INTRODUCTION

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Virus persistence requires that infection occur with little or no cytolysis (1). Such asymptomatic infections may be a stable feature of the virus-host interaction (e.g., arenavirus infections [7]), or they may alternatively arise during a cytopathic infection by changes that reduce cytolytic potential (e.g., reovirus infections [18]). In vitro and in vivo infections by murine hepatitis virus (MHV) can readily shift from acutely cytolytic to persistent asymptomatic phases (6, 37, 49, 54). Thus, genetic and biochemical determinants of cytolytic potential can be revealed through studies of MHV infections.

Determinants of lytic potential can be categorized into virus-encoded and cell-encoded groups. With respect to the viral determinants, numerous correlations between changes in MHV genome sequence and MHV-induced cytopathology have been made (22, 32, 33, 56). These studies were tenable for two reasons; first, the 32-kb RNA genome of MHV is subject to spontaneous mutation (41); second, such mutants can be isolated, sequenced, and assessed for pathogenic potential. Most mutations correlating with changes in virus-induced pathology localize to the 4.1-kb open reading frame encoding the major surface (S) glycoprotein of the virus.

Current understanding of the biological functions of the S protein supports the contention that changes in this viral protein might affect the pathology of infection. The S protein forms the most prominent projections of the coronavirus particle (16). These projections are essential for delivery of the MHV genome into cells. They bind to cellular receptors (12, 27), and they additionally carry out the virion-cell membrane fusion event that occurs subsequent to receptor binding (55). Finally, there is definitive evidence from expression of mutated cDNAs that changes in S structure will alter its receptor binding and membrane fusion properties (28, 29), and these changes are indeed correlated with profound changes in virus-induced cytopathology (14, 22).

Cellular determinants of lytic potential appear somewhat varied and are best understood in the context of S-protein function. For example, the lipid composition of cellular membranes impacts MHV-induced cytopathology (17, 47). This is not surprising in light of our understanding that S proteins become cytopathic as they accumulate on the surface of infected cells and begin to mediate intercellular fusion (12). However, this fusion (and hence cytopathic effect) is generally inhibited by increases in the unsaturated fatty acid content of membranes (9, 47). The protease content of host cells also impacts MHV-induced cytopathology (23). Mechanistic explanations of this finding appeal to S-protein structure and function; S proteins undergo a proteolytic cleavage event during exocytosis through the Golgi apparatus, and oligomers comprised of the cleaved S products (S1 and S2) induce cytopathic membrane fusion more effectively than their uncleaved precursors (53).

Cellular receptors for MHV play an obvious role in virus-induced cytopathology. The primary receptor (termed MHVR [21]) molecules are required to initiate infection as they are specifically recognized by the S proteins protruding from the virion membrane (19, 20). MHVR also promotes expansion of infection between cells, as S proteins on infected cells bind to MHVR on neighboring cells, thereby promoting syncytia. Recent studies of tissue culture cells persistently infected with MHV reveal relatively few virus-induced syncytia and, at the same time, relatively low levels of MHVR (11, 48). Thus, it is conceivable that reduced levels of MHVR on cells might be responsible for reduced virus-induced cytopathology, but such a hypothetical relationship is far from clear as no defined culture has been developed in which MHVR levels could be adjusted.

Thus, we developed a set of HeLa cell-MHVR transfectants that vary only in their levels of intracellular and surface MHVR. Using these cell lines, we found that the MHVR densities required for infection by virions were in fact lower than those that promote development of syncytia. With these cell lines, we made the additional observation that virus-induced cytopathology increases with increasing receptor levels. We found that the S protein, when produced in the absence of the other coronavirus proteins, will rapidly kill only those HeLa cells that produce abundant MHVR. Furthermore, we found that this cell death does not require the formation of syncytia; the death in fact occurs in well-separated cells and thus may arise by formation of S-MHVR complexes within intracellular organelles.

