INTRODUCTION

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Several murine models have been developed for studies of AIDS pathogenesis. Mice transgenic for either the entire or partial human immunodeficiency virus type 1 (HIV-1) genome develop symptoms with similarities to AIDS. In one model, mice expressing HIV-1 Nef developed a wasting syndrome characterized by the loss of CD4⁺ cells (21, 45). In another model, SCID mice were reconstituted with human peripheral blood lymphocytes or fetal thymus and liver and then inoculated with HIV-1 (37). These have been useful for studies on mechanisms of CD4⁺ cell depletion and for evaluation of therapeutic strategies.

Current murine models lack a central feature of HIV-1-induced pathogenesis: virus replication. Inoculation of mice or rodents such as rats, hamsters, and guinea pigs with high-titer HIV-1 does not result in detectable viremia, nor does the virus replicate in murine cells in culture (37) or infect human-CD4 (hu-CD4) transgenic mice (33). Low levels of virus replication have been detected in experimentally infected rabbits (13, 15, 20, 26, 43) and cotton rats (30), but this does not induce pathogenesis. Development of a system in which HIV-1 could replicate in mice would allow the investigation of features of AIDS such as the immune response to the virus, the dynamics of virus replication, and the mechanism of T-cell depletion. It would also make available the wide range of serological reagents and the well-characterized genetics in the murine system.

HIV-1 fails to replicate in murine cells for several reasons, the best studied of which is the block to virus entry. The HIV-1 envelope glycoprotein (Env) binds with high affinity to hu-CD4 but does not bind measurably to murine (mu-CD4) (29). In addition, HIV-1 Env fails to interact with mu-CCR5 (2). Unexpectedly, some T-tropic HIV-1 Env proteins are able to use mu-CXCR4 for infection (5, 48). Coexpression of transfected hu-CD4 and hu-CCR5 or hu-CXCR4 in murine cells in culture allows the entry of HIV-1 (4). Alternatively, the entry block can be circumvented by pseudotyping HIV-1 with amphotropic Moloney murine leukemia virus Env or vesicular stomatitis virus G (VSV-G), which allows entry through different receptors. In mice transgenic for hu-CD4 and hu-CCR5 (8) or hu-CXCR4 (44), inoculation with HIV-1 resulted in a small number of infected cells but no detectable viremia. In contrast, rabbit cells expressing transfected hu-CD4 and hu-CCR5 are permissive for robust HIV-1 replication (47).

In addition to the entry block in murine cells, HIV-1 long terminal repeat (LTR)-directed transcription is inefficient in murine cells due to weak Tat activity in rodent cells (1). Using transfected reporter constructs, transactivation of LTR transcription by Tat was 10- to 25-fold less active in rodent cells. The deficiency in Tat function in rodent cells could be complemented in rodent/human somatic cell hybrids containing human chromosome 12 (1, 23, 38). The active gene was recently identified as cyclin T1 (51), a partner for the cyclin-dependent kinase CDK9 in the transcription elongation factor P-TEFb (19, 34, 55). Cyclin-T1/CDK9 binds the TAR transactivation region of nascent HIV-1 transcripts, promoting hyperphosphorylation of serine and threonine residues in the cytoplasmic tail domain of RNA polymerase II (reviewed in reference 17). Phosphorylation increases the processivity of the transcription complex, allowing the synthesis of the full-length HIV-1 primary RNA transcript. Mu-cyclin T1 is competent to bind Tat, but the resulting complex fails to bind TAR, a phenotype that results from the presence of a tyrosine in place of cysteine at residue 261 (7, 18, 27). Transfection of a human cyclin T1 (hu-cyclin T1) expression vector restored Tat function in rodent cells in an HIV-1 LTR reporter vector assay and in infected murine cells (7, 18, 27, 51).

