INTRODUCTION

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Many cytolytic animal viruses, including rotavirus (13, 20), are capable of establishing persistent infections of cultured cells. In most cases, the mechanisms by which these persistent infections are maintained involve selection of mutant cells that do not efficiently support viral replication, selection of mutant viruses that are less cytopathic, or coevolution of mutant cells and viruses such that cellular resistance to viral replication is balanced by an enhanced capacity of the virus to infect the resistant cells (reviewed in reference 1). Viral entry has been shown to be a critical point of virus-cell interaction for the selection of mutations during persistent infections caused by several viruses, including coronavirus (11, 24), poliovirus (7, 31), and reovirus (17, 49). It is not known whether mutant viruses and cells are also selected during persistent rotavirus infections or whether such mutations affect rotavirus entry.

Rotaviruses are nonenveloped, icosahedral viruses that contain a genome consisting of 11 segments of double-stranded RNA (reviewed in reference 19). Rotavirus replication is initiated by attachment of the virus to cell surface receptors, which have not been identified with certainty. The attachment step is mediated by outer capsid protein VP4 (5, 16, 29, 32, 35, 38, 43), a spike protein that extends approximately 120 Å from the virion surface (41, 45, 52). The mechanism by which rotavirus enters cells is not precisely understood; however, current data suggest that rotavirus entry occurs by direct penetration of the virus into the cytosol. Rotavirus virions increase membrane permeability as measured by the release of radioactive chromium from cells (28) and the release of fluorescent dyes from liposomes (39) and isolated membrane vesicles (44). In addition, rotavirus virions induce fusion from without in cultured cells (22). Treatment of rotavirus with the intestinal protease trypsin significantly enhances viral infectivity (2, 3, 14, 18, 21), most likely by increasing the efficiency of membrane penetration (22, 28, 39, 44). Trypsin cleaves VP4 into two fragments, VP5* and VP8* (15, 18, 21, 34). VP5* is postulated to contain a fusion domain that facilitates rotavirus penetration into cells (36); VP8* contains sequences that mediate hemagglutination (23, 38) and likely participate in viral attachment to nucleated cells.

To better understand mechanisms of persistent rotavirus infections of cultured cells and to determine whether virus-cell coevolution involving viral entry occurs during persistent rotavirus infection, we characterized viruses and cells from MA104 cultures persistently infected with simian rotavirus strain SA11. These cultures produced high titers of infectious virus for prolonged periods and were cured by passage in medium containing anti-rotavirus antibodies. Viruses isolated from the persistently infected cultures and cells cured of persistent infection were tested to determine whether mutations in viruses and cells were selected during propagation of these persistent infections. Our findings suggest that persistent rotavirus infections are carrier cultures maintained by horizontal transmission of virus between cells and that the requirement for VP4 cleavage is a target for selection of mutant viruses and cells during maintenance of persistent rotavirus infection.

	MATERIALS AND METHODS

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Cells and viruses. MA104 cells were obtained from Biowhittaker (Walkersville, Md.) and grown in a 5% CO₂ atmosphere at 37°C in Eagle medium (Sigma Chemical Co., St. Louis, Mo.) that was supplemented to contain 10% fetal bovine serum (FBS) (Intergen, Purchase, N.Y.), 2 mM L-glutamine, 500 U of penicillin per ml, and 500 µg of streptomycin per ml (Irvine Scientific, Santa Ana, Calif.). Cells were passaged when confluent by using 1% trypsin with 5 mM EDTA (Irvine Scientific). Rotavirus strain SA11 was obtained from the American Type Culture Collection. Viruses isolated from persistently infected cultures of MA104 cells were designated PI viruses. Both wild-type (wt) and PI viruses were plaque purified twice in MA104 cells prior to preparation of lysate stocks (42). Virus stocks were grown in MA104 cells, which were maintained in Eagle medium without FBS and supplemented to contain 2 µg of porcine trypsin (Intergen) per ml. Purified virion preparations were made by infecting MA104 cells with trypsin-treated (20 µg of trypsin per ml, incubated at 37°C for 30 min) third-passage lysate stocks as previously described (10), with the exception that freon-extracted virus from the cell pellet and virus from the supernatant were collected by centrifugation through a 40% sucrose solution. To obtain purified rotavirus virions containing ³⁵S-labeled proteins, MA104 cells were maintained in complete Eagle medium without FBS for 2 h after adsorption with trypsin-treated virus. The medium was removed, and methionine-free Eagle medium (Sigma) without FBS was added. Following incubation for 1 h, Easytag [³⁵S]methionine (DuPont NEN, Boston, Mass.) (0.1 mCi per ml) was added to the medium and cells were incubated for an additional 1 h. After that time, the medium was removed and cells were maintained in complete Eagle medium without FBS and without trypsin for 20 h. The concentration of viral particles in preparations of purified virions was determined by using the conversion 1 optical density unit at A₂₆₀ equals 2.1 × 10¹² viral particles (46).