	MATERIALS AND METHODS

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Cells and viruses. HeLa-tTA (tTA denotes tetracycline-controlled transactivator) (35), HeLa-MHVR (29), and rabbit kidney clone 13 (RK13) cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco-BRL Corp.). EBNA-sMHVR-Ig (28) cells were grown in DMEM-10% FBS containing the antibiotics G418 (100 µg/ml) and hygromycin B (200 µg/ml). Murine 17 cl 1 cells (51) were grown in DMEM containing 5% tryptose phosphate broth (Difco Laboratories) and 5% FBS. MHV strain A59 (MHV-A59) was grown in 17 cl 1 culture, and virions were purified as previously described (29). Infectivities of MHV-A59 were determined by plaque assay, using HeLa-MHVR (line 5) as indicator cells. Recombinant vaccinia viruses were grown and titered in RK13 cell cultures.

Quantitation of MHVR levels following doxycycline exposure. To alter MHVR levels, doxycycline (Sigma Co.) was added to the growth medium above HeLa-MHVR (line 5) cells at concentrations from 10³ to 10⁰ µg/ml for various time periods. Cell surface MHVR levels were then measured by fluorescence-activated cell sorting (FACS). To this end, approximately 10⁶ cells were incubated with 5 µl of monoclonal antibody (MAb) CC1 (58) in a volume of 250 µl of phosphate-buffered saline (PBS)-2% bovine serum albumin (BSA) for 30 min on ice. Cells were then pelleted, and unbound CC1 was removed by three cycles of resuspension and repelleting in ice-cold PBS. Rinsed cell pellets were resuspended in 100 µl of PBS-2% BSA containing 1 µl of fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse immunoglobulin G (IgG) antibody (Cappell, Inc.). After 30 min on ice, unbound antibody was removed and the final pellets were suspended in 1 ml of PBS. Fluorescence profiles were obtained using a FACStar Plus (Becton Dickinson Co.), and the calculated mean fluorescence intensities (MFI) were taken as indicators of the cell surface MHVR levels.

Quantitation of MHVR levels by immunoblot analysis. HeLa-tTA or HeLa-MHVR cells (10⁸) were dissolved in 1 ml of radioimmunoprecipitation assay (RIPA) buffer (10 mM Tris-HCl [pH 7.2], 150 mM NaCl, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate [SDS], 1% Triton X-100). Serial dilutions were prepared with RIPA buffer as diluent, and each sample (10 µl) was mixed with 20 ng of sMHVR-Ig (28), which served as an internal quantitation standard. These samples were then electrophoresed on SDS-polyacrylamide gels (40) and transferred to nitrocellulose filters. Authentic MHVR (110 kDa) and sMHVR-Ig (48 kDa) were identified on the filters by primary incubation with MAb CC1 (0.1%) in binding buffer (PBS-3% BSA-0.1% Tween 20) for 2 h at 22°C. Filters were then incubated with 0.1% goat anti-mouse Ig-alkaline phosphatase conjugate (Cappell) in binding buffer for 2 h at 22°C. Immobilized alkaline phosphatase was enzymatically detected with 5-bromo-4-chloro-3-indolylphosphate-toluidinium nitroblue tetrazolium reagent (Pierce Co).

Indirect immunofluorescence. Adherent HeLa-MHVR cells were grown on glass coverslips and then infected with MHV-A59 at various multiplicities. At 8 or 12 h postinfection, the cells were washed with PBS and then fixed alternatively in acetone or in PBS-3.7% formaldehyde. Formaldehyde-fixed cells were permeabilized for 10 min in PBS-0.1% Triton X-100. After rinsing with PBS, cells were incubated for 1 h at 22°C in PBS-2% BSA containing a 1:250 dilution of antimatrix MAb 5A5.2 (12). The bound MAb was detected with FITC-conjugated goat anti-mouse IgG antibody (1:1,000 in PBS-2% BSA), and cells were photographed with a Leitz fluorescence microscope.

