INTRODUCTION

Top Abstract Introduction Materials & Methods Results Discussion References

A nef gene is found in all subgroups of primate lentiviruses (49, 53). nef is considered a nonessential auxiliary gene because it can be deleted without dramatically affecting the ability of virus to replicate in cell culture (31, 56, 57). Evidence for the importance of nef for the efficiency of viral replication in the intact organism and for the maintenance of high virus loads has been derived both from studies with simian immunodeficiency virus (SIV) in monkeys and from studies with human immunodeficiency virus type 1 (HIV-1) in humans. Monkeys infected with a derivative of a pathogenic molecular clone of SIV from which nef gene sequences were specifically removed maintain low or undetectable virus loads and usually show no signs of disease progression (31). Similarly, one human in central Massachusetts (32) and several in Australia (16) are infected with nef-deleted forms of HIV-1, and they too are long-term nonprogressors who maintain low viral loads. In Australia, a single blood donor clearly transmitted nef-deleted HIV-1 to several recipients via blood donations, and this virus clearly behaved with a markedly attenuated phenotype.

Information is beginning to emerge which suggests that nef may have evolved a number of different, independent functional activities to enhance the replication and survival of virus. These include downregulation of the CD4 receptor from the surface of the cell (1, 21, 43, 50), downregulation of major histocompatibility complex class I molecules (52) which may protect infected cells from killing by cytotoxic T lymphocytes (15), infectivity enhancement (2, 38), and lymphocyte activation (3, 6, 17, 18) or inhibition of lymphocyte activation (23, 26, 39). Each of these functional activities clearly involves interactions with the host cell. Finding the cellular partners that couple with nef to achieve these functional activities is important for defining the biochemical activities and eventually delineating their relative importance. Cellular proteins that have been found to couple with nef include src family kinase (5, 14, 34, 46), a serine/threonine kinase (24, 37, 51), protein kinase C (PKC) theta (55), -cop (7), a thioesterase (36), and CD4 (45).

In this report, we describe the specific interaction of the zeta chain of the T-cell receptor (TCR) with nef of SIVmac, SIVsm, and HIV-2. Specific binding to TCR was not observed with five different nef alleles of HIV-1. An active tyrosine kinase was found to coprecipitate with the nef-TCR complex, suggesting that the interaction might influence T-cell signaling.

	MATERIALS AND METHODS

Top Abstract Introduction Materials & Methods Results Discussion References

Cell lines and plasmids. The Jurkat human T-cell line and the J.CAM1.6 cell line were obtained from the American Type Culture Collection (Rockville, Md.) and grown in RPMI 1640 medium which contained 25 mM HEPES, 10% fetal calf serum (Gibco/BRL, Grand Island, N.Y.), penicillin-streptomycin (50 IU and 50 µg/ml, respectively), and 2 mM L-glutamine (Gibco/BRL). Cos 18 cells were kindly provided by A. Weiss (Howard Hughes Medical Institute, University of California, San Francisco, Calif.) and maintained in Dulbecco's modified Eagle's medium supplemented with 5% fetal calf serum, 0.4 mg of G418 per ml, penicillin-streptomycin (50 IU and 50 µg/ml, respectively), and 2 mM L-glutamine (Gibco/BRL). The 221 cell line was grown in RPMI 1640 medium supplemented with 5% fetal calf serum, 10% interleukin-2 (IL-2), penicillin-streptomycin (50 IU and 50 µg/ml, respectively), and 2 mM L-glutamine (Gibco/BRL).

