INTRODUCTION

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Varicella-zoster virus (VZV), one of eight herpesviruses known to infect humans, is classified along with herpes simplex virus types 1 and 2 (HSV-1 and 2) as an alphaherpesvirus based on its growth characteristics and ability to become latent in the nervous system of the host (28). Unlike the other alphaherpesviruses, however, VZV induces syncytia and is highly cell associated when grown in cultured cells, indicating that its cellular egress pathway differs from that of the other alphaherpesviruses.

Herpesvirus virions acquire their initial envelope upon budding through the inner nuclear membrane into the perinuclear space (8, 11, 22, 30). Following envelopment at the inner nuclear membrane, the route taken by virions as they transit through and ultimately exit the cell is less certain and may vary between different herpesviruses (5, 20, 34). Regardless of the site at which the final envelope is acquired, however, the importance of proper glycoprotein transport and processing during egress has been demonstrated experimentally. When cells defective for the intracellular transport or glycosylation of viral glycoproteins are infected with HSV-1, human cytomegalovirus (HCMV), or pseudorabies virus, the resultant virions fail to egress normally and instead accumulate in cytoplasmic vacuoles (2, 12, 35).

All known herpesviruses encode a glycoprotein B (gB) homolog. VZV gB contains 868 amino acids (aa) and is a type I membrane protein consisting of a large ectodomain, a hydrophobic transmembrane region, and a cytoplasmic domain. The cytoplasmic domain of VZV gB is predicted to contain 125 aa, making it by far the longest cytoplasmic domain of any VZV membrane proteins (6). In the alphaherpesviruses, gB is present in the virion envelope, where it is thought to play a role in fusion of the virion and plasma membranes following viral attachment (3, 26, 31). gB also plays an important role in viral egress, as evidenced by the existence of numerous HSV-1 gB mutants that promote syncytium formation in cultured cells (1, 26). Many of the syncytium-generating mutations are in the cytoplasmic domain of HSV-1 gB, suggesting that this domain may be particularly important for viral egress. In addition, Epstein-Barr virus (EBV) gB, while unlikely to be essential for viral entry because little, if any, is present in the virion envelope (10), is nonetheless required for the production of infectious virions, suggesting a function primarily during viral egress (16, 17).

The cytoplasmic domains of many membrane proteins, including VZV gE and gI, contain specific signal sequences that mediate their post-Golgi intracellular transport (14, 16, 24, 25, 32, 37). In addition, the efficient endoplasmic reticulum (ER)-to-Golgi transport of HSV-1 gB requires an intact cytoplasmic domain. HSV-1 containing large C-terminal deletions in its cytoplasmic domain (66 to 109 aa) was transported to the Golgi more slowly than native gB (27), and linker insertion mutations at various places in the cytoplasmic domain of HSV-1 gB also slowed ER-to-Golgi transport (3). While several specific sequence motifs that mediate the post-Golgi transport of membrane proteins have been identified (14, 32), ER-to-Golgi transport signal sequences are less well characterized. Recently, a signal sequence that mediates the transport of vesicular stomatitis virus G protein (VSV-G) from the ER to the Golgi was identified within the cytoplasmic domain of this type I transmembrane protein (23). This sequence, YTDIE, contains a YXX motif and two acidic amino acids (in the pattern DXE), which are essential for its function. Several other transmembrane proteins have been identified that contain YXX motifs followed at variable distances (2 to 6 aa) by acidic amino acids in the pattern (D/E)X(D/E). However, it is not known whether these motifs function during protein transport.

The cytoplasmic domain of VZV gB contains two YXX motifs, one (underlined) followed by a single acidic residue (YSRVSRE, aa 857 to 863) and one (underlined) followed by two acidic residues in the pattern EXXE (YMTLVSAAERQE, aa 818 to 829). To determine whether these or other regions of the VZV gB cytoplasmic domain mediate the ER-to-Golgi transport of gB, we introduced deletions and specific point mutations into the cytoplasmic domain of gB, expressed the mutated proteins in mammalian cells, and assessed their transport to the Golgi. The C-terminal 17 aa of VZV gB (residues 852 to 868) and the 9-aa region between residues 818 and 826 were found to be critical for the transport of VZV gB from the ER to the Golgi.

