INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

The RNA-dependent RNA polymerase of the prototypic rhabdovirus, vesicular stomatitis virus (VSV), is comprised of two virally encoded proteins: the large protein, L, and the phosphoprotein, P (1, 18, 20). A complex containing both the L and P proteins carries out transcription and replication of the viral genome (see reference 1 for a review). The template recognized by the RNA polymerase complex is the viral nucleocapsid, which contains the ~11-kb-long negative-sense genomic RNA surrounded by the viral RNA-binding nucleocapsid protein, N. During transcription, the nucleocapsid template is utilized by the RNA polymerase complex to generate subgenomic, capped, and polyadenylated mRNAs, which are translated to produce the viral proteins. During replication, the same RNA polymerase is believed to be switched from transcriptive to replicative mode to synthesize a full-length, positive-sense complementary RNA (or antigenomic RNA) encapsidated by the N protein, which serves as the template for synthesis of progeny nucleocapsids.

Biochemical and mutational studies have suggested that the L protein is multifunctional and carries the catalytic center for polymerization of ribonucleotides during RNA synthesis (50, 51). It presumably also carries other enzymatic activities, including methyltransferase, guanylyltransferase, and poly(A)polymerase activities necessary for generation of mature viral mRNAs (27-29, 32). The multifunctional P protein, on the other hand, is required for the manifestation of the functions of the L protein (1) and plays key roles in both transcription and replication of the viral genome. It interacts specifically with the L protein to form the polymerase complex of the virion (21) as well as stabilizing the L protein against proteolytic degradation (9). It interacts with the terminal sequences of the viral genome (33, 34), presumably for viral RNA synthesis. The P protein also interacts with the N protein to form soluble N-P complexes required for encapsidation of nascent RNA chains during replication (36, 48). Furthermore, P interacts with itself to form oligomers that are required for transcription (15, 23, 24).

The P proteins of both the Indiana (P_I) and New Jersey (P_NJ) serotypes of VSV are highly acidic and consist of 265 and 274 amino acids, respectively. Although these two proteins have only 30% similarity at the primary sequence level, three functionally homologous domains (Fig. 1A) have been defined in mutational studies (25, 47). Domain I, spanning residues from 1 to about 150, is the amino-terminal acidic domain and is phosphorylated. Domain II spans from residues 210 to about 244 and is also phosphorylated. Domain III consists of 21 to 25 residues at the extreme carboxy terminus of the protein and is highly basic. Between domains I and II, a hypervariable region (called the hinge) of approximately 50 to 60 amino acids is present, but its function is unknown.

View larger version (27K): [in this window] [in a new window]   FIG. 1.   (A) Domain structure of the P protein of VSV (Indiana serotype). The P protein with the three functionally defined domains (I, II, and III) and the hinge region is shown. The phosphate acceptor sites (Ser-226, Ser-227, and Ser-233) within domain II are represented by solid circles. (B) Various mutant P proteins with substitutions of alanine (A) or glutamic acid (E) residues in place of serine residues are shown.

In the virion core as well as in infected cells, different phosphorylated forms of the P protein have been shown to exist (6, 13, 30, 31). Furthermore, functional P proteins with different degrees of phosphorylation have been documented (10, 35, 41, 49). It has been shown that the unphosphorylated form of P protein is inactive in transcription in vitro (2, 3). However, it becomes transcriptionally active when first phosphorylated by cellular casein kinase II (CKII) (3, 4, 15). The acceptor sites for CKII-mediated phosphorylation have been mapped to Ser-60, Thr-62, and Ser-64 in domain I of P_I (12, 52) and Ser-59 and Ser-61 of P_NJ (53). Phosphorylation of these residues was recently shown to mediate the multimerization of P protein that facilitates complex formation with the L protein (15, 23, 24). Using a panel of mutant P_I proteins in which the phosphorylation sites in domain I were altered to alanines, we recently showed that phosphorylation of these domain I residues is necessary for the function of the P protein in transcription in vivo (45). The replication functions of the P protein in vivo were unaffected by the phosphorylation status of the domain I residues. Based on these studies, it was proposed that an increase in net negative charge in this region of the protein as a result of phosphorylation is crucial for transcriptional activation of the P protein (45). However, in a separate study, low levels (~25%) of transcription under in vitro conditions were observed with a phosphorylation-defective P mutant protein (52).