Establishment of persistently infected MA104 cell cultures. Persistent rotavirus infections were established by infecting confluent MA104 cell monolayers (2 × 10⁶ cells), at the 60th passage level, with independent third-passage lysate stocks of SA11 at a multiplicity of infection (MOI) of 0.1 PFU per cell (Table 1). The viral inocula were treated with 20 µg of porcine trypsin per ml at 37°C for 30 min prior to adsorption. The cultures were maintained in complete Eagle medium without FBS for the first 2 to 3 days, after which time the medium was supplemented to contain 10% FBS. Cells were either passaged when confluent or supplemented with fresh medium every seventh day if the cell density was not sufficient to permit passage. Trypsin was used to passage the cultures, but additional trypsin was not added to the medium. Cell culture lysates (5 ml per flask) were collected at each passage by two cycles of freezing and thawing (70°C and 37°C).

Determination of viral titer in persistently infected cell culture lysates. Viral titer in cell culture lysates was determined by plaque assay as previously described (42). Samples were treated with 10 µg of trypsin per ml, diluted serially 10-fold, and adsorbed to MA104-cell monolayers in duplicate in 6-well plates (Costar, Cambridge, Mass.). The MA104 cells were then overlaid with Eagle medium (Sigma) supplemented to contain 2 mM L-glutamine, 500 U of penicillin per ml, 500 µg of streptomycin per ml, 2 µg of trypsin per ml, and 1% agar (Difco, Detroit, Mich.) and incubated at 37°C. Plaques were counted on day 4 after staining with 1% neutral red (Fisher Scientific, Pittsburgh, Pa.).

Preparation of polyclonal antirotavirus antiserum. Polyclonal rabbit antirotavirus antiserum was obtained by inoculating a New Zealand White rabbit with 100 µg of purified SA11 virions in complete Freund's adjuvant, followed by booster doses of 50 µg of purified virions in incomplete Freund's adjuvant at 2, 3, and 7 weeks after the initial inoculation (Cocalico, Reamstown, Pa.). Antiserum was heat-inactivated by incubation at 56°C for 30 min prior to use. A 1:5,120 dilution of antiserum was sufficient to achieve a 90% reduction in infectivity of SA11 as determined by plaque-reduction neutralization assay (51).

Infectious center assay. Cells were washed three times in phosphate-buffered saline and enumerated. Cells were diluted serially 10-fold and deposited onto MA104-cell monolayers in duplicate in 6-well plates. After adsorption at room temperature for 2 h, cells were overlaid with complete Eagle medium containing 1% agar and processed according to the plaque assay technique (42).

Immunocytochemical staining with antirotavirus antibody. Cells were grown in chambered glass slides (Lab Tek, Nunc, Naperville, Ill.) and fixed with 1:1 (vol/vol) acetone-methanol at 4°C for 5 min. Viral antigen was detected by using the VectaStain Elite ABC kit (Vector Laboratories, Burlingame, Calif.) according to the manufacturer's instructions. The primary antibody was a 1:100 dilution of a rotavirus group A-specific monoclonal antibody (Serotec, Ebetsu, Japan) directed against VP6 (48). The chromogen was 3,3'-diaminobenzidine tetrahydrochloride (0.04%) in 0.05 M Tris-HCl (pH 7.4) and 0.025% H₂O₂.