	MATERIALS AND METHODS

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Cell culture and virus propagation. MeWo, an immortalized human melanoma cell line, and HEp-2, a human epithelial cell line, were grown in Eagle's minimum essential medium (EMEM) containing 10% fetal bovine serum (FBS; Bio-Whittaker) and 2 mM L-glutamine (Quality Biological). Recombinant vaccinia virus vTF7-3 (21) was obtained from the American Type Culture Collection, and viral stocks were prepared and titered in BSC-40 African green monkey kidney cells that were also grown in EMEM containing 10% FBS and L-glutamine.

Immune reagents and intracellular markers. Anti-VZV gB monoclonal antibodies (MAbs) were purchased from Biodesign International (catalog no. C05102M). Tetramethylrhodamine isothiocyanate (TRITC)-conjugated wheat germ agglutinin (WGA) was purchased from EY Laboratories. Rabbit anticalnexin polyclonal antibodies were purchased from StressGen. Goat anti-mouse immunoglobulin G (IgG) conjugated with fluorescein isothiocyanate (FITC) and TRITC-conjugated goat anti-rabbit IgG were purchased from Sigma.

Plasmid construction and site-directed mutagenesis of VZV gB. An 8,131-bp PmeI/SpeI fragment of VZV strain Oka (consensus nucleotides 53875 to 62007 [12]) containing the VZV gB coding sequence (nucleotides 57008 to 59611) was cloned into pNEB193 (New England Biolabs) in which the SmaI site had been replaced with a SpeI linker. The 8,148-bp fragment resulting from HindIII/SpeI digestion of this plasmid (8,131-bp VZV DNA fragment and 17 bp from the pNEB polylinker) was cloned into pBluescript SK+ (Stratagene) at the corresponding restriction sites such that the gB coding sequence was downstream of the T7 promoter to yield pBS-8131. A 2,329-bp DNA fragment between the T7 promoter and the gB start codon was eliminated by digesting pBS-8131 with StuI and HindIII followed by recircularization to yield pBS-5802.

View larger version (44K): [in this window] [in a new window]   FIG. 1.   VZV gB cytoplasmic domain mutations. The 125-aa residues (from aa 744 to 868) that make up the cytoplasmic domain of VZV gB are shown. The lines bisecting the cytoplasmic domain are located immediately after the terminal amino acids of the truncation mutants used in this study. These mutants are named according to the number of deleted C-terminal amino acids. Substitution mutations are indicated by lines and brackets, and the amino acids replacing the native residue(s) are noted. The regions in red (aa 818 to 826), blue (aa 833 to 851), and green (aa 852 to 860) denote separate internal deletion mutations.

In vitro expression of VZV gB. VZV gB was expressed in vitro by the method of Fuerst et al. (7), modified as follows. DNA for transfection was column purified (Qiagen) and transfected using Lipofectin (Gibco-BRL). Cells at 75 to 90% confluence were infected with recombinant vaccinia virus vTF7-3 at a multiplicity of infection of 10 and then transfected with 7 µg of DNA and 12 µl of Lipofectin in 10-cm² wells or 3.5 µl of DNA and 6 µl of Lipofectin in 1.2-cm² wells. Cells were incubated at 37°C and 5% CO₂ for 16 h prior to metabolic labeling or fluorescent staining.

