INTRODUCTION

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Human immunodeficiency virus type 1 (HIV-1) primarily infects CD4⁺ T lymphocytes and cells of monocyte-macrophage lineage. The cellular tropism of HIV-1 is determined largely at the level of virus entry, which depends on a series of interactions between viral envelope glycoprotein and cellular receptors. The gp120 surface glycoprotein subunit is thought to interact first with CD4 (the primary receptor) and then with one (or more) of the recently identified coreceptors, which include chemokine receptors CXCR4 and CCR5 (for reviews, see references 15, 16, 26, and 32). These interactions are thought to trigger conformational changes in the complex multimeric viral envelope glycoprotein structure, allowing the hydrophobic domain of transmembrane glycoprotein gp41 subunit to interact with the cellular membrane and induce virus-cell fusion.

While the exact mechanism of membrane fusion is still unclear, the interactions between gp120 and cellular receptors are slowly beginning to be understood. The detailed molecular nature of the interactions between gp120 and CD4 has been elucidated from analysis of a crystal structure of gp120 core complexed with CD4 (two N-terminal domains) and the 17b neutralizing monoclonal antibody (Fab fragment), which interacts with the putative coreceptor binding domain of gp120 (22). The interactions between gp120 and its coreceptors have been investigated using a multitude of indirect experimental approaches, including site-directed mutational analyses, functional and infectivity studies with chimeric proteins and viruses, and biochemical competition experiments with site-specific antibodies and chemokines (5-7, 10, 11, 14, 21, 23, 35, 40-42, 45-47, 49, 52). The accumulated data from these studies suggest that the variable loops V1/V2 and V3, which likely form a pocket surrounding the four-stranded antiparallel -sheet (bridging sheet), play important roles in coreceptor interactions. Depending on the conformation of these loops, gp120 binds to CCR5, CXCR4, or both, thus determining the cellular tropism of the virus isolate (macrophage [M]-tropic, T-cell line [T]-tropic, or dualtropic, respectively).

HIV-1 gp120 is one of the most extensively glycosylated proteins (33). It contains 23 or 24 N-linked glycosylation sites, and the glycans attached to these sites account for approximately one-half of the protein's total mass (based on polyacrylamide gel mobility). Numerous studies using glycosylation and glycosidase inhibitors have revealed the importance of the carbohydrate moieties in determining the conformation of the HIV-1 envelope glycoprotein, a property that undoubtedly affects its processing, intracellular transport, and ability to interact with CD4 (13, 17, 19, 29, 38, 50). The gross modifications resulting from the use of these inhibitors, however, are not as informative as site-directed mutagenesis, which permits evaluation of the effects of individual glycans on protein structure and function. For example, site-directed mutagenesis of all 24 individual N-linked glycosylation sites of HIV-1_HXB2 indicated that most of the glycosylation sites were individually dispensable (25). Of the 24 sites, only 5 (amino acids 88, 141, 197, 262, and 276), all of which are located in the amino-terminal half of gp120, affected virus infectivity.

Most site-directed mutagenesis studies have been conducted with gp120s of T-tropic laboratory-adapted HIV-1 strains (e.g., HXB2 or NL4-3). Several studies have demonstrated that these envelope glycoproteins have biochemical and immunological properties which differ from those of primary HIV-1 isolates (e.g., greater gp120 shedding and increased susceptibility to neutralizing antibodies or CD4 [1, 12, 30, 31, 48, 55; for a review, see reference 39]). We have previously characterized cellular tropism and coreceptor usage of a primary isolate, HIV-1_DH12 (7, 23, 44), a dualtropic virus that can utilize CXCR4 and CCR5 almost equally and can infect both T-cell lines and primary monocyte-derived macrophages (MDM). In the present study, we examined the role of carbohydrate moieties in cellular tropism and coreceptor usage of HIV-1_DH12 by mutagenizing specific N-linked glycosylation sites throughout gp120. The mutant envelope proteins were examined for their capacity to induce cell-to-cell fusion. Some of the mutants were further analyzed in the context of virus infectivity in both T-cell line and primary cells. Our results indicate that the N-linked glycosylation sites near the V1/V2 and V3 variable loops are critical for the induction of membrane fusion and virus entry.

