INTRODUCTION

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The proper assembly of viral proteins and nucleic acids into a biologically active virion involves numerous and diverse macromolecular interactions. While structural proteins must correctly interact with other structural proteins, proper morphogenesis is equally dependent on interactions between structural and scaffolding proteins. Viral scaffolding proteins are transiently associated with morphogenetic intermediates but are not found in the mature viral particle (10, 12, 17, 22). These proteins promote the efficiency and fidelity of particle formation by ensuring proper interactions between viral proteins, promoting the nucleation of assembly, and aiding in the formation of a precisely sized capsid (13, 16, 19). In the Microviridae, assembly is dependent on two scaffolding proteins, an internal and an external species. The assembly pathway has been extensively characterized (8), and the atomic structures of both the X174 virion and a morphogenetic intermediate containing a full complement of scaffolding proteins have been solved (2, 3, 14, 15). Therefore, the results of genetic and biochemical analyses can be interpreted within a structural context.

The Microviridae assembly pathway is illustrated in Fig. 1. The first detectable morphogenetic intermediates are the 9S and 6S particles, respective pentamers of the viral coat and spike proteins. In a reaction mediated by the internal scaffolding (or B) protein, these intermediates associate, forming the 12S particle. Twelve 12S particles are then organized into the procapsid by 240 copies of the external scaffolding (or D) protein. The preinitiation complex, consisting of one copy of viral proteins A and C, replicative-form (RF) DNA, and the host cell Rep protein, associates with the procapsid, probably along a twofold axis of symmetry (5), forming the 50S complex. Single-stranded genomic DNA is then concurrently synthesized and packaged. The internal scaffolding protein is extruded during this reaction, yielding the infectious provirion, which may represent the end of the intracellular assembly pathway. The external scaffolding protein may dissociate upon cell lysis. External scaffolding proteins are not unique to the Microviridae. The coliphage P4 Sid protein and the triplex proteins of the Herpesviridae perform analogous functions (13, 23). Although the Microviridae morphogenetic pathway has been well characterized, the mechanism in which viral scaffolding proteins enable nascent intermediates to reach a biologically active form remains somewhat obscure. Here we report that the expression of closely related external scaffolding proteins inhibits morphogenesis. Using chimeric proteins, we have defined the existence of two inhibitory domains. These results have implications for the use of closely related proteins in the development of antiviral analogs specific to viral morphogenesis.

View larger version (56K): [in this window] [in a new window]   FIG. 1.   (A) The Microviridae morphogenetic pathway. In a reaction mediated by the internal scaffolding (or B) protein, 9S and 6S pentamers associate, forming the 12S particle. Twenty external scaffolding (or D) proteins add to this intermediate, which is then organized into the procapsid. The DNA binding (or J) protein enters the morphogenetic pathway during the packaging reaction, perhaps mediating the extrusion of the internal scaffold. Maturation is complete upon dissociation of the external scaffolding protein. (B) The four D proteins associated with each asymmetric unit (2). The subunits are designated D1 through D4; the amino and carboxyl termini are indicated where possible. The first  helices of D1 and D4 are depicted.

	MATERIALS AND METHODS

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Phage plating, media, burst experiments, stock preparation, generation of RF DNA, generation of radioactive lysates, and DNA isolation. The reagents, media, buffers, and protocols for burst experiments and single-stranded DNA isolation have been previously described (6), as has the generation and isolation of RF DNA (1). The protocols for producing radioactive lysates and subsequent sucrose gradient sedimentation analyses are the same as those previously described exception that [³⁵S]methionine and [³⁵S]cysteine were used to label viral proteins (5).

Bacterial strains. The Escherichia coli C strains C122 (sup^o), BAF5 (supE), and BAF30 recA have been previously described (1, 6). The C900 slyD host, used for the generation of radioactive lysates, was obtained from R. Young, Texas A&M University. The slyD mutation confers resistance to X174 E-protein-mediated lysis (18).

