INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

Bovine papillomavirus type 1 (BPV-1) has been studied extensively as a model for papillomavirus replication and transcription. The viral E2 protein is the master regulator of the viral life cyclethis protein modulates the transcription of viral genes (41) and is responsible for the initiation of DNA replication (43, 44, 48) and for the stable maintenance of the viral genome (31), which is achieved presumably through facilitation of the association of the viral genome with chromatin (19a, 23, 40). E2 is a sequence-specific DNA-binding protein, and it interacts with the components of the cellular transcription (33, 49) and replication (24) machinery. The viral E2 and E1 proteins interact with each other (2, 4, 30, 35) during the initiation of replication, resulting in cooperative binding of E1 and E2 on the BPV-1 replication origin (25-27, 35-39).

The BPV-1 E2 protein, like other transcription factors, is composed of relatively well-defined function-specific modules. Structural and mutational analyses have revealed three distinct domains. The amino-terminal part (residues 1 to 210) is an activation domain for transcription (12, 28) and replication (43). It is followed by the unstructured hinge region and the carboxy-terminal DNA-binding and dimerization domain (residues 310 to 410) (29). Deletion analysis of the E2 protein has shown that the transactivation domain and the DNA-binding and dimerization domains are necessary for both replication and transcription, while large deletions in the hinge region affect replication preferentially and transcription less (46). The structure of the carboxy-terminal DNA-binding and dimerization domain has been solved by X-ray analysis and has revealed a dimeric DNA-binding and dimerization motif (15, 16). Most of the information about structural and functional determinants in the amino-terminal activation domain of the E2 protein has been obtained by mutational analysis (7, 12, 14, 46). These data confirm that the E2 amino-terminal domain, like the C-terminal domain, has a highly organized structure and that even a single point mutation can inactivate the function of the E2 protein in the activation of transcription, replication, or both (1, 5, 9, 13, 34).

Antibodies are efficient and highly specific tools for identifying the structural determinants of macromolecules and/or for studying the role of a protein in functional assays (18, 19, 21, 42, 45). Antibodies have been used for the characterization of the human papillomavirus (HPV) E2 protein. For example, polyclonal antibodies against overlapping synthetic peptides that cover the HPV type 16 (HPV-16) E2 protein have been used to test the structure of this protein (10), and the interaction of the HPV-16 E2 protein with the E1 protein could be blocked by a monoclonal antibody (MAb) that bound E2 in the region of amino acids 18 to 41 (17).

In this study, we describe the production of a set of MAbs against the BPV-1 E2 protein and characterize their ability to interfere with the functions of the E2 protein in vivo and in vitro in biochemical and functional assays.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Production of the BPV-1 E2 protein. E2 protein was expressed in the pET11c-based system in Escherichia coli and was purified to homogeneity by conventional methods (37) with modifications. First, we precipitated nucleic acids from clarified cell lysates by the slow addition of polyethylenimine (Polymin P; Sigma) to a final concentration of 0.6%. Precipitation was carried out on ice for 30 min, and the pellet was collected by centrifugation. Proteins were recovered from the supernatant by precipitation with 35% ammonium sulfate and purified to homogeneity by conventional chromatography.

Production of MAbs. Female BALB/C mice were injected with 50 µg of purified BPV-1 E2 protein five times at 3- to 4-week intervals. The injections were intraperitoneal, with E2 suspended initially in Freund's complete adjuvant and subsequently in phosphate-buffered saline (PBS). Following the final injection, mice were allowed to rest for 5 weeks and then were injected with 100 µg of antigen. One week later, final boosts with 100, 200, and 200 µg of protein in PBS at 4, 3, and 2 days before fusion, respectively, were performed. Sp2/0 myeloma cells and cells from one third of the spleen were washed three times with sterile PBS. The final pellet was mixed by tapping the tube, and 1 ml of 50% polyethylene glycol (PEG) 4000 (Merck) was added over 1 min with gentle shaking. The cells were centrifuged at 100 × g for 5 min, the PEG solution was removed, and the resuspended cells were plated on five 96-well microtiter plates containing hypoxanthine-aminopterin-thymidine medium. Supernatants were tested 10 days after fusion as described below by a direct enzyme-linked immunosorbent assay (ELISA).

