INTRODUCTION

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Virus attachment to the host cell is the first stage of the virus replication cycle. It requires the molecular interaction between the virion surface and a host cell receptor and is often the basis for viral species and tissue tropisms as well as virulence properties. The cellular receptors for some viruses have been defined and reveal diverse strategies for virus attachment, ranging from binding to specific cell surface proteins to attachment via widely distributed carbohydrate moieties, such as sialic acid and heparan sulfate (for a review, see reference 48). For a large number of viruses, however, specific host cell receptors have not been identified. The use of multiple receptors on individual or different cells could be one reason for this lack of knowledge. This scenario has been proposed for the attachment of flaviviruses (35), a genus of approximately 70 mainly arthropod-borne, enveloped RNA viruses. Most flaviviruses replicate in vertebrate and arthropod cells and exhibit a wide species and tissue tropism. Numerous candidate receptor proteins with a molecular mass of 40 to 80 kDa have been found to associate with flaviviruses in binding assays (19, 22, 27, 36, 44). Furthermore, an important role of heparan sulfate has also been demonstrated for the attachment of dengue-2 virus to vertebrate cells (7). Interestingly, cell surface glycosaminoglycans (GAGs) are exploited as attachment molecules by other viruses in a process thought to concentrate virus particles at the cell surface for subsequent binding to high-affinity receptors (for a review, see reference 2). No experimental evidence on the use or nature of a high-affinity receptor for any of the flaviviruses exists at present.

Flavivirus attachment and entry are mediated by the envelope (E) protein (~50 kDa), the major glycoprotein on the flavivirus particle (for reviews, see references 6 and 33). The E protein forms an oligomer with the small membrane (M) protein (~8 kDa) and constitutes most of the accessible virion surface; this is reflected in the dominance of the E protein as target antigen for virus-neutralizing and protective antibodies (33). The definition of the crystal structure of the ectodomain of the E protein of the flavivirus tick-borne encephalitis virus (TBE) (37), in combination with phenotypic analyses of E protein variants, has shed light on functional domains and mechanisms involved in flavivirus attachment and entry (for a review, see reference 33). An investigation by one of us on genotypic changes associated with host cell adaptation of the encephalitic flavivirus Murray Valley encephalitis virus (MVE) suggested an important role for residue 390 in the E protein in cell tropism and virulence (25). Asp₃₉₀ found in the prototype virus was altered to His, Gly, Ala, or Asn after passage of MVE in a human adenocarcinoma (SW13) cell line, resulting in improved growth in the human cell line as well as virulence attenuation in mice. Residue 390 in the E protein of MVE is part of an Arg-Gly-Asp (RGD) sequence, the integrin-binding motif important in cell-extracellular matrix and cell-cell adhesion (42). This evidence prompted the first proposition for the location of the flavivirus receptor-binding site in a conserved, hydrophilic domain encompassing residue 390, with a possible role for integrins in the attachment of some flaviviruses (25). However, the RGD motif is not found in the E protein of all flaviviruses: it is found in Japanese encephalitis virus (JEV) (31), yellow fever virus (YFV) strain 17D (39), and the related RGE/T sequences in other members of the JEV serocomplex (5, 8, 47), but the corresponding amino acids in the E protein of the dengue viruses are unrelated (12, 26, 30) and deleted in TBE (29). Intriguingly, the RGD sequence in the vaccine (17D) strain of YFV (39) arose as a consequence of host cell adaptation of the virulent Asibi strain, which has the corresponding amino acids Thr-Gly-Asp (11). Based on these sequence comparisons, it is unlikely that integrins are a general receptor for flavivirus attachment, in contrast to foot-and-mouth disease virus (16, 34) and coxsackievirus (40), which display a strict dependence on RGD-mediated integrin binding for host cell entry.

The crystal structure of the TBE E protein strongly supports a function in receptor binding of the hydrophilic region which harbors the RGD motif of some flaviviruses. This sequence is found in a solvent-exposed (FG) loop located in the immunoglobulin-like domain III of the E protein, and mutations which affect host cell tropism and virulence in different flaviviruses map in this region (37). In the current study, we introduced substitutions at the RGD motif in MVE with the use of an infectious clone. The phenotypic effects of changes in the putative flavivirus receptor-binding domain on virus growth, attachment, and entry in cultured cells and virulence in mice were examined.