Isolation of cells cured of persistent rotavirus infection. MA104 cells were cured of persistent rotavirus infection by passage for 35 days in medium supplemented to contain 1% rabbit antirotavirus antiserum. Antibody-treated cells (designated MX) were tested for viral infection by plaque assay of cell culture lysates (42), infectious center assay, immunocytochemistry, reverse transcription-PCR amplification (RT-PCR) of rotavirus RNA, and electron microscopy.

Detection of rotavirus RNA by RT-PCR. Parental MA104 cells, MA104 cells persistently infected with rotavirus, or MX cells cured of persistent infection (5 × 10⁶ cells) were centrifuged to form a pellet, resuspended in 3.0 ml of lysis buffer (1% sodium dodecyl sulfate [SDS], 1 mM EDTA, and 50 mM Tris [pH 8.0]), and incubated at 37°C for 15 min. Lysates were extracted three times with 1:1 (vol/vol) phenol-chloroform after the addition of 60 µl 5 M NaCl. Nucleic acid was precipitated in 3 volumes of cold 100% ethanol after the addition of 1/10th volume of 3 M ammonium acetate (pH 5.2). Total cellular nucleic acid (2 µg) was used as template for RT-PCR (35 cycles of cDNA amplification). Nucleic acid was incubated in 10 µl 40% dimethyl sulfoxide at 95°C for 2 min and then added to ice-cold oligonucleotide primers and incubated on ice for 5 min prior to RT-PCR. Cycling parameters were set such that melting of cDNA template strands occurred at 95°C for 1 min, annealing of primers and cDNA template occurred at 45°C for 2 min, and synthesis of cDNA occurred at 72°C for 3 min. Oligodeoxynucleotide primers 5'-TAACTATTGTGCTCATAGAG and 5'-ATGTTCAAGATGGAGTCTAC corresponding to nucleotide sequences of rotavirus SA11 gene segment 7 (8) were used in these reactions. This primer pair is expected to amplify a 999-base-pair cDNA. Rotavirus genomic double-stranded RNA purified from SA11 virions and cellular nucleic acid purified from parental MA104 cells served as controls. RT-PCR products were resolved in a 1.0% agarose-Tris-borate-EDTA gel and visualized by ethidium bromide staining.

Electron microscopy. Cells were centrifuged to form a pellet (1,000 × g for 3 min) and suspended in phosphate-buffered 2% glutaraldehyde. After primary fixation, cells were again centrifuged (1,000 × g for 3 min), resuspended in 1% osmium tetroxide, dehydrated in propylene oxide and increasing percentages of ethanol from 50% to 100%, and then embedded in an epoxy resin. Ultrathin sections were prepared with an LKB Ultratome III ultramicrotome and stained with lead citrate and uranyl acetate. Sections were examined in a Philips 300 electron microscope.

Growth of wt and PI rotaviruses in parental MA104 cells and cured MX cells. Monolayers of parental MA104 cells or cured MX cells (10⁵ cells) were infected with rotavirus strains at an MOI of 2 PFU per cell in 24-well plates (Costar). Prior to adsorption, the viral inoculum was either untreated or treated with 2.5 to 20 µg of trypsin per ml at 37°C for 30 min. Virus was adsorbed to cells at 4°C for 1 h, the inoculum was removed by washing twice with phosphate-buffered saline, and 1.0 ml of trypsin-free, fresh medium was added. After incubation at 37°C for 24 h, cells were frozen and thawed twice and virus in cell lysates was titrated on MA104 cell monolayers by plaque assay (42).

SDS-PAGE of rotavirus structural proteins. Rotavirus virions containing [³⁵S]-labeled proteins were purified from infected MA104 cells (10), either untreated or treated with 1.25 to 10 µg of trypsin per ml at 37°C for 30 min, and subjected to SDS-polyacrylamide gel electrophoresis (PAGE) (33). In preparation for electrophoresis, virion samples were mixed 1:1 with 2× sample buffer (250 mM Tris [pH 6.8], 4% 2-mercaptoethanol, 2% SDS, 20% sucrose, and 0.02% bromophenol blue) and incubated at 65°C for 5 min. After incubation, samples were loaded into wells of an 8% polyacrylamide gel and electrophoresed at 200 V constant voltage for 45 min. Gels were fixed, dried onto filter paper under vacuum, and exposed to Kodak BiomaxMR film (Eastman Kodak Co., Rochester, N.Y.).