Screening of hybridomas. Wells of ELISA plates (Maxisorp; Nunc, Roskilde, Denmark) were coated with 100 µl of E2 (2 µg/ml in PBS) overnight at 4°C. After the coating step, the plates were washed three times with PBS containing 0.05% Tween 20 (PBS/T) and blocked with PBS/T containing 0.05% casein for 30 min. Then, 80 µl of PBS/T and 20 µl of hybridoma supernatants were added to the wells, and the plates were incubated for 60 min on a shaker at room temperature. The plates were washed with PBS/T, followed by the addition of 100 µl of peroxidase-conjugated goat anti-mouse immunoglobulin G (IgG) (LabAS Ltd., Tartu, Estonia) diluted 1:2,500 in PBS/T supplemented with 2% PEG 6000 (Merck). The plates were incubated for 15 min and then washed with PBS/T. Then, 100 µl of TMB substrate solution (3,3',5,5'-tetramethylbenzidine-H₂O₂ in 0.1 M acetate-citrate buffer [pH 4.5]) was added.

Purification of MAbs. The MAbs were purified from ascitic fluid by ammonium sulfate precipitation and ion-exchange chromatography on Blue DEAE-Toyopearl 650S with a Pharmacia standard fast protein liquid chromatography system (20). The IgG concentration was estimated at 280 nm by use of an extinction coefficient of 14. The purified MAbs were stored in PBS containing 50% glycerol at 20°C.

Preparation of Fab' fragments. Fab' fragments were prepared from the MAbs as described by Porter (32) with modifications. The MAbs were dialyzed against 0.1 M sodium acetate buffer (pH 5.5) containing 1 mM EDTA and 25 mM 2-mercaptoethanol. The antibodies were digested with papain at an enzyme/antibody ratio of 1:10 (wt/wt) for 24 h at 37°C. The reaction was stopped by the addition of iodoacetamide to a final concentration of 30 mM. The digested antibodies were dialyzed against 20 mM Tris-HCl buffer (pH 7.2), and Fab' fragments were purified on a Mono Q column.

Peptide synthesis. Peptides were assembled in a stepwise manner on a solid support with a model 431A peptide synthesizer (Applied Biosystems) by the standard NMP/HOBt solvent activation strategy on a 0.1-mmol scale (22).

ELISA with peptides. The surfaces of the microtiter wells were activated with 0.25% glutaraldehyde in PBS for 30 min at 60°C. The plates were washed three times with PBS, and a peptide solution at a concentration of 20 µg/ml in PBS was added to the wells. The plates were incubated overnight at room temperature, washed with PBS/T, and blocked with 1% nonfat dry milk in PBS/T for 2 h. The plates were washed with PBS/T, and antibodies diluted in PBS/T were added to the wells, incubated, and processed as described above.

Plasmids. The pET-E2 vector used for the expression of E2 in E. coli was generated by PCR amplification with specific primers and was cloned between the NdeI and BamHI sites of plasmid pET11c. The E2 expression constructs pCGE2, pCGE2C, pCGE8/E2, and pCGE2(D92-161) have been described previously (43, 44). Plasmid VP16:E2 (24) contains 80 C-terminal amino acids from VP16 fused to the C terminus of E2 (starting from amino acid 250) in the context of pCG. The E2 N-terminal deletion mutants E2(D1-23), E2(D1-85), E2(D1-112), and E2(D1-183) were generated by PCR with appropriate oligonucleotide primers containing an initiation methionine codon in the optimal Kozak context and were cloned into the pCGE2 expression vector at the XbaI-BamHI sites. For the E2 C-terminal deletion mutants E2(D219-410), E2(D284-410), and E2(D310-410), PCR primer pairs were designed with terminal recognition sequences for KpnI-BclI and were cloned into the corresponding sites in pCGE2. The replication reporter plasmid pUCAlu has been described previously (43). pHookAlu was made by cloning the Alu fragment from pUCAlu at the HindIII-BamHI sites of pHook-2 (Invitrogen) and deleting the cytomegalovirus promoter with restriction enzymes HindIII and BssHI.