Murine NIH 3T3 cells expressing transfected hu-CD4, hu-CCR5, and hu-cyclin T1 failed to support HIV-1 replication (18). While the cyclin T1 allowed for more efficient expression of the HIV-1 provirus, infected cells produced only low levels of p24^gag, suggesting the presence of additional blocks to HIV-1 replication in the murine cells. Here we investigated the basis of this additional block in the murine cells by measuring the efficiency of each step of virus replication in NIH 3T3 reporter cells expressing these three human cofactors. Consistent with earlier findings (18), the murine cells were not permissive for HIV-1 replication; however, they efficiently supported several of the steps in the virus life cycle. Virion structural proteins were expressed at high level but, instead of assembling at the cell membrane, were trapped in cytoplasmic vesicular structures. As a result, Gag was poorly processed and virions were released only at low levels. Interestingly, the small amount of Gag protein that was released from the cells was in the form of infectious virus, suggesting that the block to replication in murine cells is not absolute.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Derivation of murine and human reporter cell lines. Murine reporter cells were derived by transfecting NIH 3T3 cells with plasmid pLTR-EGFP-1, which contains the HIV-1 LTR linked to the enhanced green fluorescent protein (EGFP) gene. Transfectants were selected in medium (Dulbecco's modified Eagle's medium plus 10% fetal bovine serum) containing 0.4 mg of G418 per ml. The resulting drug-resistant population was sorted by fluorescence-activated cell sorting (FACS) for those expressing low but detectable green fluorescence. The sorted cells were then infected with pBABE-CCR5 retroviral vector stock and selected 2 days later in medium containing 0.2 µg of puromycin per ml. Puromycin-resistant cells were sorted by FACS for those that stained with the anti-CCR5 monoclonal antibody 2D7 (Pharmingen). The cells were then infected with the retroviral vector pMX-CD4, based on the vector pMX (39), that contains hu-CD4 but no selectable marker. CD4 expressing cells were selected on anti-CD4-coated magnetic beads (Dynal) and were plated at limiting dilution. Individual clones were picked and analyzed for high CD4 expression. One clone that expressed uniformly high levels of CD4 was arbitrarily chosen and named MGT5 for murine/GFP/T4/CCR5. MGT5 cells were infected with the hu-cyclin T1 retroviral expression vector pBABE.cyT(hygro) based on the retroviral vector pBABE.hygro (36). The infected cells were selected in medium containing 200 µg of hygromycin per ml. Individual drug-resistant clones were expanded and analyzed for their response to transfected Tat expression vector. A clone was chosen and named MGT5.cyT for murine/GFP/T4/CCR5/cyclin T1.

Viruses and infections. NL4-3(VSV-G) replication-competent pseudotypes were produced by transfecting 293T cells with an equal amount of pNL4-3 and VSV-G expression vector. Culture supernatants were harvested 48 h postinfection, filtered, and frozen at 80°C in aliquots. Luciferase reporter viruses were produced by cotransfecting 293T cells with NL-Luc-ER and Env expression vectors for JR.FL or VSV-G as previously described (12). For dual luciferase infections, the NL-Luc RE vector (10) was modified by replacing the firefly luciferase gene with that Renilla luciferase gene (Promega). Cultures were infected with an equal mixture of the two different reporter viruses, and each virus was quantitated independently using dual luciferase reporter assay reagents (Promega). Viruses were quantitated by p24^gag enzyme-linked immunosorbent assay (ELISA).

Quantitative-competitive PCR quantitation of proviral copy number. Genomic DNA was prepared from 10⁶ murine and 10⁶ human cells 3 days postinfection and then cleaved with SphI to decrease its viscosity. Aliquots of genomic DNA (1 µg) were mixed with decreasing quantities of competitor pNL4-3 containing a 1.4-kb deletion in env from the NheI to HpaI sites (nucleotides [nt] 6628 to 8028). The mixture was amplified by PCR using primers RC-9 and RC-12 (11), which flank env. Amplified proviral and competitor plasmid were separated by electrophoresis through 1% agarose, visualized by ethidium bromide staining, and quantitated by densitometry.