Phosphorylation of serine residues in domain II also seems to control the activity of the P protein in RNA synthesis. It was shown that phosphorylation of two specific residues (Ser-236 and Ser-242) in domain II of P_NJ by L-associated kinase (LAK) is necessary for in vitro transcription activity of the P protein (11). For P_NJ, domain I must be phosphorylated prior to LAK-mediated phosphorylation of domain II residues (4). P_I seems to be different in this respect, since domain II residues can be phosphorylated by LAK without prior phosphorylation of domain I residues (12). However, in a separate study, it was shown that substitution of aspartic acid residues at S-60 and T-62 in domain I of P_I results in a protein that is not phosphorylated at other sites (24). Since phosphorylation in domain I is necessary and sufficient for transcription, it appears that phosphorylation of domain II residues may not be involved in transcription (23, 40). In P_I, the Ser-226 and Ser-233 residues are homologous to Ser-236 and Ser-242 residues in P_NJ and were therefore proposed to be the potential sites of phosphorylation (23). However, the actual sites of phosphorylation have recently been mapped to Ser-226 and Ser-227 residues in P_I (12).

To examine the role of phosphorylation of domain II residues in the function of P protein in VSV RNA transcription and replication in vivo, we have used a panel of mutant P proteins with alanine substitutions at the phosphate acceptor sites. Analysis of the mutant P proteins for their ability to support replication of a VSV minigenomic template in vivo showed that phosphorylation of the serine residue at either 226 or 227 is necessary for optimal replication activity of P protein. In vivo-transcription activities of the mutant P proteins were unaffected. Furthermore, substitution of negatively charged glutamic acid residues for these serine residues resulted in a protein that was only 14% as active as the wild-type protein in replication while retaining almost full activity in transcription. These results suggest that phosphorylation of domain II residues is required for optimal replication, but not for transcription, function of the P protein.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Cells and viruses. Growth and maintenance of baby hamster kidney (BHK-21) cells (ATCC CCL10) have been described previously (46). VSV (Indiana serotype; San Juan strain) was grown, and infectivity titers were determined in BHK-21 cells. The recombinant vaccinia virus (vTF7-3) containing the bacteriophage T7 RNA polymerase gene (22) was grown in BHK-21 cells, and infectivity titers of stock vTF7-3 were determined as described earlier (45).

VSV protein- and minigenome-expressing plasmids. The plasmids encoding the wild-type P protein (P_wt) or various mutant P proteins (P₂₂₆, P₂₂₇, P₂₃₃, P_226/227, P_226/233, P_227/233, and P_226/227/233) of VSV (Indiana serotype; Mudd-Summers strain) in pET-3a vector have been described previously (12). These mutant P proteins contain serine-to-alanine amino acid substitutions at positions 226, 227, 233, 226-227, 226-233, 227-233, and 226-227-233, respectively, within domain II of the P protein (Fig. 1). The P_EE mutant contains glutamic acid residues in place of serine residues at positions 226 and 227 of wild-type P protein. Plasmids pN and pL, carrying the coding regions for VSV (Indiana serotype; San Juan strain) proteins N and L, respectively, under the control of the T7 RNA polymerase promoter, have been reported before (46). Construction of the plasmid p9BN, encoding an antigenomic-sense VSV minigenome, has also been described earlier (39).

Virus infections and DNA transfections. The details of the virus infection and DNA transfection procedures have been described in our earlier publications (44, 45). Briefly, BHK-21 cells in 60-mm-diameter plates or in 35-mm-diameter six-well plates were grown to approximately 90% confluency. These cells were infected with vTF7-3 at a multiplicity of infection of 10 PFU per cell. Following virus adsorption at 37°C for 45 min, the cells were washed with serum-free Dulbecco's modified minimal essential medium (DMEM) and transfected with various combinations of plasmid DNAs encoding the VSV proteins and the minireplicon RNA by using Lipofectin reagent (Gibco/BRL, Gaithersburg, Md.) or a transfection reagent prepared in the laboratory as described previously (8). At 4 h posttransfection, the medium was removed from the transfected cells and the cells were washed twice in DMEM supplemented with 2% fetal bovine serum (FBS) and incubated at 37°C with an appropriate volume of the same medium for different lengths of time before metabolic labeling with radioactive precursors. Unless specifically described in the figure legends, in most experiments, 3 µg of pN, 2 µg of pET-3P, 1 µg of pL, and 5 µg of p9BN plasmids were used in transfection of cells in 60-mm-diameter plates. These plasmid amounts were reduced by half for cells in six-well plates.