	MATERIALS AND METHODS

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Viruses and cells. The MVE prototype strain MVE-1-51 (9) has been passaged 15 times in suckling mouse brain; a culture supernatant from C6/36 cells was used as working stock.

Plasmids. DNA fragments comprising the 5' or 3' half of the genome of MVE-1-51 were obtained by reverse transcription (RT)-PCR using expand reverse transcriptase and the Expand high-fidelity PCR system (both from Roche Diagnostics, Castle Hill, NSW, Australia). Oligonucleotide primers used in RT-PCR were designed to incorporate the promoter sequence for T7 RNA polymerase plus an ApaI restriction endonuclease recognition site (underlined) at the 5' terminus (5'-GCTTGGGCCCTAATACGACTCACTATAAGACGTTCATCTGCGTGAGCTTCCGATCTCAG-3') and a NotI site (underlined) at the 3' terminus (5'-TATGCGGCCGCAGATCCTGTGGTCTTCTCCCCCAT-3') (nucleotides in italics are not present in the viral genome). Two additional internal primers corresponded to sequences encompassing a unique BamHI site (underlined) in the middle of the genome (sense, 5'-GGAACTTCAGGATCCCCAATAGTCAATAGC-3'; antisense, 5'-GCTATTGACTATTGGGGATCCTGAAGTTCC-3'). RT-PCR fragments 5,057 and 6,024 bp in size and corresponding to the 5' and 3' portions of the genome, respectively, were produced. Plasmids pM110, pM210, and pM310 were generated by ligation of independently produced 5' 5,057-bp fragments, after digestion with ApaI and BamHI, into plasmid pBR322* (2,793 bp) digested with the same enzymes and treated with shrimp alkaline phosphatase (Roche Diagnostics). This vector was derived from pBR322 by deletion of a 1,666-bp fragment encoding tetracycline resistance between the EcoRI and BspEI sites and insertion of the polylinker from pBluescript KS+ (Stratagene, La Jolla, Calif.) at the site of deletion. Plasmids pM116, pM211, pM212, pM213, and pM215 contain the full-length MVE cDNA. They were generated by ligation of independently produced 3' 6,024-bp fragments cut with BamHI and NotI into pM110 or pM210 digested with the same enzymes. Plasmids pM312' and pM312" were produced by replacement of the MVE 5' terminus of clone pM212 with a 1,971-bp ApaI-XbaI fragment from pM310 or the adjacent sequence of 3,073 bp with an XbaI-BamHI fragment from pM310, respectively.

Introduction of mutations at codon 390 in the E protein gene. A shuttle vector, pM212(X/B), was made by cloning a 3,073-bp XbaI-BamHI fragment from pM212 into pBluescript II KS+ and subsequent deletion of the polylinker region between ApaI and BamHI by digestion with the two restriction endonucleases, treatment with T4 DNA polymerase to create blunt ends, and religation. To produce a Gly₃₉₀ mutant, a 563-bp PstI-SacI fragment from pM212(X/B) was exchanged with that obtained from RT-PCR amplification of nucleotides 1,593 to 5030 in the genome of MVE-1-51 passage variant P4/10 (25). The mutation was subcloned into pM212 by exchange of a 3,073-bp XbaI-BamHI fragment with that containing the mutated E protein 390 codon.

Transcription of RNA from full-length MVE cDNA clones. Plasmids containing the full-length MVE cDNA were digested with NotI and treated with Klenow fragment (Pharmacia Biotech) to create blunt ends. After extraction with phenol-chloroform and ethanol precipitation, linearized DNA was added to a transcription mix containing 0.5 U of T7 RNA polymerase (Promega, Madison, Wis.) and 1 U of RNase inhibitor (RNAGuard; Pharmacia Biotech, Uppsala, Sweden) per µl, 40 mM Tris-HCl (pH 7.9), 6 mM MgCl₂, 2 mM spermidine, 10 mM NaCl, 10 mM dithiothreitol, 1 mg of bovine serum albumin (BSA) per ml, 1 mM CAP analogue mG(5')ppp(5')G (Pharmacia Biotech), and 1 mM each ATP, UTP, and CTP. The mixture was incubated at 37°C for 5 min, GTP was added to 1 mM, and the incubation was continued at 37°C for 1 h. RNA transcripts were used directly in transfection experiments.

