INTRODUCTION

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Epstein-Barr virus (EBV) is a human gamma-herpesvirus widely spread in the adult human population. This virus is associated with several malignancies such as Burkitt's lymphoma, nasopharyngeal carcinoma, Hodgkin's disease, gastric carcinoma, breast carcinoma, and B- and T-cell lymphomas and induces the permanent proliferation (immortalization) of quiescent B lymphocytes in vitro. In EBV-associated tumors in vivo as well as in EBV-infected B cells proliferating ex vivo, entry into a productive cycle is a rare event and the transcription of the EBV genome is usually restricted to a few genes defining a latent state of the viral cycle (for reviews, see references 25 and 36). Although the molecular events occurring during the switch from latency to the productive cycle are now partially understood for in vitro-immortalized B cells (42), the functions of many EBV gene products expressed during the lytic cycle have only partially been characterized, and very little is known about certain others. Among these, the early nuclear protein EB2 (7), which is also called M or SM (8), was originally described as a promiscuous transcription factor, as it activates transient expression of the chloramphenicol acetyltransferase (CAT) gene placed under the control of many different promoters (26). We reported recently that EB2 could activate cytoplasmic accumulation of unspliced RNAs, particularly when they are poor substrates for spliceosome assembly, which suggested an effect of EB2 on either splicing or RNA export or both (4). A recent report, using heterokaryon assays, has revealed that EB2, like its herpes simplex virus type 1 (HSV-1) homologue ICP27, has properties of an RNA export protein, i.e., nuclear-cytoplasmic shuttling and RNA binding activities, although no specific responsive RNA sequences have been identified so far on EB2 target RNA (38, 39). This was very reminiscent of the lentivirus Rev protein that mediates active nuclear export of intron-containing viral mRNA after binding to a cis-acting sequence on the RNA called the Rev response element (RRE) (for a review, see reference 34). Rev-dependent nuclear export of RNA requires at least two domains of the human immunodeficiency virus type 1 (HIV-1) protein: a highly basic N-terminal domain that also specifies nuclear-nucleolar localization and a short C-terminal leucine-rich nuclear export signal (NES). Similar leucine-rich NESs have now been found in many other viral and cellular proteins (for a review, see reference 17). These NESs are transferable to heterologous proteins, and mutations in the Rev NES impair both Rev shuttling and Rev-dependent export of RNA (12, 27, 46). Furthermore, it is now well documented that nuclear export of Rev is mediated by a complex trimolecular interaction involving the NES, the importin -like protein Crm-1 (also called exportin 1) and ranGTP, a small GTPase in its GTP-bound state (13). The elucidation of the role of Crm-1 in this process comes from experiments performed by Wolff and coworkers (47) showing that the antibiotic leptomycin B (LMB), which was reported to interact specifically with Crm-1, was a potent inhibitor of Rev nuclear export. However, the Crm-1-dependent pathway is not unique, and other export routes that are not sensitive to LMB exist. Indeed, NESs that do not belong to the growing family of leucine-rich NESs have now been described for different proteins such as TAP (1), hnRNPK (30), hnRNPA1 (29), and HuR (10).

It was recently published that the EBV protein EB2 contains a leucine-rich region (LRR) that could fit the consensus for leucine-rich NES (39). It was reported subsequently that the EB2-mediated export of intronless RNA and the intracellular localization of EB2 could be mediated by a direct association with Crm-1 (3). Our current observations lead to different conclusions. In this report, we have used a functional assay originally devised to assess the effect of Rev in exporting intron-containing RNA. In this Rev-controlled assay, EB2 efficiently stimulated the nuclear export of intron-containing RNAs. Our results clearly demonstrate that, under conditions where Rev-mediated export of intron-containing RNA is inhibited by inactivating the Crm-1-dependent pathway using LMB, the EB2-mediated export of intron-containing RNA is unaffected. Moreover, although a leucine-rich NES-like sequence has been identified in EB2, we show here by both site-directed mutagenesis and domain swapping that this domain is not required for nuclear export of intronless and intron-containing RNA and is therefore not a leucine-rich NES. Finally, in cell fusion experiments, we report that EB2 nucleocytoplasmic shuttling is LMB resistant. Therefore, our results suggest that the herpesvirus protein EB2 is involved in nuclear export of RNA through a mechanism distinct from what is used by the lentivirus Rev protein.

