This study was supported by grants from the National Institute of
Diabetes and Digestive and Kidney Diseases, National Institutes of
Health, to N.S. (DK53929) and F.S. (DK48265).
We thank Brian Klahn for expert technical assistance, Cristina Cueto
for help with plasmid construction, Michail Zaboikin for providing
IRES-containing constructs and the 3T3 cell line, Kathleen Schell and
Janet Lewis of the flow cytometry facility at the University of
Wisconsin
Madison and David McFarland of the HHMI Flow Cytometry
Facility at Vanderbilt University for help with running and analyzing
samples, Kendra Tutsch and staff of the Analytical Laboratory for the
use of the spectrophotometer and ELISA readers, Chinnasamy Jagannath
for the THP-1 cell line, Antonito Panganiban for the D17 cell line,
Didier Trono for pMD.G, David Camerini for pCDM8-luc, and David Rekosh
and Marie-Lou Hammarskjöld for generously sharing numerous
plasmid constructs, including ones with CTE. The following reagents
were obtained through the AIDS Research and Reference Reagent Program,
Division of AIDS, NIAID, NIH: 293 from Andrew Rice,
HeLa-CD4-LTR-
-gal from Michael Emerman, and pSV-A-MLV-Env from
Nathaniel Landau.
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