Metabolic labeling and analysis of proteins. [³⁵S]methionine labeling of proteins in transfected cells, immunoprecipitation, and subsequent sodium dodecyl sulfate-polyacrylamide gel analysis of the proteins have been described previously (45). P proteins were immunoprecipitated by using a rabbit anti-P (Indiana serotype; Mudd-Summers strain) antibody.

Metabolic labeling and analysis of transcription and replication products. Following incubation of transfected cells for 15 to 16 h at 37°C, the cells were pretreated with 15 µg of actinomycin D per ml of DMEM plus FBS at 37°C for 45 min. The cells were then labeled with 15 µCi of [³H]uridine and/or 15 µCi of [³H]adenosine per ml of DMEM plus FBS containing actinomycin D. The labeling of RNAs was performed for 6 to 8 h at 37°C. Cytoplasmic extracts from these cells were prepared in lysis buffer as described earlier (46). The labeled RNA replication products in nucleocapsids were immunoprecipitated by polyclonal anti-VSV antibodies and recovered by extraction with phenol and chloroform and precipitation with ethanol. The recovered RNAs were then separated by electrophoresis in agarose-urea gels (38) and detected by fluorography (37) as described in detail previously (46). For transcription analysis, total RNA from the cytoplasmic extracts was purified by extraction with phenol and chloroform and recovered by precipitation with ethanol. To generate VSV mRNAs, the cells were infected with VSV at a multiplicity of infection of 10 and labeled with [³H]uridine at 2 to 6 h postinfection in the presence of actinomycin D. Total RNA was recovered from the cytoplasmic extracts. The RNAs were analyzed by electrophoresis and fluorography as described above. The quantitation of replication and transcription product RNAs was carried out by densitometric scanning of the fluorograms with an IS-1000 digital imaging system version 2.02 (Alpha Innotech Corporation, San Leandro, Calif.). The readout values from the scanner provide integrated intensities of the appropriate bands in the fluorograms. The relative levels of replication and transcription supported by various mutants were calculated, with the values obtained for the wild-type P protein as 100. For normalization of levels of transcription supported by the mutant P proteins, we used the following formula: normalized levels of transcription = relative levels of transcription/relative levels of replication × 100.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

The phosphorylation of serine residues at positions 236 and 242 within domain II of P_NJ was shown previously to play a major role in the function of P protein in transcription in vitro (11). Based on amino acid sequence homology, corresponding serine residues at positions 227 and 233 of P_I were proposed to be the potential sites for phosphorylation (23). Recently, however, biochemical and mutational studies identified serine residues at positions 226 and 227 to be the sites of phosphorylation (12). To examine the role of phosphorylation of these residues in P protein function in transcription and replication, we used a series of mutant P proteins (Fig. 1B) in which serine residues at positions 226, 227, and 233 were altered to alanines either individually (in mutants P₂₂₆, P₂₂₇, and P₂₃₃) or in various combinations (in mutants P_226/227, P_226/233, P_227/233, and P_226/227/233). In initial experiments, we found that in transfected cells, the mutant P proteins were expressed at levels similar to that of the wild-type P protein and the amount of P protein expression was roughly proportional to the amount of plasmid transfected into the cells within a range of 1 to 15 µg. Furthermore, in pulse-chase experiments, the mutant proteins were stable over an 8-h chase period (data not shown).

In our earlier studies, we showed that the wild-type P protein of the Mudd-Summers strain of VSV functions as well as the San Juan strain of VSV P protein in transcription and replication with San Juan N and L proteins (45); therefore, the above-mentioned mutant P proteins of the Mudd-Summers strain were used in order to test their abilities to support transcription and replication in the presence of the N and L proteins of San Juan VSV.