Yeast two-hybrid screen. Yeast two-hybrid screening was performed according to the protocol suggested by the MATCHMAKER TWO-HYBRID SYSTEM 2 (Clontech, Palo Alto, Calif.). nef hybrid expression plasmid pBD-239nef2 was constructed by fusing a truncated SIVmac239 nef gene encoding amino acids (aa) 1 to 15 and aa 98 to 263 (nef2; see Fig. 1) to the GAL4-DNA binding domain in the pAS2-1 vector. nef2 was used for the yeast two-hybrid screen in order to minimize high, nonspecific backgrounds seen with the full-length nef and because nef sequences missing aa 16 to 97 are still capable of associating with a serine/threonine kinase (data not shown) (51). A cDNA library from phytohemagglutinin (PHA)-activated human lymphocytes fused to the GAL4-DNA activation domain was purchased from Clontech. The yeast strain Saccharomyces cerevisiae Y190 (leu his trp auxotroph) harboring the two reporter genes HIS3 and lacZ was sequentially transformed with the nef hybrid expression plasmid and the PHA-activated human lymphocyte cDNA library by using the lithium acetate transformation method (Clontech). Double transformants were plated onto synthetic medium agar plates lacking leucine, tryptophan, and histidine in the presence of 3-amino-1,2,4-triazole (LYH+3AT). After 10 days of incubation at 30°C, His⁺ colonies were rescued and patched onto LYH+3AT plates. -Galactosidase activities in these His⁺ colonies were tested by replica plating on nylon filters which were dipped into liquid nitrogen, soaked in 5-bromo-4-chloro-3-indolyl--D-galactopyranoside (X-Gal) buffer, and incubated at room temperature for 3 to 5 h. Colonies of the LacZ⁺ clones were restreaked onto LYH+3AT plates to isolate single colony and were retested for -galactosidase activity. Confirmed positive clones (His⁺ LacZ⁺) were grown in leucine-minus synthetic medium in the presence of 10 µg of cycloheximide per ml for 5 days at 30°C to counterselect pBD-239nef2. Plasmids from the segregants (Leu⁺ Trp Cyh^r) containing only the pAD-cDNA were isolated, transformed into Escherichia coli, and sequenced.

Yeast mating assay. To verify specific interaction of nef and the protein expressed by the cDNA clones, S. cerevisiae Y190 containing the pAD-cDNA was mated with another strain, Y187, previously transformed with the pAS2-1 fused to the wild type or the truncated SIV nef genes in complete YPD medium for 18 h at 30°C. Diploid yeast cells were plated onto LYH+3AT plates and incubated for 5 days at 30°C. His and LacZ phenotypes were scored as described above.

Construction of Nef expression plasmids. The plasmid p239SpE3' containing the 3' half of the SIVmac239 open proviral genome (42) was used as the template for the PCR amplification of the truncated nef fragments. nef1, nef2, and nef3 fragments were amplified by PCR by using the 5' primers AH1 (5'-GGAAGATCTGGGACAGTATATGAATACTCCATG-3'), AH2 (5'-GGAAGATCTGGGGGTATCAGTGAGGCCAAAA-3'), and AH3 (5'-GGAAGATCTGTACAGTGCAAGAAGACATAGA-3') and the 3' primer 3' NefPsIAU1 (5'-ACG CTGCAGTTATATATAGCGATAGGTGTCGCGAGTTTCCTTCTTGTCAGC- 3'), respectively, which introduced the unique BglII and PstI restriction sites (underlined) and a sequence encoding an AU1 epitope tag (in boldface). nef4, nef5, and nef6 fragments were amplified by using the 5' primer 5'NefEcoRI (5'-GCGGAATTCATGGGTGGAGCTATTTCCATG-3') and the 3' primers AH4 (5'-ACGCTGCAGTTATATATAGCGATAGGTGTCGCTTCCAAACTCTTCT GGGTA-3'), AH5 (5'-ACGCTGCAGTTATATATAGCGATAGGTGTCTAGCCAGCCAAATGTCTTTGG-3'), and AH6 (5'-ACGCTGCAGTTATATATAGCGATAGGTGTCGTAACTCATTGTTCTTAGGGG-3'), respectively, which introduced the unique EcoRI and PstI restriction sites (underlined) and an AU1 epitope (in boldface). nef2-4 and nef3-4 were constructed by PCR amplification of p239SPE3' with the 5' primers AH2 and AH3, respectively, with the 3' primer AH4. The nef1, nef2, nef3, nef2-4, and nef3-4 fragments digested with BglII and PstI and the nef4, nef1, and nef6 fragments digested with EcoRI and PstI were cloned into pFJ-239nef (18) previously digested with the corresponding enzymes to create pFJnef1, pFJnef2, pFJnef3, pFJnef4, pFJnef5, pFJnef6, pFJnef2-4, and pFJnef3-4.

Expression and purification of recombinant glutathione S-transferase (GST)-fusion protein. Ten milliliters of overnight cultures of E. coli transformed with pGEX-4T or recombinant plasmids were diluted 1:20 with fresh medium and grown for 2 h at 37°C before inducing with 0.5 mM isopropyl--D-thiogalactopyranoside (IPTG). After a further 6 h of incubation, the cells were pelleted and resuspended in 10 ml of bacteria lysis buffer containing 1% Triton X-100, 0.1% N-lauryl sarconsinate, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 0.1% aprotinin, and 1 µg of leupeptin per ml in phosphate-buffered saline (PBS). Cells were lysed by sonication followed by centrifugation at 10,000 × g for 5 min at 4°C. The cell pellets were sonicated again and centrifuged at 10,000 × g for 15 min at 4°C. The supernatant was collected and incubated with 500 µl of the preswollen glutathione-Sepharose beads (Pharmacia) for 2 h at 4°C. The beads were washed three times with ice-cold PBS and stored at 4°C.