Cells and transfections. E2 expression constructs (100 ng) were electroporated at 180 V into COS-7 cells (2 × 10⁶ to 6 × 10⁶) in 250 µl of Dulbecco modified Eagle medium supplemented with 10% fetal calf serum (FCS) and 50 µg of denatured salmon sperm DNA at room temperature (43). For replication assays, CHO4.15 cells were trypsinized, centrifuged, and resuspended in F12 medium containing 10% FCS at a density of 10⁷ cells/ml. The cell suspension (250 µl) was mixed with 100 ng of pUCAlu DNA, 50 µg of salmon sperm DNA, and various concentrations of MAbs or Fab' fragments in a disposable electroporation cuvette and subjected to an electric discharge of 230 V from an Invitrogen Gene Pulser. After the discharge, the cell suspension was left at room temperature for 15 min, and then the cells were washed and plated in F12 medium supplemented with 10% FCS. The extraction of episomal DNA from cells and its analysis by Southern blotting were performed as described previously (43). For Western blot analysis, 500 ng of pHookAlu was cotransfected with MAbs (80 µg/ml), and transiently transfected cells were separated from the total population of CHO4.15 cells with magnetic beads (Invitrogen) according to the manufacturer's recommendations.

Immunoblotting of E2. COS-7 cells transfected with E2 proteins in 60-mm diameter dishes were lysed 36 h after electroporation in 200 µl of Laemmli sample buffer. Transfected CHO4.15 cells were separated from the magnetic beads by boiling in 100 µl of Laemmli sample buffer. Proteins were separated by sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (PAGE). After transfer, the nitrocellulose membranes were incubated with mouse E2-specific MAbs and a secondary horseradish peroxidase-conjugated antibody by use of an ECL detection kit (Amersham) according to the manufacturer's recommendations. To analyze the E2 protein level in CHO4.15 cells transfected with MAbs, rabbit anti-E2 polyclonal antibody was used.

Mobility shift assays. For preparation of COS-7 cell extracts, transfected cells were removed from semiconfluent growth on 100-mm-diameter plates with a rubber policeman, washed, and lysed in 100 µl of lysis buffer (20 mM Tris-HCl [pH 8.0], 100 mM NaCl, 30 mM KCl, 0.1 mM EDTA, 0.35% Nonidet P-40, 10 mM dithiothreitol, protease inhibitors) on ice for 30 min. Cell debris was removed by centrifugation, glycerol was added to a final concentration of 20%, and the extracts were divided into aliquots and stored at 70°C. For gel shift assays, 2 µl of cell extract was incubated in 10 µl of binding buffer (10 mM Tris-HCl [pH 7.5], 100 mM KCl, 2 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride, 15% glycerol, 5 mg of bovine serum albumin per ml, 1 µg of leupeptin per ml, 1 µg of aprotinin per ml) at room temperature for 15 min in the presence of 1 µg of sonicated salmon sperm DNA and 0.2 ng of ³²P-labelled oligonucleotide containing the E2 binding site. For bacterially expressed E2, 2 ng of protein was used per reaction. Double-stranded high-affinity binding site 9 of BPV-1 (5'-ACAAAGTACCGTTGCCGGTCGAA-3') was used as a probe. Protein-DNA complexes were resolved by 6% PAGE (acrylamide-N,N-methylene-bisacrylamide, 80:1) with 0.25× Tris-borate-EDTA. Gels were dried and exposed to X-ray film. MAbs (1 to 10 ng/µl) were added either before or after DNA binding and were incubated for an additional 20 min. For protease digestion experiments, bacterially expressed E2 protein was incubated with 2 µg of pronase for 5 min. The E1-E2-origin complex formation assay was performed as described by Sedman and Stenlund (35). The effect of antibodies on E1-E2-origin complex formation was tested either before or after the assembly of the complex.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Generation of E2-specific MAbs. The soluble E2 protein was purified to apparent homogeneity from lysates of isopropyl--D-thiogalactopyranoside (IPTG)-induced E. coli overexpressing BPV-1 E2 from the pET11c expression vector by conventional chromatography (37). BALB/c mice were immunized with the purified functionally active E2 protein as described in Materials and Methods. We obtained nearly 200 hybridoma cell lines, 17 of which were positive for the E2 protein in both ELISAs and immunoblot assays, while 5 hybridomas were positive in ELISAs only. Nine MAbs that were deemed most useful were purified from the ascitic fluids of the respective hybridoma cell lines and studied in various assays as described below (Table 1). All studied antibodies belonged to the IgG1 subtype, with the exception of 3C1, which belonged to the IgG2a subtype. All of these MAbs had high affinities for and fast kinetics of binding to their respective epitopes, as found by the concentration dependence of antibody binding in ELISAs (data not shown).