Quantitative intercellular fusion assay. The cell fusion-dependent reporter gene (-galactosidase) activation assay of Nussbaum et al. (45) was performed as described earlier (46), with minor modifications. Briefly, HeLa-MHVR^lo cells were coinfected with MHV-A59 and with vaccinia virus strain WR, each at a multiplicity of infection (MOI) of 10 PFU/cell. After 1 h, these cells were further transfected by lipofection with the reporter gene construct pGINT7-gal (kindly provided by Richard A. Morgan, National Center for Human Genome Research, Bethesda, Md.). Five hours later, the cells were overlaid onto confluent 5-cm² monolayers of HeLa (or HeLa-MHVR) cells that had been inoculated 12 h earlier with recombinant vaccinia virus vP11 (2). At hourly intervals thereafter, the mixed monolayers were lysed by addition of 0.5% Nonidet P-40 (NP-40) in PBS and the quantity of -galactosidase was calculated as described earlier (46).

Single-cell lysis. Trypan blue exclusion was used as an indicator of cell viabilities. The measurements were made on subconfluent HeLa-MHVR cell monolayers (10⁴ cells/cm²) that were infected as described in the text. In all experiments, rabbit anti-A59 serum MK15 (kindly provided by Susan Baker, Loyola University Medical Center) was added at 3 h postinfection, to a final concentration of 1%. This addition prevented the syncytium formation that might otherwise occur to a limited extent by ~8 h postinfection. Quantitations were performed at 14 h postinfection by trypsinizing adherent cells and suspending them along with the nonadherent populations into DMEM-10% FBS. Cells were pelleted, resuspended in PBS-0.2% trypan blue (Gibco BRL), and counted. Each experimental condition was assayed in triplicate, and a minimum of 150 total cells were counted in each condition.

Electron microscopy. Transmission electron microscopic studies were done on HeLa-MHVR cell monolayers (10⁴ cells/cm²) that were infected with MHV-A59 (10 PFU/cell). At 10 h postinfection, monolayers were fixed in PBS containing 4% glutaraldehyde for 2 h at 4°C, then scraped from the dishes with a rubber policeman, pelleted by centrifugation, and washed with PBS by resuspension and repelleting. The final cell pellets were postfixed in PBS containing 1% osmium tetroxide for 1 h at 22°C and then treated with 0.1 M sodium cacodylate (pH 7.4) containing 1% tannic acid for 0.5 h at 22°C. Cells were repelleted, dehydrated in acetone, and embedded in Embed 812 (EM Sciences, Inc.). Sections of 70 nm were prepared on copper grids and stained for 10 min at 22°C with 5% uranyl acetate and then with Reynold's lead citrate for 5 min at 22°C. Sections were examined in a Hitachi H-600 electron microscope (70 kV).

Coimmunoprecipitation of S protein-MHVR protein complexes. To allow for the synthesis of the S protein within cells producing MHVR, HeLa-MHVR monolayers (10⁶ cells/10 cm²) were coinfected with vTF7.3 (26) and vTM1-S_JHM (32) for 1 h at 2 PFU/cell. Where indicated, infected cells were radiolabeled with [³⁵S]methionine (Tran³⁵S-label; ICN Pharmaceuticals, Inc.) from 3 to 7 h postinfection. Cell lysis was then achieved by using PBS-0.5% NP-40 at 22°C. Nuclei were removed by centrifugation, and cytoplasmic extracts (0.5 ml/10⁶ cells) were mixed with 5 µl of rabbit antipeptide serum 874 (kindly provided by Michael J. Buchmeier, Scripps Research Institute) and with 10 µl of 50% (vol/vol) Sepharose-protein G beads (Pharmacia Biotech). After a 2-h incubation at 4°C, beads were washed by sequential resuspension and repelleting, using first ice-cold buffer A (0.01 M Tris-HCl [pH 7.2], 1 M NaCl, 0.1% NP-40), then buffer B (0.01 M Tris-HCl [pH 7.2], 0.1 M NaCl, 0.3% SDS, 0.1% NP-40, 0.001 M EDTA), and then buffer C (0.01 M Tris-HCl [pH 7.2], 0.1% NP-40). Where indicated, the final bead pellets were resuspended in endoglycosidase H buffer (0.05 M sodium phosphate [pH 6.5], 0.1% SDS, 1 mM phenylmethylsulfonyl fluoride). Samples in the endoglycosidase H buffer were incubated for 4 h at 37°C in the presence of 0.5 mU of endoglycosidase H (Boehringer Mannheim). Radiolabeled proteins were removed from the beads by addition of sample solubilizer (40) and a 5-min incubation at 100°C. Proteins were separated on SDS-polyacrylamide gels, impregnated with Entensify reagent (DuPont Corp.), and visualized by fluorography.

