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Journal of Virology, January 2001, p. 921-933, Vol. 75, No. 2
Department of Viral Vaccine Research,
Wyeth-Lederle Vaccines, Pearl River, New York 10965
Received 10 April 2000/Accepted 16 October 2000
The noncoding sequence of five Edmonston vaccine viruses (AIK-C,
Moraten, Rubeovax, Schwarz, and Zagreb) and those of a low-passage Edmonston wild-type (wt) measles virus have been determined and compared. Twenty-one nucleotide positions were identified at which Edmonston wt and one or more vaccine strains differed. The location of
some of these nucleotide substitutions suggests that they may influence
the efficiency of mRNA synthesis, processing, and translation, as well
as genome replication and encapsidation. Five nucleotide substitutions
were conserved in all of the vaccine strains. Two of these were in the
genomic 3'-terminal transcriptional control region and could affect RNA
synthesis or encapsidation. Three were found within the 5'-untranslated
region of the F mRNA, potentially altering translation control
sequences. The remaining vaccine virus base changes were found in one
to four vaccine strains. Their genomic localization suggests that some
may modify cis-acting regulatory domains, including the
Kozak consensus element of the P and M genes, the F gene-end signal,
and the F mRNA 5'-untranslated sequence.
Measles virus (MV) is a highly
contagious human pathogen best known as the cause of one of the
classical "rash" illnesses of children. Few escaped this acute
infection and disease prior to the development of the currently used
live attenuated virus vaccines. Severe, even fatal complications,
particularly involving the respiratory and central nervous systems,
were not uncommon even in industrialized countries. Unfortunately, in
many parts of the less-well-developed world, measles continues to be
the major cause of preventable childhood mortality (9, 15,
16).
MV is an enveloped RNA virus of the genus Morbillivirus,
family Paramyxoviridae (32). Like other members
of this family, its genome is nonsegmented and of negative sense. The
16-kb linear RNA contains six nonoverlapping cistrons
(3'-N-P-M-F-H-L-5') that encode eight known polypeptides
(16, 27). In addition to the protein coding regions,
nearly 11% of the 16-kb MV genome is composed of noncoding RNA.
Presumably, this relatively small viral genome has maintained a
significant noncoding nucleotide sequence content for its functionally
important cis-acting elements.
cis-acting regulatory sequences found in all viral genomes
are essential for the orderly progression of the viral life cycle. These elements serve to specify, organize, and control gene expression and genome replication, often exerting these effects through
interaction with virus-encoded proteins or host cell factors. The
important role of cis-acting sequence elements is well
illustrated by the complex transcriptional regulatory schemes employed
by large DNA viruses such as herpes simplex virus or adenovirus
(38, 42). It is equally clear that cis-acting
regulatory elements are exploited by the smaller genomes of
negative-strand RNA viruses to control their replicative strategy
(27). In MV, like other paramyxoviruses, cis-acting sequences have been identified that have roles in
genome replication, genome packaging, translation, and mRNA synthesis, processing, and editing (2, 16, 19, 27, 41).
The known or proposed cis-acting signals in the MV genome
are summarized in Fig. 1 (2, 16,
19, 27, 41). The noncoding 107 nucleotides at the 3' end of the
negative-strand genome (called the leader transcriptional control
region [TCR]) include promoter sequences that initiate two distinct
RNA synthesis pathways: (i) production of an end-to-end copy of the
genome to generate the positive-strand replication intermediate and
(ii) an elongation-termination-reinitiation transcription pathway that
produces mRNAs corresponding to the six cistrons. Transcription
termination and reinitiation during mRNA synthesis is mediated by
conserved sequence elements located in each intergenic region: a
gene-end (GE) plus a gene-start (GS) signal separated by the
characteristic GAA nucleotide triplet forms the GE/GS signal. The
highly conserved GAA triplet is found between all intergenic GE and GS
signals except between the H and L genes, where a GCA triplet is found.
In addition to guiding transcription termination and reinitiation, the
GE/GS signal also directs mRNA polyadenylation.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.2.921-933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Analysis of the Noncoding Regions of Measles Virus
Strains in the Edmonston Vaccine Lineage
ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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FIG. 1.
MV genome map. The location of protein coding regions
(white boxes: N, P, V, C, M, F, H, and L) and noncoding regions
including the leader and trailer (black terminal boxes) and intergenic
regions (shaded blue) are shown, along with specialized sequence motifs
(2, 16, 19, 27, 41, 52). The TCRs at the 3' end of the
genomic RNA (leader TCR, nucleotides 1 to 107) and the 3' end of the
antigenomic RNA (trailer TCR, nucleotides 15785 to 15894) are enlarged
below the genome map. Genomic RNA synthesis initiation is shown as open
arrowheads in the enlargement of the leader and trailer TCR regions.