Epitope mapping. To define the continuous epitopes recognized by the antibodies, the reactivity of each MAb to full-length and truncated E2 proteins expressed in COS-7 cells was determined by Western blot analysis. The linear epitopes for the initially isolated 17 MAbs were mapped in the region between amino acids 184 and 309; for 12, the epitopes were found within the region between residues 184 and 218 (data not shown). We concluded from these results that the sequence of the 34 amino acids within the region from residues 184 to 218 is the major immunodominant determinant of the BPV-1 E2 protein. The results of immunoblot analysis for five selected purified antibodies with the linear epitopes are shown in Fig. 1A. To map the epitopes for the 1E2, 3F12, and 1H10 antibodies more precisely, we synthesized four overlapping peptides covering the region between amino acids 162 and 210. The sequences of the synthesized peptides and the ability of the MAbs to bind to these peptides in an ELISA are shown in Fig. 1B. Peptides P2 (residues 171 to 192) and P3 (residues 184 to 201) were recognized by 1E2, while peptide P4 was recognized by 3F12. To narrow down the sizes of the epitopes, two additional peptides, P5 (residues 179 to 190) and P6 (residues 197 to 208), were synthesized and confirmed by an ELISA to contain the recognition sequences for the 1E2 and 3F12 antibodies, respectively. 1H10 did not recognize any of the synthesized peptides and was therefore mapped by the Western blot analysis to the region between amino acids 208 and 218. 

View larger version (26K): [in this window] [in a new window]   FIG. 1.   Epitope mapping of E2-specific MAbs. (A) Schematic representation of the truncated E2 proteins used and the results of the immunoblot analysis of the E2 proteins (diagram at right). wt, wild type; conf., MAbs with discontinuous epitopes. (B) Reactivity of MAbs to synthetic peptides (P1 to P6) covering the region from amino acids 162 to 210 of E2 in an ELISA. +, positive result; , negative result.

View larger version (30K): [in this window] [in a new window]   FIG. 2.   Characterization of E2-specific MAbs. (A) Reactivity of E2-specific MAbs with the native E2 protein. The mobility shift assay was carried out with 2 ng of bacterially expressed and purified E2 protein and 0.2 ng of radiolabelled E2 binding site for 15 min at room temperature. (B) Lanes 1 to 10 show reactivity of MAbs with discontinuous epitopes with truncated E2 proteins expressed in COS-7 cells. Band shift assays were performed with 2 µl of cell extract. Lanes 11 to 16 show reactivity of MAbs to the E2 DNA-binding domain (DBD). Bacterially expressed E2 protein was treated with 2 µg of pronase for 10 min at room temperature, and then reactivity was determined. (C) Reactivity of MAb 1E2 with truncated E2 proteins expressed in COS-7 cells. MAbs were added after E2 was mixed with its DNA target. neg., cells transfected with carrior only; wt, wild type. Protein-DNA complexes were resolved by 6% PAGE with 0.25× Tris-borate-EDTA.