	MATERIALS AND METHODS

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Envelope glycoprotein mutagenesis. Mutations affecting the N-linked glycosylation sites were introduced into plasmid pTM-DHgp120H (24), which encodes HIV-1_DH12 gp120. Asparagine (N) codons AAT or AAC were changed to glutamine (Q) codon CAG by using the mutagenic oligonucleotides listed in Table 1. Codon changes were made using the Quik Change site-directed mutagenesis kit according to the manufacturer's protocol (Stratagene). N-linked glycosylation site mutations (µ) were subsequently transferred to pNVV-DHenv (7), which encodes the entire gp160 of HIV-1_DH12. The mutations in pTM-DHgp120H µ1-µ3 were transferred to pNVV-DHenv by replacing the 488-bp KpnI-StuI fragment (nucleotides [nt] 120 to 608) to generate pNVV-DHenv µ1 to µ3. For pNVV-DHenv µ4 and pNVV-DHenv µ5 to µ10, the 787-bp EcoNI fragments (nt 600 to 1387) and the 559-bp BglII fragments (nt 817 to 1376), respectively, were transferred from the corresponding pTM-DHgp120H clones. The plasmids pNVV-DHenv µ2-1, µ2-2, µ3-1, µ3-2, µ8-1, and µ8-2 were generated directly from pNVV-DHenv with the same site-directed mutagenesis protocol using the oligonucleotides shown in Table 1.

Protein expression and Western blotting. The recombinant vaccinia viruses vvDHenv µ1 to µ10 were generated from corresponding pNVV-DHenv µ1 to µ10, following protocols previously described (9). Confluent monolayers of HeLa cells in 6-well plates were infected with recombinant vaccinia viruses at a multiplicity of infection of 10. Cell lysates were prepared by adding lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 1.5 mM MgCl₂, 1% NP-40) 48 h postinfection. Culture supernatants and cell lysates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by electrotransfer to nitrocellulose (Hybond; Amersham Life Science) for a Western immunoblot assay. Envelope glycoproteins were detected by blotting with rabbit anti-gp160 antiserum (53) followed by goat anti-rabbit immunoglobulin G-peroxidase conjugate and visualizing with an ECL Western blot detection kit according to the manufacturer's protocol (Amersham Life Science).

Fusion assay. A highly sensitive secreted alkaline phosphatase (SEAP) reporter gene-based assay was used to quantitate cell-cell fusion events as previously described (23). To prepare target cells, recombinant vaccinia viruses encoding CCR5 (vvCCR5) or CXCR4 (vBD4 [3]), T7 RNA polymerase (vTF7-3 [18]), and human CD4 (vCB-3 [4]) were used to coinfect Mus dunni cells. To generate vvCCR5, the CCR5 gene was PCR amplified from a DNA preparation from human peripheral blood mononuclear cells PBMC using the following primers: (+), 5'-CTGAGGATCCCATATGGATTATCAAGTGTCA-AGT-3', and (), 5'-GATCTTAAGCTTCTAGATCAGTGATGGTGATGGTGATGCGATCC-TCTCAAGCCCACAGATATTTC-3'. The conditions for PCR were as follows: 94°C for 7 min; 3 cycles of 94°C for 1 min, 40°C for 1 min, and 72°C for 3 min; then 30 cycles of 94°C for 1 min, 55°C for 1 min, and 72°C for 3 min; and finally 72°C for 7 min. The PCR product was digested with BamHI and HindIII (incorporated in the primers) and cloned into the same restriction sites in pGEM-3Zf() (Promega, Madison, Wis.) to generate pGEM-CCR5. Subsequently, the 1,100-bp SmaI-XbaI fragment from pGEM-CCR5, which contains the CCR5 gene, was cloned into SmaI-AvrII sites of pNVV-3 (provided by M. Oldstone). The resulting plasmid, pNVV-CCR5, was used to generate recombinant vaccinia virus vvCCR5 following protocols previously described (9). To prepare the effector cells, M. dunni cells were coinfected with recombinant vaccinia viruses encoding SEAP under the control of T7 promoter (vTM-SEAP [23]) and either wild-type (vvDHenv [7]) or mutant (vvDHenv µ1 to µ10) envelope glycoproteins. Although some envelope mutants exhibited reduced levels of membrane-associated gp120, we used the same multiplicity of infection of the recombinant vaccinia viruses to express the same amount of the total envelope glycoprotein. Infected cells were incubated at 37°C for 5 h and then trypsinized. After the cells were washed twice with the culture medium (Dulbecco's minimal essential medium containing 10% fetal bovine serum), duplicate samples containing 5 × 10⁴ (each) target and effector cells were mixed in a 96-well plate. Cells were cultured in the medium containing 80 µg of cytosine arabinoside per ml for 8 to 10 h at 37°C. SEAP activity in the culture supernatant was measured as previously described (23).