	MATERIALS AND METHODS

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Plasmids. Plasmid pCAT.RRE expresses, under the control of the cytomegalovirus promoter, a two-exon, one-intron precursor RNA in which the CAT gene and the RRE are located within the intron (see Fig. 1A). pCAT.RRE was made by subcloning the pDM128 (20) Bgl2-XbaI fragment into the pAAC plasmid linearized by BamHI and XbaI (4). pCAT.XRE is similar to pCAT.RRE except that it contains the human T-cell leukemia virus type 1 XRE sequence instead of the RRE. pAAC.CAT expresses the CAT protein tagged with the Flag epitope (IBI Flag system; Eastman Kodak). pSG5Flag.EB2 and pSG5Flag.EB2Cter have been constructed by ligating the BamHI-ClaI fragment (corresponding to EB2 amino acids 17 to 481) and the XbaI-ClaI fragment (corresponding to EB2 amino acids 185 to 481) of pAACFlag.EB2 (4), respectively, into pSG5Flag (11). pAACFlag.EB2L/A and pSG5Flag.EB2L/A encode an EB2 mutant in which leucines at position 234 and 236 have been changed to alanines. pAACFlag.EB2L/A was constructed by PCR mutagenesis as published in reference 19, using primers CTGGCCTCTGCCACCGCCGAGCCCATCCAAGACCCG and CGGGTCTTGGATGGGCTCGGCGGTGGCAGAGGCCAG. The PCR-amplified fragment was digested by ApaI and SphI and ligated into plasmid pAAC cut with ApaI and SphI. pAACFlag.M1 is a control plasmid derived from pAACFlag.EB2 by inserting two stop codons downstream of the BMLF1 AUG (4). A DNA fragment isolated by a two-hybrid yeast interaction trap encoding an N-terminally truncated version of Nup214 (amino acids 1754 to 2090) was cloned into pSG5Flag to generate pSG5Flag.can (11). Plasmids pSG5Flag.Rev and pSG5Flag.M10 expressing the tagged versions of Rev and Rev mutant M10, respectively, have been described previously (11). In pSG5Flag.RevEB2, the DNA sequence coding for the Rev NES region (amino acids 73 to 83) has been replaced by a synthetic DNA fragment encoding the EB2 LRR (amino acids 226 to 236) identified by Semmes and coworkers (39). This fragment was generated by hybridizing the deoxyoligonucleotides TGAGGTCACCTTGCCCAGCCCCCTGGCCTCTCTGACT and TCTAGAGTCAGAGAGGCCAGGGGGCTGGGCAAGGTGACC and by cloning this double-stranded DNA fragment into plasmid pSG5Flag.Rev digested with EspI and XbaI.

Transfections and CAT assays. Plasmids used for transfection were prepared by the alkaline lysis method and purified through two CsCl gradients. HeLa cells were grown at 37°C in Dulbecco modified Eagle medium (Life Technologies) supplemented with 10% fetal calf serum and were seeded at 8 × 10⁵ cells per 100-mm-diameter petri dish 10 h prior to transfection. Transfections were performed by the calcium precipitate method as described previously (4). To evaluate CAT protein expression, we used the Roche CAT enzyme-linked immunosorbent assay (ELISA) kit. As indicated in the figure legends, either 24 or 48 h after transfection, cells were collected in phosphate-buffered saline (PBS) and divided in half. Half of the cells were treated according to the manufacturer's instructions, and the other half were used to monitor protein expression. Western blotting was performed using the chemiluminescent ECL detection system purchased from Amersham Pharmacia Biotech. Relative amounts of protein detected by autoradiography were quantitated using the ImageQuant software from Molecular Dynamics.

RT-PCR analysis. Cytoplasmic RNA (5 µg) was reverse transcribed with oligo(dT) using Superscript II reverse transcriptase in a final volume of 20 µl as described by the manufacturer (Life Technologies). PCR was performed as described in reference 4 using 2 µl of cDNA and ³²P-labeled dCTP. When indicated, 20 and 25 PCR cycles were performed in order to make the PCR more quantitative. For plasmid pRcCMV-Na, the PCR was performed with primer GTAGTAACAGAGGAAATGC and primer GTAGGTCTATGTATTCAGCG to detect the BBRF1 RNA. For plasmid pUC128SV, primer CATTTGCTTCTGACACAACTG and primer GTGCAGCTCACTCAGTGTGGC, located in the first and second exons of the human -globin gene, respectively, were used to detect the globin RNA. For pCAT.RRE plasmid, primer GCATGATGAACCTGAATCGC, located in the CAT coding sequence, was used instead of oligo(dT) to initiate cDNA synthesis. The same primer in conjunction with primer CGTTGATATATCCCAATGGC was used to detect the CAT RNA by PCR. To control our PCR experiments, endogenous expression of the -actin mRNA was evaluated by reverse transcriptase PCR (RT-PCR). Primer GCTGCGTGTGGCTCCCGAGGAG and primer ATCTTCATTGTGCTGGGTGCCAG were used in the PCR to amplify a 690-bp DNA fragment corresponding to the -actin mRNA.

