INTRODUCTION

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Heterosexual transmission of human immunodeficiency virus (HIV) infection occurs through mucosal surfaces and is the major route of infection worldwide. This mode of transmission is increasing in prevalence more rapidly than any other in the West (31, 33, 42). Male-female transmission rates (per contact infectivity estimated to be 0.0009 in North American women) are reported to be approximately eight times more efficient than female-male rates, with history of concomitant sexually transmitted diseases (STDs) being the most strongly associated risk factor (22). Conflicting data have been reported on the selective pressure of mucosal transmission on the phenotypic and genotypic characteristics of transmitted viral isolates from a heterogeneous inoculum. Predominant isolation from peripheral blood of virus able to infect both macrophages and CD4⁺ T lymphocytes (non-syncytium inducing [NSI], M-tropic) over those that preferentially infect T lymphocytes (syncytium inducing [SI], T-tropic), during or near to acute HIV infection have lead to the suggestion that NSI viruses may be more readily transmitted via mucosal routes (40). The mechanisms for such selective transmission of HIV isolates may be one of either selective penetration or selective amplification within the infected host (33). Analyses of genital biopsies from HIV-infected women and preliminary studies from this laboratory, using cervical organ culture as a model of primary infection, have demonstrated that HIV-infected cells reside within subepithelial mucosa, with no evidence of HIV infection of epithelial cells (20, 21, 23, 27, 35). Furthermore, it has been demonstrated that the primary targets of simian immunodeficiency virus (SIV) infection, following intravaginal infection of macaques, are cervical and vaginal subepithelial cells (35). Such data indicate that establishment of HIV infection requires transepithelial penetration. Whether intact genital epithelium presents a barrier to, or is an active participant in, HIV transmission has not been tested in primary human mucosal tissue.

Epithelium along the female genital tract differs in structural cellular organization: the vagina and ectocervix, the site most exposed to a natural inoculum, are composed of stratified epithelium, whereas the endocervix is composed of a single epithelial monolayer. Multiple mechanisms for HIV transmission across genital epithelia have been proposed: direct HIV infection of epithelial cells, transcytosis of HIV through epithelial cells, epithelial transmigration of HIV-infected donor cells, uptake of HIV by intraepithelial Langerhans cells, or circumvention of epithelium via breaches in epithelial integrity (14, 33). Evidence for HIV infection of, or transcytosis through, epithelial cells is derived from in vitro studies using epithelial cell lines, which may bear little relation to primary intact genital epithelium (1, 38). Furthermore, infection or transcytosis in such models is dependent on cell-associated virus, an observation at odds with efficient mucosal cell-free SIV or feline immunodeficiency virus infection (3, 35). Strong epidemiological association of inflammatory ulcerative venereal disease with HIV transmission and observation that mucosal SIV transmission may be enhanced following thinning of vaginal epithelium by progesterone implants suggests a barrier role for genital epithelium (18, 31). Furthermore, recent studies have demonstrated that STDs increase both the number of CD4 cells in genital mucosa and the expression of chemokine receptors known to function as HIV coreceptors, thereby increasing the number of target cells (16, 24, 30). While HIV may achieve transepithelial penetration by more than one mechanism, the relatively low incidence of per-contact infectivity suggests that this is unlikely to reflect a constitutive mechanism. However, male-female transmission is also influenced by factors relating to the male partner, including seminal viral load and incidence of STDs (31), all of which have an impact on contact infectivity.

While condoms provide an effective barrier against transmission of HIV and other STDs, they require the consent of the male partner, which cannot always be negotiated by women at risk for infection. Thus, there is an urgent need to develop prevention strategies that are under the personal control of women. The potential of effective topical vaginal virucides to prevent sexual transmission of HIV and other STDs is widely recognized (8). However, proper evaluation of the efficacy of such agents in blocking HIV infection of female genital tissue has been hampered by the lack of appropriate experimental models.

