INTRODUCTION

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Human cytomegalovirus (HCMV) is a ubiquitous betaherpesvirus that is found in over 80% of the population. While asymptomatic in most immunocompetent hosts, in immunocompromised individuals, such as transplant recipients and AIDS patients, HCMV causes a wide range of clinical symptoms which, if left untreated, are often fatal (6, 17). Currently, patients are treated with antiviral drugs, such as ganciclovir and Foscarnet, which inhibit HCMV-permissive infection (33, 37, 40). However, with the dramatic rise in immunocompromised individuals who require long-term antiviral treatment, drug-resistant strains of HCMV are becoming more common, resulting in a loss of ability to control infection (reviewed in reference 12). This problem has resulted in the need to identify and characterize new antiviral targets which can be used to inhibit the life cycle of HCMV.

Several reports have demonstrated that HCMV infection induces activation of numerous host cell transcription factors such as Sp-1, CREB/ATF family members, and NF-B (7, 20-22, 30, 56-58). This activation ensures high levels of expression of the many viral and cellular genes which are required for completion of the lytic life cycle. Since many of these transcription factors are required for expression of certain genes, and hence are necessary for completion of the viral life cycle, inhibiting their activation represents one mechanism to inhibit viral infection.

One way to inhibit the activation of cellular transcription factors may be to inhibit upstream signaling events which control their activity. Since the transactivation function of many cellular transcription factors is at least partially regulated by phosphorylation events, identifying and inhibiting cellular kinases which phosphorylate transcription factors may represent one mechanism to inhibit HCMV permissiveness (2, 49). This has led members of our laboratory and others to search for specific kinase pathways which are activated following HCMV infection and which activate transcription factors. Mitogen-activated protein kinases (MAPKs) are examples of kinases which activate numerous transcription factors, and some members of the MAPK family are strongly activated following HCMV infection (19, 46).

MAPKs are important cellular signaling kinases which are activated by dual phosphorylation on specific tyrosine and threonine residues in response to various external and internal stimuli (reviewed in references 10 and 45). In mammalian cells, three general groups of MAPKs have been identified: extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38/Hog. Each MAPK is positioned at the bottom of a distinct kinase pathway composed of three sequential dual-specificity kinases, generically termed the MAPKKK (MKKK or MEKK), MAPKK (MKK or MEK), and MAPK. Following stimulation, the MKKK is dually phosphorylated on specific residues by cellular signaling molecules. This activated MKKK then phosphorylates the MKK. Once activated, the MKK phosphorylates the MAPK on the appropriate threonine and tyrosine residues, resulting in MAPK activation (10, 45).

Activated MAPKs can phosphorylate numerous substrates, including a variety of transcription factors (2, 28, 52). In the case of transcription factors, MAPK-mediated phosphorylation is a common mechanism utilized by the cell to induce gene expression (28, 52). Largely through their ability to regulate transcription factors, MAPKs regulate changes in the cell ranging from cell growth to apoptosis to senescence.

Because of the many effects of MAPK signaling on cell growth and viability, the level of phosphorylated, and hence active, MAPK is tightly regulated by the cell. Phosphorylation of MAPK is determined by two processes, MKK kinase activity and phosphatase activity. In the absence of stimulation, there is a low level of basal MKK kinase activity, which phosphorylates the MAPK. This basal activity is counteracted by an equal low level of basal MAPK phosphatase activity. This balance prevents an accumulation of activated MAPK in the absence of stimulation. Following stimulation, MAPK is dramatically activated (by phosphorylation via the MAPK pathway). In many instances, shortly after MAPK activation, MAPK phosphatases are activated. However, the high levels of MKK activity prevents a decrease in the amount of activated MAPK. Once the upstream signal subsides, MKK activity drops, and the MAPK is quickly dephosphorylated by the activated phosphatases (reviewed in reference 16).

Numerous viruses activate one or more of the MAPKs. For example, simian virus 40 (SV40) activates ERK1/2, herpes simplex virus (HSV) activates both JNK and p38, and simian immunodeficiency virus activates all three MAPKs (34, 42, 54, 59). In addition, MAPK activation can be induced by viral binding to the host cell, such as is the case with simian immunodeficiency virus activation of ERK1/2, JNK, and p38, or it can require viral protein synthesis, as is the case with HSV activation of JNK (42).