Transfection of BHK-21 and C6/36 cells. Transfection of BHK-21 cells was done by electroporation. Subconfluent cell monolayers were harvested with trypsin, washed twice in serum-free EMEM, and suspended in EMEM at 1.2 × 10⁷ cells/ml. The cells (10⁷) were mixed with in vitro-synthesized RNA in an electroporation chamber (0.4-cm gap; Life Technologies, Rockville, Md.) and subjected to two consecutive pulses at 250 V, 800 µF, and "low-ohm" setting using the Cell-Porator electroporation system I (Life Technologies). Cells were left at room temperature for 5 min, suspended in 24 ml of EMEM-5% FCS, and transferred to culture dishes for incubation at 37°C. Culture fluids were harvested after 3 days for plaque isolation and titration. For RNA transfection of C6/36 cells, lipofectin (6 µl; Life Technologies) was mixed with 0.4 ml of phosphate-buffered saline (PBS) and held at room temperature for 15 min, and then RNA transcript (0.2 µg) was added, and the mixture was applied to cells in a 35-mm dish (washed twice with PBS). After incubation at room temperature for 20 min, the transfection mix was removed, monolayers were washed once with PBS, and EMEM-7% FCS was added.

Virulence assays. Virulence assays were performed in 3-week-old Swiss White outbred mice as described (32). Virus stocks used in virulence assays were obtained by plaque purification on Vero cells followed by two rounds of amplification in C6/36 cells. Virus samples were serially diluted in Hanks' balanced salt solution (HBSS) containing 20 mM HEPES (pH 8.0) plus 0.2% BSA (HBSS-BSA), and 30 µl was inoculated into each animal by the intracranial (i.c.) or intraperitoneal (i.p.) route.

Infectivity assays. Vero cells (2 × 10⁵/well) and SW13 cells (10⁵/well) in 24-well Linbro dishes (ICN Biomedicals, Aurora, Ohio) were infected at ~0.1 PFU/cell, the virus was left to adsorb for 1 h at 37°C, and monolayers were washed twice with PBS before EMEM-5% FCS (1 ml) was added. At 16, 20, 24, and 28 postinfection (hpi), the culture supernatant was collected and stored at 70°C following the addition of HEPES buffer (pH 8.0) to 10 mM. Monolayers were washed once with PBS, and fresh medium (1 ml) was added. Virus growth samples from Vero and SW13 cells were titrated by plaque formation on Vero or SW13 cell monolayers, respectively, using six-well Linbro trays (ICN Biomedicals). Overlay medium for plaque assay contained EMEM plus 1% agar (Difco Laboratories, Irvine, Calif.) and 2% FCS in both cases. Plaques on Vero cells were visualized by adding neutral red stain (ICN Biomedicals; 0.02% [wt/vol] in 1% agar solution) at 4 to 5 days postinfection (dpi). Plaques on SW13 cells were visualized at 5 dpi after removal of agar overlay and staining with crystal violet (0.1% in 20% ethanol-H₂O).

Virus attachment and entry assays. For uptake assays, SW13 cell monolayers (60% confluent) in six-well trays were used after replacing the medium with HBSS-BSA. Virus samples (200 SW13 PFU in HBSS-BSA) were added to each well at room temperature. At specified times after virus addition, monolayers were washed three times with acid EMEM (pH 4.0, buffered with 20 mM [2-N-morpholino]ethane-sulfonic acid; ICN Biomedicals). The monolayers were incubated with acid medium for 2 min, and the medium was aspirated and replaced with HBSS-BSA. The acid treatment inactivates bound and unbound virus but not virus which has undergone uptake by endocytosis (10, 18). Overlay agar was then added, and cells were incubated for 5 days to allow plaque development. A control monolayer for each virus sample was used for plaque titration in the absence of the acid treatment.