Thus, understanding the first critical events in genital mucosal transmission of HIV infection is important in developing strategies to block or limit such transmission. In this study, human genital mucosal tissue from premenopausal seronegative women was been used to define primary target cells for HIV infection within genital mucosa, differential susceptibility of such tissue to M-tropic and T-tropic HIV isolates, and the interaction of HIV with genital epithelium. Furthermore, this in vitro model has been used to determine the efficacy of potential vaginal virucides designed to protect women from HIV infection.

	MATERIALS AND METHODS

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Virus culture and infection. The following strains of HIV-1 were used in this study: BaL (grown in monocyte-derived macrophages), RF and IIIB (grown in H9 cells) (AIDS reagent project, National Institute for Biological Standards and Control, Potters Bar, United Kingdom), and SL-2, 2044, and 2076 (generously donated by Paul Clapham, Imperial Cancer Research Fund, United Kingdom) grown in phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cell (PBMC) cultures. Cell-free viral preparations were passed through 0.2-µm-pore-size filters and treated with DNase I (Sigma Ltd., Poole, United Kingdom) prior to use (100 U/ml, 5 mM MgCl₂, 30 min at 37°C). Chronically infected PM-1 T cells (AIDS reagent project) were produced by continual culture (RPMI 10% [RPMI 1640 medium supplemented with 10% fetal calf serum, penicillin, streptomycin, and L-glutamine]) of these cells following exposure to the BaL or RF strain of HIV-1. Chronically infected PBMC cultures were established following infection of PHA-stimulated PBMC cultures with HIV_BaL. Infection was monitored by p24 antigen release, measured by enzyme-linked immunosorbent assay (ELISA; DuPont NEN, Hounslow, United Kingdom) according to the manufacturers protocol. Chronically infected cells were treated with mitomycin C (200 µg/ml; Sigma) for 1 h at 37°C prior to use (38).

Culture of human genital tract tissue explants and HIV infection. Cervical and vaginal tissue were obtained from premenopausal women undergoing planned therapeutic hysterectomy at St. George's and St. Helier Hospitals, London, United Kingdom. Cervical and vaginal explants, comprising of both epithelial and stromal tissue, were produced using either 3- or 8-mm-diameter biopsy punches as described below. Intact explants (3 mm) were cultured on squares of stainless-steel mesh, individually, in 24-well flat-bottomed plates so that a meniscus of culture medium (Eagle's minimal essential medium supplemented with penicillin, streptomycin, L-glutamine, and HEPES buffer [EMEM], Sigma) was in contact with the under surface of the grid, as previously described (23). In some experiments, where indicated, explants were directly cultured in 300 µl of RPMI 10% in a 96-well plate. Explants were incubated at 37°C in a humidified atmosphere containing 5% CO₂, and two-thirds of the medium was changed every 2 to 3 days, taking care not to disturb any migratory cells within the culture wells. Two protocols were used for HIV infection of explant tissue. Initially, explants were cultured for 2 days prior to infection in either the absence or presence of PHA (5 µg/ml). Explants were then exposed to viral isolates by immersion in 1 ml of cell-free virus (10³ to 10⁶ 50% tissue culture infective doses [TCID₅₀]) for 2 h at 37°C. After incubation with infectious virus, explants were washed a minimum of five times in phosphate-buffered saline (PBS) and then cultured on grids as described above. Explants stimulated with PHA were cultured in the presence of interleukin-2 (IL-2; 10 U/ml; AIDS reagent project) throughout the experiment. After 8 days, explants were processed for immunohistochemistry or PCR. Remaining tissue was transferred to 96-well plates, cultured in medium (RPMI 10%) alone or restimulated by culture in the presence of PHA (5 µg/ml) for 2 days, and subsequently refed with medium containing IL-2. Culture medium was collected every 2 to 3 days and stored at 70°C before subsequent measurement of p24 antigen content by ELISA. For later experiments, explants were exposed to viral innoculum, by immersion in cell-free virus (10³ to 10⁶ TCID₅₀) for 2 h at 37°C, within 4 h of isolation. Subsequently explant tissue was washed in PBS as described above, and tissue was directly transferred to 96-well plates, cultured in medium (RPMI 10%) alone or stimulated by culture in the presence of PHA (5 µg/ml) for 2 days, and subsequently refed with medium containing IL-2. In both culture models, any migratory cells were retained within the culture wells through out the experiment.