While there are many reports which demonstrate MAPK activation following viral infection, few have determined whether this activation is important for a viral permissive infection. The recent identification of dominant negative proteins and chemical compounds which specifically inhibit different MAPK kinases have allowed more detailed analysis of the function of MAPK activation in virally infected cells (26, 27, 44). Using 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl) 1 H-imidazole (FHPI), a drug which inhibits p38 kinase activity, investigators demonstrated that p38 kinase activity was necessary for interleukin-1-mediated increased in human immunodeficiency virus replication (11), as measured by increases in pp24 levels (27, 48). In another study, dominant negative proteins were used to demonstrate that inhibition of HSV-mediated JNK activation significantly decreases viral titers (34). However, even though p38 activation was observed following HSV infection, FHPI had no effect on viral titers, indicating that not all MAPKs which are activated following infection have a critical role in viral permissive infection (T. I. McLean and S. L. Bachenheimer, personal communication).

Even in cases where it has been determined that MAPK activation has a function in viral infection, little is known about how MAPK mediates this effect. For example, MAPK substrates which are activated following viral infection have not been identified. Furthermore, little is known about cellular proteins that are involved in virus-mediated MAPK activation. An understanding of these points is of great interest since it will allow investigators to better examine the antiviral potential of MAPKs, in addition to greatly increasing our knowledge of how the virus regulates host cell machinery to ensure a permissive infection.

Interestingly, HCMV infection has been found to activate both ERK1/2 and p38. In the absence of prestimulation, ERK1/2 activation is observed at 5 to 15 min following viral binding to the cell (3) and at 4 to 8 h postinfection (hpi) following viral gene expression (46). Increased p38 activity is detected at 8 hpi and maintained through 48 hpi (19).

Excitingly, treatment of infected cells with drugs which inhibit either ERK1/2 activation or p38 kinase activity significantly delays viral DNA replication and subsequent plaque formation (19; R. A. Johnson, A. D. Yurochko, S.-M. Huong, and E.-S. Huang, unpublished data). Additional studies have shown that inhibition of either p38 or ERK1/2 kinase activity does not affect expression of the two viral major immediate-early (IE) genes, IE1-72 and IE2-86 (19, 46). However, while inhibition of ERK1/2 activation reduced expression of several viral E genes necessary for initiation of viral DNA replication, inhibition of p38 kinase activity did not, suggesting that rather than having redundant roles, each has a distinct function in HCMV infection (19, 48; Johnson et al., unpublished data).

As with other viruses, little is known about the mechanism of p38 activation following HCMV infection or which cellular proteins are phosphorylated in a p38-dependent manner following infection. Therefore, we have undertaken a detailed study into the mechanism of p38 activation following HCMV infection. Initially, we provide evidence that p38 is not activated immediately following viral binding to the host cell. Rather, p38 is first activated at 8 to 10 hpi, and this activation is maintained throughout infection (72 hpi), though a decrease in activity is often detected at 24 hpi. Since extended activation of p38 is unusual, we next examined whether any p38 substrates were also activated for extended periods of time in a p38-dependent manner. We present data suggesting that activation of p38 for extended periods of time is necessary for HCMV-mediated hyperphosphorylation of pRb, which is observed at 48 to 72 hpi, and for phosphorylation of heat shock protein 27 (HSP27), which is observed from 12 to 48 hpi.

We then focused on the mechanism by which HCMV-mediated this unusually long-term activation of p38. We provide evidence that HCMV utilizes two mechanisms to activate p38. Our results suggest that at early times of infection, HCMV inhibits dephosphorylation of activated p38, allowing an accumulation of phosphorylated p38 in the absence of increased MKK3/6 activity. At later times of infection (48 to 72 hpi), increased activation of MKK3/6 allows continued p38 activation. To our knowledge, this is the first report of any stimulus or virus which activates p38 by inhibiting dephosphorylation, underscoring the complex interaction between HCMV and infected host cells.

Collectively, the results of this study demonstrate that p38 is activated for extended periods of time following infection, and they also identify proteins phosphorylated in a p38-dependent manner in the context of viral infection. Identifying these substrates is significant since it will allow future investigation into the biological function of p38 in HCMV infection. Finally, several lines of evidence presented here suggest that HCMV has developed multiple mechanisms to ensure activation of a cellular kinase which is critical to initiation of viral DNA replication. Targeting these mechanisms may provide a means to inhibit HCMV-mediated p38 activation and viral permissiveness.

	MATERIALS AND METHODS

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Cell culture and viral passage. Human embryonic lung (HEL) fibroblasts were cultured in Eagle's minimal essential media (EMEM) (Gibco, BRL) supplemented with 10% fetal bovine serum (FBS) plus antibiotics. The human astrocytoma cell line U373MG was grown in Dulbecco's modified Eagle medium (DMEM) (Gibco, BRL) supplemented with 10% FBS plus antibiotics. Towne strain HCMV (passages 36 to 40) was propagated in HEL fibroblasts as previously described (22).