Heterokaryon assays. HeLa cells were transfected in 100-mm-diameter petri dishes by the calcium precipitate method as described earlier. Twenty-four hours posttransfection, the precipitate was washed and cells were trypsinized. Approximately 200,000 HeLa cells were seeded on glass coverslips with an equal number of NIH 3T3 cells in 35-mm-diameter dishes and allowed to grow overnight at 37°C. Cells were then treated for 2 h with 100 µg of cycloheximide per ml to inhibit protein synthesis and 25 nM LMB (kindly provided by B. Wolff) when inhibition of Crm-1-dependent protein export was required. Subsequently, cells were washed in PBS and heterokaryons were formed by incubating the coverslips for 2 min in polyethylene glycol 3000-3700 (Sigma), 50% in PBS. Following cell fusion, coverslips were washed extensively in PBS and returned to fresh medium containing 100 µg of cycloheximide per ml and 25 nM LMB when needed. After 0.5 to 2 h at 37°C, cells were fixed with 4% paraformaldehyde and the immunofluorescence assay was performed essentially as described previously (11) except that either EB2 polyclonal antibody or monoclonal anti-Flag and anti-hnRNPC (4F4; kindly provided by G. Dreyfuss) antibodies were used and that Hoechst 33258 (Sigma) was added at 5 µg/ml during the secondary antibody incubation.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

EB2 induces the cytoplasmic accumulation of intron-containing RNA expressed from an HIV-1 Rev reporter gene. EBV EB2 and HIV-1 Rev both have been shown previously to increase the nuclear export of incompletely spliced RNAs which are poor substrates for splicing (4, 6, 43). However, these observations were made in different systems, and in the case of Rev, an RNA cis-acting element, the RRE, was found to be necessary for activity. In order to further understand the molecular mechanisms of EB2 function, we took advantage of an assay which has been extensively used to study Rev activity. The reporter plasmid that we used, pCAT.RRE, is a derivative of pDM128 (Fig. 1A) (20). When transfected into HeLa cells, pCAT.RRE expressed a two-exon, one-intron pre-mRNA which is mostly spliced, resulting in the excision of the CAT gene and very low levels of CAT protein expressed (Fig. 1B, lane 1). As described previously, Rev expression induced the cytoplasmic accumulation of unspliced RNA and, therefore, an increase in the amount of CAT protein detected (Fig. 1B and D, lane 2). Conversely, the M10 Rev mutant, in which a mutation inactivates the NES, has no effect on the basal level of CAT protein synthesis (Fig. 1B, lane 3). When a plasmid expressing EB2 was cotransfected with pCAT.RRE, the amount of CAT protein detected dramatically increased and reached a level similar to what could be seen in the presence of Rev (Fig. 1B, lane 4). EB2Cter, an EB2 N-terminal deletion mutant, appeared to be incompetent in transactivating this reporter gene, suggesting that the amino terminus of EB2 is required for full activity (Fig. 1B, lane 5). It is noteworthy that maximum transactivation by Rev and EB2 was obtained with different amounts of transfected plasmids, 100 ng of the Rev-expressing construct and 250 ng of the EB2 expression construct (data not shown).

View larger version (20K): [in this window] [in a new window]   FIG. 1.   Cytoplasmic accumulation of intron-containing RNA mediated by Rev and EB2 proteins. (A) Schematic view of the reporter plasmid pCAT.RRE and the proteins used in this assay. The Arg/X/Pro repeated motif (RXP) and the LRR of EB2 are indicated. Flag.EB2Cter is an EB2 mutant having the N-terminal region deleted. The sequence of the wild-type Rev NES is given as well as that of the M10 mutated version. All the proteins are tagged with the Flag peptide. (B) HeLa cells were transfected with reporter plasmid pCAT.RRE or pCAT.XRE and with plasmids expressing the Rev and EB2 proteins as indicated. CAT protein accumulation was measured in cell lysates 48 h after transfection using a CAT ELISA. The relative amounts of CAT protein expressed were given as percentages of the amount obtained for EB2. (C) Expression of the EB2 and Rev proteins in these experiments was evaluated by Western blotting using a chemiluminescent ECL detection system. The relative amounts of protein were evaluated on autoradiographic films using the ImageQuant software from Molecular Dynamics. The values are given in arbitrary units as follows: 43 (lane 2), 38 (lane 3), 106 (lane 4), 73 (lane 5), 35 (lane 7), 28 (lane 8), 82 (lane 9), and 62 (lane 10). (D) Rev and EB2-mediated cytoplasmic accumulation of CAT RNA was monitored by RT-PCR analysis of cytoplasmic RNA from the same transfection experiment. The cellular -actin mRNA was used as an internal control. To obtain more quantitative results, each PCR was performed using different amplification conditions (20 and 25 cycles).