Preparation of epithelial sheets and primary epithelial cultures. Cervical epithelial sheets were isolated from 8-mm biopsies following enzymatic digestion using dispase II (Boehringer Mannheim Ltd., Lewes, United Kingdom) and trypsin-EDTA (Sigma). Tissue was incubated in dispase overnight at 4°C. The following day, biopsies were washed three times in PBS to remove dispase and the epithelial cells were separated from the submucosa using fine forceps as previously described (15). Ectocervical epithelium (released as an intact sheet of cells) and endocervical epithelium (released as small clumps of cells) were placed in trypsin-EDTA for 20 min at 37°C with frequent agitation. The resulting cell suspensions were centrifuged and resuspended at 2 × 10⁵ ectocervical cells and 10⁵ endocervical cells per ml in keratinocyte-serum free medium (Gibco/Life Technology, Paisley, United Kingdom). Cells were grown in 12-well plates at 37°C in a humidified atmosphere containing 5% CO₂ and refed every 2 days by replacing half of the existing medium. Epithelial monolayers were demonstrated to be viable and functional by growth characteristics, structural and ultrastructural morphology (light and electron microscopy), mitotic activity [incorporation of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye], and presence of cytokeratin markers (confirming cell origin) [K13, stratified ectocervical cells; K18, columnar endocervical cells] [data not shown]). The intestinal epithelial cell line I407 (American Type Culture Collection) was cultured in RPMI 10%. Epithelial monolayers were exposed to cell-free virus (30 to 70 ng of HIV p24 per ml, pretreated with DNase) or chronically infected cells (pretreated with mitomycin C) for 2 or 24 h at 37°C in 5% CO₂. Epithelial monolayers were washed five times immediately after exposure to cell-free HIV or chronically HIV-infected cells and washed again 48 h later. Culture medium was collected every 2 days for p24 ELISA. Eight days postinfection, monolayers were processed for PCR or cocultured with PM-1 T cells, seeded at a 1:1 ratio for 10 days for virus isolation. PM-1 T cells are susceptible to both M- and T-tropic HIV, expressing both CXCR4 and CCR5 coreceptors.

Virus transmission across polarized epithelial sheets. Ectocervical epithelial sheets (8 mm), isolated as described above, were analyzed by light microscopy (×200) for any visible perforation of the epithelial cell surface. Viability and functional analysis of epithelial sheets was carried out as described above. In addition, presence of functional intercellular junctions (which exclude inulin) were demonstrated by the detection of inulin transfer following EGTA treatment. Epithelial sheets were clamped in diffusion chambers (12) consisting of two acrylic plates, each of which had a central aperture (3.5-mm diameter) connected to vertical chambers providing independent access to apical and basolateral surfaces. A leakproof seal was obtained by the means of concentrally positioned O-rings (6-mm diameter). Cell-free virus (30 to 70 ng of HIV p24 per ml, equivalent to 10⁵ TCID₅₀) or chronically infected cells in culture medium containing 0.5 mCi of [¹⁴C] inulin were added to the apical chamber (1 ml), while culture medium alone was added to the basolateral chamber, and polarized tissue was incubated for 2 h at 37°C in 5% CO₂. Culture medium was subsequently withdrawn from apical and basolateral chambers, and the epithelial tissue was removed. Medium was tested for the presence of [¹⁴C]inulin, p24 antigen, and infectious virus by coculture with PM-1 T cells. Epithelial layers were washed, either processed for quantitative PCR or fixed in 3% glutaraldehyde in PBS, and sent for processing for transmission electron microscopy (TEM) (Department of Anatomy, St. George's Hospital Medical School).