Expression vectors and cell lines. FLAG-pCDNA3-MKK3 dominant negative (MKK3^dn) and FLAG-pCDNA3-MKK6 dominant negative (MKK6^dn) expression vectors were kindly provided by Roger Davis and have been previously described (44). To obtain cell lines, U373 cells were seeded in 35-mm-diameter six-well plates at 10⁶ cells/well. The following day they were transfected with the indicated plasmid, using Fugene 6 as specified by the manufacturer (Boehringer GmbH, Mannheim, Germany). Forty-eight hours later, fresh DMEM containing 10% FBS, antibiotics, and G418 (800 µg/ml) was added to each well. The medium was changed every 5 days. After G418 selection, the expression of transfected protein in U373 was confirmed by Western blot analysis, immunoprecipitation, and immunofluorescence.

Antibodies and inhibitor drugs. All phosphospecific antibodies and the p38 nonphosphospecific antibody were purchased from New England Biolabs (NEB; Beverly, Mass.). FLAG antibody M2 was obtained from Kodak (Rochester, N.Y.). pRb antibody was from Santa Cruz Biotechnology (Santa Cruz, Calif.), and cyclin D and cyclin E antibodies were from Oncogene Science (Cambridge, Mass.). HSP27 antibody was from Sigma (St. Louis, Mo.). Anisomycin, rotenone, and 2-deoxyglucose were purchased from Sigma. The specific p38 inhibitor drug FHPI was purchased from Calbiochem (La Jolla, Calif.).

Western blot analysis. Fibroblasts were grown to confluence, serum starved for 48 h, and then infected with HCMV at a multiplicity of infection (MOI) of 2 to 5 PFU per cell as described elsewhere (19). In experiments which utilized FHPI, cells were pretreated with 10 µM FHPI for 1 h prior to infection. Following pretreatment, cells were infected and incubated in the presence of 10 µM FHPI as previously described (19). At the indicated times, cells were harvested in 2× Laemmli sodium dodecyl (SDS) sample buffer, boiled, and loaded onto SDS-polyacrylamide gels. Mock-infected samples were treated and harvested in the same manner as the infected samples, except that EMEM without virus was used during the infection. Proteins were separated by polyacrylamide gel electrophoresis (PAGE) and transferred overnight at 14 V to Immobilon-P transfer membranes (Millipore, Bedford, Mass.). Blots were blocked for 30 min in 10% (wt/vol) Carnation nonfat dry milk dissolved in phosphate-buffered saline (PBS) with 0.1% Tween 20 (PBS+T). Blots were then probed with primary antibody (1:1,000 dilution) for 2 h at room temperature or overnight at 4°C in PBS+T. Blots were washed three times with PBS+T. After washing, the blots were probed with secondary antibody (horseradish peroxidase-conjugated anti-mouse or anti-rabbit immunoglobulin G [Sigma or NEB, respectively]). Blots were washed in PBS+T and developed by enhanced chemiluminescence as specified by the manufacturer (NEB).

Immunoprecipitations and kinase assays. Glutathione S-transferase (GST)-c-Jun (amino acids [aa] 1 to 79) and GST-ATF-2 (full length) were purchased from NEB. p38 and JNK kinase assays were performed as previously described (43). Briefly, cells were pretreated and infected as described above. At the indicated times, cells were harvested in lysis buffer (150 mM NaCl, 20 mM Tris-HCl [pH 7.5], 1.0% Triton X-100, 0.5 mM EDTA, 50 mM NaF, 10% glycerol, 20 µg of leupeptin per ml, 20 µg of phenylmethylsulfonyl fluoride [PMSF] per ml, 1 mM sodium vanadate [Na₃VO₄]), rocked for 15 min at 4°C, and then vortexed for 15 seconds. Cell debris was pelleted by centrifugation, and the concentration of the supernatant was determined by the Bio-Rad protein assay. For the JNK kinase assay, 75 µg of supernatant was rocked for 4 h at 4°C with 1 µg of GST-c-Jun bound to GST beads (Biochem Pharmacia). For the p38 assay, p38 was immunoprecipitated overnight from 200 µg of supernatant in ELB⁺ (0.25 M NaCl, 0.1% NP-40, 0.05 M HEPES [pH 7.0], 1 mM PMSF, 0.5 mM EDTA, 0.05 mM dithiothreitol [DTT]) plus 0.1 mM Na₃VO₄ with a p38 polyclonal antibody and 15 µl of protein G-Sepharose beads (Biochem Pharmacia). After the appropriate incubation, the beads were washed three times in lysis buffer, and once in kinase buffer (20 mM HEPES [pH 7.5], 10 mM MgCl₂, 50 mM NaCl₂, 5 mM -glycerophosphate, 1.5 mM EGTA, 1 mM PMSF, 0.5 mM EDTA, 0.05 mM DTT, 0.1 mM Na₃VO₄). Kinase assays were then performed, using 5 µCi of [-³²P]ATP/reaction and, in the case of p38, addition of 1 µg of GST-ATF-2. After a 20-min incubation at 30°C, the beads were washed once and then denatured with 2× SDS sample buffer. After boiling, the samples were separated by SDS-PAGE on a 12% polyacrylamide gel, dried, and subjected to autoradiography. Bands were quantitated with a densitometer.