Sequence motifs shown in the expanded TCR maps include the leader,
trailer, GE and GS signals, the 16-base conserved terminal sequence
(shown 3' to 5'), the repeated B- and B'-box motif, the G(N)5 motif,
the ATG codon for the N gene adjacent to the leader, and the L protein
stop codon outside of the 5' end of the trailer. The positions of GE/GS
signals are designated with a combination of solid arrowhead (GS) and a
black box (GE). The leader TCR GE/GS symbol lacks a GE box, and the
trailer TCR GE/GS lacks a GS arrowhead to signify that these sequences
may not function identically to GE/GS elements in the intergenic
regions. The mRNA editing site is indicated below the V protein coding
region (6). Part of the F gene mRNA 5'-untranslated
nucleotide sequence is shown to illustrate the three in-frame AUG
initiator codons (5). At the bottom of the figure, several
symbols found in the TCR maps are defined.
cis-acting sequences similar to those in the leader TCR are also located in the 3'-terminal 109 nucleotides of the positive-stranded antigenome (the trailer TCR; Fig. 1). The trailer TCR exclusively directs initiation of negative-strand genome synthesis. Important sequence motifs found in the leader and trailer TCRs (Fig. 1) include the terminal 16 nucleotides that are thought to be part of the primary site of RNA polymerase recognition (20, 28), the G(N)5 (52) and B-box motifs (2) that have been implicated as regulators of MV transcription and replication, and a potential GS signal in the leader TCR prior to the N gene and a GE signal in the trailer TCR that terminates L gene mRNA synthesis (16, 19, 27, 41).
Two additional functional cis-acting sequences have been identified in the MV genome. One is the RNA editing site in the P cistron that permits addition of a G residue in some P mRNAs, resulting in the translational frameshift that directs synthesis of V protein (6). The second cis-acting sequence is found within the rather long noncoding intergenic region between the M and F genes. Part of this region specifies the nontranslated leader sequence of the F gene mRNA which has been shown to be an important determinant of translational efficiency and AUG codon selection (5, 13). Deletion of this translational control element from recombinant viruses also appears to compromise replication in vivo (55). Identification of yet other cis-acting elements is likely to occur as more of the MV noncoding sequences are scrutinized with transient assay systems and recombinant viruses.
cis-acting elements can be an important determinant of virus attenuation. For example, mutations in the leader sequence of human parainfluenza virus type 3 (PIV3) vaccine candidates have been shown to specify the temperature sensitivity, cold adaptation, and attenuation phenotypes (46). Some attenuated respiratory syncytial virus A2 (RSV A2) strains contain a nucleotide change in the GS signal of the M2 gene that contributes to their temperature-sensitive and attenuated phenotypes (56). Also, a GE signal mutation in the M gene of an attenuated RSV B strain has been found to compromise expression of the downstream SH gene (D. A. Buonagurio, personal communication). Similarly, a cis-acting sequence mutation has been noted within the GE signal of the mumps vaccine virus F gene that appears to also disrupt normal expression of the downstream SH gene (51). Taken together, these studies indicate that specific alterations of noncoding cis-acting sequence elements of negative-strand RNA viruses can modulate virus virulence and attenuation.
Genomic modifications that attenuate MV have only recently begun to be defined. For example, several studies have shown that viruses defective for V or C protein expression display attenuated growth characteristics in some model systems (12, 31, 34, 53, 55). Additionally, mutations in genes encoding C, V, P, and L proteins have been associated with reduced viral replication in the B95 lymphocyte cell line (50). Although these studies have started to define roles for various proteins in MV attenuation, the potential role of cis-acting sequences has so far received less attention. In one case, deletion of most of the F gene mRNA 5'-untranslated region from a recombinant MV strain did result in less-efficient replication in human thymus-liver implants engrafted into SCID mice (55), demonstrating that modification of this cis-acting sequence can modulate attenuation.
