that is observed in extracts of partially purified virions is much less specific (44). Our observation that Vhs binds eIF4H suggests a mechanism for targeting the Vhs activity. eIF4H shares a region of sequence homology with eIF4B and appears to be functionally similar in that both proteins stimulate the RNA helicase of eIF4A, possibly by increasing its processivity (34-36). Along with eIF4E and eIF4G, eIF4A is a component of the tripartite cap binding complex eIF4F (13). Thus, eIF4H appears to act at an early stage of cap-dependent translation initiation to help unwind mRNA secondary structure and facilitate scanning by the small ribosomal subunit (15). The data suggest a model in which binding to eIF4H somehow targets Vhs to mRNAs, as opposed to non-mRNAs, and to regions of translation initiation (Fig. 7).

View larger version (17K): [in this window] [in a new window]   FIG. 7.   Model for Vhs targeting. eIF4H (4H) stimulates the RNA helicase and RNA-dependent ATPase activities of eIF4A (4A). eIF4A, in turn, is a component of the tripartite cap-binding complex eIF4F. The dashed arrow depicts the functional, and perhaps physical, interaction of eIF4H with eIF4A. Vhs is targeted to mRNAs, as opposed to non-mRNAs, and to regions of translation initiation through its interaction with eIF4H.

While this model is attractive in outline, many of its details remain to be developed and tested. Although the model is based upon known functions of eIF4H and other cellular translation factors, it remains to be proven that binding to eIF4H actually leads to the localization of Vhs near regions of translation initiation. An implication of the model is that targeting of Vhs may be dependent not only on its interaction with eIF4H but also on a series of protein-protein and protein-RNA interactions involving other translation factors. Thus, Vhs binds eIF4H, which in turn interacts functionally, and perhaps physically, with eIF4A. eIF4A binds eIF4G, which interacts with eIF4E, which binds the mRNA cap. Whether all or only some of these interactions are required for efficient targeting of Vhs, or whether other protein-protein or protein-RNA interactions are also required, remains to be determined. In addition, it is interesting to speculate whether the Vhs-eIF4H interaction inhibits the normal activity of eIF4H. If this is the case, Vhs may affect gene expression both by degrading mRNA and by inhibiting the rate of translation initiation.

These results also are significant because of the light they shed upon mechanisms of eukaryotic mRNA decay in general. In eukaryotes, the regulated degradation of many mRNAs is dependent upon their being translated (14, 39). The observation that a viral mRNA degradation protein binds a cellular translation initiation factor provides a novel example of one way in which this linkage between mRNA decay and translation can be accomplished.

Although it is clear that Vhs induces mRNA turnover, it is uncertain whether it is itself a nuclease or, instead, somehow activates a cellular enzyme. Data suggesting that Vhs is an RNase include the observation that the Vhs homologues of alpha herpesviruses share sequence homologies and a number of key conserved residues with a family of mammalian, yeast, bacterial, and phage nucleases. For several of these nucleases, point mutations that alter key conserved residues abrogate the nuclease activity, and mutations that alter the corresponding residues of Vhs inactivate its ability to induce mRNA decay (Everly et al., unpublished). While these results are suggestive, the Vhs protein has not been purified and definitively proven to have nuclease activity. However, recent progress has been made in this area by taking advantage of the interaction between Vhs and eIF4H. In our laboratory, initial attempts to express Vhs in bacteria resulted in the production of Vhs protein that was insoluble, except in buffers containing high concentrations of guanidine hydrochloride or urea. However, coexpression of Vhs and a GST-eIF4H fusion protein resulted in the formation of soluble complexes of Vhs-GST-eIF4H that could be isolated by binding to glutathione-Sepharose and subsequent ion exchange chromatography. Complexes containing the wild-type Vhs protein were found to have RNase activity, while complexes containing either of two mutant forms of Vhs, which lack mRNA-degradative activity in mammalian cells but still bind eIF4H, lacked detectable nuclease activity (Everly et al., unpublished). The results indicate that Vhs indeed is an RNase, either by itself or as a complex with eIF4H. Interestingly, Lu and coworkers recently reported that extracts from yeast that expressed wild-type Vhs lacked detectable RNase activity but that RNase activity was observed after the extracts were supplemented with rabbit reticulocyte lysates (22). This observation is consistent with the possibility that one or more mammalian factors, such as eIF4H, are required to activate the nuclease activity of Vhs. However, a number of alternative interpretations exist. Additional characterization of purified Vhs and Vhs-eIF4H complex are required.

At present it is unclear how many times the Vhs nuclease cleaves an mRNA. Cleavage at a single site near the 5' end would be sufficient to inhibit further cap-dependent translation, while cellular 5'-to-3' and 3'-to-5' exonucleases exist which, in theory, could degrade the resulting fragments. Alternatively, Vhs may cleave mRNAs multiple times at sites that are progressively closer to the 3' end. Data from Elgadi and coworkers suggest that this may occur in rabbit reticulocyte lysates containing in vitro-translated Vhs (7). Whether it occurs in vivo remains to be determined.

The present results suggest obvious models for the role of eIF4H in Vhs-mediated degradation of mRNAs that are undergoing cap-dependent translation. However, Vhs also has been shown to induce endonuclease cleavage of mRNAs downstream from a picornavirus IRES in vitro (8, 23). Whether eIF4H is required for Vhs-directed decay of IRES-containing mRNAs is unclear. At present, it is unknown whether eIF4H is required for IRES-dependent translation. However, with the notable exception of eIF4E, many of the initiation factors that are required for cap-dependent translation also are required for initiation from a picornavirus IRES (16). If eIF4H is involved in initiation from picornavirus IRESs, it may play a similar role in the Vhs-mediated degradation of these mRNAs and those undergoing cap-dependent translation. However, Lu and coworkers recently reported that a Vhs polypeptide containing the T214I point mutation induces a residual amount of IRES-directed endonuclease activity in the rabbit reticulocyte in vitro degradation system (23). Interestingly, this mutation greatly diminishes binding of Vhs to eIF4H (Fig. 5). One possibility is that the T214I polypeptide retains a residual amount of eIF4H binding activity, which is sufficient for the residual amount of IRES-directed cleavage that is observed in vitro. Alternatively, Vhs may recognize IRES elements directly or through a cellular factor other than eIF4H. These and other questions are under investigation.