The LRR in EB2 is not a leucine-rich NES. Having shown that EB2 is able to induce cytoplasmic accumulation of intron-containing RNA expressed from pCAT.RRE, we decided to take advantage of this Rev-controlled system to study the mechanism of EB2-mediated nuclear export of RNA. In a recent publication, Semmes and coworkers reported, using the heterokaryon assay, that EB2 is able to shuttle between the nucleus and the cytoplasm (39). Furthermore, they have pointed out that an LRR showing strong homology with various leucine-rich NESs could specify EB2 nuclear export (Fig. 2A). An extensive mutagenesis in the Rev protein has revealed that changing any one of the three leucines of the NES (L78, L81, and L83) to alanines resulted in a complete loss of activity (27). In order to test whether the putative NES was important for EB2 function, we generated a mutant, EB2L/A, in which the last two leucines (L234 and L236) were changed to alanines (Fig. 2A). If the LRR is indeed the EB2 NES, this mutation should completely abolish the effect of EB2 on CAT mRNA export. On the other hand, if the LRR includes the EB2 NES, it should be able to substitute for the Rev NES in the context of the Rev protein. Consequently, we have also constructed a Rev mutant, called Rev/EB2, by replacing the Rev NES with EB2 amino acids 225 to 237 encompassing the LRR (Fig. 2A). These mutants were tested for their ability to transactivate the reporter plasmid pCAT.RRE. As demonstrated in Fig. 2B, Rev efficiently increased the nuclear export of unspliced RNA (Fig. 2B, compare lanes 1 and 2), but the RevM10 NES mutant (lane 3) and the Rev/EB2 hybrid protein (lane 4) failed to do so, indicating that the EB2 LRR is unable to substitute for the Rev NES. Using the same assay, we show that both EB2 (lane 6) and the LRR mutant EB2L/A (lane 7) efficiently exported unspliced RNA. Another Rev/EB2 mutant that included an extended version of the EB2 LRR (amino acids 221 to 240) similarly failed to activate our reporter gene (data not shown). As shown by Western blotting (Fig. 2B, lower panel), the amount of proteins expressed validated the results drawn from the CAT experiment. However, it is possible that EB2 activates gene expression by different mechanisms depending on the substrate RNA; thus, the EB2L/A mutant could be fully active in one system and inactive in another. To test this hypothesis, we compared the effects of EB2 and of the LRR mutant using two other EB2 reporter constructs, pUC128SV and pRcCMV-Na (4). pUC128SV contains a thalassemic allele of the human -globin gene (Fig. 2C). The -thalassemic gene contains a G-to-A transition at position 1 in the first intron (IVS1), which causes the activation of three cryptic 5' splice sites, otherwise completely silent in the wild-type precursor RNA (Fig. 2C). Upon transient transfection of plasmid pUC128SV in HeLa cells, RT-PCR analysis showed that the three cryptic 5' splice sites were used (Fig. 2D, lane 1). EB2 and the EB2L/A mutant (Fig. 2D, lanes 2 and 3) strongly increased the cytoplasmic accumulation of unspliced -thalassemic RNA and decreased the amount of spliced RNA as previously reported (4). We also used a construct called pRcCMV-Na from which the naturally occurring intronless BRRF1 EBV early RNA is expressed. As shown in Fig. 2D, lane 4, the intronless BRRF1 RNA could be detected by RT-PCR in the cytoplasm of transfected cells. Coexpression of EB2 resulted in an increase of the cytoplasmic accumulation of BRRF1 RNA, and this effect was clearly not affected by mutation of the EB2 LRR (Fig. 2D, lane 6). Collectively, our results strongly suggest that EB2 leucines 234 and 236 do not participate in EB2 function and indicate that the LRR is not a leucine-rich NES.