To learn more about the potential role of noncoding cis-acting sequences in MV attenuation, a comparative sequence analysis was performed on viruses in the Edmonston vaccine lineage. This study was based on posing two relatively simple questions. (i) Do the noncoding sequences from several optimally attenuated vaccine viruses differ from the Edmonston wt progenitor strain or an underattenuated Edmonston vaccine strain? (ii) If differences exist, do they affect noncoding sequences that may function as cis-acting sequences controlling gene expression or replication? The Edmonston virus lineage (39) is attractive for this type of comparative study for a variety of reasons. Six viruses from the vaccine lineage are available for comparison, including five independently generated vaccine strains and a low-passage laboratory isolate of Edmonston wt (10, 11, 16, 18, 21, 26, 30, 39). This provides a unique opportunity to examine the molecular consequences of similar but independent vaccine derivation schemes (39). Comparison of Edmonston wt and five different vaccine strains also provides an opportunity to examine the diversity of molecular mechanisms by which the attenuated phenotype is produced by different genotypes. Furthermore, the vaccine strains differ in the level of attenuation. Four of the five vaccines are adequately attenuated, while Rubeovax proved to be reactogenic (26), and this is an important point for comparison that should provide insight into what genome changes influence the degree of attenuation. Finally, transient expression systems and cDNA rescue technologies provide experimental systems to further analyze the genetic changes identified by sequence analysis (35).
To identify potential attenuation determinants within MV cis-acting regulatory elements we have sequenced the noncoding regions of a low-passage isolate of Edmonston wt and five vaccine derivatives. Base changes were identified at 21 noncoding positions when the vaccine genomes were compared to Edmonston wt. Five nucleotide substitutions were common to all vaccine strains, while the remaining substitutions were present in one to four vaccine viruses. The sequence comparison also revealed that Moraten and Schwarz contained identical noncoding region sequences and differed at five nucleotide positions from closely related Rubeovax.
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MATERIALS AND METHODS |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Cells, virus, and genome sequencing. Cell culture and MV propagation was performed as described in an accompanying article (33). The Edmonston wt isolate (11, 16) was a gift from Judy Beeler (Center for Biologics Evaluation and Research). Edmonston B (Rubeovax) (10, 26), AIK-C (30), Schwarz (Rimevax; SmithKline Beecham) (26, 40), and Zagreb (21) were generously provided by William Bellini and Paul Rota (Centers for Disease Control) (39). Moraten (Attenuvax; Merck & Co) (18) and a second preparation of Schwarz (Rimevax, SmithKline Beecham) (26, 40) were obtained from commercially available vaccine preparations. The two separate sources of Schwarz were analyzed independently.
Viral genome sequence was determined directly from DNA fragments generated by reverse transcription-PCR (RT-PCR) of RNA extracted from infected Vero cells (33). Cycle sequencing (17, 25) was performed using dye-labeled terminators and Taq DNA polymerase (Applied Biosystems), followed by analysis on an ABI Prism automated sequence apparatus. Primers used for PCR amplification and sequencing on both cDNA strands were designed based on published MV sequences (GenBank accession numbers K01711 and S58435). Data analysis was performed using the MacVector (Oxford Molecular Group) and Lasergene (DNAstar, Inc.) software packages. The MV sequences have been deposited in GenBank (Edmonston wt, AF266288; AIK-C, AF266286; Moraten AF266287; Rubeovax, AF266289; Schwarz, AF266291; Zagreb, AF266290).| |
RESULTS |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Comparative sequence analysis. Evidence that the base changes within the cis-acting elements of the MV genome may contribute to viral attenuation was sought through comparative analysis of the noncoding region nucleotide sequence of five Edmonston vaccine viruses and a low-passage Edmonston wt isolate. Potentially attenuating cis-acting nucleotide substitutions were indeed located, providing the framework for design of future genetic studies aimed at dissecting the molecular basis of attenuation using the MV minireplicon and recombinant MV systems.