Virus stocks and infections. The HIV-1 viruses used in this study were generated from an infectious molecular clone, pAD8-DHenv (8), which contains the env gene of HIV-1_DH12 in the background of HIV-1_AD8. Wild-type and mutant viruses were generated by transfecting respective plasmids into HeLa cells using the calcium phosphate-based Profection transfection system (Promega). Virus stocks were concentrated by ultracentrifugation (Beckman SW 55Ti rotor; 35,000 rpm for 30 min). The relative amounts of viruses were determined by measuring reverse transcriptase (RT) activity in the stocks as previously described (54). Viral infections were performed in 96-well plates using either phytohemagglutinin-blasted human PBMC, MT-4 cells, or MDM essentially as previously described (7).

Cloning the revertant env gene. Human PBMC were infected with AD8-DHenv µ8-R virus, which was initially isolated from PBMC culture supernatants at peak RT activity (day 22). Infected cells were harvested and Hirt DNA was prepared as described previously (20). A 3-kb fragment spanning the env gene of HIV-1_DH12 was amplified by PCR from the Hirt DNA using the following primers: (+), 5'-CAGTAGATCCTAGACTAGAGCCCTGG-3' (387 nt upstream from the env initiation codon), and (), 5'-GCTGCTCCCACCCCATCTG-CTGCTG-3' (98 nt downstream from the env stop codon). The conditions for PCR were as follows: 94°C for 2 min followed by 10 cycles 94°C for 20 s, 63°C for 30 s, and 68°C for 4 min; 20 cycles of 94°C for 20 s, 63°C for 30 s, and 68°C for 4 min, plus 20 additional s for each incremental cycle; and finally 68°C for 7 min. PCR was performed using the Expand long template PCR system (Boehringer Mannheim), and the PCR product was cloned using a Topo TA cloning kit (Invitrogen). The env gene of the resulting plasmid (pCR-µ8-R) was subsequently sequenced.

	RESULTS

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Mutagenesis and expression of envelope glycoproteins. To examine whether carbohydrate moieties of HIV-1 gp120 played any role in its function or structural integrity, mutations (asparagine to glutamine) were introduced into the conserved as well as variable regions of HIV-1_DH12 gp120, as depicted in Fig. 1. Either one (µ1, µ4, µ5, and µ6) or two (µ2, µ3, µ7, µ8, µ9, and µ10) potential N-linked glycosylation sites were changed throughout gp120. To determine whether these mutations had any effect on the expression and/or processing of gp160, recombinant vaccinia viruses encoding either wild-type or mutant envelope glycoproteins were used to infect HeLa cells. Cell lysates and culture supernatants were subjected to SDS-PAGE and Western immunoblotting with rabbit anti-gp160 antiserum as shown in Fig. 2a and b, respectively. Although the level of gp120 was somewhat lower for a few of the mutants (e.g., µ3 and µ4) compared to the wild-type protein, all of the mutant gp160s were expressed and processed to gp120. An increase in electrophoretic mobility was observed for each of the deglycosylated mutant glycoproteins compared to the wild type. This was more apparent for the mutants containing two mutations (µ2, µ3, µ7, µ8, µ9, and µ10). These results suggest that each of the asparagine residues we had mutated was indeed being utilized for N-linked glycosylation.

View larger version (31K): [in this window] [in a new window]   FIG. 1.   A schematic diagram of N-linked glycosylation site mutant constructs. The positions of 23 potential N-linked glycosylation sites and the variable regions of HIV-1DH12 gp120 are identified at the top. Ten initial mutant constructs (µ1 through µ10) are shown with the locations of the mutated glycosylation site(s). Mutants µ1 and µ4 through 6 have only a single site removed while the others have two sites removed.

View larger version (64K): [in this window] [in a new window]   FIG. 2.   Western immunoblot of envelope glycoproteins expressed by recombinant vaccinia viruses. Cell lysates (a) and culture medium (b) of HeLa cells infected with either wild-type or mutant envelope-expressing vaccinia viruses were subjected to SDS-PAGE followed by Western immunoblotting. The positions of molecular weight markers, gp160, and gp120 are indicated.