Northern blotting. Total cellular RNA was isolated from cells infected 3 days earlier with NL4-3(VSV-G) using Triazol (Life Technologies). RNA (10 µg) was separated by electrophoresis on an agarose-formaldehyde gel and transferred to a nylon filter. The filter was hybridized to an [-³²P]dCTP-labeled HIV-1 LTR probe derived from the XhoI-BglII fragment (nt 8265 to 8419) of HIV-1_SF2. The filters were exposed to film and subsequently quantitated using a PhosphorImager and ImageQuant software (Molecular Dynamics). The filter was then stripped and reprobed with an antisense oligonucleotide to glyceraldehyde phosphate dehydrogenase mRNA (nt 1017 to 1037).

Western blotting. Cells were infected with NL4-3(VSV-G), and 3 days later virions and cell lysates were prepared. Virions were pelleted by centrifuging 10 ml of filtered culture supernatant at 35,000 rpm in an SW40Ti rotor for 1 h and lysed in lysis buffer (100 mM NaCl, 10 mM EDTA, 20 mM Tris [pH 7.5], 1% Triton X-100, 1% sodium deoxycholate). Cell lysates were prepared by removing medium from the infected cells, washing them with phosphate-buffered saline, and lysing them in 500 µl of lysis buffer. Protein in the lysates was quantitated by using Coomassie blue reagent (Bio-Rad) and stored at 80°C before use. Lysate containing 10 µg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride filters, and probed with serum from an AIDS patient (1:500 dilution; gift of D. Richman, University of California San Diego) followed by horseradish peroxidase-conjugated rabbit anti-human antibody (BioSource International). Filters were developed using luminescent enhanced chemiluminescence reagents (Amersham) and quantitated by using a PhosphorImager.

Density gradient centrifugation of virions. Supernatants (3 ml) from murine and human cells that had been infected 3 days earlier at high multiplicity of infection (MOI) with NL4-3(VSV-G) were overlaid on 20 to 60% linear sucrose gradients. The gradients were centrifuged at 35,000 rpm in an SW40T: for 12 h and fractionated into 10, 0.9-ml fractions. The density of each fraction was determined by refractometry. The fractions were then diluted with 2 volumes of TNE (10 mM Tris [pH 7.5], 100 mM NaCl, 1 mM EDTA) and centrifuged for 1 h at 35,000 rpm in an SW55Ti rotor. Pelleted virions were resuspended in 100 µl of lysis buffer. p24^gag was quantitated in the fractions by ELISA.

Electron microscopy. MGT5 and MGT5.cyT cells were seeded on large glass coverslips in 10-cm dishes (10⁶ per dish) and 1 day later were infected with NL4-3(VSV-G) at a MOI of 0.5. The cells were fixed 72 h postinfection in situ for 20 min with cold 2.5% glutaraldehyde in 100 mM PIPES (piperazine-1,4-bis-2-ethanesulfonic acid [pH 6.9]). Fixed cells were scraped from the coverslip with a rubber policeman, collected by low-speed centrifugation at 4°C, washed three times in cold 100 mM PIPES, and postfixed in 1% osmium tetroxide in PIPES for 30 min at 4°C. After extensive washing in 100 mM PIPES, the cells were embedded in low-melting-temperature agarose (Sigma) and further treated with 1% tannic acid for 10 min. Finally, agar blocks were dehydrated in ethanol and embedded in ERL resin. Silver-gray sections were stained with lead citrate and uranyl acetate. The sections were examined in a Philips CM120 electron microscope at 60 kV.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Expression of hu-CD4, hu-CCR5, and hu-cyclin T1 in murine cells allows for HIV-1 infection but not replication. To analyze the blocks to HIV-1 replication in murine cells, we derived reporter cell lines MGT5 and MGT5.cyT, based on NIH 3T3 fibroblasts (Fig. 1A). MGT5 was established by first introducing an HIV-1 LTR-EGFP cassette into NIH 3T3 cells and then transducing hu-CCR5 and hu-CD4 with retroviral vectors. Individual cell clones were evaluated by flow cytometry, and a clone with low-level constitutive green fluorescence and uniform CCR5 and CD4 expression was chosen. hu-cyclin T1 was introduced into MGT5 by retroviral vector transduction, resulting in MGT5.cyT. These cells are the murine equivalents of GHOST.R5 (24), a HOS-derived cell line that expresses CD4 and CCR5 and contains LTR-EGFP. The murine cell lines expressed cell surface CD4 at levels approximating those of GHOST.R5 (Fig. 1B). hu-CCR5 was expressed at a slightly higher level in MGT5.cyT than in MGT5, possibly due to a transcriptional effect of cyclin T1. Expression of cyclin T1 could not be directly quantitated in these cells because of the presence of the endogenous murine cyclin T1, but it was verified by Western analysis in NIH 3T3 cells expressing HA-tagged cyclin T1 in cells prepared in parallel (data not shown). Although fibroblasts are not natural targets of HIV-1 in vivo, NIH 3T3 cells support HIV-1 reverse transcription and integration (28, 40) and, when transfected with hu-CD4 and CCR5 or CXCR4, support entry (3). In contrast, lymphoid cells from hu-CD4/CXCR4 transgenic mice were poorly infectable by reporter virus (data not shown). Moreover, human fibroblastic cell lines, such as HOS.CD4.CCR5 or HeLa.CD4, are highly permissive for HIV-1 replication, indicating the ability of HIV-1 to replicate efficiently in nonlymphoid cells once the entry block is removed.