Interpreting such comparative sequence analyses requires caution given the lack of the true Edmonston wt progenitor virus. Fortunately, a low-passage derivative of the Edmonston clinical isolate was available for these studies. This virus was passaged 13 times (39) prior to the analysis presented here, so it is possible that some degree of tissue culture adaptation may be reflected in the Edmonston wt sequence. Nevertheless, it is the best approximation of the original clinical isolate available, and the passage number has been kept to a minimum in hopes of obtaining a meaningful comparison with the vaccine viruses. The issue of additional nucleotide changes arising during cell culture passage also applies to sequence analysis of vaccine virus genomes. Propagation of vaccine virus strains in cell culture to produce RNA for analysis can lead to genome changes that reflect adaptation to culture conditions and host cell type used for infection. This concern has been addressed in two ways. First, the number of cell culture passages was limited to three or less while generating viral stocks and RNA. Although this may result in some degree of cell culture adaptation, the additional minimal passage number is unlikely to affect greatly the majority of genomes in the infected cell population. Second, the sequence determination used RT-PCR products rather than cloned cDNA fragments. Sequence obtained from RT-PCR products should represent the majority sequence in a population of viral genomes and help alleviate the influence from minor viral populations that began to evolve during passage in Vero cells.Leader and trailer transcriptional control regions. The 107 3'-terminal noncoding nucleotides include the MV genomic promoter (43, 44) and are referred to here as the leader TCR (Fig. 1). Within this TCR lie several discrete sequence elements that are thought to act in cis to modulate MV transcription, replication, and gene expression. Two of these are highly conserved, well-accepted cis-acting regulatory elements of the TCR. One is the terminal 16 bases of the leader (Fig. 1). The second is the GS signal that directs N gene mRNA synthesis (2, 16, 19, 23, 27, 41). Additional cis-acting elements in the leader TCR [B-box and G(N)5 sequences] have been proposed based on genomic sequence comparisons (2, 8) and analyses of defective interfering RNAs (44), and the existence of these proposed elements has been substantiated by studies with Sendai virus (52). Nucleotide substitution in any of these elements or yet-undefined cis-acting sequences could have a significant effect on virus-cell interaction through changes in replication or gene expression efficiency.
Comparison of leader TCR sequences of Edmonston wt and the five vaccine strains revealed nucleotide differences at three positions (Fig. 2A). Nucleotide transversions were detected in all vaccines at positions 26 and 42. The wt U residue in the negative-sense genome strand was changed to an A residue at position 26. The wt U residue at position 42 was substituted with a G in AIK-C, Moraten, Schwarz, and Rubeovax, and an A residue in Zagreb. The location of these base substitutions is shown in Fig. 2A relative to previously described sequence motifs (Fig. 1). The position 26 and 42 base substitutions were located between the terminal leader TCR domain and the GS sequence (Fig. 2A; see also Fig. 7A). The third leader TCR substitution was detected only in Zagreb at position 96. This C-to-U transition occurred within the B box sequence and at the 5' boundary of the G(N)5 motif.
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Intergenic regions.
Three variable nucleotide positions (Fig.
3A) were found in the intergenic region
between the N and P genes (N/P). None of these nucleotide substitutions
were common to all vaccines. Two were in the N cistron affecting the
region that specifies the 3'-untranslated region of the N mRNA. These
substitutions included a U-to-C substitution at position 1702 shared by
all vaccine viruses except AIK-C. In the same region, Moraten and
Schwarz contained a G-to-U transversion at position 1724. Within the P
cistron, a C-to-U transition was identified at position 1806. This
nucleotide substitution was found in Moraten, Schwarz, and Rubeovax,
and it changed the base immediately upstream of the P protein
translation start codon.
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7 relative to the M gene
translation initiation codon.
Nine different nucleotide positions differed from wt in the long M/F
intergenic region (Fig. 4). None of these
mutations were found within the GE/GS signal. Four of the base changes
were detected in the 3'-untranslated region of the M gene mRNA. These
were at genomic positions 4536 (C to A in AIK-C), 4574 (C to U in
AIK-C), 4608 (A to G in Rubeovax), and 4611 (G to A in AIK-C and
Zagreb). The five remaining M/F intergenic region changes occurred
within sequences encoding the 5'-untranslated region of the F gene
mRNA. Two of these base substitutions were found only in one vaccine strain, at genomic positions 5030 in AIK-C (G to A) and 5308 in Rubeovax (A to G). The other three were conserved in all vaccine strains. These changes were two A-to-G transitions at 4978 and 5349 and
a G-to-C transversion at 5073.
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DISCUSSION |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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These analyses, though limited by the considerations described earlier, revealed distinctive base substitutions within multiple noncoding region sequences that perform potential cis-acting functions (Fig. 2 to 5). Analyses of human PIV3 and RSV A and B vaccine candidates also have identified base substitutions in cis-acting regions (7, 14, 18; D. A. Buonagurio, personal communication), indicating that this characteristic is shared by negative-strand vaccine virus strains. None of the five Edmonston vaccine viruses accumulated base changes resulting in gross alteration of a noncoding region, suggesting that these sequences do contain important components of the viral regulatory apparatus and that alteration is not well tolerated. It may also suggest that only subtle adjustments to cis-acting sequences controlling gene expression and replication were required to facilitate growth in the semipermissive cells used for vaccine virus selection.
Why MV noncoding region mutations accumulate in potential cis-acting sequences is currently speculative, but it seems reasonable to suggest that at least some of these mutations confer a selective advantage for viral replication under conditions used for vaccine derivation. Whether any of these mutations contribute to the attenuated phenotype remains to be established. Answering this question will require further studies using reverse genetics and recombinant viruses in studies like those ongoing with RSV and PIV3 vaccine candidates (7, 45, 46, 56-58).