Measurement of tissue viability. Potential toxicity of virucidal agents was quantitated using the principle of MTT (Sigma) dye reduction by viable explant tissue into a methanol soluble formazan product. In brief, ectocervical explants (3-mm diameter) were incubated in virucidal agent (0.01 to 100 µg/ml) overnight and subsequently washed five times in PBS. Tissue was then either immediately tested for viability (T1) or cultured on grids in EMEM for a further 5 days before testing for viability (T6). To assess tissue viability, washed explants were incubated in medium containing MTT (250 µg/ml) for 3 h at 37°C. Tissue viability was determined by dividing the optical density of the formazan product at 570 nm by the dry weight of the explant. Toxicity was determined by comparison of viability between treated explants and untreated control tissue. Virucidal agents were considered to be nontoxic only at concentrations that demonstrated no reduction in tissue viability at either T1 or T2. A minimum of three independent experiments using tissue from separate donors were performed in duplicate for each condition.

Immunohistochemistry. Tissues were fixed overnight in neutral buffered formalin, and 4-µm sections of paraffin-embedded tissue were prepared. Endogenous peroxidase was inactivated in deparaffinizsed sections with a 30-min treatment in methanol-0.3% (vol/vol) H₂O₂. Antigens in paraffin sections were unmasked with a 10-min treatment with pronase (Dako Ltd., High Wycombe, United Kingdom). Slides were washed between incubations with Tris-buffered saline. Slides were incubated with 20% (vol/vol) rabbit serum in Tris-buffered saline for 30 min followed by overnight incubation at 4°C with one of the following primary monoclonal antibodies diluted in 20% (vol/vol) rabbit serum: anti-p24 (Kal-1), CD2 (MT910), CD68 (PGM1), CD1a (NA1/34), CD45RO (OPD4), and mouse immunoglobulin G1 (Dako) and major histocompatibility class II (WR18 [Serotec, Oxford, United Kingdom] and L243 [American Type Culture Collection). Biotinylated rabbit anti-mouse immunoglobulin G antibody (Dako) was applied to the sections, followed by avidin-biotin peroxidase complex (Dako) and diaminobenzidine (DAB) substrate (Sigma). Sections were counterstained with Mayer's hematoxylin. For dual immunohistochemistry with primary antibodies raised in the same species, the above procedure was applied in two consecutive treatments. Sections were not counterstained. The first monoclonal antibody was detected with the peroxidase-DAB method. The procedure was then repeated, starting at the pronase step, for the second monoclonal antibody which was detected with avidin-biotin alkaline phosphatase (Dako) and nitroblue tetrazolium salt and bromochloroindolylphosphate substrates (Sigma).

PCR for proviral DNA. For PCR, DNA was extracted from cells and tissues by an overnight digestion at 55°C and 5 min at 95°C with 500 µg of proteinase K per ml, 0.1% (vol/vol) Nonidet P-40 (Sigma), and PCR buffer II (Perkin-Elmer, Warrington, United Kingdom). Viral DNA was purified using QIAamp spin columns (Qiagen, Crawley, United Kingdom). Qualitative PCR for HIV-1 DNA was carried out by amplification of a 540-bp long terminal repeat LTR sequence (5) which had a sensitivity of 10 copies per 10⁵ cells. To control for sample quality and loading, duplicate PCRs were carried out using primers for -actin (forward primer, 5'-GAA GAT CAA GAT CAT TGC TCC TCC-3'; reverse primer, 5'-CTG GTC TCA AGT CAG TGT ACA GG-3') (GenBank accession no. M10277). Quantitative proviral PCR for HIV-1 LTR DNA was carried out by real-time PCR on a ABI Prism 7700 sequence detection system as previously described (9). The following reagents were used for this assay: LTR forward primer, 5'-CAC ACA AGG CTA CTT CCC TGA-3' (59 to 79); LTR reverse primer, 5'-TCT CTG GCT CAA CTG GTA CTA GCT T-3' (141 to 165); probe 5'-(6-carboxy-fluorescein [FAM])AGA ACT ACA CAC CAG GGC CAG GGA TCA G(6-carboxy-tetramethyl-rhodamine [TAMRA])-3' (85 to 112) (based on the reference sequence for HIV-1, isolate HXB2; GenBank accession no. K03455); -actin forward primer, 5'-TCA CCC ACA CTG TGC CCA TCT ACG A-3' (486 to 510); reverse primer 5'-AGT CAG TCA GGT CCC GGC C-3' (567 to 585); probe 5'-(VIC proprietary dye [PE Applied Biosystems])ATG CCC TCC CCC ATG CCA TCC TGC GT(Q) (TAMRA)-3' (GenBank accession no. AB004047). Primers and FAM-labeled probe were obtained from MWG Biotech, Ebersberg, United Kingdom, and VIC-labeled -actin probe was obtained from PE Applied Biosystems, Warrington, United Kingdom. -actin was used as an internal control allowing normalization of HIV copy number relative to the number of genome equivalents in the specimen. The threshold sensitivity was 10 DNA copies per reaction.