View larger version (21K): [in this window] [in a new window]   FIG. 1.   Derivation of MGT5 and MGT5.cyT reporter cells. (A) NIH 3T3 cells were transfected with pLTR.EGFP, which contains the HIV-1 LTR linked to the EGFP gene. hu-CD4, hu-CCR5, and hu-cyclin T1 were introduced using pBABE retroviral vectors (36). MGT5.cyT was transduced with BABE.CyT.hygro; MGT5 contains control vector BABE.hygro. (B) FACS analysis of CCR5 and CD4 on MGT5, MGT5.cyT, GHOST.R5, and NIH 3T3 cells.

View larger version (2332K): [in this window] [in a new window]   FIG. 2.   Cyclin T1 restores HIV expression but not replication in the murine cell line. (A) Murine and human cell lines were infected with 25 ng of p24gag NL4-3(VSV-G) and photographed under UV light 48 h later. Uninfected cells are shown below. (B) Murine and human cells were infected with the amounts of NL4-3(VSV-G) p24gag indicated on the right. The percentage of cells within the gate defined by the vertical line in each panel is indicated. (C) MGT5, MGT5.cyT, and GHOST.R5 cells were infected with M-tropic HIV-1 isolates JR.FL and MJM at an MOI of 0.05, and p24gag production was measured for 14 days. Continuation of the cultures for up to 4 weeks did not result in virus production from the murine cells (data not shown).  FIG.2Continued.

Efficient HIV DNA synthesis and integration in the murine cells. To evaluate the ability of the murine cells to support HIV-1 DNA synthesis and integration, viral DNA in the infected murine and human cells was measured by quantitative-competitive PCR. Latently infected OM10.1 cells that harbor a single HIV-1 provirus were used for standardization. GHOST.R5 cells contained a proviral load similar to that of OM10.1. The MGT5 and MGT5.cyT provirus loads were about twofold lower (Fig. 3). It was not clear whether the slightly increased amount of virus DNA in the GHOST.R5 cells was due to a small increase in DNA synthesis or to experimental variability. Although the GHOST.R5 cells contained more viral DNA than the murine cells, they did not show a correspondingly greater proportion of fluorescent cells. This could indicate that the murine cells expressing hu-cyclin T1 are more efficient at activating the integrated LTR-EGFP than are the human cells. GHOST.R5 contains an HIV-2 LTR-EGFP, which may be less responsive to HIV-1 Tat than is the HIV-1 LTR-EGFP in MGT5 cyT. In this analysis, the DNA measured is likely to represent integrated provirus, since unintegrated forms tend to be rapidly lost (41), although some contribution of unintegrated circles cannot be excluded. Taken together, these data suggest that the murine cells support reverse transcription and integration with an efficiency similar to that of highly permissive human cells and that cyclin T1 affected proviral expression but not any other early steps in virus replication.