Nucleotide substitutions in MV noncoding cis-acting sequences may be a favored response to changes in viral polypeptides induced by passage in heterologous cell types. In one simple model describing the biological selection process (33), it has been proposed that selective pressure is initially driven by a requirement for interaction between semipermissive host cell factors and viral proteins involved in the earliest stages of viral infection, particularly transcription and replication. It follows that the early stages of infection in semipermissive cells would be inefficient because viral proteins are not well equipped to deal with the semipermissive host cell environment. As the passage process progresses, mutations in viral protein coding regions are favored if they enhance the ability of viral proteins to functionally interact with proteins found in the semipermissive host cell. Although these protein modifications may enhance the level of interaction between viral and cellular proteins, they may be costly to other aspects of the virus life cycle. To help compensate for any adverse effects of amino acid substitutions, the virus evolves second-site compensatory mutations that subtly change gene expression and replication through base changes in cis-acting regulatory sequences and additional amino acid substitutions. As implied by this model, many of the noncoding region base changes identified in the Edmonston vaccines seem to have the potential to modify cis-acting regulatory components of the virus genome.
The noncoding region base substitutions can be grouped into three
categories based on their potential to modify cis-acting regulatory functions: (i) base substitutions that may affect protein translation through changes in mRNA stability or modification of
translational regulatory elements in message, (ii) base substitutions that could affect mRNA synthesis by altering promoter activity in the
leader TCR or GE/GS signal function, and (iii) base substitutions that
could affect the activity of replication promoters in the leader or
trailer TCRs. Below, the identified MV vaccine noncoding base
substitutions and their attenuation potential are discussed in the
context of these categories. Figures 2 to 5 provide the sequence data
for each gene region, while Fig. 6
summarizes these results schematically.
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Base substitutions and cis-acting regulation of translation. Mutations within the 5'- or 3'-untranslated region of mRNAs can alter protein expression by affecting mRNA stability or translation efficiency (22, 29). The combination of noncoding sequence and poly(A) tail at the 3' end of mRNAs is known to play an important role in determining mRNA stability (22). Destabilizing sequences are generally AU-rich and commonly contain the pentamer AUUUA. It is plausible that mutations in the 3'-untranslated region of MV mRNAs could affect a destabilizing sequence by interrupting an AU-rich motif or altering the secondary structure near a destabilizing motif. Base substitutions in 3' noncoding regions were found in multiple locations, including the N, M, H, and L genes. None of the mutations affecting the 3'-untranslated regions of MV mRNAs, except possibly the base substitution at position 15843 in Zagreb (Fig. 2B), obviously affects an AU-rich element (Fig. 2 to 6), but the potential of any of these base changes to alter mRNA stability can only be determined experimentally.
The 5' end of several vaccine virus mRNA sequences included base substitutions located in positions that may affect translation initiation (Fig. 6 and 7C). In both the P (Fig. 3A and 6) and M (Fig. 3B and 6) genes, base substitutions were identified near the AUG translation initiation codon in the Kozak element (Fig. 7C). The Kozak consensus sequence influences AUG codon selection and efficiency of translation initiation (24). In the P gene of Moraten, Schwarz, and Rubeovax, a G-to-A transition (mRNA sense) was found at position1 relative to the AUG codon for
the P open reading frame (Fig. 7C). In the Moraten and Schwarz M gene,
a U-to-G transversion (mRNA sense) occurred at position 7 relative to
the AUG codon (Fig. 7C).
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7 that was changed in the Moraten and Schwarz vaccines. Similarly, the P mRNA lacks a purine at position 3, which is the most
highly conserved base in the Kozak element (24). Absence of a purine at 3 will likely increase the importance of other bases
in the P mRNA Kozak element, such as the base at position 1 that was
changed in Moraten, Schwarz, and Rubeovax. If these changes in
initiation codon context alter translation efficiency, the effect may
be quite subtle since the codon context is changed only by a single
base substitution. This does not mean that these base substitutions are
not important since it is possible that attenuation is the result of
the cumulative effect of numerous relatively small adjustments in the
virus life cycle.