	RESULTS

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HIV infection of genital mucosa is potentiated by immune activation. Susceptibility of female genital tract mucosa to HIV infection was investigated in vitro using mucosal tissue explants, obtained from seronegative premenopausal women undergoing hysterectomy as previously described (23). Ectocervical, endocervical, and vaginal explants (3 mm³) were cultured under resting (medium alone) or activating (in the presence of PHA and IL-2) conditions. Explant tissue was exposed, in a nonpolarized manner, to primary NSI and laboratory-adapted SI HIV-1 isolates with known coreceptor restriction: BaL (NSI, CCR5/CCR3 restricted), IIIB, and RF (SI, predominantly CXCR4 restricted). Ectocervical and endocervical explant cultures were demonstrated to be susceptible to infection with HIV-1_BaL as indicated by increased accumulation of HIV p24 release independent of culture conditions (Fig. 1). In contrast, T-tropic strains of HIV (IIIB and RF) induced significant levels of productive HIV infection only in immune activated tissue. However, productive infection with either IIIB or RF could still be rescued if tissue stimulation (with PHA) was delayed by 8 days after exposure to HIV (Table 1). Persistence of HIV within explants for 8 days prior to stimulation was dependent on HIV infection, as these effects were inhibited in the presence of zidovudine. A similar pattern of HIV replication was also observed in vaginal explants (data not shown). To determine whether other primary isolates of HIV replicated similarly to HIV-1_BaL, ectocervical explants were exposed to primary isolates SL-2 (NSI, predominantly CCR5 restricted), 2044 (SI, CXCR4 restricted), and 2076 (SI, dualtropic, able to use CCR5, CCR3, and CXCR4). Explants were inoculated with virus within 4 h of obtaining tissue from surgery to minimize any potential changes in coreceptor expression and cultured in the presence or absence of activating conditions. Unlike HIV-1_BaL (at an equivalent dose [data not shown]), all three primary strains required immune activation to induce significant levels of viral replication (Fig. 2).

View larger version (19K): [in this window] [in a new window]   FIG. 1.   Kinetics of HIV replication in cervical explant tissue. (A) Accumulation of HIV-1 DNA in ectocervical explants was determined 7 days after infection with HIV-1BaL, HIV-1IIIB, or HIV-1RF as assayed for LTR DNA by quantitative real-time PCR as described in Materials and Methods. Explants either were prestimulated with PHA 2 days prior to HIV infection and subsequently cultured with IL-2 (filled bars) or were cultured in medium alone before and after infection (open bars). Data represents the mean and standard error of three independent experiments using paired explants from separate donors. p24 antigen release from ectocervical (B) and endocervical (C) explants was measured by ELISA. Explants were cultured either alone (open symbols, solid lines) or prestimulated with PHA (5 µg/ml) 2 days prior to viral exposure and restimulated 8 days post exposure. Stimulated explants were cultured in the presence of IL-2 (10 U/ml) (closed symbols, broken lines). Explants were exposed to HIV-1BaL (105 TCID50) without () or with () (PHA, HIV-1IIIB (106 TCID50) without () or with () PHA, or HIV-1RF (106TCID50) without () or with () PHA. Data represent the mean from a minimum of three independent experiments using paired explants from separate donors. (t test; *, P = <0.05; **, P = <0.01).