Phosphorylation-defective mutant P proteins do not support optimal replication of VSV. To examine the ability of the mutant P proteins to support replication of a VSV minigenome, we used a plasmid encoding an antigenomic-sense VSV minireplicon (9BN) RNA (39). The minireplicon RNA contains the terminal sequences of the VSV antigenome flanking the sequences of the N gene and part of the L gene (39). In cells transfected with the plasmid along with the plasmids encoding the viral proteins N, P, and L, the antigenomic-sense minireplicon RNA is replicated and amplified to generate the genomic- and antigenomic-sense RNAs (39). However, the genomic-sense RNA replication products can be more readily detected by metabolic labeling of the transfected cells, since the genomic RNAs are synthesized in much greater molar abundance than the antigenomic-sense RNAs. To perform the experiment, BHK-21 cells infected with vTF7-3 were transfected with plasmids encoding the minireplicon and N, L, and the wild-type or mutant P proteins. [³H]uridine- and [³H]adenosine-labeled RNAs synthesized in the presence of actinomycin D were recovered from immunoprecipitated nucleocapsids and analyzed by electrophoresis. The results (Fig. 2) from a typical experiment show that the mutant P proteins P₂₂₆, P₂₂₇, P₂₃₃, P_226/233, and P_227/233 (Fig. 2, lanes 4 to 6, 8, and 9) were as active as the wild-type P protein (Fig. 2, lane 3) in replication, since the amounts of replication product obtained with the mutant proteins were comparable to that of the wild-type P protein. However, the replication activities of the mutant proteins P_226/227 (Fig. 2, lane 7) and P_226/227/233 (lane 10) were significantly less than that of the wild-type P protein. In three to five independent experiments, the average replication activity of P_226/227 and P_226/227/233 mutants was found to be 8 and 13%, respectively (Table 1; also see Fig. 5) of that of the wild-type protein.

View larger version (58K): [in this window] [in a new window]   FIG. 2.   Replication of a VSV antigenomic minireplicon with P mutants. BHK-21 cells in 60-mm-diameter plates were infected with vTF7-3 and transfected with plasmids encoding the minigenomic RNA and the N, L, and wild-type or mutant P proteins. At 16 h posttransfection, the cells were labeled with [3H]uridine and [3H]adenosine in the presence of actinomycin D for 6 h. The replicated RNAs were recovered by immunoprecipitation and analyzed by electrophoresis. The arrow indicates the negative-sense replication product. The asterisk shows the transcription product which is sometimes nonspecifically immunoprecipitated by anti-VSV antibodies. G and N at left represent the G mRNA and N mRNA of VSV isolated from infected cells labeled with [3H]uridine.

View larger version (44K): [in this window] [in a new window]   FIG. 3.   Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immunoprecipitated wild-type (A) and P226/227 (B) proteins expressed in cells transfected with various amounts (indicated in micrograms above each lane) of the plasmids. The expressed proteins are shown by the arrows in these panels. (C) Replication of VSV minireplicon RNA in the presence of various amounts (indicated in micrograms above each lane) of transfected P226/227 plasmid. Analysis of replication was performed as described in the legend to Fig. 2. The arrow indicates the negative-sense replication product. The asterisk shows the transcription product which is sometimes nonspecifically immunoprecipitated by anti-VSV antibodies.

Transcription activity of the mutant P proteins remains unaffected. We next examined the ability of these mutant proteins to support transcription in vivo. BHK-21 cells infected with vTF7-3 were transfected with plasmids encoding 9BN minireplicon RNA and N, L, and wild-type or mutant P proteins. The transfected cells were then labeled with [³H]uridine and [³H]adenosine in the presence of actinomycin D. Total labeled RNAs isolated from these cells were analyzed by electrophoresis. Synthesis of NL mRNA (Fig. 4) was used as a measure of the transcription activity of the mutant P proteins. The results (Fig. 4) show that P₂₂₆, P₂₂₇, P₂₃₃, P_226/233, and P_227/233 (lanes 4 to 6, 8, and 9) are almost as active as the wild-type P protein (lane 3) in transcription. However, the amount of NL mRNA synthesized by the P_226/227 (lane 7) and P_226/227/233 (lane 10) mutants was significantly reduced. A quantitative determination of the levels of transcription suggests that the P_226/227 and P_226/227/233 mutants were 8 and 12%, respectively, as active as the wild-type protein (Table 1).