View larger version (29K): [in this window] [in a new window]   FIG. 2.   The EB2 LRR is not a bona fide leucine-rich NES. (A) Schematic representation of EB2 and Rev mutants. In Flag.EB2L/A, EB2 leucines 234 and 236 were changed to alanines. In Flag.Rev/EB2, the Rev NES was replaced by the EB2 LRR as indicated. (B) CAT protein expression was measured in HeLa cell lysates transfected by pCAT.RRE and plasmids encoding Rev and EB2 proteins as indicated. The values are given as percentages of the amount of CAT protein detected with Flag.EB2. Expression of the EB2 and Rev proteins was evaluated by Western blotting using an anti-Flag antibody. (C) Schematic representation of reporter genes from pRcCMV-Na and pUC128SV plasmids and of the RNA expressed from these vectors. Oligonucleotides used for the quantification of these transcripts by RT-PCR analysis are indicated by arrows. (D) Activity of Flag.EB2 and Flag.EB2L/A mutants evaluated by semiquantitative RT-PCR analysis of BRRF-1 and -thalassemic RNA expressed from plasmids pRcCMV-Na and pUC128SV. The same results were obtained when the PCRs were done with different number of cycles (data not shown).

EB2-associated nuclear export of unspliced RNA is LMB resistant. Having shown that the EB2 LRR was not a NES, we next asked whether another leucine-rich NES differing from the consensus could be present in the EB2 protein. Leucine-rich NESs are found in a variety of proteins. They are known to function via a direct interaction with an importin -like protein called Crm-1 in a ranGTP-dependent manner. The fungal metabolite LMB is known to specifically target Crm-1 and to block its interaction with both ranGTP and the NES. This in turn results in an inhibition of the protein nuclear export (13, 16). To test whether EB2-mediated nuclear export of intron-containing RNA was dependent on the Crm-1 export factor or not, transient transfections of HeLa cells using plasmid pCAT.RRE and Rev or EB2 expression vectors were repeated. Twelve hours after transfection, cells were washed and incubated for a further 6 h without or with 10 nM LMB. A 6-h incubation time was chosen because (i) the effect of LMB on Rev activation was strong at that time and (ii) we noticed that an incubation time of 16 h resulted in a dramatic reduction in the amount of EB2 and Rev proteins expressed (data not shown). As expected, Rev activation of CAT expression was dramatically reduced when LMB was added to the medium (Fig. 3B, compare lanes 1, 2, and 3). However, LMB appeared to have only a slight effect on EB2 activity (Fig. 3B, compare lanes 6 and 7). This slight effect was also seen with RevM10 (Fig. 3B, lanes 4 and 5) and with EB2Cter (Fig. 3B, lanes 8 and 9), which are inactive in RNA export. As shown by Western blotting (Fig. 3C), the amounts of proteins expressed in this experiment were not affected by LMB. Therefore, it appears that EB2 induces the nuclear export of intronless RNA by a Crm-1-independent mechanism.

View larger version (20K): [in this window] [in a new window]   FIG. 3.   LMB does not affect EB2-associated nuclear export of unspliced RNA. (A) Schematic representation of the LMB experiment. HeLa cells were transfected by the calcium phosphate method. At 12 h posttransfection, cells were washed with fresh medium supplemented with 20 nM LMB when indicated and incubated for a further 6 h. At this point, cell extracts were made and CAT protein was titrated using a CAT ELISA. (B) Relative CAT protein amount expressed in HeLa cells transfected with pCAT.RRE and plasmids expressing Rev and EB2 proteins as indicated. LMB treatment is indicated by a plus sign. (C) Detection of Rev and EB2 proteins in the same transfection experiment by Western blotting using an anti-Flag antibody.

EB2 nucleocytoplasmic shuttling is not inhibited by LMB. It has been previously published that, although EB2 localizes predominantly to the nucleoplasm, it shuttles continuously between the nucleus and the cytoplasm (39). To confirm and extend these observations, we have evaluated whether EB2 nucleocytoplasmic shuttling was sensitive to LMB. HeLa cells were transfected with plasmids allowing the expression of EB2 and Rev and then fused to mouse NIH 3T3 cells by the polyethylene glycol method. As shown in Fig. 4A, in HeLa/NIH 3T3 heterokaryons, EB2 was detected in the mouse nucleus after a 30-min incubation period (panel a). At 2 h postfusion, EB2 appeared to equilibrate between the mouse and human nucleus (panel c), as did Rev (panel e), indicating that both proteins are shuttling. As reported previously (47), Rev shuttling was strongly inhibited by LMB (Fig. 4A, panel f). However, we did not notice any effect of LMB on the ability of EB2 to relocate in the mouse nucleus (panels b and d), demonstrating that EB2 nuclear export was Crm-1 independent. As a control, we also looked at the endogenous human hnRNPC protein localization in the fused cells. As expected, hnRNPC, which carries a nuclear retention signal, was found restricted to the HeLa nucleus after a 0.5- or 2-h incubation time, as revealed by indirect immunofluorescence using a human-specific anti-hnRNPC antibody (panels a', c', b', and d'). Shuttling of the EB2L/A mutant was also tested in the heterokaryon assay. Cell fusions between NIH 3T3 cells expressing EB2L/A and HeLa cells were performed. As shown in Fig. 4B (panel h), EB2L/A shuttled between the mouse and human nuclei similarly to wild-type EB2.