Radiolabeling and immunoprecipitation of proteins. All metabolic labeling was performed at 37°C in 5% CO₂. For steady-state labeling of in vitro-expressed gB, transfected cells were incubated for 1 h in EMEM lacking cysteine and methionine (Cys, Met EMEM) (starvation period) and then incubated in Cys, Met EMEM containing 125 µCi of Tran³⁵S-label (ICN) per ml for 4 h. To pulse-label in vitro-expressed gB, transfected cells were incubated for 1 h in Cys, Met EMEM containing 125 µCi of Tran³⁵S-label without prior starvation. Following the 1-h labeling period, cells were washed and incubated for 4 h in chase medium (EMEM containing 10% FBS, 24 µg of cysteine per ml, and 15 µg of methionine per ml). Labeled cells were washed extensively in phosphate-buffered saline (PBS) at 4°C and lysed in PBS containing 1% Triton X-100, 0.5% deoxycholate, and 0.1% sodium dodecyl sulfate (SDS). VZV gB was immunoprecipitated by incubating the cell lysates with anti-VZV gB MAbs overnight at 4°C followed by incubation with Staphylococcus protein G (Pharmacia Biotech) for 1 h at 4°C. After washing, precipitated proteins were eluted in sample buffer containing 2% SDS (Bio-Rad) and resolved by SDS-polyacrylamide gel electrophoresis (PAGE) on 8% gels. For reducing gels, 40 mM dithiothreitol was added to the sample buffer prior to elution. The gels were dried, and the labeled immunoprecipitated proteins were visualized by autoradiography.

Carbohydrate analysis. Radiolabeled proteins were immunoprecipitated as described above and then heated at 98°C for 3 min in 10 µl of 0.2% SDS-50 mM Tris-HCl (pH 6.8). After cooling to room temperature, 10 µl of 0.15 M sodium citrate (pH 5.3) and 1 µl (0.005 U) of endoglycosidase H (endo H; Boehringer Mannheim) were added. The reaction mixtures were incubated overnight at 37°C. Sample buffer was added to the endo H-treated proteins, and they were resolved by SDS-PAGE on 8% gels.

Quantitation of immunoprecipitated proteins. Following autoradiography of immunoprecipitated gB, the proportion of endo H-resistant gB (high-molecular-weight [high-MW] form) relative to endo H-sensitive gB (low-MW form) was quantitated for each sample by measuring the intensities of the corresponding bands using a Molecular Dynamics densitometer. All reported values represent the averages of at least four independent experiments.

Immunofluorescence and confocal microscopy. VZV gB expressed in transfected cells was detected by indirect immunofluorescence assays using anti-VZV gB MAbs. At 16 h after transfection and infection with vTF7-3, cells on glass coverslips were fixed for 1 h at 4°C in 2% paraformaldehyde. To permeabilize cellular membranes in experiments designed to visualize intracellular structures, 0.1% Triton X-100 was added to the fixative. Fixed cells were blocked by incubation with PBS containing 1% goat serum (Sigma) for 1 h at room temperature, then incubated overnight at 4°C with the primary antibodies (mouse anti-VZV gB MAbs diluted 1:500 in PBS and/or rabbit anticalnexin polyclonal antibody diluted 1:200 in PBS), washed in PBS, and incubated for 1 h at room temperature with the appropriate secondary antibodies (goat anti-mouse IgG FITC diluted 1:1,000 in PBS and/or goat anti-rabbit IgG-TRITC diluted 1:100 in PBS). In colocalization experiments requiring Golgi visualization, WGA-TRITC diluted to 5 µg/ml in PBS was incubated with the fixed cells for 20 min concurrent with the secondary antibody incubation. After washing in PBS, stained cells were examined for immunofluorescence with a Bio-Rad MRC1024 scanning confocal microscope.

	RESULTS

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VZV gB at steady state concentrates in the Golgi, the nuclear membrane, and the plasma membrane of both infected and transfected cells. We studied the intracellular localization of VZV gB in mammalian cells by indirect immunofluorescence. VZV gB expressed following either infection or transfection of MeWo and HEp-2 cells was detected using anti-VZV gB MAbs and then visualized with FITC-conjugated secondary antibodies. The Golgi apparatus was identified in the same cells by costaining with TRITC-conjugated WGA, a lectin that binds to complex oligosaccharides that are present in high abundance in the Golgi (4, 8). The nuclear and plasma membranes were also stained by TRITC-WGA. The cells were examined by laser scanning confocal microscopy. Figure 2 (top row) shows that native gB expressed early following VZV infection (at 24 h after inoculation) concentrated in the Golgi based on its colocalization with WGA. It also appeared in the nuclear membrane although some of this staining may represent gB in the ER, as these structures are contiguous. We could not determine from these studies whether gB was present in only the inner or outer nuclear membrane or in both. Later in VZV infection, the internal structures of infected cells became disrupted, making the identification of specific organelles difficult (data not shown). Native gB transiently expressed in cultured cells following transfection localized to the Golgi, the nuclear membrane, and the plasma membrane similarly to gB expressed during infection with VZV (Fig. 2, second row). This demonstrates that VZV gB localizes to the Golgi as well as to the nuclear and plasma membranes independently of interactions with other viral proteins.