View larger version (19K): [in this window] [in a new window]   FIG. 3.   Murine cells support efficient HIV-1 provirus formation. Cells were infected with NL4-3(VSV-G) at an MOI of 1. Genomic DNA was prepared 2 days later, and proviral copy numbers were determined by quantitative-competitive PCR. Competitor DNA deleted between the NheI and HpaI sites flanking env was added in the amounts indicated above each lane. DNA from OM10.1 cells containing a single provirus per cell was used for calibration. The proviral load per cell calculated from band intensities normalized to OM10.1 was as follows: GHOST.R5 = 1, MGT5 = 0.5, and MGT5.cyT = 0.25.

Murine cells synthesize and splice HIV-1 RNA. In HIV-1-infected cells, the quantity of HIV-1 mRNA is controlled by the LTR and by Tat while the characteristic pattern of fully spliced, partially spliced, and unspliced viral mRNA transcripts is regulated by Rev (reviewed in reference 42). While the intensity of EGFP fluorescence in the infected MGT5.cyT cells suggested that HIV-1 RNA had been synthesized, this was not indicative of Rev activity, since the LTR-EGFP was activated by Tat, which is synthesized from a Rev-independent, fully spliced transcript. To evaluate the production of HIV-1 mRNA transcripts in murine cells, HIV-1 transcripts in the NL4-3(VSV-G)-infected cells were visualized by Northern blotting. At 3 days postinfection, the murine MGT5.cyT cells were found to contain the characteristic unspliced, singly spliced, and multiply spliced mRNA transcripts (Fig. 4), although at a slightly altered ratio. The proportion of the unspliced transcript was somewhat reduced compared to that in the human cells (unspliced/multiply spliced ratio of 11.4 in GHOST.R5 and 5.0 in MGT5.cyT), and the relative amount of unspliced transcript was about threefold lower in the murine cells. Real-time PCR quantitation of these transcripts confirmed these results (data not shown). Thus, Rev function was intact in the murine cells. The reduction in unspliced transcript could have been due to a small reduction in Rev activity in the murine cells or to more rapid degradation of the long transcript. Transfecting an expression vector for the Rev cofactor Crm-1/exportin-1 (42) did not alter the ratio of transcripts in the murine cells (data not shown). In addition, while the human cells produced threefold more HIV-1 mRNA transcripts than did the murine cells, they contained two- to fourfold more proviruses. Thus, on a per provirus basis, the murine cells produced mRNA transcripts at a level comparable to that of the human cells.

View larger version (80K): [in this window] [in a new window]   FIG. 4.   Murine cells produce unspliced and spliced HIV-1 transcripts. Murine and human cells (2 × 106) were infected with NL4-3(VSV-G) at an MOI of 1. At 2 days postinfection, total RNA was prepared and HIV RNA transcripts were detected by Northern blotting using an LTR probe. Unspliced (US), singly spliced (SS), and multiply spliced (MS) transcripts are indicated by arrows. The filter was reprobed with a glyceraldehyde 3-phosphate dehydrogenase (GAPDH) probe to control for equal RNA loading. The ratio of unspliced to multiply spliced transcripts was 11.4 for GHOST.R5 and 5.0 for MGT5.cyT; the ratio of intensities of the unspliced transcript GHOST.R5 compared to MGT5.cyT was 3.0 and for multiply spliced it was 1.3, as determined by PhosphorImager analysis.

Murine cells synthesize and process HIV-1 proteins but fail to release virions efficiently. Production of viral proteins by the NL4-3(VSV-G)-infected murine and human cell lines was evaluated by Western analysis of cell lysates and supernatants. This analysis showed that the infected GHOST.R5 and MGT5.cyT cells contained readily detectable amounts of HIV-1 structural proteins (Fig. 5). Lysates of GHOST.R5 and MGT5.cyT contained Gag polyproteins pr55^gag and pr160^gag/pol, the Gag-processing intermediate pr41, and fully processed Gag proteins p24 CA and p17 MA. The presence of pr160^gag/pol at an abundance similar to that of GHOST.R5 suggested that the translational frameshifting event required for synthesis of the Gag-Pol polyprotein occurred in the murine cells at a frequency comparable to that in human cells, consistent with an earlier report by Moosmayer et al. (35). Importantly, while the pr55^gag polyprotein was present at a similar abundance in the murine and human cells, fully processed CA was significantly reduced in abundance in the murine cells. Quantitation of the band intensities showed that pr55^gag was actually present at slightly higher level in the MGT5.cyT than in the GHOST.R5 cells but that CA was reduced 7.7-fold in the murine cells (Table 1). In GHOST.R5, CA was about 46% as abundant as pr55^gag, while in MGT5.cyT, CA was about 5% as abundant.