	RESULTS

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Establishment of persistently infected MA104 cell cultures using rotavirus strain SA11. To determine whether rotavirus can establish persistent infections of MA104 cells, four independent cultures of these cells were infected with trypsin-treated inocula of independent, plaque-purified, third-passage lysate stocks of rotavirus strain SA11 (Table 1). The cultures were either passaged when cells reached confluence or supplemented with fresh medium every 7 days. Trypsin was used to passage the cultures, but additional trypsin was not added to the medium. Establishment of the persistently infected cultures was associated with intense cell crises in which only a few colonies of cells in the cultures survived. Recovery until first passage was prolonged, varying from 20 to 48 days, after which time no significant crises were observed, including times when the cultures were treated with trypsin to facilitate passage. Viral titers in lysates collected during passage of the four independent, persistently infected cultures varied from 10⁴ to 10⁷ PFU per ml for 350 days (data not shown). To assess cell viability, an aliquot of each culture was stained with trypan blue at several passages (Table 2 and data not shown). After the initial period of crisis, 72 to 100% of cells were viable as assessed by trypan blue exclusion. Therefore, MA104 cell cultures persistently infected with rotavirus can be established, and these cultures produce infectious virus for at least 350 days of culture maintenance.

Cure of persistent rotavirus infection of MA104 cells. To assess whether persistent rotavirus infection of MA104 cells can be cured by passage in medium containing antirotavirus antibodies, the persistently infected MA104/SA11-A culture (subsequent to the 11th passage) was maintained in medium containing antirotavirus antiserum. Antibody treatment was continued for 35 days (4 passages), after which time the resulting culture, termed MX-S, was passaged in medium without antiserum. During the period of antibody treatment, viral titer decreased to less than 10 PFU per ml of culture lysate (the lower limit of detection) and remained undetectable for greater than 30 days after completion of antibody treatment (Fig. 1 and data not shown). We also tested whether the antibody-treated cells could give rise to infectious centers. In these experiments, no infectious centers were produced by the MX-S culture per 5 × 10⁵ cells 24 days after completion of antibody treatment. In addition, antibody-treated cells did not contain immunocytochemically detectable rotavirus antigen 30 days after completion of antibody treatment (data not shown). Thus, antirotavirus antibody treatment of a persistently infected MA104 cell culture eradicates infectious virus and viral antigen from the culture.

View larger version (23K): [in this window] [in a new window]   FIG. 1.   Viral titer in cell culture lysates obtained from persistently infected MA104/SA11-A and cured MX-S cells. On the indicated day of culture maintenance, the titer of infectious virus in cell culture lysates was determined by plaque assay using MA104 cells. The period of antibody treatment (day 210 to day 245) is indicated. (Inset) RT-PCR to detect rotavirus-specific RNA in persistently infected and cured MA104 cell cultures. Total cellular nucleic acid was purified from each culture, and approximately 2 µg was used as template for RT-PCR (35 cycles of cDNA amplification). Oligodeoxynucleotide primers corresponding to the SA11 gene segment 7 sequence (8) were used in these reactions; genomic double-stranded RNA purified from SA11 virions and cellular nucleic acid purified from parental MA104 cells served as controls. RT-PCR products were resolved in a 1.0% agarose-Tris-borate-EDTA gel and visualized by ethidium bromide staining. Positions of DNA size markers are shown on the left in kilobase pairs. The primers used in these reactions are expected to amplify a 999-base-pair cDNA.