Phage mutants. The protocol used for oligonucleotide-mediated mutagenesis has been previously described (7). To generate the X174 NheI/PvuI strain, X174 am(D)Q22 DNA (7) was annealed to a mutagenic primer designed to concurrently eliminate the amber mutation and add the NheI restriction site. The mutant was selected for by the loss of the amber phenotype by transfection into C122 (sup^o). The NheI restriction site is located just after the region of the gene encoding the first  helix in the atomic structure of the D protein (2, 3). X174 NheI DNA was mutagenized to introduce the previously described (9) am(J)S7 mutation. The PvuI site was then introduced with a mutagenic primer designed to concurrently eliminate the amber mutation and add the PvuI restriction site. Again, selection against the amber phenotype was used. The PvuI site served as a marker for the identification of the chimeric genes (see below). The am(E)W4 mutation was then introduced as previously described (4). Similar methods were used to generate the 3 NheI, 3 NheI/am(E)W4, 3am(J)S7, and 3am(D)Q20 mutations. The PvuI and NheI restriction sites do not alter the amino acid sequences of the X174 or 3 D and J proteins.

Cloning of the X174 and 3 DJ, X and 3 J, and both chimeric D genes. Single-stranded DNA from both X174 NheI/PvuI am(E)W4 and 3 NheI/PvuI am(E)W4 mutants served as the template for PCR amplification of the D and J genes. The DJ fragments were cloned directly into the TOPO TA vector (Invitrogen) and then subcloned into the pSE420 expression vector (Invitrogen). The cloned genes are under lactose induction. The 3 J gene was cloned into pSE420 by digesting 3 RF DNA with HinPI and Bsp120I and digesting plasmid DNA with BstBI and NotI. All clones were verified by complementation experiments and RF digests. The clone of the X174 J gene has been previously described (9).

	RESULTS

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Cross-functional and cross-species inhibition analysis of the external scaffolding proteins. To determine whether the X174 and 3 external scaffolding proteins were capable of cross-complementation, nullD mutants were plated on cell lines harboring clones of the X174 or 3 D and J genes (Table 1). Despite 70% identity in amino acid sequence of the proteins, no cross-complementation was observed. Both null D mutants plated with efficiencies at least 1 order of magnitude below the reversion frequencies of the nullD mutations, as assayed on the C122 (sup^o) host. The failure to recover revertants suggested that the expression of the foreign proteins might confer cross-species inhibition. To determine if the expression of the foreign external scaffolding protein or DNA binding protein was affecting wild-type morphogenesis, wild-type X174 and 3 were plated on DJ plasmid-containing hosts and hosts harboring a clone of only the J genes. Expression of the J proteins failed to cross-inhibit either species of wild type, indicating that cross-inhibition was due to the overexpression of the scaffolding proteins.

View this table: [in this window] [in a new window]   TABLE 1.   EOPs of wild-type, nullD, and chiDr strains of X174 and 3 in cells expressing foreign and chimeric external scaffolding proteinsa

Characterization of inhibitory domains. As shown in Fig. 2, divergent residues are localized to two distinct regions within the D proteins: amino acids comprising  helix 1 and the C terminus comprising loop 6 and the tip of  helix 7 in the atomic structure (2, 3, 11, 21). To determine whether one or both of these domains confer inhibitory effects, chimeric genes were constructed and the proteins were expressed in vivo. The X/3 protein contains  helix 1 from X174; the remaining part of the protein is derived from 3. The opposite construct (3/X) contains  helix 1 from 3, with the remaining portion of the protein derived from X174 (Fig. 2). Table 1 shows that neither chimeric gene can complement a nullD mutant. However, inhibition of the wild type was observed, suggesting that the chimeric proteins retain enough function to enter into the morphogenetic pathway. Expression of the chimeric protein possessing the X174  helix 1 inhibits X174 morphogenesis (Table 1) even under low induction conditions and weakly inhibits 3 morphogenesis. Likewise, expression of the construct with  helix 1 from 3 strongly inhibits 3 morphogenesis but weakly inhibits X174 morphogenesis. These results suggest that both domains confer some level of inhibition.