View larger version (80K): [in this window] [in a new window]   FIG. 5.   HIV-1-infected murine cells synthesize viral proteins but fail to efficiently release virions. Murine and human cells were infected with NL4-3(VSV-G) at an MOI of 1. At 3 days later, virions were pelleted from the culture medium and cell lysates were prepared. Viral proteins in the virions and cell lysates were visualized on a Western blot probed with patient serum. For the lysates, a fixed amount of protein from the murine cells and 10-fold-decreasing amounts of protein starting at 5 µg from the human cells was loaded. For the virions, amounts corresponding to 1.0 or 0.1 ml were loaded, as indicated above the lanes.

View larger version (33K): [in this window] [in a new window]   FIG. 6.   Infected murine cells contain Gag at levels sufficient to allow virion assembly. (A) OM10.1 cells were treated with 5.0, 1.0, 0.2, 0.02, 0.01, 0.005, or 0 U of TNF- per ml in the lanes below the triangle. GHOST.R5 and MGT5.cyT were infected with NL4-3(VSV-G), and lysates and virions from each culture were prepared 3 days later. Viral proteins were analyzed on Western blots with AIDS patient serum. (B) p24gag concentration in lysates and virions.

Murine cells produce small numbers of infectious virions. Although the murine cells released only small amounts of p24^gag, it remained possible that this was in the form of infectious virions. To test whether the p24^gag had been released from the cells in the form of virions, supernatants from NL4-3(VSV-G)-infected MGT5.cyT cells were subjected to sucrose density gradient analysis (Fig. 7A). Supernatant, without pelleting, was layered on a sucrose density gradient and centrifuged to equilibrium. Analysis of gradient fractions showed that p24^gag banded at 1.14 g/ml, a density consistent with that of virions and similar to the density of virions produced by the human cell line 293T. Similar results were obtained with GHOST.R5 cells, but these were less efficient at producing virions than were the 293T cells (data not shown). A high-density peak was present in the 293T-derived gradient, possibly corresponding to aberrantly formed virions secreted by these cells.

View larger version (26K): [in this window] [in a new window]   FIG. 7.   Murine cells produce infectious virus. (A) Murine and human cells were infected with NL4-3(VSV-G). At 3 days postinfection, the density of virions in the supernatant was measured by sucrose density gradient centrifugation. (B) p24gag was quantitated in cell lysates and from pelleted virions. (C) The infectious titer (TCID50) of supernatant virus was measured by end-point dilution on CEM.LTR.GFP cells. The ratio of TCID50 to supernatant p24gag is 154 and 236 for the MGT5.cyT- and GHOST.R5-produced viruses, respectively.

Aberrant structures accumulate in vesicles. Electron microscopic analysis of the infected MGT5.cyT cells showed rare, budding structures at the plasma membrane that were of normal size and morphology (Fig. 8C). Such structures were not observed in infected MGT5 cells or uninfected controls. A few extracellular immature (Fig. 8D) and mature (Fig. 8E) particles were detected near the plasma membrane of infected MGT5.cyT cells but not in uninfected or MGT5 cells. The virions were of typical size and appearance, and the characteristic cone-shaped internal core structure of mature HIV-1 virions was apparent in a small number of instances (Fig. 8E). No budding structures at intracellular membranes and no cytoplasmic or vesicle-associated capsids or virus-like particles were observed.

View larger version (179K): [in this window] [in a new window]   FIG. 8.   Ultrathin-section electron microscopic analysis of infected MGT5.cyT cells. (A) Overview of an infected MGT5.cyT cell. (B) Detail of an infected cell, revealing a complex vesicular cytoarchitecture. Note the thickened contrast-rich membranes situated in close association with cisternae of the endoplasmic reticulum (arrowheads). Adjacent is a dense body (D), also surrounded by a thickened membrane. (C to E) Budding structures and immature and mature HIV-1 particles, respectively.