View larger version (56K): [in this window] [in a new window]   FIG. 2.   Microscopic localization of VZV gB. Native gB or gB containing cytoplasmic domain C-terminal truncations was expressed in mammalian cells by transfection. Cells were also infected with native VZV as positive control. MeWo cells are shown; similar results were obtained with HEp-2 cells. Transfected or infected cells were incubated with both anti-VZV gB MAbs and WGA (a lectin that concentrates in the Golgi). The intracellular localization of gB and WGA was analyzed by laser-scanning confocal microscopy. Arrows mark the location of the Golgi in selected cells.

The cytoplasmic tail of VZV gB is required for Golgi localization. To determine whether the cytoplasmic domain of gB is required for its Golgi localization, we expressed gB containing mutations in its cytoplasmic domain in both MeWo and HEp-2 cells as described above. VZV gB was detected in these cells using anti-VZV gB MAbs and visualized by confocal microscopy. TRITC-conjugated WGA was again used as a colocalization marker for the Golgi. VZV gB lacking 17 or 67 aa from its C terminus (Fig. 1) failed to accumulate within the Golgi (Fig. 2). However, expression of gB in the nuclear envelope was seemingly unaffected by these C-terminal truncations (Fig. 2), and even removal of the entire 125-aa cytoplasmic domain of gB had no apparent effect on its nuclear membrane expression (data not shown). Several other gB cytoplasmic domain amino acid substitutions eliminating single or multiple residues did not alter the Golgi or nuclear envelope localization of gB (data not shown). These included mutations R751A, R756A, RKK-AAA (aa 833 to 835), and RNRR-ANAA (aa 851 to 855) (Fig. 1). To determine the intracellular location of the truncated forms of gB that failed to accumulate in the Golgi, cultured cells expressing gB lacking its C-terminal 17 or 67 aa were costained with anticalnexin antibodies as a marker for the ER. VZV gB containing both the 17- and 67-aa C-terminal truncations colocalized with calnexin, indicating that the mutated forms of gB are concentrated in the ER (Fig. 3). It should be noted that while the C-terminal truncations shown in Fig. 2 greatly diminish the transport of gB to the Golgi, the blockage is not absolute; therefore, some gB lacking the C-terminal 17 or 67 aa may be present in post-Golgi compartments as well. Together, these data indicate that the cytoplasmic domain of gB is required for the efficient transport of gB from the ER to the Golgi.

View larger version (59K): [in this window] [in a new window]   FIG. 3.   Microscopic localization of VZV gB. Native gB or gB containing cytoplasmic domain C-terminal truncations was expressed in mammalian cells by transfection. Transfected cells were coincubated with anti-VZV gB MAbs and anticalnexin antibodies (a marker of the ER). The intracellular localization of gB and calnexin was analyzed by laser-scanning confocal microscopy.

The C-terminal 17 aa of the VZV gB cytoplasmic domain are required for transport of gB from the ER to the Golgi. To define specific domains within the cytoplasmic domain of gB that mediate its ER-to-Golgi transport, we expressed gB or gB containing mutations in its cytoplasmic domain in cultured cells and evaluated their transport to the Golgi by assaying for the acquisition of two distinct Golgi-dependent posttranslational modifications: (i) proteolytic cleavage and (ii) conversion of N-linked oligosaccharides from high-mannose to complex forms.