	RESULTS

Top Abstract Introduction Materials & Methods Results Discussion References

Regulated production of MHVR. Preliminary studies of a series of HeLa-MHVR transfectants that we developed in our laboratory (29) indicated that they varied substantially in MHVR production levels. Each cell line also varied in its capacity to serve as an indicator of MHV-A59 infectivity. In some transfectant lines, MHV-A59 produced large clear plaques; in others, only small pinprick plaques were observed. These basic observations suggested that MHVR levels influence MHV-A59 infection and syncytium formation.

View larger version (7K): [in this window] [in a new window]   FIG. 1.   Schematic depiction of the tetracycline-controlled expression system. tTA is a constitutively synthesized recombinant protein containing separate domains for transcriptional activation (A) and for DNA binding (R). Addition of tetracycline (T) to culture medium releases the tTA from operator DNA (TetO7), thereby preventing transcription of MHVR cDNA from the minimal cytomegalovirus promoter (P).

View larger version (14K): [in this window] [in a new window]   FIG. 2.   Time required for doxycycline to change surface MHVR levels. Stably transfected HeLa-MHVR cells (line 5) were incubated in DMEM-10% FBS, either with or without doxycycline, and the cell surface MHVR levels were measured by flow cytometric analysis. The mean of fluorescence intensity for each sample was calculated and plotted after subtraction of background (HeLa-tTA cell) fluorescence. (A) Addition of 1 µg of doxycycline per ml at time zero; (B) removal of doxycycline at time zero from cultures exposed for the prior 2 weeks in 1 µg of doxycycline per ml.

3

View larger version (26K): [in this window] [in a new window]   FIG. 3.   FACS profiles of HeLa-MHVR cells treated with graded doses of doxycycline. Cells were incubated in DMEM-10% FBS containing 0 (A), 0.001 (B), 0.01 (C), 0.1 (D), and 1 (E) µg of doxycycline per ml for 2 weeks at 37°C. Cell surface MHVR levels were measured as described in Materials and Methods. (F) FACS profiles of untransfected HeLa-tTA cells.

View larger version (26K): [in this window] [in a new window]   FIG. 4.   Quantitation of MHVR content of Rhi and Rlo cells by Western immunoblotting. The HeLa-MHVR cells were solubilized with detergent-containing RIPA buffer, and the indicated cell equivalents were mixed with 20 ng of sMHVR-Ig (28) prior to electrophoresis and transfer to nitrocellulose. Immobilized MHVR was identified using antireceptor MAb CC1 (58) and alkaline phosphatase-conjugated second antibody as described in Materials and Methods.

Comparisons of the R^lo and R^hi cells following murine coronavirus infection. (i) Both R^lo and R^hi cells can be infected. The cultures, which varied only in the intracellular and cell surface density of MHVR, provided us with the opportunity to directly assess the role of receptor abundance in virion infection and syncytium formation. To assess this infection susceptibility, we challenged the cultures with MHV-A59, and at 8 h postinfection we identified cells producing the viral matrix protein by indirect immunofluorescence. We found that all the MHVR cultures, from R^hi to R^lo, contained matrix-positive (MHV-A59-infected) cells (Fig. 5). As expected, the proportion of infected cells increased with increasing MHVR levels, from 4% for R^lo to 62% for R^hi, at an MOI of 1 PFU/cell. These variations in infection susceptibility could not be attributed to the doxycycline used to modulate receptor levels because the same doxycycline administrations to murine 17 cl 1 cultures did not alter either the number of A59-infected cells or the progeny virus yields (data not shown).

View larger version (22K): [in this window] [in a new window]   FIG. 5.   Quantitation of infected HeLa-MHVR cells following challenge with MHV-A59. HeLa-MHVR cells were infected with MHV-A59 at the indicated multiplicities and 8 h later fixed in acetone and stained for the presence of the viral matrix protein by the immunofluorescence procedure described in Materials and Methods. The percentages of matrix-positive cells were calculated by counting at least 250 total cells for each experimental condition. Error bars represent standard deviations from the mean of three independent determinations.