Accessibility of the MAb 1E2 epitope in the E2 protein. The epitope for MAb 3F12 (residues 199 to 206) is efficiently exposed in the DNA-bound E2 protein, while the epitope for MAb 1E2, located 8 residues upstream, is poorly recognized by the respective antibody in the full-length E2 protein in complex with DNA (Fig. 2A, compare lanes 2 and 3 with lanes 4 and 5). However, the epitope for MAb 1E2 was readily exposed in truncated E2 proteins bound to DNAE2(D1-23), E2(D1-85), E2(D1-112), E2(D1-183), and E2C (Fig. 2C)as well as in the internal deletion mutant E2(D92-161) (data not shown). All of these deletion mutants were inactive for the activation of transcription and replication (21a). Therefore, the epitope for MAb 1E2 could be identified as a transactivation domain denaturation-specific epitope of the E2 protein; the exposure of the 1E2 epitope indicates that the E2 protein transactivation domain has an inactive conformation for transcription and replication.

Antibodies against the C-terminal domain of the E2 protein. MAbs 3E8, 3H5, 5F10, and 5H4 did not react with the E2 protein on immunoblots, indicating that the epitopes of these MAbs are sensitive to denaturation. To define further the epitopes for these MAbs, the reactivity of each MAb to the full-length or truncated E2 protein expressed in COS-7 cells was determined by a gel shift assay. All four studied antibodies were able to react with both the full-length E2 protein (data not shown) and E2C expressed in COS-7 cells (Fig. 2B, lanes 1 to 5). MAbs 3E8, 3H5, and 5F10 were able to induce a supershift, and MAb 5H4 prevented the formation of the E2-DNA or E2C-DNA complex (Fig. 2B, lane 5; see also Fig. 5A, lanes 2 to 5). MAb 5H4 not only prevented the formation of the E2-DNA complex but also dissociated the preformed E2-DNA complex, and this effect was dependent on the concentration of the antibody. The dissociation of the preformed complex required concentrations of MAb 5H4 higher than those required to block complex formation. The chimeric protein VP16-E2, which contains amino acids 250 to 410 of the E2 protein, was recognized by MAbs 3E8, 3H5, and 5H4 but poorly, if at all, by MAb 5F10 (Fig. 2B, lanes 6 to 10).

Effect of MAbs on E1-E2-origin complex formation. The BPV-1 minimal origin of replication comprises the E1 binding site, the A/T-rich region, and the E2 binding site (44). The E1 and E2 proteins bind cooperatively to the origin and form an E1-E2-origin complex (27, 35, 39). It has been shown that the ability of the E2 protein to form a complex with the E1 protein on DNA correlates with the efficiency of initiation of replication in vivo (11, 36). We studied the effect of the antibodies on E2-dependent E1-E2-origin complex formation. Antibodies 3F12, 1H10, 1E4, 3C1, 3E8, 3H5, and 5F10 all recognized their respective E2 epitopes in the E1-E2-origin complex and supershifted this complex (Fig. 3A, lanes 3 to 9). Interestingly, MAbs which recognize the C-terminal part of the E2 molecule resulted in an E1-E2-origin MAb complex which migrated much more slowly than complexes in which the MAbs bound to epitopes closer to the center of the E2 molecule. The epitope for MAb 1E2 is masked in the DNA-bound E2 protein and remains nonaccessible to this antibody in the E1-E2-DNA complex (Fig. 3A, lane 2). MAb 5H4, which specifically dissociated E2 from DNA, prevented the formation of the E1-E2-origin complex (Fig. 3A, lane 10). None of the antibodies had any effect on the mobility of the E1-origin complex formed at a high E1 protein concentration (Fig. 3B). These results showed that the epitopes for the most studied MAbs, with the exception of 1E2, are exposed in the E1-E2-origin complex and that MAb 5H4 is the only antibody which interferes with E2-dependent E1-E2-origin complex formation.