View larger version (11K): [in this window] [in a new window]   FIG. 2.   Replication of primary HIV isolates in cervical explant tissue. Ectocervical explants were exposed to HIV-1 isolates SL-2 (M-tropic, NSI, CCR5 restricted), 2044 (T-tropic, SI, CXCR4 restricted), or 2076 (dualtropic, SI, able to use CCR5, CCR3, and CXCR4) by immersion in cell-free virus (103 TCID50) for 2 h at 37°C. Subsequently explant tissue was washed in PBS, and tissue was directly transferred to 96-well plates, where they were cultured in medium alone (RPMI 10%) (open bars) or stimulated by culture in the presence of PHA (5 µg/ml) for 2 days and subsequently refed with medium containing IL-2 (filled bars). Data are shown as p24 antigen release (mean ± standard error) 14 days postinfection and represent the mean from a minimum of three independent experiments using paired explants from separate donors.

Primary target cells of HIV infection within genital mucosa. Mucosal explants were processed for immunohistochemistry 7 days after in vitro exposure to HIV isolates to determine the primary target cells of HIV infection within genital mucosa. In a previous study, we have described the normal cellular distribution of immune cells in such cultures (23). Such studies demonstrated that CD1a Langerhans cells are exclusively found in cervical eithelium, CD3 cells are predominantly detected in the mucosa, closely associated with the epithelium, and CD14/68-positive macrophages are restricted to subepithelial mucosa. Analysis of tissue infected with M-tropic HIV-1_BaL, in the absence or presence of stimulation, demonstrated numerous cells, within cervical subepithelial mucosa, positive for HIV p24 expression (Fig. 3A). The majority (>90%) of these cells were dual positive for p24 expression and the macrophage marker CD68 (Fig. 3C). Few p24 cells were positive for CD3 expression, and there was no difference in their frequency between activated or resting explants. Some p24-positive cells were detected in immune activated cervical tissue exposed to T-tropic HIV isolates (IIIB and RF); however, their frequency was far lower than that seen with M-tropic strain of BaL, and it was not possible to determine their phenotype (Fig. 3B). HIV-infected epithelial cells or CD1a-positive Langerhans cells were not detected by immunohistochemistry in any HIV-exposed explants; furthermore, in all experiments no p24-positive cells were ever detected in the epithelium regardless of their phenotype.

View larger version (71K): [in this window] [in a new window]   FIG. 3.   (A and B) Immunohistochemical staining of p24 expression (blue/purple) in an ectocervical explant 7 days after infection with HIV-1BaL (A) and HIV-1RF (B). (C) Dual staining for p24 expression (blue/purple) and macrophage marker CD68 (brown) in an ectocervical explant 7 days after infection with HIV-1BaL. Original magnification for all panels, ×400.

Lack of evidence for epithelial infection or transcytosis of HIV. Further investigations were carried out to exclude a low level of HIV infection of genital epithelial cells. In vitro cultures of primary ectocervical and endocervical monolayers were established based on previously described methods (37). Epithelial monolayers were exposed to either cell-free M-tropic HIV-1_BaL or T-tropic HIV-1_RF, or to cell-associated HIV in the form of PM-1 cells (a T-cell line expressing both CCR5 and CXCR4 coreceptors) or human PBMC, chronically infected with either BaL or RF. The human intestinal epithelial cell line I407, previously demonstrated to be susceptible to HIV infection and to transcytose HIV (1, 25) was used as a positive control. Primary cultures of ectocervical and endocervical epithelial monolayers, unlike I407 cells, were resistant to HIV infection with either cell-free or cell-associated M- or T-tropic virus (Table 2). Furthermore, no evidence of endocytosis was detected by TEM of primary ectocervical, endocervical, or vaginal epithelium following 6 and 24 h of exposure to either cell-associated or cell-free HIV (data not shown). In contrast, I407 cells were susceptible to infection with cell-free or cell-associated HIV-1_RF but resistant to HIV infection with either cell-free or cell-associated HIV-1_BaL. Furthermore, while transient adherence of lymphocytes to I407 cells was detectable (Fig. 4A), adherence of such cells to primary genital epithelium was either very infrequent or not detectable by either light or electron microscopic analysis of primary epithelial cultures (Fig. 4B and C).