(ii) The R^lo cells are resistant to virus-induced syncytium formation. Microscopic examination of immunostained R^lo monolayers at 14 h after A59 infection (100 PFU/cell) revealed the complete absence of virus-induced syncytia. R^hi cells infected in parallel were fused into a single unbroken syncytium (Fig. 6A and B). Similar observations were made in cultures inoculated at the lower input multiplicities of 1 and 10 PFU/cell (data not shown). That R^lo cells were sensitive to virion infection but resistant to syncytium formation suggested that virion entry required less cell surface MHVR than that required to promote intercellular fusion and thus warranted a closer examination. Therefore we quantitated the effect of MHVR levels on the promotion of virus-induced intercellular fusion by using a previously developed assay of cytoplasmic mixing (45, 46). The assay involved the introduction of the transcriptionally silent T7-gal reporter plasmid into a population of A59-infected R^lo cells. After the display of the S protein on the plasma membrane, these cells were overlaid onto monolayers of R^lo or R^hi cells previously infected with vP11 (2), which encodes T7 RNA polymerase. Intercellular fusion resulting from the S-MHVR interaction would then cause cytoplasmic mixing and subsequent transcription of the -galactosidase gene by the T7 polymerase.

View larger version (61K): [in this window] [in a new window]   FIG. 6.   Measurement of syncytia in MHV-A59-infected Rhi and Rlo cells. (A and B) Cells were processed as described in the legend to Fig. 5 at 8 h after MHV-A59 infection (MOI = 100 PFU/cell) and photographed with a Leitz fluorescence microscope. (C) The indicated cell monolayers were infected with vaccinia virus recombinant vP11, which causes T7 RNA polymerase to accumulate within the cells; 12 h later, these monolayers were overlaid with cells from a separate Rlo culture that was infected/transfected 5 h earlier with MHV-A59, vaccinia virus strain WR, and the -galactosidase-encoding plasmid pT7--gal (45). These cells contain S protein on plasma membranes and transcriptionally silent -galactosidase genes in cytosol. At hourly intervals following the overlay, cultures were lysed in PBS-0.5% NP-40 (5 × 105 cells/ml/well), and the quantities of -galactosidase were determined by an enzymatic assay.

(iii) The R^lo cells secrete high yields of progeny virus. We and others have found that abundant MHVR synthesis decreases the yields of infectious MHV-A59 particles (10, 30), but a complete mechanistic understanding of this inhibition is not yet available. To further investigate the role of MHVR levels during virus production, we monitored the time course of progeny virus development (Fig. 7). Interestingly, our findings indicated that the yields from both R^lo and R^hi cultures were similar up to 9 h postinfection, but after this time, yields from R^lo cells continued to increase while those from R^hi cells declined (Fig. 7A). We initially suspected the reduced virus production in R^hi cultures might be due to the cytopathology brought on by cell-cell fusion; by 8 h postinfection, R^hi cultures existed as a continuous macroscopic syncytium beginning to detach from its plastic substrate (Fig. 6A). However, this syncytium formation could not account for the low virus yields at the later time periods. Even in cultures seeded at densities low enough to prevent intercellular contact (10⁴ cells per cm²), similar time courses were observed. R^hi cells supported virus secretion to 9 h postinfection and then progressively lost this ability. R^lo cells continued to secrete increasing amounts of progeny to 15 h postinfection (Fig. 7B). These findings led us to speculate that virus infection of the R^hi cells, but not the R^lo cells, resulted in an acute single-cell death that was not dependent on the pathologic effects of syncytium formation.

View larger version (24K): [in this window] [in a new window]   FIG. 7.   Yields of MHV-A59 secreted from infected Rhi and Rlo cells. Following inoculation with MHV-A59 (MOI = 10 PFU/cell), cultures were incubated in DMEM-10% FBS (1 ml/106 cells). At 3 h intervals, the DMEM-10% FBS was removed and replaced with fresh medium. Viral infectivities in the spent media were determined by plaque assays using HeLa-MHVRhi cells as indicators.