View larger version (62K): [in this window] [in a new window]   FIG. 4.   Transcription activity of P mutants. BHK-21 cells in 35-mm-diameter six-well plates were infected with vTF7-3 and then transfected with 1.5 µg of pN, 0.5 µg of pL, 2.5 µg of p9BN, and 1.0 µg of pET-P plasmids coding for the wild-type or mutant P proteins. The transfected cells were labeled with [3H]uridine and [3H]adenosine at 16 h posttransfection for 6 h in the presence of actinomycin D. Total RNA from the cytoplasmic extracts was recovered and analyzed by electrophoresis as described in Materials and Methods. G and N represent the corresponding VSV mRNAs isolated from infected cells. The asterisk shows the NL RNA transcription product.

View larger version (42K): [in this window] [in a new window]   FIG. 5.   Relative levels of replication and normalized levels of transcription supported by various mutant P proteins. The data were obtained by densitometric scanning of fluorograms as described in Materials and Methods. The histograms represent data from four to five separate experiments, including those shown in Fig. 2, 4, and 6. The average and range of each value are shown.

Substitution of glutamic acid at the phosphate acceptor sites does not rescue optimal replication functions of P protein. One of the consequences of phosphorylation of amino acid residues in a protein is the introduction of negative charges. We wondered whether phosphorylation per se of the Ser-226 and Ser-227 residues of P protein is essential for optimal replication functions of the protein or whether substitution of negatively charged residues in place of these serine residues can generate replicationally active P protein. To address this possibility, we generated a mutant P protein (P_EE) in which serine residues at positions 226 and 227 were replaced with glutamic acid residues. The ability of the mutant protein to support replication of the VSV minigenome was analyzed. The results (Fig. 6A) show that the P_EE mutant (lane 5) is slightly more active in replication than the analine-substituted mutant protein (lane 4). However, the level of replication supported by P_EE is only 12 to 14% of that of the wild-type protein (lane 3). When the transcription activity of these mutant proteins was measured, P_EE consistently produced slightly higher levels of NL mRNA (Fig. 6B, lane 5) than P_226/227 (lane 4). This is most likely due to higher levels of transcription templates generated by the P_EE mutant than the P_226/227 mutant. When the level of transcription was normalized to the amount of transcription template, P_EE was found to be about 90 to 95% as active as the wild-type P protein (Fig. 5).

View larger version (52K): [in this window] [in a new window]   FIG. 6.   Replication (A) and transcription (B) supported by PEE. These experiments were performed as described in the legend to Fig. 2 and 4. The arrow in panel A and the asterisk in panel B show the replication and transcription products, respectively.

P_226/227 mutant protein acts as an inhibitor of wild-type P protein in replication. We next wanted to determine whether the P_226/227 mutant, which is defective in supporting optimal levels of replication, when coexpressed along with the wild-type protein, can interfere with the normal functioning of the wild-type protein in replication. To examine this, replication of the VSV minigenome in transfected cells coexpressing various amounts of P_wt and P_226/227 proteins was analyzed. The results from such an experiment (Fig. 7) show that coexpression of a fixed amount of P_wt and varying amounts of P_226/227 (lanes 6 to 8) led to significant inhibition of minigenome replication. It must be noted that increasing the amounts of P_wt or P_226/227 led to inhibition of minigenome replication (data not shown), most likely due to imbalance in the molar amounts of the viral proteins required for replication (46). However, the inhibitory activity of P_226/227 is clearly evident when one compares levels of replication supported by a given amount of P_wt with that supported by the same amount of both P_wt and P_226/227. The amount of replication with 4 µg of total plasmids (2 µg each of P_wt and P_226/227) (Fig. 7, lane 7) was approximately one-third of that obtained with 4 µg of P_wt plasmid alone (lane 3). Likewise, the amount of replication with 6 µg of total plasmids (2 µg of P_wt and 4 µg of P_226/227) (Fig. 7, lane 8) was approximately one-fourth of that obtained with 6 µg of P_wt plasmid alone (lane 4). In addition, when 2 µg of P_wt plasmid was used along with increasing amounts of P_226/227, significant inhibition of replication was observed (compare the levels of replication in Fig. 7, lane 2, with those in lanes 6 to 8). These results, therefore, indicate that P_226/227 protein inhibits the function of P_wt protein in replication in vivo.