View larger version (98K): [in this window] [in a new window]   FIG. 4.   Proteolytic cleavage of VZV gB cytoplasmic domain truncation mutants. Native gB (wt [wild type]) or gB containing cytoplasmic domain C-terminal truncation mutations was expressed in mammalian cells, immunoprecipitated, and resolved by SDS-PAGE under reducing conditions. Truncation mutants are designated by the number of amino acids removed from the C terminus. Mock-transfected cells () were included as a negative control. Native gB migrates as a 110-kDa species and as a doublet of about 60 kDa (resulting from its proteolytic cleavage in the Golgi). Molecular masses are given in kilodaltons.

View larger version (42K): [in this window] [in a new window]   FIG. 5.   ER-to-Golgi transport of VZV gB containing deletions in its cytoplasmic domain. Native gB (wt [wild type]) or gB containing cytoplasmic domain deletion mutations was expressed in mammalian cells, pulse-labeled, and then immunoprecipitated immediately (0-h chase) or after a 4-h chase. Treatment of immunoprecipitated gB with endo H is denoted by +. Mock-transfected cells (M) were processed identically as a negative control. The immunoprecipitated proteins were resolved by nonreducing SDS-PAGE on 8% gels.

View larger version (21K): [in this window] [in a new window]   FIG. 6.   Quantitation of ER-to-Golgi transport of VZV gB cytoplasmic domain deletion mutants. The C-terminal 54 aa of the VZV gB cytoplasmic domain (aa 815 to 868) are listed. Native (wild-type [wt]) gB contains this entire region, whereas the segments of this region retained by the various deletion mutants are represented by the black bars. The proportion of each mutant transported to the Golgi (as a percentage of native gB transport) was quantitated and is depicted by the stippled bars.

View larger version (25K): [in this window] [in a new window]   FIG. 7.   Quantitation of ER-to-Golgi transport of VZV gB mutants containing substitutions in the C-terminal 17 aa of its cytoplasmic domain. The C-terminal 17 aa of the VZV gB cytoplasmic domain (aa 852 to 868) are listed. Boldface letters represent substitution mutations within this region. The proportion of each mutant transported to the Golgi (as a percentage wild-type [wt] gB transport) was quantitated and is shown by the stippled bars.

The YXX-containing region from aa 818 to 826 within the cytoplasmic domain of gB is required for the transport of gB from the ER to the Golgi. The second YXX motif (underlined) in the cytoplasmic domain of VZV gB is followed by two acidic residues (boldface) in the pattern EXXE (YMTLVSAAERQE; aa 818 to 829) and thus closely resembles the predicted ER-to-Golgi transport signal sequences identified in other glycoproteins (23). We introduced mutations within and adjacent to this region (Fig. 1) to determine if it participates in the ER-to-Golgi transport of VZV gB. The efficiency of gB transport to the Golgi was again determined by quantitating the proportion gB acquiring complex oligosaccharides during a 4-h chase period following a 1-h labeling period. Several alanine substitution mutations within this region dramatically inhibited the transport of gB to the Golgi (Fig. 8; note that L821 was changed to glycine to avoid replacing one hydrophobic amino acid with another). The mutation Y818A resulted in only 50% native transport of gB to the Golgi, and mutations MT819-820AA, L821G, and E826A all resulted in less than 10% of the native level of gB transport from the ER to the Golgi (Fig. 8). By contrast, gB containing mutations proximal to this region (K817A) or carboxyl to aa 826 (RQ827-828AA, E829A, SK830-831AA, and RKK833-835AAA) was transported to the Golgi at levels similar to that of native VZV gB (Fig. 8). These data indicate that the region of the gB cytoplasmic domain between aa 818 and 826 is required for the efficient transport of gB from the ER to the Golgi, whereas the sequences immediately adjacent to this region do not contribute significantly to this process. To confirm this result, we deleted the entire region from aa 818 to 826 (YMTLVSAAE [Fig. 1]) from the gB cytoplasmic domain. This mutated form of gB, 818-826, was transported to the Golgi at less than 5% of the native level (Fig. 8). This is in sharp contrast to the nearby 19-aa deletion 833-851, which, as discussed previously, had little impact on the ER-to-Golgi transport of gB. These data indicate that the 9-aa region of the gB cytoplasmic domain between aa 818 and 826 is required for the efficient transport of gB from the ER to the Golgi. Moreover, several specific amino acids within this region are required for the ER-to-Golgi transport of gB, including those within the YMTL motif and the first of two acidic residues carboxyl to this motif, E826. Therefore, the region between aa 818 and 826, unlike the C-terminal 17-aa domain, participates in the ER-to-Golgi transport of gB through a sequence-dependent mechanism.