(iv) The R^hi cells undergo an acute single-cell death following MHV-A59 infection. To investigate whether receptor levels affect the viabilities of infected cells, we inoculated subconfluent monolayers of R^hi and R^lo cells with MHV-A59 and then made two straightforward measurements. Viable cells were measured by trypan blue exclusion, and infected cells were measured by in situ immunostaining of the viral matrix protein. To ensure that any loss of cell viability was not due to syncytium formation, we added a fusion-blocking antiviral antiserum (MK15 rabbit antiserum) at 3 h postinfection. At the 1% concentration used, this antiserum blocked all fusion and additionally prevented superinfection by progeny virions (data not shown).

View larger version (20K): [in this window] [in a new window]   FIG. 8.   Rapid virus-induced cell death in Rhi (but not Rlo) cells. Infection of subconfluent cells with MHV A59 (MOI = 100 PFU/cell) was followed 3 h later by addition of hyperimmune anti-A59 rabbit serum MK15 to 1% (final concentration). At 14 h postinfection, cells were suspended by using trypsin, and trypan blue was used to monitor cell lysis (white bars). Infected cells (black bars) were scored by indirect immunofluorescence as described in Materials and Methods. Error bars represent standard deviations from the average of four independent experiments.

(v) Ultrastructural analyses reveal a loss of organelle integrity in the infected R^hi cells. The infection process was rapidly cytotoxic only in the R^hi cells, and this toxicity was evident even in the absence of cell-to-cell contact. These findings prompted us to hypothesize that intracellular perturbations (i.e., formation of MHVR-S complexes within organelles) correlate with the rapid cell death. Thus, we set out to determine whether the presence of high MHVR levels during virus infection grossly affects intracellular architecture. We first used electron microscopic methods to visualize organelle integrity in the infected cells. Using this technique, we visualized vesicles loaded with virus particles (thin arrows) in both the R^lo and R^hi cells at 10 h postinfection (Fig. 9). However, only the R^hi cells showed obvious signs of necrosis. Cytoplasmic vacuoles and swelled, crista-damaged mitochondria were readily evident in the R^hi cells (thick arrows). There was no evidence of nuclear blebbing and chromatin condensation by this method (compare uninfected and infected sections), which suggested that the cell death we observed was not due to apoptosis (8, 39, 43).

View larger version (151K): [in this window] [in a new window]   FIG. 9.   Transmission electron micrographs of MHV-A59-infected HeLa-MHVR cells. Cells were processed for microscopy at 10 h postinfection (MOI = 10 PFU/cell). Thin arrows point to virus-containing vesicles; thick arrows point to vacuoles. N, nucleus. (A) Mock-infected Rhi; (B) A59-infected Rhi; (C) A59-infected Rlo. Bars represent 0.5 µm.

View larger version (42K): [in this window] [in a new window]   FIG. 10.   Distribution of the viral matrix protein in MHV A59-infected HeLa-MHVR cells. Subconfluent monolayers were processed for indirect immunofluorescence at 12 h postinfection (MOI = 100 PFU/cell) and photographed with a Leitz fluorescence microscope. Exposure times were identical. (A) Rhi; (B) Rlo.

The viral spike protein will induce single-cell death in R^hi cells. Given that numerous findings implicate the MHV S protein in virus-induced cytopathology (14, 22) and given that the S protein is the only virus-encoded protein known to bind to MHVR (27), it was reasonable to suspect that acute cytolysis of R^hi cells would occur if S proteins were synthesized even in the absence of other coronavirus proteins. This possibility was addressed by constructing vaccinia virus vectors capable of producing the S proteins of various MHV strains and infecting R^lo or R^hi cells with these vectors.

View larger version (18K): [in this window] [in a new window]   FIG. 11.   Measurement of single-cell death induced by production of the MHV S protein. Subconfluent densities of HeLa-MHVR cells were coinfected with vTF7.3 along with the indicated vTM1-based recombinants encoding various forms of the MHV spike (SJHM, Secto, S1, SOBL, and SA59). At 3 h postinfection, the fusion inhibiting antibody MK15 was added. Cells were trypsinized at 12 h postinfection, and the number of viable cells was determined by trypan blue exclusion. Error bars represent standard deviations from the mean obtained from six independent experiments.