The poor agreement between the P gene AUG context and the Kozak element
consensus may have evolved to permit a certain percentage of ribosomes
to scan through the P AUG codon and engage the C protein mRNA
AUG codon located downstream. This also suggests that base changes
in the P Kozak element may influence the ratios of P, V, and C protein
synthesis in the infected cell. The possibility that the P AUG
codon context may influence translation of C protein has been
examined previously. Alkhatib et al. (1) placed the P gene
into a recombinant adenovirus and analyzed the levels of P and C
protein synthesis in infected cells. Deletion of the P AUG
codon had little effect on the synthesis of C protein in this system. This implies that C protein mRNA AUG codon strength is independent of the presence of the upstream P gene AUG codon and further implies that altering the P mRNA Kozak element would have little if any effect on C protein synthesis. This conclusion, however,
may be worth reexamining in an alternative system since the P gene cDNA
was fused to the adenovirus major late transcription unit tripartite
leader sequence. These adenovirus sequences function as a
cis-acting translational control element in
adenovirus-infected cells (42) and may negate some of the
intrinsic cis-acting elements found in the measles virus P
mRNA. Clarification of possible interplay between the P and C
mRNA AUG codons may best emerge from study of appropriately
designed recombinant MVs.
Further evidence linking translation and attenuation may be found by
examining the F gene. MV has maintained the 1-kb intergenic region
between the M and F genes that in part specifies a long untranslated 5'
end for the F mRNA (Fig. 1 and 4). This untranslated sequence is
dispensable for recombinant virus growth in a Vero cell culture
(36). However, it has been found to modulate translation and play a role in the selection of a predominant initiation
codon from several closely positioned AUG sequences that are in
frame with the F coding region (Fig. 1 and 4 and reference
5). In comparing Edmonston strains, five base changes were
detected in the vaccine virus F gene mRNA untranslated 5' end (Fig.
4). Given that the F mRNA 5'-untranslated sequence functions as a
cis-acting element and that three base substitutions in
this sequence were common to all vaccine strains, it seems likely that
modification of this sequence may have been favored for MV growth in
semipermissive cell types. Whether the F mRNA untranslated region
plays a role in attenuation remains uncertain, but studies showing that
recombinant virus lacking most of this sequence replicates less
efficiently in human thymus-liver tissue transplanted in SCID mice
(55) suggests that the untranslated region may play a role
in pathogenicity.
Base substitutions and the control of mRNA synthesis and processing. The second category encompasses mutations with potential to influence viral mRNA synthesis, specifically mutations in GE/GS signals and the leader TCR. One such base substitution occurred within the F/H intergenic GE/GS signal (Fig. 7A). This mutation was found in Moraten and Schwarz, where an A-to-G transition occurred in the GE signal (nucleotide position 7243; Fig. 7A). The effect of this base substitution is not known as yet, but it may perturb normal transcription termination and reinitiation at the F-H gene boundary, leading to altered expression of the downstream H gene. This phenomenon has been observed in some strains of RSV, PIV, and simian virus 5, where less-efficient GE signal variants cause reduced transcription termination with overproduction of readthrough bicistronic transcripts at the expense of normal monocistronic mRNA synthesis of the downstream gene (3, 37, 47, 54) (D. A. Buonagurio, personal communication). Reduction in correctly initiated mRNA synthesis from the downstream gene, coupled with the likelihood that the fused bicistronic transcripts are inadequate templates for translation of the gene distal to the 5' mRNA cap (24, 60), will restrict protein synthesis encoded by the downstream gene. This effect could result in diminished H protein expression if Moraten and Schwarz produce elevated levels of a F/H bicistronic message.