View larger version (53K): [in this window] [in a new window]   FIG. 3.   Effect of E2-specific MAbs on the formation of the E1-E2-origin (ori) complex. (A) A gel mobility shift assay was performed with 2 ng of E1 protein and 5 ng of E2 protein for 20 min. MAbs were added to a final concentration of 10 ng/µl and incubated for an additional 20 min. (B) A gel mobility shift assay was used to analyze the complex formed in the presence of E1 only. The resulting complexes were treated with 0.4% glutaraldehyde and separated on agarose gels.

Effect of anti-E2 MAbs on BPV-1 origin replication in cells. The observation that antibodies recognized their respective epitopes in the DNA-bound E2 protein raised the possibility that some of these antibodies could interfere with some functions of the E2 protein in the initiation of DNA replication in vivo. Therefore, the purified antibodies were tested in a transient replication assay in vivo for their ability to block E2 protein functions in BPV-1 origin replication. Transfer of the MAbs into mammalian cells was carried out by electroporation (6). The transfection conditions were optimized to a level which allowed the uptake of both DNA and protein into CHO4.15 cells, which constitutively express viral E1 and E2 proteins (31) (see Materials and Methods). The presence of the relatively high concentrations of antibodies in the transfection mixture had no effect on CHO4.15 cell growth. We estimated that 1 to 2% of the input antibody was taken up by the cells under these conditions, as determined by Western blot analysis. Analysis of the cells immediately and 24 and 48 h after transfection showed structurally intact immunoglobulin heavy chains within the cells, indicating that no active degradation of the transfected antibodies took place in the cells (data not shown).

View larger version (16K): [in this window] [in a new window]   FIG. 4.   Effect of E2-specific MAbs on papillomavirus replication. (A) CHO4.15 cells constitutively expressing BPV-1 E1 and E2 proteins were electroporated with 100 ng of reporter plasmid pUCAlu and various concentrations of MAbs. Cells were harvested 72 h after electroporation. Episomal DNA was digested with DpnI and linearizing enzyme HindIII and analyzed by Southern blotting. The replication signals of three independent experiments were quantified with a PhosphorImager, and signals from cells transfected with the origin-containing plasmid only were used as a control to normalize the results. Symbols: , MAb 3F12; , MAb 1E4; , MAb 1H10; , MAb 5H4; , nonspecific anti--galactosidase (-gal) MAb. (B) Southern blot analysis of transient replication of the BPV-1 origin-containing plasmid pUCAlu in the CHO4.15 cell line in the presence of MAbs at a concentration of 80 µg/ml. Episomal DNA was extracted from cells 72 h after transfection. Filters were probed with radiolabelled plasmid pUCAlu. (C) Western blot analysis of E2 protein levels in transfected CHO4.15 cells with rabbit anti-E2 polyclonal antibody.

Effect of Fab' fragments on DNA replication. Our results showed that MAbs 1H10, 1E4, 3F12, and 5H4 suppressed BPV-1 origin replication in a dose-dependent fashion (Fig. 4A). At the same time, MAbs 1H10, 3F12, and 1E4 did not influence the formation of the E1-E2-origin complex and supershifted this complex efficiently (Fig. 3, lanes 3 to 5). These data suggest that MAb 3F12 (epitope at residues 199 to 206) and MAbs 1H10 and 1E4, directed against the hinge region of the E2 protein, would not interfere directly with E1 and E2 interactions with DNA; however, these antibodies can sterically interfere with the inter- or intramolecular interactions required for the replication activity of the E2 protein. In order to study the possibility that antibodies would have an effect on replication due to steric hindrance of the formation of the replication initiation complexes, the Fab' fragments of MAbs 3F12, 1H10, and 5H4 were prepared by a modified procedure (see Materials and Methods). An ELISA with E2-coated microtiter plates showed that all of the Fab' fragments were active in binding to the E2 protein. The affinities of the Fab' fragments of 1H10 and 5H4 were similar, while the Fab' fragment of 3F12 had a lower affinity, as determined by titration on the ELISA plates (data not shown).