Spike and receptor proteins form intracellular complexes. Our findings on the effect of the S protein on R^lo and R^hi cell viability suggested to us that cytolysis results from the accumulation of S-MHVR complexes within intracellular compartments. Thus, we set out to identify such complexes by examining whether antireceptor antibodies might coimmunoprecipitate both the MHVR and S proteins. This approach was tenable because we had access to a monospecific antipeptide antibody (R874) directed against the carboxy-terminal 16 amino acids of the MHVR that we had previously determined to be effective and specific in its ability to immunoprecipitate the receptor (46). Because this antibody recognized an epitope that was distant from the portion of the MHVR that binds to S (20), we reasoned that it would be exposed on both free MHVR and MHVR that was associated with the S protein.

View larger version (26K): [in this window] [in a new window]   FIG. 12.   Coimmunoprecipitation of MHVR and S from cells producing both proteins. (A) Rhi or Rlo cells were coinfected with vaccinia virus recombinants vTF7.3 and vTM1-SJHM and then radiolabeled with Tran[35S]-label from 3 to 7 h postinfection. Immediately after labeling, cytoplasmic extracts were prepared by the NP-40 lysis procedure, and 35S-labeled proteins were immunoprecipitated with antireceptor antiserum and Sepharose-protein G. Proteins bound to the Sepharose-protein G were treated with endoglycosidase H (+) or left untreated () and then prepared for SDS-polyacrylamide gel electrophoresis. The separated proteins were visualized by fluorography. Lane 1, Rhi cells (vTF7.3 plus VVWR) (control; no S produced); lanes 2 and 3, Rhi cells (vTF7.3 plus vTM1-SJHM); lane 4, Rlo cells (vTF7.3 plus vTM1-SJHM); lanes 5 and 6, Rhi cells (vTF7.3 plus vTM1-Secto. (B) Rhi cells were coinfected with vTF7.3 and vTM1-SJHM. At 12 h postinfection, cells were lysed along with exogenously added 35S-labeled A59 virions (12 × 104 cpm) and then immunoprecipitated with antireceptor antibody as described above. Precipitations were performed in the absence or presence of exogenously added sMHVR, which specifically absorbs S proteins to Sepharose-protein G (28). Immunoprecipitates were subjected to SDS-polyacrylamide gel electrophoresis and fluorography. Lane 7, vTF7.3 plus VVWR; lanes 8 and 9, vTF7.3 plus vTM1-SJHM.

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

MHV infections exhibit remarkable variability in cytopathology, ranging from acutely cytolytic to essentially asymptomatic levels in cultured cells (13, 42). This range of MHV-induced cytopathology has generally been considered to be dependent on the variable activity of the S protein. S proteins can induce a cytotoxic intercellular membrane fusion, the potency of which depends on numerous factors including the capacity of newly synthesized S proteins to fold, assemble, and transport to the cell surface, the susceptibility of S to proteolytic cleavage by host cell proteases (23), the lipid composition of the host cell (9, 47), and the pH of the extracellular environment (48). This study has identified the receptor to which S proteins bind (the MHVR) as yet another determinant of MHV-induced cytopathology. In fact, in our system involving HeLa-MHVR transfectant cell lines as hosts for MHV infection, the level of receptor production was the primary determinant of virus-induced cytopathology. In our system, the synthesis and processing of the S proteinindeed, the entire productive infection processwas not in and of itself powerfully cytotoxic. Rather, it was the concomitant synthesis of two proteins, S and MHVR, that accounted for the virus-induced death of the HeLa-MHVR cells.