Heparin inhibition of virus attachment. The effect of heparin on MVE infection of Vero, SW13, and BHK-21 cells was assayed by preincubation of virus samples (100 to 200 PFU in 100 µl of HBSS-BSA) with heparin (20 to 200 µg/ml; Sigma, St. Louis, Mo.) for 15 min prior to addition to cell monolayers (incubated with 200 µl of HBSS-BSA containing 20 to 200 µg of heparin per ml). After 1 h, agar overlay medium (see above) was added to allow plaque development.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Full-length infectious cDNA clones of MVE-1-51. cDNA fragments corresponding to the 5' and 3' halves of the MVE genome and overlapping at a unique BamHI site at nucleotide 5010 were generated by RT-PCR. A 5' ApaI restriction site, a T7 RNA promoter sequence, and a 3' NotI site were incorporated into the PCR primers to allow cloning of PCR fragments into a low-copy-number plasmid (pBR322*) and in vitro synthesis of full-length RNA transcript. These transcripts contain an authentic 5' end but six additional nucleotides (NotI site) at the 3' end (Fig. 1). Five full-length MVE cDNA clones (pM116, pM211, pM212, pM213, and pM215) comprising two of the three independently derived 5' halves and five independently derived 3' halves of the genome were generated. Two additional clones were produced from the third 5' half of the MVE genome by replacement of the MVE 5' end of clone pM212 with a 1,971-bp ApaI-XbaI fragment (pM312') or the adjacent sequence of 3,073 bp with an XbaI-BamHI fragment (pM312"). All plasmids encoding the full-length MVE cDNA were stably propagated in E. coli MC1061.1 cells but required incubation for 30 h at 37°C for sufficient yield.

View larger version (29K): [in this window] [in a new window]   FIG. 1.   Full-length infectious cDNA clone of MVE-1-51. Plasmid pM212 contains the full-length cDNA of MVE-1-51 (11,013 nucleotides), which includes an open reading frame (ORF) encoding a polyprotein of 3,434 amino acids (solid line). Restriction sites used in subcloning and genetic manipulations are shown (numbering is from the 5'-terminal nucleotide in MVE-1-51). Sequences at the 5' (plus an ApaI site and T7 promoter sequence) and 3' (plus a NotI site) termini of the viral genome are shown (boxed). The vector pBR322* is a deleted version of pBR322 (see Materials and Methods). The ampicillin resistance gene (Ap), the ROP gene, and the origin of DNA replication (ORI) are shown. NCR, noncoding region.

MVE mutants with substitutions at residue 390 in the E protein. Asp₃₉₀ in the MVE E protein is part of an RGD motif in the putative flavivirus receptor-binding domain. It is subject to replacement with other amino acids as a result of host cell adaptation of MVE and is also a determinant for virulence in mice (25). To investigate the impact of single amino acid changes at this site on cell tropism, virus entry, and virulence in the context of an otherwise identical genetic background, the codon for Asp₃₉₀ in MVE full-length clone pM212 was mutated to that for one of 10 other amino acids. A random mutagenesis approach was used which relied on a degenerate oligonucleotide to introduce randomized codons at residue 390 as well as silent substitutions generating a unique SacII site. A Gly₃₉₀ mutant was produced by substituting a cDNA fragment encompassing codon 390 in pM212 with that obtained by RT-PCR amplification from a variant virus (P4/10) which had been selected by passage in SW13 cells (25). The collection of E protein 390 mutants obtained is shown in Table 2. It includes the conservative replacement of Asp₃₉₀ with Glu and the nonconservative substitutions with small aliphatic residues (Gly or Ala), aliphatic hydrophobic residues (Val or Leu), an aliphatic polar residue (Thr), an aromatic polar residue (Tyr), an acidic amide (Gln), a polar weakly basic residue (His), and a basic residue (Lys). A clone unchanged at Asp₃₉₀ but with silent mutations introducing the SacII site was also generated.

Growth of E protein 390 mutants in vertebrate and invertebrate cells. A previous investigation demonstrated that the substitution of Asp₃₉₀ with His in the E protein of MVE resulted in altered virus growth in Vero and SW13 cells (25). Although the nucleotide sequences of the E protein genes of the passage variant and prototype virus were otherwise identical, the presence of differences elsewhere in the genome could not be excluded. To confirm that residue 390 is part of an E protein domain that functions as a determinant of host cell tropism and to extend this study to a wide range of amino acid substitutions at residue 390, ten 390 mutants with otherwise identical genetic backgrounds were examined for growth in Vero, SW13, and C6/36 cells. Infections were performed at 0.1 PFU/cell in one-step growth experiments. Virus titers of growth samples were determined on the corresponding cell lines by plaque (Vero and SW13) or immunofluorescence focus assays and (C6/36).