View larger version (41K): [in this window] [in a new window]   FIG. 4.   Effect of LMB and mutations in the LRR on the shuttling of EB2 in interspecies heterokaryons. HeLa cells were transfected with plasmids coding for Rev, Flag.EB2, and Flag.EB2L/A mutants. HeLa cells were fused to NIH 3T3 cells in medium containing cycloheximide (100 µg/ml) and LMB (25 nM) when needed. After 0.5 to 2 h, cells were fixed and subjected to immunofluorescence with an anti-EB2 polyclonal antibody (a, b, c, d, g, and h) and anti-hnRNPC (a', b', c', d', g', and h') or anti-Rev (e and f) monoclonal antibodies. Cells were finally stained with Hoechst 33258 (e', f', g", and h") and fluorescein isothiocyanate-labeled anti-rabbit antibody (a, b, c, d, g, and h), fluorescein isothiocyanate-labeled anti-mouse antibody (e and f), or Texas red-labeled anti-mouse antibody (a', b', c', d', g', and h').

View larger version (48K): [in this window] [in a new window]   FIG. 5.   LMB does not relocate EB2Cter to the cell nucleus. HeLa cells were transfected with plasmids expressing Flag.Rev and Flag.EB2Cter. Twenty-four hours after transfection, cells were treated for 3 h with 10 nM (b and e) or 25 nM (c and f) LMB or not treated (a and d). Flag.Rev and Flag.EB2Cter localization was performed by immunofluorescence with an anti-Flag monoclonal antibody. Whereas Rev completely relocates to the cell nucleus upon LMB treatment (b and c), EB2Cter localization is not affected by high concentrations of LMB (e and f).

can, a transdominant negative mutant of Nup214, inhibits EB2 export pathway. Nuclear export of proteins and RNA occurs through the nuclear pore complex, a huge macromolecular structure of 125 MDa composed of about 100 proteins called nucleoporins. One of them, Nup214/can, is found in a multiprotein complex including Crm-1 (14). Nup214 has recently been implicated in the Crm-1-dependent pathway as well as in other nucleocytoplasmic transport pathways (13, 24, 45). Therefore, we decided to test whether Nup214 could be involved directly or not with EB2 nuclear export. can, a Nup214 C-terminal fragment including the phenylalanine glycine repeat (FG repeat)-rich region, has been previously described to have a transdominant negative phenotype and to inhibit Rev function in the CAT.RRE system (2, 23). However, when the RRE is replaced by the TAP binding sequence (CTE), TAP also induces the cytoplasmic accumulation of CAT-CTE intron-containing RNAs, but overexpression of can has no effect in this assay (2, 23). A similar can mutant was overexpressed in our CAT.RRE reporter system to determine whether it could affect EB2-mediated RNA export. As expected, can efficiently repressed Rev activity to about 10 to 20% of control level (Fig. 6A, lanes 3 and 4). Similarly, can expression resulted in a dramatic inhibition of EB2 transactivation, indicating that Nup214 could be involved in the EB2 export pathway (Fig. 6A, lanes 6 and 7). Inhibition of CAT.RRE RNA nuclear export by can was not due to a general effect on mRNA export since (i) can overexpression has only a slight effect on CAT expressed from pAAC-CAT, a basic CAT reporter plasmid (Fig. 6A, lanes 9 and 10), and (ii) endogenous -actin mRNA expression appeared to be insensitive to can overexpression as revealed by RT-PCR analysis using different amplification conditions (Fig. 6B). These observations therefore indicate that Nup214 participates in the nuclear export of the EBV protein EB2.

View larger version (35K): [in this window] [in a new window]   FIG. 6.   Overexpression of a transdominant negative mutant of Nup214/can impairs EB2-mediated nuclear export of unspliced RNA. (A) HeLa cells were transfected with reporter plasmid pCAT.RRE or pAAC.CAT and plasmids expressing Flag.EB2 or Flag.Rev when indicated. To evaluate the role of Nup214/can in the EB2-mediated export process, increasing amounts of plasmid expressing can (a transdominant negative mutant of Nup214/can) were included in the transfection mix (0.5 µg in lanes 3, 6, and 9 or 1 µg in lanes 4, 7, and 10). Twelve hours after transfection, CAT protein expression in HeLa cells was measured using a CAT ELISA. The values are given as percentages of the amount of CAT protein detected with Flag.EB2 (lanes 1 to 7) and with pAAC.CAT only (lanes 8 to 10). (B) The experiment was controlled by performing RT-PCR analysis on the endogenous cellular -actin mRNA. To obtain more quantitative results, each PCR was performed using different amplification conditions (20 and 25 cycles).