In vitro binding assay. A total of 10⁷ cells were lysed in 1 ml of cell lysis buffer (0.5% Nonidet P-40, 50 mM HEPES [pH 7.5], and 150 mM NaCl) containing 2 mM NaVO₃, 10 mM NaF, 1 mM PMSF, 1 µg of leupeptin per ml, and 1% aprotinin (Sigma Chemical, St. Louis, Mo.). The cell lysates were centrifuged at 13,000 × g for 30 min at 4°C. The supernatant was mixed with 30 µl of the glutathione-Sepharose beads (beads) and 20 µl of the immobilized GST (GST beads; 5 mg/ml) and incubated for 30 min at 4°C. Precleared cell extracts were incubated with approximately 30 µg of the soluble GST and 10 µl of the immobilized GSTnef fusion proteins or GST beads (all loaded beads contained approximately 2 mg of protein per ml) for 3 h at 4°C. The coprecipitated proteins were washed three times with ice-cold lysis buffer, boiled in Laemmli sample treatment buffer (33) for 5 min, separated by sodium dodecyl sulfate (SDS)-10% polyacrylamide gel electrophoresis (PAGE) and electroblotted onto the Immobilon membrane (Millipore, Bedford, Mass.). Immunodetection was performed with a 1:3,000 dilution of anti-TCR mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, Calif.) and developed by the enhanced chemiluminescence (ECL) system with procedures suggested by the manufacturer (Amersham, Chicago, Ill.).

Transfection, immunoprecipitation, and immunoblotting. Cos 18 cells were transfected with 5 µg of the nef expression plasmids by the standard DEAE-dextran method (47). Cells were harvested 48 h after transfection and lysed with 1 ml of the cell lysis buffer containing a cocktail of protease inhibitors as described above. The cell lysates were cleared by centrifugation at 13,000 × g for 30 min at 4°C and divided into aliquots of 50 and 950 µl, respectively. The aliquot containing 50 µl of the cell lysate was used for the analysis of nef protein expression, while the aliquot containing 950 µl of the cell lysate was used for the immunoprecipitation. Immunoprecipitation was performed by adding 1 µl of anti-AU1 mouse monoclonal antibody (BABCO Biotech, Berkeley, Calif.) and 30 µl of protein A-agarose (Santa Cruz Biotechnology) to the cell lysates. The suspension mixtures were rocked at 4°C for 3 h, and the immune complexes were washed three times with the cell lysis buffer and once with 50 mM Tris-HCl (pH 7.4). Immunoprecipitated proteins were separated by SDS-10% PAGE, transferred onto the Immobilon membrane (Millipore), and reacted with the anti-TCR mouse monoclonal antibody (diluted 1:3,000; Santa Cruz Biotechnology), and detected by the ECL system (Amersham).

	RESULTS

Top Abstract Introduction Materials & Methods Results Discussion References

SIVmac239 nef binds to a TCR sequence in yeast cells. We used a deleted form of SIVmac239 nef protein (aa 1 to 15 and 98 to 263; nef2 in Fig. 1) fused to the DNA binding domain of the GAL4 transcription factor as bait in a yeast two-hybrid screen. The expression plasmid for this fusion construct was called pBD-239 nef2. A cDNA library prepared from PHA-activated human lymphocytes was fused to the transcription activation domain of the GAL4 transcription factor (pAD-cDNA) and introduced into S. cerevisiae Y190 previously transformed with pBD-239nef2. When the protein encoded by pBD-239nef2 interacts with that encoded by pAD-cDNA, it will reconstitute a functional GAL4 transcription factor and activate transcription of two reporter genes, lacZ and HIS3, respectively, which are present in the S. cerevisiae Y190. Transformants harboring the positive interacting clones are expected to grow in the absence of histidine and to produce -galactosidase.