Ultrastructure of MA104 cells persistently infected with rotavirus and cured of persistent infection. To define the location of virus in the persistently infected cells and to ascertain whether changes in cellular ultrastructure are associated with persistent rotavirus infection, we used electron microscopy to examine the morphology of uninfected MA104 cells (Fig. 2A), persistently infected MA104/SA11-A cells (Fig. 2B), and cured MX-S cells (Fig. 2C). In comparison to uninfected cells, both persistently infected and cured cells exhibited a polarized appearance: villouslike projections were observed on one surface, while the other surface was smooth. In addition, both types of cells contained increased numbers of mitochondria and vacuoles. These morphological features were observed in all thin sections examined. These features were not observed in uninfected MA104 cells passaged in medium containing antirotavirus antibodies for a 35-day period as a control (data not shown). Particles consistent with rotavirus virions were visualized in <5% of cells from the persistently infected cultures and were contained in membrane-bound structures (Fig. 2D). Rotavirus virions were not observed in cured MX-S cells.

View larger version (120K): [in this window] [in a new window]   View larger version (135K): [in this window] [in a new window]   FIG. 2.   Morphology of uninfected, persistently infected, and cured MA104 cells. (A) Uninfected MA104 cells. (B) Persistently infected MA104/SA11-A cells. (C) Cured MX-S cells. (D) Particles consistent with rotavirus virions enclosed in a membrane-bound structure in a persistently infected MA104/SA11-A cell. Bars, 1 µm.

Growth of wt and PI rotaviruses in parental MA104 cells and cured MX cells. To test whether mutant viruses and cells are selected during persistent rotavirus infections of MA104 cells, independent PI viruses were tested for growth in parental MA104 cells and cured MX-S cells. Monolayers of parental and cured cells at approximately the same passage number were infected with trypsin-treated inocula of wt SA11, PI-SA11-B/135, and PI-SA11-D/128 at an MOI of 2 PFU per cell (Fig. 3). After 24 h of viral growth in cured cells, the PI viruses produced 10- to 25-fold greater yields than that of wt SA11, which indicates that mutant viruses capable of enhanced growth were selected during propagation of the persistently infected cultures. In addition, wt SA11 produced 10-fold greater yields in parental cells than in cured cells, which demonstrates that mutant cells manifesting a block to rotavirus replication were also selected during these persistent infections. Thus, these results indicate that mutations arise in both viruses and cells during maintenance of MA104 cell cultures persistently infected with rotavirus.

View larger version (13K): [in this window] [in a new window]   FIG. 3.   Growth of wt and PI viruses in parental MA104 cells and cured MX cells. Monolayer cultures of MA104 cells and MX-S cells (5 × 105 cells) were infected with wt SA11, PI-SA11-B/135, and PI-SA11-D/128 at an MOI of 2 PFU per cell. Prior to adsorption, each viral inoculum was treated with 10 µg of trypsin per ml at 37°C for 30 min. After 1 h of adsorption, the inoculum was removed, 1.0 ml of fresh trypsinfree medium was added, and cells were incubated at 37°C for 24 h. Virus in cell lysates was titrated by plaque assay using MA104 cells. The results are presented as the mean viral yields (titer at 24 h divided by titer at 0 h) for four independent experiments. Error bars indicate standard deviations of the means.

Growth of PI rotaviruses with and without trypsin pretreatment. To determine whether mutations in viruses and cells selected during persistent rotavirus infections of MA104 cells affect viral entry, we tested the capacity of wt and PI SA11 viruses to infect MA104 cells in the absence of trypsin pretreatment. For these experiments, virus stocks were prepared without trypsin supplementation of the medium. Monolayers of parental MA104 cells were infected with wt SA11 and PI-SA11-B/135 at an MOI of 2 PFU per cell (Fig. 4). Prior to adsorption, the viral inoculum was either untreated or treated with trypsin at concentrations of from 5 to 20 µg per ml. In the absence of trypsin pretreatment, PI-SA11-B/135 produced approximately 50-fold greater yields than wt SA11 after 24 h of viral growth. As the concentration of trypsin used to treat the viral inoculum was increased, yields of wt SA11 approached those of PI-SA11-B/135 in the absence of trypsin pretreatment. However, increasing concentrations of trypsin did not increase yields of PI-SA11-B/135. Therefore, wt and PI rotaviruses differ substantially in the requirement for trypsin pretreatment to efficiently infect MA104 cells, suggesting that PI viruses have acquired mutations that alter steps in viral entry.