Heat treatment and ATP depletion. Confluent, serum-starved cells were washed once with EMEM which had been prewarmed to 45°C and then incubated in EMEM at 45°C for 20 min. ATP depletion was performed as previously described (35). Briefly, following infection or heat treatment, cells were washed twice with PBS which was preheated to 37°C. Cells were then incubated with PBS supplemented with 5 µM rotenone and 10 mM 2-deoxyglucose at 37°C for the indicated times. Following the appropriated incubation, cells were washed twice with PBS and harvested in 2× SDS lysis buffer.

In vivo analysis of HSP27 phosphorylation. Confluent, serum-starved fibroblasts were infected as described above. Three hours prior to harvesting, cells were washed twice with phosphate-free EMEM prewarmed to 37°C and then incubated with 0.4 mCi of [³²P]orthophosphate per ml in phosphate-free EMEM (ICN). Following a 3-h incubation, cells were harvested and protein concentration was determined by the Bio-Rad protein assay as described above. HSP27 was immunoprecipitated as described above and separated by SDS-PAGE on a 12% gel. HSP27 phosphorylation was then determined by autoradiography.

	RESULTS

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Kinetics of p38 activation following viral infection. Inhibition of p38 kinase activity by treatment of infected cells with FHPI significantly inhibits HCMV DNA replication and decreases viral titers (19). Determining the mechanism of HCMV-mediated p38 activation could identify new targets to inhibit this activation and, therefore, viral permissiveness. Since little is known about p38 activation following HCMV infection, it was first necessary to determine the kinetics of p38 activation during HCMV infection. With this information, we could focus our investigation of the mechanism of HCMV-mediated p38 activation on those times which correlated with increased levels of p38 activity.

View larger version (28K): [in this window] [in a new window]   FIG. 1.   Activation of p38 following HCMV infection. HEL fibroblasts were infected with sucrose gradient-purified virus at an MOI of 2 to 5 PFU. Cells were then harvested in 2× SDS sample buffer at the indicated time points, and Western blot analysis using a p38 phosphospecific antibody was performed. (A) Time course examining the effect of HCMV infection on p38 activation at times corresponding to the first tier of activation of host cell transcription factors. (B) (Top) Western blot demonstrating that p38 is phosphorylated on activating residues beginning at 10 hpi; (bottom) Western blot using p38 antibody, which recognizes all forms of p38, demonstrating that the overall levels of p38 do not fluctuate. (C) Viral protein synthesis is necessary for HCMV-mediated p38 activation. Fibroblasts were infected with unpurified virus stock at an MOI of 2 to 5 PFU. Where indicated, virus was inactivated by UV irradiation prior to infection. Blots were also probed for IE1-72 and IE2-86 gene expression to demonstrate inactivation of virus by UV irradiation (UV-VIRUS). p.i., postinfection; (M), mock-infected cells; (I), infected cells; UV, UV-irradiated HEL fibroblasts [positive control]); Phos., phosphorylated. The results are representative of five experiments.

View larger version (12K): [in this window] [in a new window]   FIG. 2.   HCMV-induced p38 phosphorylation correlates with increases in p38 kinase activity. Fibroblasts were treated and infected as for Fig. 1. At the indicated time points, cells were harvested in lysis buffer, and p38 kinase activity was determined by using GST-ATF-2 (aa 1 to 254) as a substrate. Autoradiography was analyzed with a densitometer, and the quantitation is shown. (M), mock infected. The data are representative of two separate experiments.