View larger version (42K): [in this window] [in a new window]   FIG. 7.   Inhibitory activity of P226/227 in replication when coexpressed with wild-type P. The value above each lane indicates the amount (in micrograms) of each P plasmid used in the transfection. The experiment was performed as described in the legend to Fig. 2. The arrow shows the replication product.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

The phosphoprotein (P) of VSV plays a central role in the transcription and replication functions of the RNA-dependent RNA polymerase of the virion. Although the P protein does not carry any enzymatic activities of the viral polymerase, its interaction with the large protein, L, generates a specific P-L complex(es) which is absolutely essential for the polymerase activity. The P protein interacts with soluble N protein to form N-P complexes that are required for encapsidation of nascent RNA chains during replication. P protein is known to exist as oligomers, and oligomerization of P protein appears to be required for interaction with the L protein to generate transcription activity of the P-L complex. In addition, the P protein exists in multiple phosphorylated forms within the virion core as well as in infected cells, and phosphorylation of residues within specific domains of the P protein regulates its RNA-synthetic activities. Because of the multifunctional nature of the P protein, a great deal of investigation has been directed towards understanding its structure-function relationships.

We and others have shown recently that phosphorylation of specific residues within domain I of P protein of Indiana serotype VSV is not essential for the in vivo replication function of the protein (45, 52). However, in vivo transcription activity of the protein required phosphorylation of the residues in this domain (45), although low levels of in vitro transcription activity were obtained with domain I phosphorylation-defective P protein (52). In the present work, we have examined the role of phosphorylation of domain II residues in in vivo transcription and replication activities of the protein. Our results suggest that phosphorylation of a specific serine residue(s) (Ser-226 or Ser-227) in this domain is required for optimal replication activity of the protein. Interestingly, transcription activity of the protein was not adversely affected by the phosphorylation status of the domain II residues. The major conclusion that can be drawn from our studies is that phosphorylation at two different domains in the P protein of VSV regulates its activity in transcription and replication. However, it must be pointed out that the requirement for phosphorylation of domain I residues in transcription could be overcome by substitution of negatively charged residues for serine residues (24, 45) whereas appreciable levels of replication could not be rescued by substitution of glutamic acid residues for serine residues in domain II (Fig. 6). These results may suggest a unique role of phosphorylation in the function of the P protein in replication but not in transcription.

Recently, it was demonstrated (23) that serine residues at positions 227 and 233 within domain II of P_I are the major targets of phosphorylation by LAK. However, in a separate study, it was shown by mutational and biochemical analyses that serine residues at 226 and 227 are the sites of phosphorylation by LAK (12). The reason(s) for this discrepancy is not clear at this time but the results of our functional analysis of the mutant P proteins, in which each of these three sites was altered to alanine individually or in various combinations, indicate that Ser-233 does not appear to play any role in transcription or replication functions of the protein. Our results are consistent with those reported earlier from in vitro-transcription studies, suggesting that phosphorylation of domain II residues by LAK has no role in the transcription activity of P_I (23, 40). These results appear to conflict with those obtained previously, which showed that phosphorylation of Ser-236 and Ser-242 in domain II of P_NJ by LAK is necessary for efficient transcription in vitro (11). It is possible that the activity of the mutant proteins differs under in vivo and in vitro conditions, as has been demonstrated recently with domain I phosphorylation-defective P mutant proteins (45, 52). Alternatively, it is possible that the functional requirement for phosphorylation of domain II residues of P_NJ may be different from that of P_I. This may be borne out by the observation that phosphorylation of domain I residues of P_NJ is a prerequisite for phosphorylation of domain II residues (4) whereas domain II of P_I can be phosphorylated without prior phosphorylation of domain I residues (12).