View larger version (28K): [in this window] [in a new window]   FIG. 2.   Growth of E protein 390 mutants in cell culture. Vero, SW13, and C6/36 cells were infected with MVE-1-51, the Asp390 control virus, or 1 of 10 E protein 390 mutants at multiplicity of ~0.1, determined for each cell type. Unbound virus was removed after 1 h of adsorption, and growth medium was added. At 16, 20, 24, and 28 hpi, supernatants were collected from Vero and SW13 cells for titration of virus infectivity, in the respective cell types. For growth assays in C6/36 cells, supernatant samples were taken at 24, 28, and 44 hpi, and virus titers were determined as focus-forming units (FFU) by immunofluorescence in C6/36 cells. Results for Vero and SW13 growth assays are from two separate experiments (shown in different graphs).

Effect of residue 390 in the E protein on entry kinetics into SW13 cells. The entry kinetics into SW13 cells of the His₃₉₀ mutant, which showed improved growth in this cell line, was compared to that of the Asp₃₉₀ control virus. Following incubation of virus on cell monolayers at room temperature for 5, 15, 30, and 60 min, unbound virus was removed and bound but noneclipsed virus was inactivated by the addition of acidic (pH 4.0) medium. Agar overlay medium was added, and plaques were allowed to develop over 5 days. The acid wash induces an irreversible conformational change in the E protein (1) which inactivates virus infectivity (10, 18). This was corroborated in control experiments, in which plaque formation was completely inhibited when adsorption was performed at 4°C for 1 h prior to the addition of acid medium. The kinetics of virus entry of the His₃₉₀ mutant was significantly faster than that of the Asp₃₉₀ virus, with greater than twofold differences seen at all time points (Fig. 3). Similar results were obtained in two repeat experiments. These data suggest that amino acid substitutions at residue 390 can result in more efficient virus uptake into SW13 cells. Consequences of enhanced uptake kinetics would be (i) a faster growth kinetics and (ii) an increase in the relative infectivity in the cell line used to generate the single-step growth curves. Collectively, these factors are likely to account for the improved growth phenotypes of most E protein 390 mutants observed in SW13 cells.

View larger version (18K): [in this window] [in a new window]   FIG. 3.   Uptake of MVE into SW13 cells. The kinetics of uptake of the Asp390 control and His390 mutant viruses into SW13 cells was determined as described in Materials and Methods. Percent uptake for each time point is expressed as the ratio of the number of plaques obtained after acid treatment and the number of plaques obtained on a control monolayer in the absence of acid treatment.

Differential inhibition of virus infectivity by heparin is determined by amino acids at residue 390 in the E protein. The improved entry kinetics into SW13 cells of one of the E protein 390 mutants relative to the control virus, the selection of amino acid changes at residue 390 during host cell adaptation of MVE (25), and the location of residue 390 in a domain with putative receptor-binding function (25, 37) suggest a critical role for this site in virus attachment to host cell receptors. Given that host cell adaptation of some other viruses correlated with an increased dependence on GAGs in virus attachment (3, 21, 43) and that a role for GAGs in dengue-2 virus attachment to vertebrate cells has been documented (7), we tested the effect of heparin on infection of Vero, SW13, and BHK cells with prototype MVE and E protein 390 mutant viruses.

View larger version (37K): [in this window] [in a new window]   FIG. 4.   Inhibition by heparin of virus infectivity in Vero, SW13, and BHK cells. (A) The Asp390 control and His390, Gly390, and Lys390 mutant viruses (200 PFU) were incubated with heparin (0, 50, 100, and 200 µg/ml) prior to their addition to cells pretreated with HBSS-BSA containing heparin at 0, 50, 100, or 200 µg/ml. Agar overlay was added to allow plaque formation after 1 h of adsorption at 37°C. Inhibition by heparin was calculated using the formula [(plaque number on nontreated cells  plaque number on heparin-treated cells)/(plaque number from non-treated cells)] × 100. (B) Inhibition of infectivity by heparin (200 µg/ml) of 10 E protein 390 mutants was assayed in parallel with the Asp390 control virus as in panel A. The percent inhibition by heparin of the Asp390 virus is subtracted from that for the E protein 390 mutants obtained in the same experiment. Results (mean and standard error) from three independent experiments are shown for each mutant. (C) Inhibition by heparin of virus binding to Vero and SW13 cells. The Asp390 control virus and the His390 mutant were incubated with heparin (0, 20, 50, 100, or 200 µg/ml) for 15 min at 4°C and then added to chilled Vero or SW13 cells treated with heparin (0 to 200 µg/ml). After 1 h of adsorption at 4°C, cell monolayers were washed, and agar overlay was added to allow plaque development. Percent inhibition by heparin is calculated as in panel A. Results (mean and standard error) from two independent experiments are shown.