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

The experiments reported here clearly indicate that HIV-1 Rev and EBV EB2 proteins efficiently induce the nuclear export of an intron-containing RNA. It was reported previously that the HIV-1 tat/rev intron, present in the CAT.RRE RNA, was inefficiently removed due to suboptimal signals in the 3' splice site (43). Therefore, and as documented recently (4), our data confirm that EB2 has the ability to induce cytoplasmic accumulation of intron-containing RNAs when they are poor substrates for spliceosome assembly. Recently, EB2 was found to shuttle between the nucleus and the cytoplasm (reference 39 and this work), and a leucine-rich region with high homology to the Rev NES was identified in the protein primary sequence. Therefore, it was tempting to propose that EB2 nuclear export of RNA was, similar to Rev, dependent on the Crm-1 pathway (39, 40). To address this issue, we focused on the EB2 LRR and investigated the requirement for the Crm-1 protein in nuclear export of EB2 and its target RNA. As the Rev protein was included as a positive control, the CAT RRE reporter gene derived from pDM128 was found to be a valuable model to study EB2-dependent nuclear export. Our data indicate that (i) the EB2 putative leucine-rich NES is not an NES per se, as it could be mutated without affecting the function of EB2 and could not substitute for the Rev NES in the context of the Rev protein; (ii) EB2 transactivation is not affected by LMB (a Crm-1-specific inhibitor) under conditions where Rev activation is dramatically reduced; and (iii) EB2 nucleocytoplasmic shuttling visualized by an interspecies heterokaryon assay is also not LMB sensitive. Our results appear to be rather different from those which have recently been published by Boyle and coworkers (3). They have reported, by performing transient expression assays with lymphoblastoid B cells (BJAB), that (i) EB2 activation of an intronless CAT reporter gene is inhibited by LMB and potentiated by overexpression of Crm-1; (ii) complete deletion of the EB2 LRR resulted in an 80% reduction in transactivation, whereas point mutations of leucines in the EB2 LRR reduced activation by only 40%; and (iii) EB2 can be pulled down with Crm-1 in coimmunoprecipitation experiments. According to previous work on leucine-rich NESs, we believed that if the EB2 LRR (₂₂₇LPSPLASLTL₂₃₆) was the EB2 NES, it should be completely inactivated by mutating leucines 234 and 236 to alanines as exemplified for p53 (44), PKI (46), FMRP (15), IB (22), and Rev (27). However, we show that mutation of these leucines does not significantly affect EB2 transactivation, indicating that these residues are not essential for function. Furthermore, although Boyle et al. reported that complete deletion of the LRR (mutant LRR-) reduced EB2 activation to about 20% of wild-type EB2 levels, they also showed that mutant LRR- is insoluble in 1% Triton, suggesting a tight association with nuclear structures. We have previously described a similar EB2 deletion mutant called 7 lacking the LRR. We found that mutant 7 is not functional in different transactivation assays (4) and localizes, similarly to LRR-, to large nuclear dots (data not shown). We believe that the 7 mutant as well as most of our C-terminal deletion mutants is a nonfunctional activator (4), probably because it does not fold properly and aggregate in the nucleus to give rise to these large nuclear substructures, which are also observed by visible light microscopy (data not shown). Therefore, it is not surprising that the LRR- mutant is not fully active. The significant discrepancy between our results and those obtained by Boyle and coworkers could be also partially explained if EB2 contains more than one NES, one dependent on Crm-1 and active in both B lymphocytes and HeLa cells, and the other being Crm-1 independent and active only in HeLa cells. We also cannot rule out at this point the possibility that the LRR participates in the nuclear export of EB2 through a Crm-1-independent mechanism.