View larger version (70K): [in this window] [in a new window]   FIG. 4.   (A) TEM showing lymphocyte adherence to I407 epithelial monolayer (original magnification, ×7,800). Experiments representative of a minimum of five explants for each condition. (B) TEM showing ultrastructure of stratified cervical ectocervical epithelium following exposure to PM-1 T cells infected with HIV-1BaL (original magnification, ×260). The epithelial sheet has been removed from underlying stroma following overnight treatment with dispase. Epithelial tissue was exposed to cell-associated virus for 2 h in the described diffusion chamber and gently washed before processing for TEM. There is no evidence of adherence or penetration of PM-1 T cells into the epithelium (experiment representative of three). (C) TEM showing single endocervical epithelial monolayer following exposure to PBMC infected with HIV-1BaL (original magnification, ×1,200). Epithelial cells contain multiple mucus-containing vesicles and express multiple microvilli on their apical surface. Endogenous mononuclear cells can be seen in the underlying stromal tissue. The epithelial tissue was exposed to PBMC infected with HIV-1BaL for 2 h and processed as described above. There is no evidence of adherence of paracellular migration of donor PBMC (experiment representative of five).

Polarized genital epithelium is impervious to cell-free or cell-associated HIV. To determine whether HIV can cross intact genital epithelium, epithelial sheets were polarized using a diffusion chamber allowing independent bathing of apical and basolateral epithelial surfaces as previously described (12). Such a system facilitated apical exposure of epithelial surfaces to HIV, as presented in vivo. Ectocervical stratified epithelial sheets were isolated following overnight digestion with dispase as previously described. Integrity of epithelial surfaces was assessed prior to experiments by direct light microscopic examination of the mucosal surface and permeability assessed during experiments by [¹⁴C]inulin diffusion. Polarized cultures of ectocervical epithelial sheets were exposed to cell-free or cell-associated HIV as described above. Measurement of basolateral p24 release and infectious coculture assays demonstrated that stratified epithelium, which excluded [¹⁴C]inulin, was impervious to cell-free or cell-associated HIV (Table 3). Furthermore, TEM analysis of polarized ectocervical and endocervical tissue exposed to either cell-free or cell-associated HIV demonstrated no evidence of HIV transcytosis or paracellular penetration of either cell-free HIV or HIV-infected cells. In addition, no interaction between exogenous HIV-infected mononuclear cells and epithelium was observed. Lack of paracellular penetration of cervical epithelial sheets by HIV-infected mononuclear cells was confirmed by demonstration that epithelial sheets were negative, by PCR, for proviral DNA following exposure to such infected cells (data not shown).

Vaginal virucides block HIV infection of genital mucosa. Further investigations were carried out to determine the efficacy of potential virucides to block HIV infection of cervical explants. Compounds included nonoxynol-9 (N-9; a nonionic surfactant known to be active against HIV and other sexually transmitted agents [36]), gramicidin (GD; a peptide antibiotic with virucidal activity [2]), and PRO 2000 (a naphthalene sulfonate polymer that appears to disrupt the initial binding and fusion events of HIV infection [32]). Agents were tested (range, 100 to 0.01 µg/ml) for potential tissue toxicity following overnight culture with ectocervical explants. Viability of cervical explants, assessed by MTT assay, was reduced following exposure to N-9 or GD at concentrations of >1 µg/ml, while PRO 2000 demonstrated no toxicity at 100 µg/ml (data not shown). As potential virucidal agents should demonstrate no tissue toxicity, which could reduce barrier effects of genital epithelium, for all subsequent investigations we used N-9 and GD at 1 µg/ml and PRO 2000 at 100 µg/ml. To determine the efficacy of these agents to block HIV infection of cervical tissue, explants were preincubated with virucides for 1 h prior to overnight exposure to virus in the continued presence of virucidal agent. Explants were exposed to virus in a nonpolarized manner, allowing direct access to both subepithelial and epithelial cells, analogous to conditions of compromised genital epithelium in vivo. Following overnight exposure to virus, explants were washed to remove any exogenous virus and virucidal agent and subsequently cultured for 10 days in the presence or absence of activating conditions prior to measurement of p24 production and proviral accumulation. Presence of N9, GD, and PRO 2000 during exposure of ectocervical explants to HIV-1_BaL (10⁵ TCID₅₀/ml) resulted in a 30%, 71%, and 97% inhibition of p24 production, respectively. PRO 2000 was the only agent to completely block proviral formation, as determined by PCR, and this agent was also able to efficiently block HIV infection with three other primary HIV strains, SL-2, 2044, and 2076, under activating conditions (PHA and IL-2) (Fig. 5).