View larger version (16K): [in this window] [in a new window]   FIG. 4.   Growth of wt and PI viruses with and without trypsin pretreatment. Monolayer cultures of MA104 cells (105 cells) were infected with wt SA11 and PI-SA11-B/135 at an MOI of 2 PFU per cell. Prior to adsorption, each viral inoculum was treated with trypsin at the concentrations shown at 37°C for 30 min. After 1 h of adsorption, the inoculum was removed, 1.0 ml of fresh trypsinfree medium was added, and cells were incubated at 37°C for 24 h. Virus in cell lysates was titrated by plaque assay using MA104 cells. The results are presented as the mean viral yields (titer at 24 h divided by titer at 0 h) for four independent experiments. Error bars indicate standard deviations of the means.

Evolution of the capacity of PI rotaviruses to infect MA104 cells without trypsin pretreatment. To determine when in the course of persistent infection the capacity for trypsin-independent viral growth evolved, we measured viral yields after infections were initiated with untreated cell culture lysate stocks collected over time from the persistently infected MA104/SA11-B and MA104/SA11-D cultures (Fig. 5). The capacity of viruses in the persistently infected cultures to grow in MA104 cells without trypsin pretreatment was not a property of the virus stocks used to establish persistent infection but instead was gradually selected in these cultures by passage day 100. Thus, the property of trypsin-independent viral growth evolves during maintenance of persistent rotavirus infection of MA104 cells.

View larger version (22K): [in this window] [in a new window]   FIG. 5.   Effect of day of cell culture maintenance on the capacity of viruses from the persistently infected MA104 cell cultures to grow without trypsin pretreatment. Monolayers of MA104 cells (105 cells) were infected with cell culture lysates (0.2 ml of lysate stock) obtained from the persistently infected MA104/SA11-B and MA104/SA11-D cultures. Prior to adsorption, each viral inoculum was not treated with trypsin. After 1 h of adsorption, the inoculum was removed, fresh trypsinfree medium was added, and cells were incubated at 37°C for 24 h. Virus in cell lysates was titrated by plaque assay using MA104 cells. The results are presented as the mean viral yields (titer at 24 h divided by titer at 0 h) for two independent experiments.

SDS-PAGE analysis of structural proteins of wt and PI rotaviruses. Our finding that PI rotaviruses are capable of trypsin-independent growth prompted us to test whether these viruses contain cleaved VP4 proteins. Radiolabeled virions of wt SA11 and PI-SA11-B/135 were purified from lysate stocks prepared without trypsin and subjected to SDS-PAGE and autoradiography (Fig. 6). Prior to electrophoresis, virions were either untreated or treated with trypsin at concentrations of from 1.25 to 10 µg per ml. In the absence of trypsin treatment, bands corresponding to uncleaved VP4 were observed for both wt and PI viruses, indicating that wt and PI viruses prepared without trypsin contain intact VP4 proteins. Using these electrophoresis conditions, the VP4 protein of PI-SA11-B/135 migrated slightly slower than that of wt SA11. After trypsin treatment of purified virions of wt and PI viruses, bands of equivalent electrophoretic mobility corresponding to trypsin-cleavage fragment VP5* were observed. In these experiments, there was no discernible difference between wt and PI viruses in the relative decrease in VP4 band intensity with increasing concentrations of trypsin. Thus, PI virus VP4 proteins are uncleaved in stocks prepared without trypsin but remain susceptible to trypsin-mediated proteolysis.

View larger version (61K): [in this window] [in a new window]   FIG. 6.   SDS-PAGE of structural proteins of wt and PI viruses. Purified [35S]methionine-labeled virions of wt SA11 and PI-SA11-B/135 at a concentration of 2 × 1011 particles per ml were treated with trypsin at the concentrations shown (in micrograms per milliliter) at 37°C for 30 min. Equal volumes of samples (2 × 109 particles) were loaded into wells of an 8% SDS-polyacrylamide gel. After electrophoresis, gels were prepared for fluorography and exposed to film. Viral proteins are labeled on the left, and positions of molecular size markers are shown on the right (in kilodaltons).