The observation that domain II phosphorylation-defective P protein is defective in replication but not in transcription indicates that phosphorylation of domain II residues may be important for formation of replicase complexes. We recently suggested that the replicase complex of VSV may exist as a tripartite complex consisting of the N, P, and L proteins (16) and that other cellular factors may also be present in the replicase complex (26). It is possible that phosphorylation of domain II residues results in a conformation that facilitates specific interaction with the L and/or N protein for assembly of the replicase complex. The P protein, lacking phosphates in domain II residues, may interact poorly with the other viral proteins so that low levels of replication complexes are formed or that the replication complexes are less active. It is obviously important to examine the interaction of the mutant P proteins with the N and L proteins to understand how phosphorylation in domain II of P might influence the assembly and activity of the replicase complex. It must be noted here that the mutant protein (P_227/233) multimerized and interacted well with the L protein (23). However, the data should be considered in the context that this mutant protein was phosphorylated at Ser-226 (12).

Several recent studies have provided a correlation between the degree of phosphorylation of the P protein and its activity in transcription and replication. Using okadaic acid, a serine/threonine phosphatase inhibitor, it was shown that the hyperphosphorylated form of the P protein (presumably having phosphates in both domain I and II residues) inhibited replication (10). Conversely, dephosphorylation of P protein by bacterial alkaline phosphatase (BAP) led to increased genome replication, with a concomitant decrease in transcription (49). The amount of BAP needed to stimulate genome replication and inhibit transcription was substantially below that needed to obtain a noticeable increase in reactivity to a monoclonal antibody (6D11) directed against an epitope that maps to residues 177 to 227 of the P protein (49, 55), corresponding to part of domain II. Although these studies did not map the sites from which phosphate moieties were removed by BAP treatment, they indicated that the epitope bound by 6D11 antibody is changed by dephosphorylation (49). Since treatment of infected-cell P protein with BAP increased genome replication and inhibited transcription without a noticeable increase in 6D11 antibody reactivity, these results indicate that BAP may have removed phosphates only from domain I residues. These observations, together with our previous results (45) and the data presented here, suggest that the P1 protein, with phosphorylation of domain I residues, may function as part of a transcription complex whereas the P2 protein, with phosphorylation of residues in domain II, may function exclusively as part of a replication complex. Another subset of P proteins (hyperphosphorylated P2 protein with phosphorylation of both domain I and II residues) may be involved in maturation of nucleocapsids for packaging and thereby indirectly inhibit replication (10). Further studies focusing on isolation of complexes specific for transcription and replication and examination of the phosphorylation status of P protein in these complexes may provide more direct evidence as to the role of phosphorylation of residues in various domains in transcription and replication.

The inhibitory activity of P_226/227 in replication by wild-type P protein (Fig. 7) indicates that the domain II phosphorylation-defective P protein perhaps interacts with the other components of the replication machinery to generate inactive complexes or competes with the wild-type P protein for replication machinery. Studies to examine specific interactions of the mutant protein with the N, L, or N-RNA template may provide a better understanding of the inhibitory effect of the mutant protein in replication.

The phosphate acceptor sites (Ser-226 and Ser-227) within domain II are located adjacent to each other, and our results (Fig. 2) show that phosphorylation of either of these two residues is sufficient to obtain optimal replication activity of the protein. Furthermore, previous data indicate that phosphorylations at these sites are independent of each other (12). The question then is why two functionally redundant phosphorylation sites are maintained in the protein. It is possible that in a given P molecule, only one of these two sites is phosphorylated in vivo and the other site may remain unphosphorylated because of steric hindrance. Alternatively, the presence of two functionally redundant phosphorylation sites next to each other may protect the protein from becoming inactivated in replication by phosphatases, which must remove both of the phosphate moieties at the same time.

In summary, the results reported in this article suggest that phosphorylation of either the Ser-226 or Ser-227 residue in domain II is necessary for optimal replication activity, but not for transcription activity, of the P protein. Substitution of negatively charged residues for these residues cannot rescue replication. From these results and those reported previously (10, 23, 24, 45, 49, 52), we conclude that phosphorylation within different domains of the P protein controls its activity in transcription and replication. Studies of infectious molecular clones of VSV containing various phosphorylation-defective mutants of P protein may provide additional information as to the role of this modification in the RNA-synthetic processes of VSV. In several other negative-strand RNA viruses, the P protein has been found to be phosphorylated (5, 7, 14, 17, 19, 42, 43, 54), and the functional significance of phosphorylation in transcription has also been documented (5, 7, 17, 42). Whether transcriptase and replicase activities are regulated by differential phosphorylation of the P proteins of these viruses remains to be examined.