View larger version (19K): [in this window] [in a new window]   FIG. 5.   Effect of E2-specific Fab' fragments on DNA replication. (A) Ability of MAb 5H4 and its Fab' fragment to inhibit the formation of the E2-DNA complex at various antibody concentrations. The mobility shift assay was carried out with 2 ng of bacterially expressed and purified E2 protein and 0.2 ng of radiolabelled E2 binding site for 15 min at room temperature. MAb 5H4 or its Fab' fragment was added after E2 was mixed with its DNA target, and incubation was carried out for an additional 20 min. (B) Inhibition of DNA replication by E2-specific Fab' fragments at various concentrations. Reporter plasmid pUCAlu (100 ng) was cotransfected together with the Fab' fragment of 3F12 (), the Fab' fragment of 1H10 (), or the Fab' fragment of 5H4 () into cell line CHO4.15. At 72 h after electroporation, cells were harvested, and episomal DNA was digested with DpnI and linearizing enzyme HindIII and analyzed by Southern blotting. The replication signals of three independent experiments were quantified with a PhosphorImager, and signals from cells transfected with the origin-containing plasmid only were used as a control to normalize the results. (C) Southern blot analysis of transient replication of the BPV-1 origin-containing plasmid pUCAlu in the CHO4.15 cell line in the presence of Fab' fragments at a concentration of 0.3 mg/ml. Episomal DNA was extracted from cells either 48 or 72 h () after transfection. Filters were probed with radiolabelled plasmid pUCAlu.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

The crystal structure of the DNA-binding domain of the E2 protein with and without DNA has been solved (15, 16). Until the crystal structure of the full-length E2 protein is determined, we will have to rely on other methods to examine the structural organization of the whole protein and the molecular interactions that must occur to accomplish the replication and/or transcription activity of the protein. Even if the crystal structure were known, information about possible interactions should be gathered by other methods. In this study, we have produced and characterized a panel of MAbs as probes and tools for studying the structure and function of the BPV-1 E2 protein.

A total of 22 MAbs that were reactive to the E2 protein in an enzyme immunoassay were isolated. Seventeen of these MAbs were directed against linear epitopes that were mapped within the region between amino acids 180 and 309 of E2. In fact, the last part of the amino-terminal transactivation domain and the first 10 amino acids of the hinge region, residues 180 to 218, appear to constitute a highly immunogenic "hot spot," since epitopes for 12 of these 17 MAbs were found to be localized within this region. The reason for the highly immunogenic properties of the region between residues 180 and 218 is unknown. Epitopes for 5 of the 22 MAbs were mapped within the C-terminal DNA-binding and dimerization domain. Interestingly, all of these antibodies recognized the composite epitopes and did not react with the denatured E2 protein. None of the epitopes for the MAbs tested were mapped to the first 180 residues of the E2 protein.