It also may be relevant to mention the mutation at position 42 in this context even though it is possible that the GE signal located in the leader TCR may not function analogously to GE signals found in intergenic regions. The position 42 mutation was present at the 3' boundary of the GE/GS signal consensus drawn in Fig. 7A, raising the possibility that it may modify GE/GS signal function. This could be significant if transcription initiating at the genomic 3' end must terminate and reinitiate to effectively transcribe an N mRNA and the position 42 mutation alters termination or reinitiation function. Whether a termination and reinitiation mechanism applies to mRNA synthesis initiated from the MV leader TCR is uncertain given the failure to detect abundant small leader RNA products in infected cells that would be indicative of termination prior to the N gene GS signal (4, 8). However, it is worth noting that attempts to detect the small leader RNA in infected cells have relied on infection with laboratory-adapted Edmonston strains that have vaccine leader mutations. If the base 42 substitution negatively affects termination efficiency, greater accumulation of leader-N mRNA fusions will result. These readthrough transcripts introduce an AUG sequence upstream of the authentic N gene initiation codon, potentially reducing N protein expression. In addition, leader sequences attached to N mRNA should serve as substrates for interaction with N protein. Encapsidation of leader-N gene fusion mRNAs should render them unavailable for translation. Given these considerations, the position 42 base substitution could reduce the level of N protein synthesis in all vaccine viruses by altering GE function. mRNA levels also may be influenced by promoter strength of the leader TCR. As noted above, three base changes were observed in this region of the vaccine viruses. One was a C-to-U transition that was unique to Zagreb at nucleotide position 96 (Fig. 2B). The other two mutations were at positions 26 and 42. The position 26 and 42 pyrimidine-to-purine transversions were conserved in all vaccine strains. Alignment of leader TCR mutations to previously described sequence motifs (Fig. 1, 2, and 7B) showed that the position 26 mutation was excluded from any currently described motifs while, as described earlier, the base change at 42 lay within the boundaries of the GE/GS consensus sequence (Fig. 7A). The unique Zagreb mutation at position 96 (Fig. 7B) was present within the boundaries of the B box (2, 8) and the overlapping G(N)5 motif (52). The possibility that these leader TCR mutations affect mRNA synthesis is speculative but intriguing to consider further. It is noteworthy that the leader TCR changes at 26 and 42 were located within a region that is not highly homologous to the trailer TCR (Fig. 8). Considering the functional similarity between the leader and trailer TCRs, it seems reasonable to expect significant sequence homology in regions that perform largely identical functions. Alignment of both TCRs shows, as described before (2, 8), that they share two regions of strong homology. One includes the terminal 16 bases, and the second encompasses the B and B' boxes. The fact that only the Zagreb mutation (position 96; Fig. 2 and 6 to 8) fell within one of these homologous sequences (the B-box region) may indicate that these sequence elements are relatively intolerant of nucleotide substitutions. Alternatively, it may imply that virus passage in semipermissive cells favored changes in sequences that were unique to leader TCR. This may further imply that these leader TCR changes were favored because they modulated a leader TCR-specific function such as the initiation of mRNA synthesis.
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Leader and trailer TCR base substitutions and the control of genome replication. Base substitutions in the leader or trailer TCR could influence replication by altering the efficiency of genome-length RNA synthesis. As described above, three base substitutions were found in the leader TCR. While it was suggested that these mutations might act at the level of mRNA transcription, they may additionally or alternatively have an impact on the initiation of antigenome synthesis or modulate the pathway that controls the preferential selection of antigenome or mRNA synthesis. In that context, the unique Zagreb mutation at position 96 present in the B box is interesting because its location in a motif that is conserved in both the leader and trailer TCRs suggests that this homologous sequence domain regulates the replication pathway.
Only two base changes were found in the trailer TCR, and these were specific to individual vaccine strains. Neither the AIK-C mutation (position 15789) nor the Zagreb mutation (position 15843) lie within the 16-base conserved terminal promoter sequences or the overlapping B box-G(N)5 domains. Although localized to the trailer TCR, these base substitutions also fall into the category of mutations that modify untranslated regions of mRNAs. Both mutations reside in the 3' noncoding region of the L mRNA. Were they to affect the phenotype of these vaccine strains, it would likely be through the changes in the L mRNA stability. Additionally, the Zagreb trailer TCR mutation is positioned at the GE/GS consensus region (Fig. 8). Although it does not function as a GE/GS signal in the positive genome strand, this relative positioning could indicate that this region makes contact with the polymerase complex bound at the trailer. It remains to be determined if this Zagreb mutation affects the replicative function of the trailer. Beyond their putative capacity to influence transcription and replication, the mutations in leader and trailer TCRs can theoretically affect encapsidation efficiency. During genome synthesis, the first sequences that become accessible for encapsidation are the leader and trailer, suggesting that nucleation sites mediate encapsidation within these regions. Thus, base substitutions in the leader or trailer could affect the interaction of N protein with nascent genomes. This seems a less likely scenario given studies with vesicular stomatitis virus delimiting the encapsidation signal to the terminal 14 bases (41), a region that was unaffected by base substitutions amongst the Edmonston MV strains.Contribution to attenuation. Not all of the vaccine virus noncoding region base changes are expected to have equivalent impact on replication or gene expression and the levels of attenuation. The base changes found in the P and M mRNAs, within the Kozak consensus sequence, might affect protein expression either positively or negatively. The alteration in the M mRNA AUG context could influence virion maturation and possibly gene expression (16, 49, 59) if the levels of M protein synthesis are altered. The case of the P initiation codon is particularly interesting because changes in the AUG codon strength could affect the translation of P (and V) as well as the translation of C protein from the downstream AUG. Regulation of P, V, and C mRNA translation as a means of achieving attenuation is particularly attractive because each of the P gene-encoded proteins, and their balance, likely play a central role in controlling genome replication and mRNA transcription (16, 19, 41). Given these multiple and vital functions, even small changes in the relative concentrations of P, V, and C proteins in the infected cells could significantly influence viral replication in the infected host (12, 31, 34, 53, 55).