View larger version (34K): [in this window] [in a new window]   FIG. 8.   Quantitation of ER-to-Golgi transport of VZV gB mutants containing deletions or substitutions in the region between aa 816 and 837 of the cytoplasmic domain. Amino acid residues 816 to 837 of the VZV gB cytoplasmic domain are listed (Native gB). Amino acids 818 to 826 are underlined. Boldface letters represent substitution mutations within this region. The proportion of each mutant transported to the Golgi (as a percentage wild-type [wt] gB transport) was quantitated and is shown by the stippled bars.

	DISCUSSION

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We have shown that the cytoplasmic domain of VZV gB is critical for gB transport from the ER to the Golgi, and we have identified two specific regions within the gB cytoplasmic domain that are required for this process, the C-terminal 17 aa and an internal sequence consisting of aa 818 to 826. In addition, we have shown that VZV gB localization to the nuclear membrane cells occurs independently of its cytoplasmic domain.

Deletion of either the C-terminal 17 aa (aa 852 to 868) or aa 818 to 826 of VZV gB greatly diminishes the transport of gB from the ER to the Golgi. However, these regions apparently mediate the ER-to-Golgi transport of gB by different mechanisms. The C-terminal 17-aa domain functions independently of its amino acid sequence. Complete deletion of this region results in an almost complete loss of ER-to-Golgi transport; however, neither multiple substitution mutations nor internal deletions within this region had an impact on the ER-to-Golgi transport of gB. One possible explanation is that deletion of the C-terminal 17 aa of gB alters the conformation of its ER luminal domain, resulting in the retention of gB in the ER through its interaction with ER-resident chaperone proteins. It has, for example, been reported that mutations within the transmembrane domain of HCMV gB results in its ER retention by allowing the binding of specific chaperone proteins within the ER (36). This possibility, however, is rendered less likely for VZV gB by the observation that deletion of the adjacent 19 aa has little impact on the ER-to-Golgi transport of gB despite the expectation that this larger deletion would similarly alter the conformation of gB. Another possibility is that the C-terminal 17-aa domain functions through electrostatic rather than sequence-specific interactions with cellular factors. This model is attractive since the C-terminal 17-aa domain of gB contains five basic amino acids and a single acidic residue. Again, our experimental data fail to support this hypothesis, since gB in which three of these five basic residues are replaced by a neutral amino acid is transported to the Golgi as efficiently as native gB. We therefore consider it most likely that deletion of the C-terminal 17 aa affects the accessibility of an ER-to-Golgi translocation signal located elsewhere within the cytoplasmic domain itself such as the aa 818-826 domain.

In contrast to the C-terminal 17-aa domain, the VZV gB aa 818-826 domain functions in a sequence-dependent manner. This domain contains several individual amino acids that are required for the efficient ER-to-Golgi transport of gB, while numerous point mutations elsewhere in the cytoplasmic domain of gB, including those in which charged residues are eliminated, have no impact on transport. These data strongly suggest that the aa 818-826 domain functions as an independent translocation signal sequence that acts by a sequence-specific mechanism, perhaps through specific interactions with one or more cellular factors, rather than by nonspecific mechanisms based on protein conformation or electrostatic interactions.