View larger version (46K): [in this window] [in a new window]   FIG. 2.   (A) Sequence alignment of the X174 and 3 external scaffolding proteins. (B) Generation of the X/3 chimeric gene. (C) Verification of the X/3 chimeric gene.

Isolation of a X174 mutant resistant to the expression of the X/3 chimeric external scaffolding protein. To isolate a X174 mutant that could efficiently propagate in the presence of the chimeric X/3 D protein, 10⁸ wild-type phage were plated on the cells expressing the chimeric protein at high induction conditions. The chiD^r mutant was identified by its ability to consistently plate with EOPs near 1.0 in the presence of the chimeric protein. Initially 80% of the chiD^r genome was sequenced. Only one base change was found. The mutation confers a valinealanine change at amino acid 286 in protein A. This region of gene A does not overlap gene B. To confirm that this base change was solely responsible for the ChiD^r phenotype, the mutant A gene was moved into another wild-type background. The phenotype bred true.

2

In vivo analysis of wild-type inhibition by foreign and chimeric scaffolds. The location of the chiD^r mutation in gene A suggests that the X/3 protein does not block procapsid formation but blocks DNA packaging. To test this hypothesis, the assembly intermediates synthesized in wild-type X174-infected cells were analyzed by sucrose gradient sedimentation as described in Materials and Methods. The sedimentation profiles of ³⁵S-labeled viral products are presented in Fig. 3. In cells expressing the X174 D protein, infectious virion (114S) and degraded procapsid structures (70S) were detected. However, in the presence of the chimeric X/3 protein, only procapsids (108S) and degraded procapsids were present. This result is consistent with a block in DNA packaging and the location of the chiD^r mutation in gene A, since the A protein must interact with the procapsid during DNA packaging. In cells expressing the 3 external scaffolding protein, only low levels of procapsids and/or degraded procapsids were detected, suggesting that morphogenesis is inhibited before procapsid formation.

View larger version (21K): [in this window] [in a new window]   FIG. 3.   Sedimentation analysis of particles produced in the presence of foreign and chimeric scaffolding proteins. Symbols: , wild-type 3 grown in cells overexpressing the 3 D protein (control); , wild-type X174 grown in cells overexpressing the 3 D protein; , wild-type X174 grown in cells overexpressing the X3 chimeric D protein.

Isolation of a X174 multiple mutant resistant to the expression of the 3 chimeric external scaffolding protein. Although it was not possible to isolate a X174 mutant resistant to expression of the 3 external scaffolding protein via one mutational step (data not shown), a mutant was obtained in a multistep selection. Cells expressing the 3 external scaffolding protein were infected at a multiplicity of 0.01 in liquid culture. Induction conditions were kept at levels that produced an EOP of 10² in plating assays. The ForD^r (foreign D resistance) phenotype also confers resistance to both chimeric scaffolding proteins. The entire forD^r genome has been sequenced. Two single-base insertions have been found at the end of gene C, which is adjacent to gene D. One insertion creates a premature stop codon in gene C, three codons upstream from the natural stop codon (Fig. 4). The natural stop codon overlaps with the gene D start codon. The premature stop codon overlaps with the ribosome binding site (RBS) of gene D. C protein termination and D protein initiation may now be more efficiently coupled than in the wild type. In addition, the insertion may create an RBS that can more easily anneal to the 16S rRNA. The second insertion is found between the D gene RBS and start codon. These considerations suggest that part of the ForD^r phenotype may involve increasing wild-type D protein, as opposed to a truncated C protein, synthesis.

View larger version (13K): [in this window] [in a new window]   FIG. 4.   Nucleotide sequence of the upstream D regulatory region in the forD mutant.

	DISCUSSION

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Foreign external scaffolding proteins are potent cross-species inhibitors of viral morphogenesis. The Microviridae scaffolding proteins have diverged only 26% on the amino acid level, and this divergence is localized to two distinct regions:  helix 1 and loop 6/ helix 7 in the atomic structure. Chimeric proteins were designed to separate these two domains in order to determine their individual inhibitory effects. The results of this analysis suggest that both domains are responsible for some level of inhibition and demonstrate the feasibility of using closely related proteins as antiviral agents.