Effect of trypsin pretreatment on growth of PI rotaviruses in cured MX cells. Since PI viruses produced equivalent yields in parental MA104 cells with and without trypsin treatment of the viral inoculum, we tested the effect of trypsin pretreatment on growth of PI viruses in cured MX cells. Monolayers of MX-S cells were infected with PI-SA11-B/135 and PI-SA11-D/128 at an MOI of 2 PFU per cell with and without trypsin pretreatment (Fig. 7). After 24 h of viral growth, trypsin-treated PI viruses produced approximately 40-fold greater yields than untreated viruses. Therefore, PI viruses require trypsin pretreatment to efficiently infect cured MX cells but not parental MA104 cells.

View larger version (11K): [in this window] [in a new window]   FIG. 7.   Effect of trypsin pretreatment on growth of PI viruses in cured MX cells. Monolayer cultures of MX-S cells (105 cells) were infected with PI-SA11-B/135 and PI-SA11-D/128 at an MOI of 2 PFU per cell. Prior to adsorption, each viral inoculum was either untreated or treated with 10 µg of trypsin per ml at 37°C for 30 min. After 1 h of adsorption, the inoculum was removed, 1.0 ml of fresh trypsinfree medium was added, and cells were incubated at 37°C for 24 h. Virus in cell lysates was titrated by plaque assay using MA104 cells. The results are presented as the mean viral yields (titer at 24 h divided by titer at 0 h) for four independent experiments. Error bars indicate standard deviations of the means.

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

MA104 cells can be persistently infected with rotavirus. In this report, we show that MA104 cell cultures infected with rotavirus strain SA11 can support persistent infection for prolonged periods. After an initial period of crisis in which only a few cells survived, the cultures stabilized and produced substantial titers of infectious virus for 350 days of continuous passage. Treatment of persistent rotavirus infection of MA104 cells with antirotavirus antibodies resulted in cure of viral infection, which suggests that horizontal transmission of virus between cells is required for propagation of persistent rotavirus infection. During maintenance of the persistently infected cultures, mutant cells were selected that do not support efficient growth of wt virus and mutant viruses were selected that can infect the resistant cells. Furthermore, mutant viruses selected during these persistent infections do not require trypsin pretreatment to productively infect parental MA104 cells but do so to infect MA104 cells cured of persistent infection. These observations suggest that mutations in viruses and cells affecting a trypsin-sensitive step in rotavirus entry coevolve during maintenance of persistent infection of MA104 cells.

Persistent rotavirus infections of MA104 cells are carrier cultures in which viruses and cells coevolve. The finding that MA104 cell cultures persistently infected with rotavirus can be cured by treatment with neutralizing antirotavirus antibodies suggests that these cultures are carrier cultures maintained by horizontal transmission of virus between cells (17, 37). Neutralizing antibodies are believed to block early steps in viral replication, such as attachment, penetration, or disassembly (reviewed in reference 50). Rotavirus infectivity is neutralized by antibodies directed against outer capsid proteins VP4 and VP7 (6, 25, 26, 30, 47), and monoclonal antibodies directed against VP4 block viral attachment (43) and penetration (28). These steps would be required to maintain horizontal transmission of virus in a persistently infected culture. Therefore, the capacity of neutralizing antibodies to eradicate persistent infection suggests that lateral viral spread is required to maintain viral infection in these cultures.

Mutations in PI viruses confer trypsin-independent growth. Since trypsin-mediated proteolysis of VP4 is required for efficient entry of rotavirus into cells (22, 28, 39, 44), we reasoned that viruses having altered requirements for trypsin pretreatment might be selected during maintenance of the cultures. We found that PI viruses produce substantially greater yields than wt virus in parental MA104 cells in the absence of trypsin pretreatment. The trypsin-independent phenotype was selected in both persistently infected cultures tested and required approximately 100 days of culture maintenance to become fully manifest. These findings suggest that a protease requirement for efficient viral growth is a point of balance for viral growth and cellular survival during maintenance of persistent infection.