HCMV infection results in hyperphosphorylation of pRb and phosphorylation of HSP27 in a p38-dependent manner. The results in Fig. 1 and 2 suggest that p38 has a role in HCMV infection throughout the course of infection. However, no one has yet examined if the extended activation of p38 correlates with activation of any p38 substrates in the context of viral infection. For this reason, we sought to identify substrates which are phosphorylated in a p38-dependent manner following viral infection. Following activation by certain stimuli, p38 phosphorylates the pocket protein pRb and HSP27 (13, 47, 50). Furthermore, under specific conditions, increased p38 kinase activity can regulate expression of the cell cycle-regulating protein cyclin D (25).

View larger version (39K): [in this window] [in a new window]   FIG. 3.   Inhibition of p38 kinase activity inhibits HCMV-mediated pRb hyperphosphorylation but does not effect HCMV mediated changes in cyclin E or cyclin D protein levels. Fibroblasts were grown to confluence and then serum starved for 48 h. Cells were pretreated for 1 h with 10 µM FHPI prior to infection with HCMV. HCMV infection was done in the presence of FHPI, and extracts were harvested at the indicated times. Western blot analysis was performed with antibody for pRb (A), cyclin D (B), and cyclin E (C). M, mock infected; Phos., phosphorylated. Each experiment was performed at least three times, and representative blots are shown.

View larger version (20K): [in this window] [in a new window]   FIG. 4.   p38 kinase activity is necessary for phosphorylation of HSP27 following viral infection. Confluent, serum-starved fibroblasts were infected and then labeled with 0.4 mCi of [32P]orthophosphate per ml in the presence or absence of FHPI 3 h prior to being harvested. At the indicated times, cell extracts were harvested, and HSP27 was immunoprecipitated from 500 µg of whole-cell extract per sample, separated by SDS-PAGE on a 12% gel, and subjected to autoradiography. M, mock infected; Phos., phosphorylated.

HCMV infection does not activate MKK4 or JNK. The above results confirm that p38 has an active role in phosphorylating specific substrates for extended periods of time following infection. Since extended activation of p38 is unusual, we were interested in how HCMV was able to maintain p38 activation. This would further our understanding of the interaction between HCMV and the host cell, with the long-term view of identifying novel targets to inhibit HCMV-mediated p38 activation and, subsequently, viral permissive infection.

View larger version (22K): [in this window] [in a new window]   FIG. 5.   HCMV infection does not activate MKK4 or JNK. (A) Western blot analysis of infected whole-cell extract with an antibody which recognizes only active MKK4. For the positive control, HEL fibroblasts were treated with anisomycin (10 µg/ml) for 20 min and harvested in the same manner as the HCMV-infected cells. (B) JNK is not activated following HCMV infection. Cells were infected with HCMV and harvested in JNK lysis buffer, and JNK kinase activity was determined with GST-c-Jun (aa 1 to 79) as a substrate. Following autoradiography, band intensity was determined with a densitometer, and the quantitated results are shown. UV-irradiated fibroblasts were used as a positive control. (M), mock infected; Phos., phosphorylated. Each panel shows representative data from at least three separate experiments.

MKK3 and MKK6 are activated at late times of HCMV infection. Next, we determined if viral infection activated MKK3 and MKK6, the remaining two cellular kinases which are known to phosphorylate p38 (10). Interestingly, while we observed a strong increase in MKK3/6 phosphorylation at 48 and 72 hpi (Fig. 6A, lanes 5 and 6), only a very slight increase in phosphorylation was observed at 12 hpi (lanes 2 and 3). Again, Western blot analysis for -actin demonstrated that equal amounts of protein were loaded in each lane (Fig. 6B). Thus, increased MKK3/6 activity accounts for p38 activation at later times of infection (48 to 72 hpi). Since HCMV infection results in the activation of p38 as early as 8 hpi, we postulated that there must exist another mechanism to account for the strong activation of p38 at the earlier time points.

View larger version (22K): [in this window] [in a new window]   FIG. 6.   HCMV infection strongly activates MKK3/6 at later times of infection. (A) Fibroblast extract used in Fig. 5 were probed for MKK3/6 activation using an MKK3/6 phosphospecific antibody. Band intensity was quantitated with a densitometer. (B) Western blot analysis for -actin demonstrates equal protein loading in all samples. (M), mock infected; Phos., phosphorylated. The data shown are representative of results from four separate experiments.

Basal kinase activity of MKK3/6 is needed for p38 phosphorylation at early times of infection. In response to heat shock, JNK is activated through inhibition of dephosphorylation rather than increased activity of upstream kinases (35). This activation requires basal upstream kinase activity (in the case of JNK, basal MKK4 activity is required) and inhibition of dephosphorylation of activated JNK. We were interested in determining whether HCMV activated p38 in a similar mechanism, since we were unable to detect an increase in MKK activity at early times postinfection. To test this theory, it was first necessary to demonstrate that basal activity of an MKK is necessary for p38 activation following HCMV infection. Since there are no drugs available to inhibit MKK3/6 kinase activity, we made cell lines which expressed a nonphosphorylated MKK6 or MKK3 (MKK3^dn and MKK6^dn). These are dominant negative proteins which have their phosphorylation sites mutated, and when overexpressed, they prevent the wild-type protein from becoming phosphorylated (44).