The efficiency of inhibition ascribed to each domain differs and appears to be concentration dependent. Low levels of induction are sufficient when the inhibited virus and  helix 1 of the chimeric protein are of the same origin. This suggests that  helix 1 may play a critical role in the self-association of D proteins into dimers or dimer recognition of other structural proteins. Within the atomic structure of the procapsid, four D proteins associate with the viral coat protein. The D proteins are arranged as asymmetric dimers of dimers, D1D2 and D3D4, and are not related by quasi-equivalence (2, 3). Residues between  helices 2 and 6 mediate the vast majority of the intradimer and interdimer contacts. This region of the X174 and 3 D proteins exhibits 96% conservation on the amino acid level. This high level of conservation most likely allows interspecies oligomerization, effectively diluting the amount of indigenous protein capable of progressing through assembly as homodimers. However,  helix 1 mediates some intradimer contacts, but only in the D1D2 species. Without these contacts, dimers with a foreign  helix 1 may not be efficiently placed into the D1D2 position. However, once placed into the D1D2 position, that helix makes no contacts with other scaffolding or structural proteins.

Some coat-scaffolding interactions may not be apparent in the current atomic structure. During crystallization, the X174 procapsid matured at the threefold axes of symmetry, producing a closed structure. The native, or open, structure has pores at the threefold axes. Genetic data (4, 7) suggest that an interaction may occur between D4  helix 1 and  helix 4 of the viral coat protein. Both helices are found at the threefold axis of symmetry in the closed structure. In an open structure, the coat protein helix could be shifted upward and might contact  helix 1 of the D4 subunit, which is the most closely associated with underlying coat protein. Both helices are amphipathic and could interact via hydrophobic interfaces. If this interaction is indeed present in the native structure, this may exclude dimers with a foreign  helix 1 from the D3D4 position.

Finally, contacts made by  helix 1 may be important in forming an assembly naive dimer, from which the D1D2 and D3D4 are formed. All three models are consistent with the high-level induction conditions needed for proteins with a foreign  helix 1 and the absence of large particles. Further analyses will be required to distinguish between these models. The isolation of the resistant mutants described here is limited. The selection procedures would not have generated mutations within the cloned foreign or chimeric genes. Considering the low level of inhibition conferred by chimeric scaffolding proteins with foreign  helices 1, it should be possible to construct the chimeric gene directly within the X174 genome and select for mutants which can propagate without concurrent expression of a cloned wild-type gene.

The mechanism of inhibition conferred by foreign COOH termini, on the other hand, is more apparent. The X174 chiD^r mutant alters viral protein A, a component of the genome biosynthesis/packaging machinery, which binds the procapsid along the twofold axis of symmetry. The location of the chiD^r mutation and the isolation of procapsids from cells expressing the chimeric protein suggest that chimeric and wild-type scaffolding proteins form procapsids that cannot interact with the genome biosynthetic/packaging machinery. Because the chiD^r phenotype does not circumvent the need for the wild-type protein, chimeric and wild-type proteins can achieve conformations needed for chiD^r A protein recognition that chimeras alone cannot achieve. Nor does the mutation confer resistance to the expression of the wild-type 3 D protein or the 3/X chimera, consistent with a model in which foreign scaffolding proteins confer multiple blocks in morphogenesis.

In the COOH termini, only the regions comprising loop 6 and  helix 7 have diverged between the X174 and 3 proteins.  helix 7 is ordered only in the D4 subunit, in which it makes multiple contacts with the underlying coat. While the D protein amino acids participating in these contacts have diverged, albeit conservatively, the target residues in the X174 and 3 coat proteins are identical. These observations suggest that the highly divergent loop 6 may be responsible for recognizing the genome biosynthetic/packaging machinery or forming part of its docking interface. With the identification of the chiD^r mutations, the chimeric D gene can be placed directly into a chiD^r background. The chimeric virus can be propagated in cells expressing the wild-type D protein. A direct and simple selection, the loss of complementation-dependent growth, can be used to isolate mutants that can utilize only the chimeric protein or a mutated form.