CCR5 and CD4 on the murine cells mediate entry with reduced efficiency. In the experiments described above, VSV-G pseudotypes that bypass CD4/coreceptor-mediated entry were used to achieve efficient infection. To evaluate the efficiency of CD4/coreceptor-mediated entry in the murine cells, MGT5.cyT and GHOST.R5 cells were infected with a mixture of two luciferase reporter viruses: CCR5-specific JR.FL pseudotyped firefly luciferase reporter virus and VSV-G pseudotyped Renilla firefly reporter virus (in which the firefly reporter gene was replaced by Renilla luciferase). This procedure allowed the measurement of both viruses in dually infected cultures, permitting normalization for any postentry differences between the cell types. In GHOST.R5 cells, the JR.FL pseudotype induced luciferase activity close to (about 75%) that of the VSV-G pseudotype (Fig. 9A). In MGT5.cyT cells, JR.FL entry was less efficient than that of VSV-G (about 20%). This difference was not due to postentry differences between the two cell lines, since the VSV-G pseudotype was used to normalize for such differences. Nor was the difference due to limiting amounts of CD4 or CCR5, since these were expressed in ample amounts in the murine cells, as shown above (Fig. 1B). Thus, hu-CD4/hu-CCR5 on the murine cells mediated HIV-1 entry but at significantly reduced efficiency with JR.FL Env.

View larger version (25113K): [in this window] [in a new window]   FIG. 9.   Efficient syncytium formation but decreased coreceptor-mediated entry in the murine cell line. (A) The murine and human cells were infected with a mixture of JR.FL pseudotype containing a firefly luciferase reporter gene and VSV-G pseudotype containing Renilla luciferase reporter gene. The activities of the two luciferase enzymes were quantitated using reagents discriminating between the two enzymes. Data are reported as the ratio of JR.FL- to VSV-G-mediated infection (firefly divided by Renilla luciferase activity in counts per second). (B) Syncytium formation assay in which 293T cells expressing transfected JR.FL Env were cocultured for 18 h with MGT5 or GHOST.R5. Representative fields are shown.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Murine NIH 3T3 fibroblasts stably expressing hu-CD4, hu-CCR5, and hu-cyclin T1 fail to support HIV-1 replication primarily because of a block to virus assembly. Interestingly, the cells supported the other steps of the HIV-1 replication cycle. Reverse transcription/integration, proviral transcription, protein synthesis, and frameshifting were nearly as efficient as in human cells, while production of unspliced HIV-1 mRNA transcripts and CD4/CCR5-mediated virus entry were somewhat reduced. In contrast, the block to virus assembly was dramatic. Upon infection with NL4-3(VSV-G) pseudotypes, the murine cells produced large amounts of Gag and Gag-Pol, but this accumulated in the cells in vesicular structures and was largely not released. The Gag proteins were inefficiently processed by the viral protease, probably as a result of their failure to assemble, consistent with earlier findings in transfected rodent cells (35). Importantly, the infected murine cells produced small amounts of virions, and these were as infectious on a per-particle basis as those from human cells, consistent with findings by Garber et al. (18), who used transfected NIH 3T3 cells.

Provirus expression, which is nearly undetectable in NIH 3T3 cells, was largely restored by expressing hu-cyclin T1, consistent with earlier findings (18). The small reduction in LTR-EGFP activation in the infected MGT5.cyT cells could have been caused by competition from the endogenous mu-cyclin T1, which is thought to have dominant negative activity on the human protein (6, 27), or to inefficient LTR function resulting from incompatibilities between murine transcription factors and LTR binding-site sequences. We could not distinguish between these two possibilities. Decreased Tat activity does not appear to account for the failure of the virus to replicate in MGT5.cyT cells, since upon infection, the cells produced ample amounts of virion structural proteins.

