TEXT

Top Abstract Text References

The P143 protein of Autographa californica nuclear polyhedrosis virus is an essential protein that was predicted to be a DNA helicase based on the presence of an ATP binding motif that is conserved among DNA helicases (3, 9). Subsequent site-directed mutagenesis studies on seven putative helicase motifs revealed that mutations in motifs I, Ia, II, and III blocked DNA replication, while mutations in the other motifs had no effect (7). This result supports the classification of P143 as a member of superfamily III, which is a relatively uncharacterized group of enzymes that lack recognizable sequences corresponding to motifs IV to VI (2).

P143 is required for transient replication of a plasmid containing a baculovirus origin of DNA replication in addition to IE1, DNApol, LEF-1, LEF-2, and LEF-3 (5, 10). P143 copurifies with LEF-3, the baculovirus single-stranded DNA (ssDNA) binding protein (SSB) (1, 4). This interaction between P143 and LEF-3 is not surprising because many helicases specifically bind their cognate SSBs, and this interaction has been shown to stimulate DNA unwinding (11). In the case of P143, however, the LEF-3 interaction also has an unexpected function because LEF-3 is required for nuclear localization of P143 (15).

Recently, we showed that P143 has both ATPase and DNA unwinding activities (12). As expected for a helicase, P143 binds to double-stranded DNA (dsDNA) and ssDNA in a sequence-nonspecific manner (6, 12). In electrophoretic mobility shift assays (EMSAs), ladders of shifted bands are observed in an enzyme-dependent manner, suggesting that multiple monomers or oligomers bind to both probes in a noncooperative manner.

ATP inhibits the DNA binding activity of P143. Our aim in this study was to further analyze the DNA binding activity of P143 in order to gain insight into the mechanism of DNA binding and DNA unwinding activities. As a first step, we compared the DNA binding activity of P143 in a standard EMSA buffer and in a buffer optimized for ATPase activity. We found that DNA binding was considerably lower in the ATPase buffer (data not shown). Analysis of each of the different components revealed that the formation of stable protein-DNA complexes was decreased approximately fivefold by the addition of ATP and MgCl₂ (Fig. 1, lanes 6, 7, 13, and 14). This experiment was performed with two different concentrations of enzyme in order to cover a wider range of activity. Furthermore, addition of ATP alone had an even more dramatic effect on DNA binding (Fig. 1, lanes 4, 5, 11, and 12). This was true for both dsDNA (Fig. 1, lanes 1 to 7) and ssDNA (Fig. 1, lanes 8 to 14).

View larger version (82K): [in this window] [in a new window]   FIG. 1.   Effects of ATP and MgCl2 on the binding of P143 to 252-bp hr5 ss- and dsDNA. The conditions for purification of P143, radiolabeling of hr5 probe, and EMSAs have been described previously (12). In this experiment, the molar ratios of enzyme to probe were 10:1 and 50:1. The presence or absence (+ or ) of 2 mM ATP and 6 mM MgCl2 is indicated at the top. Lanes 1 to 7 contain dsDNA probe; lanes 8 to 14 contain ssDNA probe. ssDNA probe was generated from the double-stranded probe by dilution in water followed by boiling and rapid cooling on ice. Free and protein-bound DNAs were separated on a 3.5% acrylamide-Tris-borate-EDTA gel, dried, and exposed to PhosphorImager plates. ds, double-stranded; ss, single-stranded.

Inhibition is specific for ATP. A trivial explanation for the inhibition of DNA binding is that ATP altered the pH or ionic strength of the buffer, even though the nucleotide solution was neutralized to pH 7.0 before use. We reasoned that if inhibition were due simply to the presence of a negatively charged nucleotide that competed with DNA, then other nucleoside triphosphates (NTPs) should have the same effect. To test this, we added eight different NTPs individually to identical reaction mixtures, each at the same concentration. For these experiments, a shorter DNA probe, corresponding to an 88-bp fragment of hr5, was used because it produced only one or two shifted bands. This allowed for better quantitation than did the larger probe, which produced multiple bands containing unknown amounts of protein in each shifted band. The EMSA results showed that DNA binding was inhibited only in the reactions containing ATP (Fig. 2). The other seven nucleotides did not significantly alter the binding of P143 to DNA. Previously, we showed that ATP was the only NTP that could support the DNA unwinding activity of P143 (12). Therefore, this suggests that inhibition is due to binding of the enzyme to ATP and not to nonspecific ionic effects.

View larger version (107K): [in this window] [in a new window]   FIG. 2.   Effect of NTPs on P143 DNA binding in the absence of divalent cation. Lane 1 contains probe alone; lanes 2 to 10 contain purified P143 in fivefold-molar excess of enzyme over an 88-nucleotide radiolabeled DNA probe. Lanes 3 to 10 contain a 2 mM concentration of the indicated NTP. Free and protein-bound DNAs were fractionated on a 5% acrylamide-Tris-borate-EDTA gel, dried, and exposed to a PhosphorImager plate. An 88-nucleotide probe, also containing hr5 sequences, was cut from the same plasmid, first with EcoRI and then, after kinase labeling, with MluI.