Coreceptor usage of N-linked glycosylation site mutants. HIV-1_DH12 is a dualtropic virus, utilizing both CCR5 and CXCR4 as coreceptors for virus entry. To examine whether deglycosylation had affected its ability to induce membrane fusion and/or coreceptor usage, we performed a highly sensitive cell-to-cell fusion assay using SEAP as an indicator (23). As shown in Fig. 3, the mutations did not appreciably disrupt coreceptor usage, except for µ2, µ3, and µ8. The fusion activity decreased to 30 to 40% of that of the wild-type protein for µ2, whereas the activity was completely abolished for µ3 with either CCR5 or CXCR4. This loss of fusion activity by µ3 was not necessarily due to reduced gp160-processing efficiency of this mutant since µ4, which exhibited a similar level of processing, induced only slightly less cell-to-cell fusion activity compared to the wild type. A different pattern of coreceptor fusion dysfunction was observed for µ8; while retaining approximately 50% of the fusion activity with CXCR4 compared to the wild type, it completely lost the activity with the CCR5 receptor. These results indicate that the carbohydrate moieties near the V1/V2 (µ2 and µ3) and V3 (µ8) loops of HIV-1 gp120 influence its capacity to induce membrane fusion, possibly by altering the structure of envelope domain(s) interacting with either CD4 and/or the coreceptors. A soluble CD4 (sCD4) binding assay indicated that the interaction of µ3 gp120 with CD4 was reduced approximately 50% compared to wild-type gp120. In contrast, µ2 and µ8 gp120s exhibited binding properties to sCD4 similar to those of the wild-type protein (data not shown).

View larger version (65K): [in this window] [in a new window]   FIG. 3.   Cell-to-cell fusion activity. Fusion activity of mutant envelope proteins with CCR5 or CXCR4 is shown as percentage of relative light units (RLU) in comparison to the wild-type protein.

Replication profile of viruses containing µ2 and µ8. To characterize the effects of glycosylation site mutations µ2 and µ8 in the context of an infectious virus, the mutations were transferred to a full-length molecular clone, pAD8-DHenv (8). This chimeric virus, which contains the HIV-1_DH12 env gene in the background of HIV-1_AD8, was used because its capacity to produce progeny virus was superior to that of the parental HIV-1_DH12 (8). The replication profiles of µ2 and µ8 viruses in human PBMC, MT-4 cells, and human primary MDM were compared to those of the wild-type virus and of another mutant virus (µ7), which did not exhibit defective cell-to-cell fusion (Fig. 4).

View larger version (29K): [in this window] [in a new window]   FIG. 4.   Replication of viruses carrying mutant envelopes. The replication of three mutant viruses (µ2, µ7, and µ8) was compared to that of the wild type in PBMC (a), MT-4 T cells (b), and MDM (c). Virion-associated RT activity in the culture medium of virus-infected cells was determined as described in Materials and Methods.

Fine mutagenesis of the V1/V2 and V3 regions of gp120. Membrane fusion and virus infectivity assays indicated that the sites mutated in the mutants µ2, µ3, and µ8 very likely play an important role(s) in envelope glycoprotein function. All three mutants carry mutations at two adjacent glycosylation sites. To ascertain the relative importance of each glycosylation site within the pair, six additional single-site mutants were constructed by individually mutating N135 (µ2-1), N141 (µ2-2), N156 (µ3-1), N160 (µ3-2), N295 (µ8-1), and N301 (µ8-2) (Fig. 5a). For the first four mutants (µ2-1, µ2-2, µ3-1, and µ3-2), recombinant vaccinia viruses were generated and the fusogenic properties of the mutant envelope glycoproteins were examined. As shown in Fig. 5b, µ2-1 exhibited fusion activity indistinguishable from that of µ2 with both CCR5 and CXCR4. In contrast, the defect was more severe for µ2-2, which retained only 10% of the wild-type fusion activity. Fusion activity was severely affected for both µ3-1 and µ3-2, suggesting that the carbohydrate moieties at both glycosylation sites are critical for this protein function.

View larger version (38K): [in this window] [in a new window]   FIG. 5.   Further analyses of mutants µ2, µ3, and µ8. The two altered glycosylation sites in mutants µ2, µ3, and µ8 were individually mutagenized to evaluate the relative importance of each site. (a) Mutant constructs with the corresponding glycosylation site that was mutated. (b) Cell-to-cell fusion activity levels of mutants µ2-1, µ2-2, µ3-1, and µ3-2 were compared with those of the wild type, µ2, and µ3. (c) Replication kinetics of the mutants µ8-1 and µ8-2 in PBMC are shown compared to those of the wild type and µ8.