View larger version (39K): [in this window] [in a new window]   FIG. 7.   Basal levels of MKK3/6 kinase activity are necessary and sufficient for HCMV-mediated p38 activation. U373 cells were transfected with an expression vector for either MKK3dn or MKK6dn. (A) After selection in medium containing G418 (800 µg/ml), Western blot analysis using a FLAG monoclonal antibody was performed to verify protein expression. (B) Cells were pretreated, infected, and harvested as for Fig. 1. Western blot analysis was performed with polyclonal phosphospecific (to demonstrate p38 activation) or nonphosphospecific (to demonstrate that overall levels of p38 were equal) p38 antibodies. Phos., phosphorylated. The data are representative of results from three separate experiments.

HCMV inhibits dephosphorylation of p38 at early but not late times of infection. The results in Fig. 7 suggest that p38 is activated by the low levels of MKK6 basal kinase activity that is present in unstimulated cells. For this basal kinase activity to increase the steady-state level of phosphorylated p38, dephosphorylation of active p38 needs to be inhibited. This would allow a buildup of activated p38. Therefore, the next step was to determine the effect of HCMV infection on dephosphorylation of active p38. Traditional pulse-chase assays are not sensitive enough for examining the inhibition of dephosphorylation under these experimental conditions (35). Therefore, to assess the effect of HCMV infection on dephosphorylation of p38, it was necessary to inhibit the ability of upstream kinases (such as MKK3 and MKK6) to phosphorylate p38 and then monitor the rate of decline in the levels of phosphorylated p38. To accomplish this task, we used the technique of ATP depletion, which was successfully used by Meriin et al. to examine the rate of dephosphorylation of p38 and JNK following various stress treatments (35). In this experiment, cells are treated with the indicated stress. ATP is then depleted from the cells, which inhibits upstream kinase activity. This prevents the cell from further phosphorylating p38, and therefore the rate of dephosphorylation is the sole determinant of the level of phosphorylated p38. Following ATP depletion, time points are taken, and the amount of phosphorylated protein is determined by Western blot analysis with phosphospecific antibodies. Meriine et al. also demonstrated that heat shock-mediated p38 activation was not mediated through inhibition of dephosphorylation (35). Therefore, we used heat shock treatment as our control for the rate of dephosphorylation.

View larger version (52K): [in this window] [in a new window]   FIG. 8.   HCMV infection inhibits dephosphorylation of active p38 at early, but not late, times of infection. (A) U373 cells were infected for 12 h or subjected to heat shock (45°C for 20 min). ATP was depleted by the addition of rotenone and 2-deoxyglucose, and cells were harvested at the indicated times after depletion. The amount of phosphorylated p38 was determined by Western blot analysis. (B) Cells were infected for 48 h and then subjected to ATP depletion and Western blot analysis as for panel A. Time 0, start of ATP depletion; M, mock-infected cells; Phos., phosphorylated.

View larger version (20K): [in this window] [in a new window]   FIG. 9.   Model for HCMV-mediated p38 activation. (A) At early times of infection, when no increase in MKK3/6 or MKK4 kinase activity is observed, the virus prevents phosphatases from recognizing activated p38. As basal MKK3/6 kinase activity continues to phosphorylate p38, phosphatases are unable to dephosphorylate p38, and so the amount of activated p38 accumulates. (B) At later times of infection, MKK3/6 kinase activity is increased, resulting in p38 activation.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

In vivo, primary targets for HCMV lytic infection are terminally differentiated cells in which many macromolecules and cellular pathways, including kinase pathways such as p38, which HCMV needs to undergo a lytic infection, are present at low levels or are inactive (53). Several recent studies have shown that HCMV infection elicits a mitogenic-like response which results in activation of these critical cellular components (see the introduction). However, little is known about the mechanism by which HCMV induces this activation. In this report, we have examined the kinetics and mechanisms of p38 activation following HCMV infection. In fibroblasts, viral protein expression is necessary to obtain p38 activation, which is first observed at 8 to 10 hpi (Fig. 1 and 2). This is somewhat surprising, since our laboratory recently reported that HCMV infection of monocytes results in a dramatic up regulation of p38 kinase activity by 15 min postinfection, independent of viral protein expression (55). One explanation for these differences is that HCMV may utilize different receptors to bind fibroblasts and monocytes. Alternatively, HCMV infection may regulate p38 activation differently in permissive cell types (such as fibroblasts) compared to nonpermissive cell types (such as monocytes). Currently, we are trying to address this issue by first identifying HCMV receptors on monocytes and fibroblasts. In addition, we are comparing activation of p38 following HCMV infection of other permissive and nonpermissive cell types.