Only divalent cations that support ATP hydrolysis restore DNA binding. We then wanted to determine whether the ability of MgCl₂ to reverse the ATP inhibition of DNA binding was related to the catalytic activity of the enzyme. If MgCl₂ were only acting as a counterion, then other divalent cation salts should have the same effect. Alternatively, if MgCl₂ restored DNA binding because it supported ATPase activity, then only cations that also function as cofactors should relieve inhibition.

View larger version (41K): [in this window] [in a new window]   FIG. 3.   Effects of divalent cations. (A) ATPase. Each reaction mixture contained a 6 mM concentration of the indicated divalent cation salt. ATPase reaction mixtures contained 2 mM ATP, 0.1 µCi of [-32P]ATP, a 6 mM concentration of the indicated divalent cation chloride salt, 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 15 mM NaCl, 1 mM dithiothreitol, 140 fmol of P143, and 0.25 µg of activated calf thymus DNA in a final volume of 20 µl. Samples were incubated at 30°C for 30 min and were then terminated by the addition of 25 mM EDTA. Polyethyleneimine thin-layer plates were spotted with 1 µl of each reaction, and the hydrolyzed, radiolabeled phosphate was separated from the input triphosphate by development in 1 M formic acid and 0.5 M LiCl. Thin-layer chromatography plates were dried and exposed to PhosphorImager screens. Results were quantitated using a Storm PhosphorImager. Background levels of hydrolysis in the absence of enzyme were subtracted. An assay was performed in triplicate, and standard error is indicated. (B) DNA binding. Lane 1 contains probe without enzyme. Lanes 2 to 7 contain P143 in a fivefold-molar excess over the 88-nucleotide radiolabeled probe. Lanes 3 to 7 contain 2 mM ATP (+), while lane 2 does not (). Lanes 4 to 7 contain a 6 mM concentration of the indicated divalent cation salt. Reaction mixtures were fractionated on a 5% acrylamide-Tris-borate-EDTA gel, dried, and exposed to a PhosphorImager plate.

ATP and MgCl₂ increase the rate of dissociation. We hypothesized that the DNA binding affinity of P143 was higher in the absence of its substrate and cofactor because it was not engaged in ATP hydrolysis. We tested this idea by assembling two binding reactions, one in the presence of ATP and MgCl₂ and one in the absence. The enzyme was incubated on ice for 20 min to allow binding to the radiolabeled double-stranded 252-bp probe. Then, a 100-fold-molar excess of the same unlabeled DNA fragment was added (aliquots were removed at the indicated times) and was loaded onto a gel with the current running continuously. This allowed us to compare the rate of dissociation of P143 from the DNA under both sets of conditions (Fig. 4).

View larger version (71K): [in this window] [in a new window]   FIG. 4.   The effect of ATP and MgCl2 on the dissociation rate of P143 DNA binding. Lanes 2 to 21 contain a fivefold-molar excess of P143 over a radiolabeled 252-bp dsDNA probe in the absence or presence (+ or ) of 2 mM ATP and 6 mM MgCl2 as indicated at the top. After 20 min of incubation, a 100-fold molar excess of unlabeled probe was added to each reaction mixture, and 20 µl was removed at the times indicated at the top of the gel and was loaded on a 3.5% nondenaturing polyacrylamide gel with the current running continuously (lanes 6 to 21). Lane 1 contains the probe in the absence of enzyme; lanes 2 and 3 contain probe and competitor added together; lanes 4 and 5 had no competitor added. Free probe shifts up with time because these samples were loaded later and, therefore, electrophoresed for a shorter period of time.

A model for DNA binding and ATPase activities of P143. The DNA binding data presented here suggests a model in which P143 undergoes conformational changes upon binding and hydrolysis of ATP. These conformational changes alter its affinity for DNA and presumably involve translocation and/or DNA unwinding. This suggests that helicase does not unwind DNA by the rolling mechanism because a fundamental requirement of that model is that a subunit of the helicase is in contact with the DNA at all times (13, 14). The inchworm model (16) is more consistent with our findings because it proposes that helicases undergo conformational changes that advance the enzyme forward into the double-stranded region of DNA before ATP hydrolysis. In order to slide forward on the DNA, the enzyme must release the nucleotides in the DNA binding pocket. This is consistent with the finding that P143 binds DNA more efficiently in the absence of ATP and MgCl₂; the enzyme is static in the absence of cofactors and therefore binds DNA with high affinity. In the presence of ATP and MgCl₂, P143 goes through cycles of hydrolysis and translocation. EMSA captures only those molecules that are bound with high affinity, while those in the process of translocation temporarily dissociate from DNA and so do not produce shifted bands.