Characterization of the µ8 revertant. A characteristic property of HIV-1 mutants undergoing second-site revertant changes is the delayed appearance of progeny virus, which usually regains partial or even wild-type infection kinetics. In this study, µ2 virus harvested at the markedly delayed peak of its infection of MT-4 cells (day 20) (Fig. 4b) replicated with wild-type kinetics in subsequent infection of MT-4 cells (data not shown), indicating the very likely emergence of a µ2 revertant virus. Because of the unusual coreceptor usage phenotype of µ8 (viz., CCR5/CXCR4+) (Fig. 3), we were interested in ascertaining whether second-site revertants might have arisen during the extended µ8 infection of PBMC (Fig. 4a). Progeny virus present in day 22 culture supernatants from this infection was used to reinfect fresh PBMC. As shown in Fig. 6a, the progeny virus (designated µ8-R) replicated almost as efficiently as the wild-type virus, indicating that a reversion had, in fact, occurred. The revertant virus, µ8-R, also exhibited infection kinetics similar to that of the wild-type virus in MT-4 cells (data not shown), suggesting that the reversion permitted efficient utilization of CXCR4 as a coreceptor. In contrast, µ8-R failed to replicate in MDM (data not shown).

View larger version (37K): [in this window] [in a new window]   FIG. 6.   Characterization of second-site revertant. Replication kinetics (a) and cell-to-cell fusion activity levels (b) of the revertant µ8-R and its parental µ8 virus are compared to those of the wild type. (a) Virus replication in PBMC. CPM, counts per minute; WT, wild type.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Carbohydrate moieties on HIV-1 gp120 are known to play several important roles in the virus life cycle. Characterization of the biochemical properties of the envelope glycoproteins synthesized in the presence of various inhibitors of glycosylation or glycosidases have shown that proper attachment and trimming of glycans on gp120 are important for its folding and, therefore, for its biological function (13, 17, 19, 29, 38, 50). However, our study clearly demonstrates that gp120-associated N-linked glycosylation sites do not contribute equally during virus entry, a result consistent with previously reported site-directed mutagenesis studies for HIV-1 (25) or simian immunodeficiency virus (36). Among the glycosylation sites we have evaluated, only those that are near or within the V1/V2 (amino acids N135, N141, N156, N160) and V3 (amino acid N301) loops were critical for membrane fusion. These results are also consistent with numerous reports showing that the V1/V2 and the V3 loops are critical determinants of coreceptor usage and cellular tropism.

Although the amounts of total envelope glycoprotein expressed were quite comparable, the levels of membrane-associated gp120 were lower for some of the mutant envelopes (i.e., µ2, µ3, µ4, µ6, µ7, and µ8) compared to the wild-type protein (Fig. 2a). This could be due to decreased gp160 processing, increased shedding of gp120 from gp41, or both. Thus, the defect in fusion activity for some of the mutants could be due to the reduced levels of functional gp120-gp41 complexes on the membrane in addition to (or separate from) the inherent biochemical properties of the mutant protein. We performed our fusion assays based on the same amount of total envelope protein expressed (i.e., by using the same amount of vaccinia virus) rather than normalizing the level of membrane-associated gp120 for two reasons. First, because the altered level of membrane-associated gp120 in itself is a phenotype of the mutation, comparing different envelopes based on the same amount of total protein expressed, rather than the amount of membrane-associated gp120, would be more appropriate. Second, it is our observation that cell-to-cell membrane fusion is quite efficient and that very little envelope protein is required for this process. So, even the low amount of membrane-associated gp120 observed for some of the mutant envelopes should be more than sufficient to induce cell-to-cell fusion. For example, µ4, which has the lowest amount of membrane-associated gp120 (Fig. 2a), exhibited quite efficient fusion activity (Fig. 3). Thus, the contribution of the low level of membrane-associated gp120 on the reduced fusion activity exhibited by µ2, µ3, and µ8 is not likely to be significant.