We were also unable to detect p38 activation following infection of unpurified viral stock in which HCMV had been inactivated by UV irradiation prior to infection (Fig. 1C). This suggests that secreted cytokines released by infected cells into the media are not responsible for p38 activation in this case. Based on these results, we believe it most likely that viral proteins themselves are responsible for p38 activation throughout the course of infection.

We then provide evidence that HCMV infection induces hyperphosphorylation of pRb and HSP27 in a p38-dependent manner. These results not only demonstrate that p38 is functionally active throughout infection but also provide insight into how p38 may mediate its biological effects on viral DNA replication. pRb regulates expression of many cellular proteins associated with DNA synthesis, and many of these proteins are thought to be necessary for viral DNA replication (51). At early stages of the cell cycle, pRb is hypophosphorylated, and in this form it acts as an inhibitor of gene expression, in most instances by directly complexing with transcription factors such as E2F and suppressing their transactivation function (51). Upon exposure to a proliferative signal, the cell induces hyperphosphorylation of pRb. Once hyperphosphorylated, pRb loses its ability to suppress transactivation function, which results in transcription of the genes necessary for progression into S phase and subsequent cellular DNA synthesis.

The classical binding partner for pRb is the E2F family of transcription factors, and hyperphosphorylation of pRb is necessary for relieving pRb-mediated suppression of E2F, which results in cell cycle progression past the G₁/S phase transition point (reviewed in reference 39). However, the HCMV IE2-86 protein binds to pRb and alleviates pRb-mediated suppression of E2F transactivation function (15). Furthermore, IE2-86 has a high affinity for hypophosphorylated pRb, suggesting that HCMV can induce E2F transactivation function in the absence of pRb hyperphosphorylation (15). Therefore, while FHPI inhibits the pRb hyperphosphorylation observed following HCMV infection, it is likely that it does not inhibit pRb-mediated suppression of E2F transactivation function in the context of viral infection. Indeed, our preliminary results indicate this is the case (data not shown). However, pRb also regulates the activity of several other proteins in a phosphorylation-dependent manner. As with E2F, these proteins, which include histone deacetylases and RNA polymerase III, regulate expression of a variety of cellular genes involved in cell cycle progression and likely have important functions in viral infection (4, 24, 25, 32, 33). Currently, we are investigating the role of these other proteins in viral infection.

HSP27 appears to have a critical role in protecting cells from apoptosis following certain stresses (reviewed in references 8 and 14). One mechanism by which it does this is by acting as a molecular chaperone. It binds to proteins and can either prevent their incorrect folding or association with inhibitory proteins and/or ensure their translocation between subcellular compartments (14). In the context of viral infection, p38-mediated activation of HSP27 could have two functions. It might serve to inhibit apoptosis following external stresses. This would ensure that the virus is able to complete its life cycle before the cell dies. Alternatively, infection itself is a type of stress and may cause inactivation of proteins which are necessary for viral permissiveness, due to either misfolding or incorrect compartmental localization. Therefore, HCMV may activate HSP27 to ensure that these proteins are active and able to perform their function as it relates to HCMV-permissive infection. As with the pRb hyperphosphorylation, we are currently addressing these possibilities.

The next series of experiments characterized the mechanism of HCMV-mediated p38 activation. Using ATP depletion, we provided evidence that at early times of infection, HCMV inhibited dephosphorylation of activated p38, allowing an increase in the steady-state levels of phosphorylated p38 in the presence of only basal MKK6 kinase activity. We also consistently detected an increase in the amount of active p38 immediately following addition of ATP-depleting reagents but before ATP had been depleted from the cells (Fig. 8, compare lanes 2 and 3). This increase was not due to the process of ATP depletion, as no increase in the amount of phosphorylated p38 was detected following addition of ATP-depleting reagents to either heat-shocked cells or cells infected with HCMV for 48 h. Little is known about the mechanism which mediates activation of p38 or JNK by inhibition of dephosphorylation, and therefore it is difficult to speculate on why this increase occurs. It may be due to the efficient use of residual ATP by HCMV-infected cells before the ATP depletion is complete. Alternatively, it may be a characteristic of stimuli which activates p38 or JNK by inhibition of dephosphorylation. We are currently addressing these questions experimentally.