Heparin inhibits entry of MVE into Vero and SW13 cells. To confirm that heparin inhibits virus binding or entry into cells, an entry assay was performed (Fig. 4C). Ice-cold mixtures of virus (Asp₃₉₀ control or His₃₉₀ mutant virus) and heparin (20 to 200 µg/ml) were added to chilled Vero or SW13 cell monolayers and held for 1 h at 4°C for binding to occur without uptake by endocytosis. We found that the effect of heparin on virus infectivity was markedly increased when virus adsorption was performed at 4°C and the heparin-treated virus inoculum was completely removed prior to the addition of an agar overlay (compare Fig. 4A and C). The infectivity of the His₃₉₀ mutant was inhibited by more than 50% at 20 and 200 µg of heparin per ml in SW13 and Vero cells, respectively. In contrast, the inhibition by heparin of entry of the Asp₃₉₀ control virus resulted in a plateau of not greater than 50% reduction of infectivity in both cell lines, suggesting that the Asp₃₉₀ virus can enter these cell lines by a mechanism which does not involve virus binding to cell surface GAGs. It is notable that Vero cells were significantly less susceptible than SW13 cells to inhibition by heparin in both binding and infectivity assays (Fig. 4A and C), suggesting a greater dependence on GAGs of MVE entry into SW13 than Vero cells.

Virulence of E protein 390 mutants. Mouse virulence of full-length MVE clone (pM212)-derived virus vM212 was determined by inoculating 3-week-old Swiss outbred mice with 10-fold serial dilutions of plaque-purified virus stock i.c. or i.p. (24, 32). Comparison of i.c. versus i.p. 50% lethal dose (LD₅₀) values, which did not differ by more than 10-fold, showed that vM212 was virulent (Fig. 5). The Asp₃₉₀ virus which was used as a control for the other infectious clone-derived variants was also virulent by this criterion. Of the six E protein 390 mutants tested for mouse virulence, the Glu₃₉₀, Gln₃₉₀, and Thr₃₉₀ mutants were also considered virulent. The Gly₃₉₀ and His₃₉₀ mutants were attenuated, as they induced no mortality by i.p. inoculation despite having i.c. LD₅₀ values (<50 PFU) similar to that of the Asp₃₉₀ virus. The Tyr₃₉₀ mutant showed an approximately 100-fold difference between the i.c. and i.p. LD₅₀ values and is considered intermediate in virulence.

View larger version (22K): [in this window] [in a new window]   FIG. 5.   Virulence of infectious clone-derived MVE and E protein 390 mutants. Virus stocks were diluted 10-fold serially in HBSS-BSA and used to inoculate 21-day-old Swiss outbred mice in groups of five by the i.p. or i.c. route. Mortality was recorded for 14 days.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Functional role of the putative flavivirus receptor-binding domain in cell tropism and attachment/entry. We have examined the functional role of the putative flavivirus receptor-binding domain in virus entry, host cell tropism, and pathogenesis in mice. In the E protein of MVE, this domain contains the RGD integrin-binding motif. The codon for Asp₃₉₀, which is part of the RGD motif, was targeted by site-directed mutagenesis using a full-length cDNA clone. Infectious clone-derived control virus vM212 was indistinguishable from the prototype virus, MVE-1-51, in growth in Vero cells and virulence in mice. All E protein 390 mutants were viable and produced plaques in Vero, SW13, and BHK-21 cells; however, marked differences in growth properties were observed. Replacement of Asp₃₉₀ with small aliphatic (Gly or Ala) or a weakly basic (His) amino acid resulted in the most significant changes in virus growth; a 50- to 100-fold reduction in growth in Vero and C6/36 cells and >10-fold enhancement of growth in SW13 cells was seen in comparison with an Asp₃₉₀ control virus. The other mutants showed smaller differences, but growth in Vero cells was always less efficient than that of the Asp₃₉₀ virus and comparable or improved in SW13 cells (Table 2). The observation that MVE is largely tolerant to a wide range of changes at the third amino acid of the RGD motif suggests that these changes do not alter the structure of the E protein such that replication in cultured cells is inherently impaired. This contrasts with the neighboring Gly₃₈₉, which, when replaced with Ala, destabilizes the structure of the E protein in YFV 17D (49). A Gly is found at the corresponding position in almost all flaviviruses, whereas Asp₃₉₀ (MVE E protein sequence numbering) is not highly conserved. A comparison of the efficiencies of RNA synthesis of wild-type and E protein 390 variants has revealed no significant difference when identical numbers of cells were infected (A. Thompson and L. Dalgarno, personal communication).