View larger version (24K): [in this window] [in a new window]   FIG. 5.   Effect of vaginal virucides on HIV-infected genital mucosa. Cervical explants were preincubated with the virucidal agent N-9 (1 µg/ml), GD (1 µg/ml), or PRO 2000 (100 µg/ml) for 1 h prior to overnight exposure to HIV-1 strain BaL (; 105 TCID50/ml), SL2 (), 2044 (), or 2076 () (all 103 TCID50/ml) in the continued presence of the virucidal agent. Explants were exposed to virus in a nonpolarized manner, allowing direct access to both subepithelial and epithelial cells. Following overnight exposure to viral inoculum, explants were washed extensively in PBS, stimulated by culture in the presence of PHA (5 µg/ml) for 2 days, subsequently refed with medium containing IL-2, and cultured for 14 days. Data represent percent inhibition of p24 production at day 14 of culture, compared to control cultures exposed to virus in the absence of virucidal agent, and are presented as the mean of five independent experiments using explants from separate donors. Detection of proviral DNA by PCR at the right is shown as number of experiments with detectable proviral DNA out of the total tested (limit of detection, 10 copies/explant).

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

HIV infection of genital mucosa is enhanced by immune activation. The observation that immune activation (PHA and IL-2) enhanced HIV infection, using a range of viral isolates (RF, IIIB, 2044, 2076, and SL-2), suggests that transmission and productive infection of genital mucosa are likely to be enhanced by concomitant immune activation in vivo. Thus, infection with STDs resulting in local inflammation, and activation of local inflammatory cells potentially renders an individual more susceptible to HIV infection. Such observations fit with reported epidemiological evidence of a correlation between STDs and HIV infection (10, 19), although this was not observed by others (41). Furthermore, our in vitro studies are supported by recent studies demonstrating an association between STDs and increased CD4 levels and coreceptor expression in genital mucosa (16, 24, 30). Demonstration that these isolates can establish productive HIV infection of cervical explants even if immune activation is delayed up to 8 days after in vitro exposure suggests that immune stimulation caused by venereal infection in vivo potentially facilitates and amplifies localized HIV infection subsequent to the transmission event. It is beyond the scope of this experimental model to determine how long such a reservoir of localized HIV infection may persist; however, the potential ability of venereal infection to activate localized HIV infection in this manner has serious implications for transmission of HIV.

Prime target cells for HIV infection of the female genital tract. The described study has confirmed that subepithelial cells are the prime target cells for HIV infection in female genital tract mucosal tissue in organ culture. Furthermore dual immunohistochemistry identified the majority of HIV-infected cells as subepithelial macrophages. Thus, these cells may represent the main target cells for HIV infection in the female genital tract. There was no evidence of HIV infection of cells within the cervical epithelium. This is in keeping with numerous previous studies demonstrating that following vaginal transmission of either HIV or SIV, infection is exclusively restricted to the subepithelial cells (20, 21, 23, 27, 35). The lack of HIV infection within the epithelium is highly likely to reflect recent observations that expression of CCR5, CCR3, and CXCR4 in cervical tissue is predominantly restricted to subepithelial cells (24, 44). However, it cannot be excluded that cell populations within the epithelium can harbor viral infection below limits of detection by immunohistochemistry.