Base changes in the untranslated leader of the F gene also may contribute to the attenuated phenotype. Expression of F protein is essential for cell fusion and effective cell-to-cell spread of MV (16, 59), and it has been demonstrated that the F gene 5'-untranslated region plays a role in determining translation efficiency and AUG codon selection (5). The fact that three mutations were common to all vaccine F gene mRNAs implies that passage in semipermissive cells selected for alteration in this cis-acting element. The most obvious advantage accrued by base substitutions in this region is their ability to alter the levels of F protein expression levels, perhaps through changes in the higher-order structure of the mRNA 5' end. Perturbation of this function may also be responsible for the reduced viral titers observed in the human thymus-liver tissue-SCID mouse model system (55) when the thymic implants are infected with a recombinant Edmonston B strains that lacks much of the sequence for the F mRNA 5'-untranslated region. Base changes in the leader TCR also are likely attenuation candidates. It was proposed above that the position 26 and 42 pyrimidine-to-purine transversions may influence gene expression by changing the interaction between the promoter and the viral polymerase. In this scenario, base substitutions disturbing normal levels of mRNA transcription certainly might contribute to an attenuated phenotype. It is also noteworthy that amino acid coding changes were identified in vaccine virus genes for the polymerase complex proteins (P and L) and accessory proteins (C and V) (33). Possibly, the vaccine virus base changes in the leader TCR and the amino acid changes in protein components of the gene expression apparatus have evolved together to optimize expression in semipermissive cells such as chicken fibroblasts. A by-product of these changes is that upon infection of the human host this adapted form of the transcriptional apparatus is less efficient and leads to an attenuated phenotype. Finally, these comparative analyses included comparison of highly related vaccine viruses that differ in attenuation level. Optimally attenuated Moraten and Schwarz were found to contain identical noncoding region sequences, and these sequences differed from the underattenuated Rubeovax strain by only five nucleotides (Fig. 6, nucleotides highlighted with a dashed box). Two of these differences were substitutions unique to Rubeovax, and these were both T-to-C transitions (mRNA sense) in the M/F intergenic region. One of these base substitutions was present in the M gene mRNA 3'-untranslated sequence, and the other was present in the F gene mRNA 5'-untranslated region. These mutations could affect expression by changing mRNA stability or efficiency of translation. The third base that distinguished Rubeovax from Moraten and Schwarz was found in the 3'-untranslated region of the N mRNA. In this case, Rubeovax contained a wt nucleotide compared to the base change contained in the other two vaccines. Again, if this base contributes to a difference in attenuation levels it would probably be the result of altered mRNA stability. The final two bases that distinguish Rubeovax from Moraten and Schwarz have somewhat more compelling links to attenuation. These two substitutions in Moraten and Schwarz remained wt in Rubeovax. They altered a base in the M mRNA Kozak consensus sequence at position 3431 and a base in the F gene GE signal at position 7243. As mentioned earlier, the base change in the M gene could influence translation of M protein resulting in altered virion maturation (59) and possibly transcription regulation (49). The F GE signal mutation may similarly downregulate expression of the downstream H gene if it compromises transcription termination or subsequent reinitiation. One or more of these five features may be responsible for subtle differences between Moraten and Schwarz and the underattenuated Rubeovax strain that helps account for the difference in attenuation levels.| |
ACKNOWLEDGMENTS |
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C.L.P. and R.A.L. contributed equally to this work.
We thank William Bellini and Paul Rota (Centers for Disease Control) for providing MV vaccine strains. We also thank Judy Beeler (Center for Biologics Evaluation and Research, FDA) for providing a low-passage wt virus. We also appreciate Martin Billeter's careful review of the manuscript.
The initial stages of these studies were supported by NIH grant AI35286 to S.A.U.
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FOOTNOTES |
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* Corresponding author. Mailing address: Department of Viral Vaccine Research, Wyeth-Lederle Vaccines, 401 North Middletown Rd., Pearl River, NY 10965-1299. Phone: (845) 732-5450. Fax: (845) 732-5727. E-mail: udems{at}war.wyeth.com.
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REFERENCES |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Journal of Virology, January 2001, p. 921-933, Vol. 75, No. 2
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.2.921-933.2001
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