While inhibition of dephosphorylation is an uncommon mechanism for inducing MAPK activation, a similar mechanism was recently found for heat shock-mediated activation of JNK (35). In that study, basal MKK4 kinase activity coupled with inhibition of JNK dephosphorylation was sufficient to induce JNK activation. Two other reports have been published, both examining activation of ERK1/2 by inhibition of dephosphorylation. The first report described inhibition of protein phosphatase PP2A activity by SV40 small T antigen (41). This inhibition allowed accumulation of phosphorylated MKK1/2 and ERK in the absence of increased MKKK activity (54). A second report demonstrated that activation of ERK1/2 following HCMV infection of prestimulated fibroblasts may be at least partially due to inhibition of ERK1/2 dephosphorylation (46).

It may be fortuitous for HCMV to use an unusual mechanism to activate p38. For instance, increased MKK6 kinase activity has been implicated in some types of apoptosis (18). By not increasing MKK6 kinase activity, HCMV may avoid this effect, which would be detrimental to a successful viral life cycle. Also, many stimuli that activate p38 by activation of upstream kinases also activate JNK. Recently, it has been suggested that the antiviral property of tumor necrosis factor alpha may be due to its ability to activate JNK (1). By activating p38 through inhibition of dephosphorylation rather than increasing kinase activity of MKKs, perhaps HCMV ensures that JNK is not activated. This theory is supported by our findings that neither MKK4 nor JNK is activated following infection (Fig. 5).

HCMV could inhibit dephosphorylation of activated p38 by two mechanisms. It could directly bind to and inactivate one or more p38-specific phosphatases. This would be analogous to SV40 small T antigen, which binds to a subunit of the PP2A complex, preventing the formation of an active PP2A (41, 54). Alternatively, it could somehow prevent active phosphatases from recognizing p38, perhaps by binding p38 in such a way that it is masked from the phosphatases. We have found that p38 phosphatases are strongly activated following infection (data not shown), which correlates with previously published data showing that PP2A and PP1, both of which can dephosphorylate p38, are activated following HCMV infection (36). These findings suggest that p38 is likely activated by the latter mechanism (activated phosphatases cannot recognize phosphorylated p38) following HCMV infection.

Currently, we are addressing the question of which viral proteins are responsible for the inhibition of dephosphorylation. One problem with identifying these candidates is that it is difficult to determine the exact time at which the infected cell begins accumulating phosphorylated p38. The earliest we have been able to detect p38 phosphorylation is 6 to 8 hpi. However, since activation depends on basal kinase activity, it may be quite some time between the commencement of inhibition of p38 dephosphorylation and the detection of p38 activation. We have looked for the ability of the two major viral IE proteins (IE1-72 and IE2-86) to activate p38 but have been unable to induce p38 activation by overexpression of either IE2-86 or IE1-72 (data not shown). Aside from the possibility that these proteins may not be involved in p38 activation, two alternate explanations for why we have been unable to detect p38 activation by specific viral proteins are that posttranslational modification of IE proteins, such as phosphorylation, and/or expression of multiple viral genes is necessary to obtain p38 activation. Our search has been further complicated by the fact that different mechanisms are utilized to activate p38 at early and late times of infection. Currently, we are developing better techniques to identify the viral proteins necessary for p38 activation.

We went on to show that at later times of infection, HCMV no longer inhibited dephosphorylation of activated p38 (Fig. 8B). However, MKK3/6 activity was increased at these later times of infection (Fig. 6), and this increased activity was sufficient to increase the steady-state level of activated p38. This finding indicates HCMV utilized a different mechanism to activate p38 at later times of infection. These results may also explain why a decrease in p38 phosphorylation was often observed at 24 hpi. The ability of HCMV to inhibit dephosphorylation of p38 may be lost before MKK3/6 is activated. At that time of infection, no mechanism to activate p38 would be present, and the levels of phosphorylated p38 would drop.

This characterization of HCMV-mediated p38 activation provides important new insight into the complex interaction between HCMV and the infected host cell. We have demonstrated that p38 is activated during viral infection. We have also identified two p38 substrates which are phosphorylated in a p38-dependent manner in the context of a viral infection, which will be helpful in future studies of the biology of p38 function in the context of viral infection. Finally, this study provides strong evidence that HCMV activates p38 by two different mechanisms, including the very unusual mechanism of inhibition of dephosphorylation. Since it is unusual, it may provide a future target for preventing p38 activation and, subsequently, HCMV infection.