Perhaps the most interesting sequence feature of the VZV gB aa 818-826 domain is its similarity to a known ER-to-Golgi transport signal, the YTDIE sequence found in the cytoplasmic domain of the VSV-G protein. Both the VZV gB aa 818-826 domain (YMTLVSAAE) and the VZV-G signal sequence contain YXX motifs and at least one acidic amino acid residue. Mutation of the acidic residue in the VZV gB aa 818-826 domain markedly impairs the transport of gB from the ER to the Golgi; however, mutation of a second downstream acidic residue, E829, has no impact on the transport of VZV gB. In contrast, mutation of either of the acidic residues in the VSV-G signal sequence greatly reduces its transport to the Golgi. Also, mutations within the YXX sequence itself in VZV gB aa 818 to 826 disrupt the ER-to-Golgi transport of gB, while only mutations in the acidic residues of the VSV-G signal sequence have a significant impact on the transport of VSV-G.

As expected, VZV gB that failed to be transported to the Golgi due to mutations in its cytoplasmic domain largely accumulated instead in the ER. However, gB containing large C-terminal truncations was transported to the nuclear membrane at apparently normal levels. These data indicate that the cytoplasmic domain of gB does not contain specific nuclear membrane transport signals. This is consistent with findings for HSV-1 gB, which requires only a portion of its transmembrane domain and none of its cytoplasmic domain for localization to the nuclear membrane (9). While specific nuclear membrane transport signals may be located in the luminal or transmembrane domains of VZV gB, it seems equally plausible that the transport of gB from the ER to the nuclear membrane occurs through passive diffusion of gB between these two contiguous structures.

Both the microscopic colocalization and posttranslational modification data presented here provide clear evidence that VZV gB is transported to the Golgi in the absence of other VZV-encoded proteins. It has, however, previously been reported that VZV gB cannot be detected in the trans-Golgi network of transfected cells (33). This seeming discrepancy may be due to the different cell types used in these studies. The studies which failed to show gB in the trans-Golgi network following transfection were done in Cos cells, whereas we used MeWo and HEp-2 cells. Cos cells, unlike MeWo and HEp-2 cells, do not support the growth of VZV, leading to the intriguing possibility that this deficiency may be due to the inability of Cos cells to correctly mediate the intracellular transport of VZV gB.

It is not known whether the cytoplasmic domain of other herpesvirus gBs contain specific ER-to-Golgi signal sequences analogous to those identified in VZV gB. A region closely related to the VZV gB aa 818-826 transport signal sequence exists in the cytoplasmic domain of HSV-1 gB. This 9-aa sequence, YMALVSAME, contains 7 aa that are identical to the VZV gB aa 818-826 domain and includes a YXX motif followed by an acidic residue. However, the VZV gB C-terminal 17-aa domain shown to be necessary for the transport of gB from the ER to the Golgi has little similarity to the C terminus of HSV-1 gB. Notably, HSV-1 gB contains five acidic residues among its C-terminal 7 aa, whereas VZV gB contains a single acidic residue among its C-terminal 7 aa.

The cytoplasmic domains of the beta- and gammaherpesviruses exhibit considerably less sequence homology to the VZV gB cytoplasmic domain. The cytoplasmic domain of HCMV gB contains the sequence YQMLLALAR (aa 845 to 853) at a site corresponding to the location the VZV gB aa 818-826 transport signal sequence. While this sequence contains a YXX motif, it has limited (3 of 9 aa) overall homology to the VZV gB aa 818-826 sequence and contains no acidic residues. The cytoplasmic domain of EBV gB contains no region with sequence homology to the VZV gB aa 818-826 domain. Moreover, the function of the EBV gB cytoplasmic domain contrasts sharply with that of the VZV gB cytoplasmic domain. While the VZV gB cytoplasmic domain facilitates the transport of gB from the ER to the Golgi, the EBV gB cytoplasmic domain inhibits the ER-to-Golgi transport of gB by means of an ER retention signal (16). Therefore, the cytoplasmic domains of gB homologs from members of different herpesvirus subfamilies may have in common their function as mediators of gB transport; however, their actual role in the intracellular localization of gB may vary dramatically between viruses from different subfamilies. The degree to which differences in gB localization influence the growth properties of the various herpesviruses remains to be defined.