In agreement with Boyle and coworkers (3), we show that a negative transdominant mutant of Nup214, called can, is an efficient inhibitor of EB2 trans activation. This result suggests that Nup214 is an essential component of the EB2 export pathway. This last observation is not in contradiction to our observation that Crm-1 is not involved in EB2 nuclear export, since Nup214 was proposed to be involved in different export and import pathways (13, 24, 45). For example, the cellular TAP protein has been identified as the export factor for the CTE-containing RNA of type D retrovirus (18). Although TAP function is insensitive to LMB, it binds to Nup214 both in vitro and in yeast cells (23, 24). In conclusion, our results favor a mechanism for the nuclear export of EB2 distinct from the Rev pathway but also involving Nup214. The EB2 NES as well as the EB2 export pathway has now to be carefully identified and characterized.

The EBV EB2 protein is not unique in its capacity to affect mRNA nuclear export. Other herpesviruses express EB2-like factors, i.e., HSV-1 ICP27, human herpesvirus 8 ORF57, herpesvirus saimiri ORF57, bovine herpesvirus 4 HORF1/2, etc., that act posttranscriptionally to facilitate early and lytic viral gene expression. Although these factors have been shown to activate CAT reporter genes, their mechanism of action as well as their role in viral pathogenesis is still not clear. For HSV-1, it was shown that HSV-1 ICP27 null mutants did not replicate their DNA and were unable to grow in Vero cells (41). By use of temperature-sensitive mutants, ICP27 was found to simultaneously activate viral intronless genes and repress intron-containing ones. Furthermore, it is now well documented that ICP27 is a shuttling protein which activates nuclear export of intronless mRNA (32, 33, 38, 41). Export of ICP27 is mediated by an LRR located at the N terminus of the protein, but the ICP27 export receptor has not been identified yet (38). The EB2 protein is somehow different, since in our hands it does not inhibit expression of intron-containing genes but activates nuclear export of intron-containing polyadenylated RNA possessing suboptimal splice sites (reference 4 and this work). However, the mechanisms of action of these two proteins may not be so different. Indeed, it is believed that splicing is a prerequisite for nuclear export of most mRNAs. Accordingly, intron-containing RNAs are not normally found in the cytoplasm and very few RNAs, including histones, c-jun, alpha interferon, and hepatitis B virus RNA, do not contain introns. These particular RNAs are nevertheless exported to the cytoplasm, and for the histone H2a and the HSV-1 thymidine kinase, RNA sequences that induce efficient cytoplasmic accumulation of these intronless transcripts have been identified (21, 31). Therefore, poor expression of viral intronless RNA may be explained by the presence of cryptic splice sites that would allow nonproductive assembly of splicing factors. In the absence of RNA transport elements, such as those found in the histone H2a and the HSV-1 thymidine kinase RNA (21, 31), intronless RNA would be retained in the nucleus and eventually degraded. Interestingly, such a cryptic 5' splice site is present in the bacterial CAT gene used to study EB2 function (4, 39). Similarly, intron-containing transcripts with suboptimal splice sites are retained in the nucleus until fully spliced. EB2 and ICP27 proteins would efficiently interact with these nucleus-entrapped RNAs and promote their export to the cytoplasm, therefore competing with spliceosome assembly. In this respect, the data presented here are relevant to EBV biology since, as for HSV-1, most EBV early and late mRNAs are synthesized from intronless genes, whereas genes expressed during latency harbor introns. Furthermore, several intron-containing RNAs appear to accumulate in the cytoplasm of infected cells during the productive cycle (5, 28). We believe that, similarly to Rev and TAP, EB2 is a nuclear export factor that facilitates cytoplasmic accumulation of both intronless RNA and intron-containing RNA with suboptimal splice sites.

The question of the interaction of EB2 with its target RNA is also important to address. Although the extent of the EB2 effect is dependent on the RNA template, no specific EB2-interacting sequences have been identified so far on the RNA targets, and this is also true for ICP27. Different groups including ours have reported the binding of recombinant EB2 to various RNA probes in vitro (4, 37, 39). However, by Northwestern analysis we noted that RNA does not interact with full-length EB2 but with C-terminally truncated EB2 protein species (data not shown). We found this interaction to be mediated by the RXP region in vitro, but surprisingly, we also demonstrated that this domain was dispensable in vivo since it could be deleted without affecting EB2-mediated nuclear RNA export (4). Therefore, it is tempting to speculate that EB2 does not bind RNA directly in vivo but, instead, interacts with target RNA via an adapter protein that could be either hnRNP, SR proteins, or even components of the basal splicing machinery. This would explain why many different mRNAs are sensitive to EB2, including the CAT transcripts, but also cellular RNA, as the EB2 protein was reported to induce transformation of rodent fibroblasts by a mechanism which involves overexpression of the myc proto-oncogene (9).

Further work is now needed to precisely map functional domains in the EB2 protein and to identify cellular proteins directly involved in EB2 shuttling and EB2-mediated RNA export.