An earlier report (49) suggested that there is a block to Rev function in murine cells; however, this did not appear to be the case in our study. Judging by the ratio of spliced to unspliced HIV-1 mRNA transcripts and indirectly by the accumulation of viral structural proteins which are translated from Rev-dependent mRNA transcripts, Rev function was largely intact in the murine cells. A threefold reduction in the relative abundance of the unspliced transcript in the murine cells was noted, perhaps reflecting a small reduction in Rev activity; however, this could also be caused by some instability of the unspliced transcript, which, because of its failure to be encapsidated by assembling virions, may be more rapidly degraded by cellular RNase. Transfecting the murine cells with Crm-1/exportin-1 expression vector, the human Rev cofactor (42), did not alter viral protein production or mRNA transcript ratios (data not shown).

While some steps of the virus replication cycle were somewhat less efficient in the murine cell line (mRNA synthesis and virus entry), the block to assembly was drastic. Although the murine cells contained amounts of Gag polyprotein at levels comparable to that in the human cells, the levels of p24^gag released were more than 500-fold reduced. The reason for the failure to assemble virions is unknown. Processing of the Gag polyprotein by the viral protease was inefficient; however, this was more likely to have been a consequence of the failure of the polyprotein molecules to assemble than its cause, since processing is not required for budding (16). In addition, it was not likely to have been due to a failure to incorporate cyclophilin, since incorporation of this cellular protein is not required for assembly or budding (53). Nor was it due to an inappropriate association of Env with Gag at the cell membrane, since cells infected with Env NL4-3(VSV-G) also failed to assemble virions (data not shown).

The inability of the murine cells to support HIV-1 assembly is presumably caused either by the absence of a required human-specific host factor or by the presence of an inhibitory murine factor. HIV-1 assembles at the cell membrane by a process similar to that of the type C retroviruses (16). The Gag polyproteins are transported to the cell membrane in an energy-dependent process (31) that may be mediated by vesicles (22, 25) and that is directed by specific domains of MA. MA contains an N-terminal basic patch and an N-terminal myristate that are required for membrane attachment (16, 46, 54). Deleting a portion of MA results in targeting of viral assembly to intracellular membranes (14). Adding a src membrane-targeting sequence to an intracisternal A-type particle Gag redirected it to assemble at the cell membrane, release, and process (52). In the infected murine cells, inappropriate targeting of the Gag polyprotein could have caused the accumulation of virion proteins in vesicular structures that we observed by electron microscopy. Targeting to the cell membrane could involve cellular cofactors, such as those that mediate protein folding or intracellular transport, any of which could be altered or lacking in the murine cells. Using an in vitro assembly system, Lingappa et al. (31) detected at least two host cofactors, one detergent sensitive and the other insensitive, that were required for the assembly of HIV-1 capsids. Whether these activities are present in murine cells is not known. In addition, the chaperonin T-complex polypeptide has been implicated as playing a role in hepatitis B virus assembly (32). A similar factor could play a role in HIV-1 assembly.

Alternatively, murine cells might contain an inhibitor of virus assembly. The expression of variant HIV-1 Gag molecules in human cells interferes with the production of infectious particles (50). By analogy, endogenous murine retroviral Gag proteins or fortuitous interaction of the Gag polyprotein with murine cellular proteins could interfere with assembly. Because the virus has evolved to replicate in human cells, it would not have been selected to avoid interference by murine cellular proteins. Our data do not allow us to distinguish between the two types of models; however, supporting the former hypothesis, Trono and Baltimore found that fusing a human cell line to HIV-1-infected NIH 3T3 cells boosted the release of p24^gag (49).

The finding that the murine cells produced virions with an infectivity similar to that of virions from human cells indicates that there is no absolute block to HIV-1 replication in murine cells. The failure to support replication probably results from the combination of insufficient numbers of infectious particles released and decreased entry efficiency. Conceivably, long-term adaptation of the virus to growth in murine cells could select for a rapidly growing variant; however, this did not occur following culturing of MGT5.cyT with replication-competent M-tropic HIV-1 for more than 4 weeks (data not shown). Establishing a murine model for HIV-1 replication will require more extensive alteration to the virus than that which occurs during virus replication or will require identification of the factors that mediate HIV-1 assembly.