Role of GAGs in entry of an encephalitic flavivirus. Heparan sulfate on the cell surface plays a role in the attachment of a number of viruses (3, 21, 41, 43), including that of dengue-2 virus, to mammalian cells (7, 15). GAG-mediated virus attachment is inhibited by heparin, a highly sulfated form of heparan sulfate. We tested whether infectivity of MVE in Vero, SW13, and BHK-21 cells was sensitive to inhibition by heparin and found a dose- and cell type-dependent effect for the prototype virus. This inhibitory effect of heparin on virus infectivity was significantly greater for three of the 390 mutants (Gly₃₉₀, His₃₉₀, and Ala₃₉₀). Two mutants (Val₃₉₀ and Leu₃₉₀) showed an intermediate phenotype of susceptibility to heparin, whereas the others did not differ markedly from the Asp₃₉₀ virus (Table 2). The observation that substitutions at residue 390 in the E protein can render MVE infectivity highly susceptible to heparin inhibition strongly suggests that this site is in close proximity to a GAG-binding domain in the E protein and can directly influence the interaction between virus and cell surface GAGs. Support for this interpretation has been obtained in an investigation of JEV variants adapted to growth in SW13 cells which had single amino acid change located in a loop directly opposing residue 390 according to the crystal structure of the TBE E protein (unpublished data). Nevertheless, it cannot be excluded that substitutions at residue 390 in the MVE and JEV E proteins alter the overall conformation of the protein and influence a distantly located domain important for virus attachment and entry. The reason why small uncharged (Gly and Ala) or small polar (His) amino acids at residue 390 would enhance virus binding to GAGs is not immediately apparent. GAG-binding domains are characteristically rich in basic amino acids which are flanked by hydrophobic residues (41). An attractive hypothesis is that the positively charged Arg in the RGD motif or other basic residues in a region spatially proximal in the protein structure bind to negatively charged GAGs and that this interaction is subject to steric and charge hindrance depending on the amino acid at residue 390.

Effect of GAG dependence in virus entry on virulence. Residue 390 in the E protein of MVE is also a determinant of pathogenesis in mice. Of six mutants tested, two (Gly₃₉₀ and His₃₉₀) had lost neuroinvasiveness and were considered fully attenuated by the criteria of the virulence assay. However, their growth in the brain following i.c. inoculation was not notably changed, based on the average time to death of the infected animals. The loss of neuroinvasiveness of the Gly₃₉₀ and His₃₉₀ mutants most likely reflects their inefficient replication in extraneural tissues. Thus, the correlation of altered cell tropisms and of increased susceptibility of virus entry to inhibition by heparin with the type of amino acid at residue 390 extends to virulence phenotypes in mice. A parallel may be drawn between the attenuated 390 mutants of MVE and Sindbis virus variants, which were derived from cell culture adaptation, attenuated, and dependent on GAG-mediated attachment for infectivity in cultured cells (3, 4, 21). It was argued that the loss of virulence of the Sindbis virus variants was a consequence of their high affinity for GAGs and that after peripheral inoculation these attenuated variants are concentrated and inactivated in the liver, an organ highly enriched in GAGs, thus limiting their ability to spread to the brain. A similar scenario has been proposed for the attenuation of foot-and-mouth disease virus (43) and may explain the loss of neuroinvasiveness of E protein 390 mutants of MVE and other flavivirus variants attenuated by host cell adaptation.