We have identified five gp120-associated N-linked glycosylation sites that play important roles for dualtropic HIV-1_DH12. The corresponding glycosylation sites in other virus isolates may or may not serve equally important functions. For example, mutation of N301 in DH12 gp120 (sixth residue of the V3 loop) completely eliminated the usage of CCR5 and severely impaired (reducing by 50%) CXCR4 usage. Similarly, mutation of the corresponding residue on NL4-3 (T-tropic) and SF13 (dualtropic) compromised CXCR4-dependent fusion activity (34). However, no reduction of CCR5 usage was observed when the same residue was mutated on SF13 and SF162 (M-tropic) (34) or ConB (M-tropic) (51). Additionally, the corresponding mutation had no effect on infectivity of other T-tropic stains (LAI, BRU, and HXB2 [2, 27, 43]). These apparent discrepancies are likely due to specific differences in amino acid sequences in the V3 loop for different isolates, which together with carbohydrate residues create a V3 loop structure that interacts with receptors. This notion is supported by the emergence of the second-site revertant of µ8 virus (µ8-R), which acquired compensating mutations in the V3 loop without glycosylation site replacement. This revertant regained full usage of CXCR4 and replicated efficiently in PBMC and MT-4 cells, although CCR5 usage remained severely impaired. Of the two amino acid changes observed in µ8-R, a glycine-to-arginine substitution at the 11th position of the V3 loop increased the net positive charge of the V3 loop, thought to be important for CXCR4 usage (37). Coincidentally, a similar change at the 11th position of the V3 loop has been reported to rescue the defect in CXCR4 usage by a mutation affecting the glycosylation site at the 6th position of the HIV-1_NL4-3 V3 loop (34). These results indicate that depending on the amino acid residues of the neighboring region, the carbohydrate moieties may or may not play critical roles in gp120 function.

The properties exhibited by different glycosylation site mutants were not identical with respect to the extent or the type of defect. While mutating N135 (µ2-1) reduced fusion activity with both CXCR4 and CCR5 to 50% of the wild-type level, mutating N141, N156, or N160 (µ2-2, µ3-1, and µ3-2, respectively) almost completely disrupted the fusion activity with both coreceptors. Mutant µ2, which carries mutations both at N135 and N141, exhibited a phenotype similar to µ2-1. In contrast, µ8 (N295 and N301), and presumably µ8-2 (N301), exhibited preferential defect in CCR5 usage. Furthermore, unlike µ3, which exhibited reduced CD4 binding activity, mutants µ2 and µ8 interacted with CD4 as efficiently as the wild-type gp120. At present, we can only speculate about which step of the envelope glycoprotein-induced membrane fusion process is impaired. For example, the primary defect of mutant µ3, and presumably of µ3-1 and µ3-2, appears to be CD4 binding. On the other hand, µ2 seems to be impaired at one of the post-CD4 binding steps, since the mutant gp120 bound CD4 as efficiently as the wild-type protein. This defect could include the inability to undergo conformational change that occurs when gp120 binds CD4 (i.e., from closed to open conformation where the bridging sheet becomes exposed) or the inability of gp120 to bind the coreceptors. In the case of µ8, the interaction of gp120 with CCR5 is affected more than that with CXCR4. Future analyses of direct binding between different mutant gp120s and CCR5 or CXCR4 may provide additional information on the nature of the defects observed in this study.

At first glance, some of the cell-to-cell fusion and virus infectivity assay results may seem inconsistent. For example, the replication kinetics of the virus carrying the µ2 mutation was very similar to that of the wild-type virus in PBMC. In contrast, the replication of µ2 virus in MT-4 T cells was severely impaired and no evidence of its replication was detected in MDM. These results could simply reflect possible differences in the levels of CD4 and/or chemokine receptors expressed in different cell types as previously demonstrated by Ly and Stamatatos (28), who reported that mutation of glycosylation sites at the base of the V2 loop of gp120 affects viral replication kinetics in a cell-dependent manner; mutants replicated efficiently in cells expressing high levels of receptors but not in cells expressing lower levels.

Considering that recombinant vaccinia viruses express high levels of envelope glycoprotein, CD4, and coreceptors, it was somewhat surprising to observe a marked reduction in cell-to-cell fusion activity for µ2 despite its efficient replication in PBMC. A possible explanation is that both CCR5 and CXCR4 are present on primary CD4⁺ T cells while only a single coreceptor is expressed on MT-4 cells, MDM, and the M. dunni cells used for the fusion assay. The dynamics of the interactions between a dualtropic gp120 with CCR5, CXCR4, or both on the surface of primary CD4⁺ T lymphocytes remain largely unknown. Given the multiple receptor binding sites on the virion surface, the interactions between virus and cell are likely to be highly complex.