Regulated production of the MHVR in HeLa-MHVR transfectants allowed us to determine that the amount of cell surface MHVR required for infection by incoming virions was lower than that required to promote membrane fusion between infected cells. Thus, R^lo cells could be infected and could support development of progeny virions for prolonged periods without suffering from the toxic effects accompanying syncytium formation. This finding that virions, but not infected cells, could successfully fuse with the R^lo cells suggested that the two membrane fusion events have unique requirements. Distinct requirements were actually considered likely, as previous studies of retro- and paramyxoviruses have shown that the virion-cell fusion process can readily occur under conditions in which the cognate cell-cell fusion is limited. Virion-cell fusion can occur at low temperature (3), in the presence of syncytium-blocking peptides (38), and at relatively low receptor levels (4, 50). Hints that the virion-cell fusion during coronavirus infection might similarly occur more readily than the corresponding cell-cell fusion is suggested by its exclusive occurrence even when viral S proteins remain largely as relatively inactive, uncleaved S precursors (33). To explain our observation of successful virion-cell fusion at low MHVR levels, we suggest that S proteins must be closely spaced for fusion to occur and that such close spacing is found on the virion surface but not the infected cell surface. We further hypothesize that MHVR levels must be relatively high to congregate the S proteins found on the infected cell surface into the close proximities necessary to promote fusion. Such a hypothesis would be consistent with previous findings indicating that virus-induced membrane fusion requires the formation of arrays of nearby viral glycoproteins (15, 24, 57).

We initially thought that the cytotoxicity arising from abundant MHVR levels was due solely to its role in promoting the intercellular fusion events that ultimately produce syncytia (i.e., Fig. 6). Thus, we were surprised to find that our R^hi cells died within ~14 h following infection even when separated from each other (Fig. 8). We obtained similar results when we infected the HeLa-MHVR cells with vaccinia virus-S recombinants (Fig. 11). This led us to conclude that the abundance of MHVR strongly influenced the cytotoxicity of endogenously synthesized S proteins by mechanism(s) that were independent of syncytium formation.

Numerous findings lead us to suggest that the coexpression of S and MHVR in cells could generate cytotoxic, intracytoplasmic membrane fusion events. First, only complete, membrane-anchored forms of S were able to cause the death of individual, well-separated R^hi cells (Fig. 11). Soluble S ectodomain fragments that cannot induce membrane fusion (S1 or the complete S1/S2 ectodomain) were far less cytotoxic, even though they bound avidly to the MHVR within the cell (Fig. 12). Second, complete S proteins that were identical to those produced by the weakly fusogenic MHV strains A59 and OBL (31, 32) were less cytotoxic than the corresponding highly fusogenic JHM S proteins (Fig. 11), indicating that toxicity and fusion potentials are correlated. Third, the intracellular architecture in cells producing both MHVR and S in abundance was clearly distinct from those cells in which lower amounts of MHVR were produced (Fig. 9 and 10); this raises the possibility of fusion between or within intracellular vesicles. Finally, we were able to specifically immunoprecipitate complexes of S and MHVR from infected R^hi lysates, and we could document their predominance in premedial Golgi locations (Fig. 12). Thus, we hypothesize that the accumulation of these complexes impairs intracellular integrity of organelles and ultimately causes necrosis and cell death.

In contrast to the R^hi cells, our R^lo cells survive MHV infection without ever undergoing any acute cytolytic phase, and they continuously secrete high titers of progeny (Fig. 7 and data not shown). Notably, we found that our infected R^hi cells would similarly develop into persistently infected cultures, but only after an acute phase of infection that destroyed all but the few cells (<1%) producing low levels of MHVR; i.e., cells repopulating the cultures were R^lo (data not shown). Thus, the maintenance of persistent infection requires establishment of cultures in which MHVR levels are low. This important finding has been previously documented in studies of persistently infected murine cell cultures (11, 48).

Is it possible that the relative levels of MHVR in vivo might help to explain the nature of MHV-induced cytopathologies? One might speculate that such is the case given what is currently known about in vivo MHVR levels and their relationship to sites of persistent virus infection. MHVR levels in the murine central nervous system are substantially lower than those found in peripheral organs such as the liver and intestine (58, 59), and persistent virus infection is generally restricted to the central nervous system. These findings justify consideration of MHVR levels as one of many factors determining the pathology of in vivo infection. Since the tetracycline-controlled system used in our studies is adaptable to the in vivo environment (5, 34), this interesting question may soon be addressed through experimentation.