When only a purified transactivation domain, residues 1 to 218, of E2 was used for immunization, four MAbs against the region between amino acids 1 and 180 of E2 were obtained; however, none of them was able to recognize the E2-DNA complex in a mobility shift assay (21a). In contrast, Hibma and coworkers (17) raised antibodies against the N-terminal part of the HPV-16 E2 protein, indicating that the HPV-16 and BPV-1 E2 proteins are considerably different in terms of structure and epitope presentation. The most antigenic regions are usually the less ordered regions of the protein without packed internal side chains. From this point of view, the differential antigenicity may be a reflection of the differences in the structures of the HPV-16 and BPV-1 E2 proteins. Gauthier and coworkers (10) probed the structure of HPV-16 E2 with polyclonal antibodies raised against synthetic peptides that cover the whole region of the HPV-16 E2 protein. They found that antipeptide antibodies against the hinge region but not against the transactivation domain or the DNA-binding and dimerization domain were able to recognize the native form of the HPV-16 E2 protein.

In our study, MAb 1E2 (epitope within residues 184 to 190) was able to recognize neither E2-DNA nor E1-E2-origin complexes in a mobility shift assay. Curiously, deletion of the first alpha helix from the BPV-1 E2 protein revealed the epitope for MAb 1E2, and the protein in the protein-DNA complex was recognized by the antibody. Thus, the epitope for this MAb is probably buried within the compact structure of the N-terminal domain and is not accessible unless the structure of the molecule is distorted in some fashion. These data suggest that the transactivation domain of the E2 protein, unlike many other transactivation domains, has remarkable structural integrity. As shown by X-ray analysis, the C-terminal DNA-binding and dimerization domain has a compact structure (15). Deletion of the last 13 C-terminal residues of E2 resulted in an inactive protein unable to bind DNA and support replication (21a). So, our data confirm that in a native context, both the transactivation domain and the DNA-binding and dimerization domain of BPV-1 E2 have a complex and relatively rigid structure, while the central, hinge region is highly mobile and flexible.

MAb 5H4 and its Fab' fragment efficiently inhibited the formation of both E2-DNA and E1-E2-origin complexes. They not only competed with DNA for binding but also were able to dissociate the preformed E2-DNA complex. In a transient replication assay, MAb 5H4 and its Fab' fragment efficiently suppressed BPV-1 DNA replication. This assay is another way to demonstrate that the BPV-1 E2 protein interaction with the specific recognition sequence within an origin of replication is essential for the initiation of viral DNA replication. In addition, the results indicate that it is possible to target the E2 protein interaction with DNA for therapeutic purposes by using this specific MAb or Fab' fragment to block the replication of papillomaviruses.

In our study, MAb 3F12 (epitope at residues 199 to 206), directed against the last 10 amino acids of the transactivation domain, and MAbs that bind the hinge region, 1H10 (epitope at residues 208 to 218) and 1E4 (epitope at residues 250 to 280), efficiently suppressed BPV-1 DNA replication. However, the Fab' fragments of 1H10 and 3F12 had no inhibitory effect on and even activated replication. None of these antibodies interfered with the formation of the E1-E2-origin complex. These data suggest that antibodies 3F12 and 1H10 sterically hindered the inter- or intramolecular interactions required for the replication activity of the E2 protein. On the other hand, our results demonstrate the importance of the hinge region for the replication activity of the E2 protein. This hypothesis is based on results from several laboratories. E2 proteins containing large internal in-frame deletions of the hinge region (from amino acids 195 to 309, 213 to 309, and 220 to 309) were not able to support DNA replication (or had a decreased efficiency) but could efficiently enhance the binding of E1 to the replication origin (46, 47). A fusion protein which contained the transactivation domain together with the hinge region of BPV-1 E2 linked to the GCN4 DNA-binding domain supported replication much more efficiently than a fusion protein in which only the transactivation domain of E2 was linked to the GCN4 DNA-binding domain (3). These data suggest that the conformational freedom of the E2 protein is important for its role in replication and that the inhibitory effect of the antibodies against the epitopes in the hinge region as well as in the very last part of the transactivation domain may be explained by interference with conformational freedom, which would not allow E2 to assume the proper conformation required for its replication activity. However, another possibility is that this region is important for interactions with replication factors, so that antibodies that bind to the first part of the hinge region can but Fab' fragments cannot prevent the binding of replication factors to the same region of the E2 protein.