View larger version (14K): [in this window] [in a new window]   FIG. 1.   Schematic representation of deletion mutations in nef of SIVmac239. Sequence motifs are described by Shugars et al. (53) and Samuel et al. (49). Myr, myristoylation site; SH2, putative consensus SH2 binding sequence; p34cdc, consensus cyclin-dependent kinase substrate sequence; D-E, acidic stretch; PXXP, putative SH3 binding motif; Y-P, tyrosine kinase recognition sequence; PKC1 and PKC2, PKC recognition sequences. Clones for expression in mammalian cells contained an AU1 epitope tag at the carboxyl termini. Dashed lines represent deleted regions; numbers indicate the positions of the amino acids.

View larger version (19K): [in this window] [in a new window]   FIG. 2.   Comparison of amino acid sequence of clone 69 with that of the human TCR. The published sequence of the TCR (61) from the initiating methionine is shown. Dots indicate amino acid identity. Boldface letters indicate different amino acid sequences.

Use of GST fusions to demonstrate specific interaction. To confirm a possible interaction of nef with TCR, nef was expressed as a GST-fusion protein in E. coli, purified, immobilized on glutathione-Sepharose beads, and incubated with lysates of CD4⁺ Jurkat T cells or with lysates of Cos 18 cells which stably express CD8-TCR fusion protein (28). Proteins coprecipitated with nef were separated by electrophoresis in an SDS-12% polyacrylamide gel and electroblotted onto a nylon membrane; the presence of TCR was detected with mouse anti-TCR monoclonal antibody. A protein band of approximately 40 kDa, which was the size of the CD8-TCR fusion protein (22), was specifically detected in the sample containing the extract of Cos 18 cells and GST239nef (Fig. 3). Smaller species of approximately 16 and 22 kDa, which might have resulted from the degradation of the CD8-TCR fusion, were also detected. No CD8-TCR was detected when GST without nef was used (Fig. 3). Similarly, TCR (18 kDa) was also specifically detected when GST-SIVmac239 nef (GST239nef) was incubated with the Jurkat cell lysate (Fig. 3). Again, the nef-TCR interaction was specific, since no TCR was found to interact with GST in the absence of nef. Further evidence for the specificity of the interaction was obtained by the absence of signal when nef of HIV-1 strain SF2 was fused to GST and used for the assays (Fig. 3). The failure of HIV-1 SF2nef to interact with TCR is consistent with other results described in more detail below.

View larger version (45K): [in this window] [in a new window]   FIG. 3.   Binding of GSTnef with TCR in vitro. GST, recombinant GST239nef, and GSTSF2nef proteins were expressed in E. coli, affinity purified, and immobilized by using glutathione-Sepharose beads. The immobilized GST proteins were incubated with extracts of Cos 18 or Jurkat cells previously precleared with immobilized GST and glutathione-Sepharose beads. After extensive washing, proteins coprecipitated with the complexes were analyzed by SDS-12% PAGE, electroblotted onto a nylon membrane, and reacted with anti-TCR monoclonal antibody.

Coprecipitation of TCR and SIVmac239 nef from CD8-TCR-expressing cells. We also analyzed the ability of TCR to be coprecipitated with nef from CD8-TCR-expressing cells. For this purpose, an AU1 epitope tag was placed at the carboxyl terminus of nef-coding sequence in the pFJ expression plasmid and used for transfection into Cos 18 cells, which expressed the CD8-TCR fusion protein. Transfected cell lysates were incubated with anti-AU1 monoclonal antibody, and precipitated proteins were separated by SDS-12% PAGE. The presence of CD8-TCR in the nef immunoprecipitates was detected by reactivity of immunoblotted proteins with anti-TCR monoclonal antibody. Specific coimmunoprecipitation of CD8-TCR was readily detected with nef from SIVmac239 but not from mock-transfected cells (Fig. 4).

View larger version (66K): [in this window] [in a new window]   FIG. 4.   Coprecipitation of TCR with nef from Cos 18 cells. Cos 18 cells expressing CD8-TCR fusion protein were mock transfected (mock) or transfected with plasmids encoding full-length (SIVmac239nef) or truncated (nef1 to nef3-4) SIVmac239 nef. nef immune complexes were precipitated with anti-AU1 monoclonal antibody, washed extensively, resolved by SDS-12% PAGE, and transferred onto a membrane filter. CD8-TCR coimmunoprecipitated with nef was detected by the anti-TCR monoclonal antibody (upper panel). Expression of the full-length or the truncated SIVmac239 nef proteins in the corresponding transfected Cos 18 cells was detected by separating the whole-cell lysates in an SDS-12% polyacrylamide gel, transferring onto a nylon membrane, and probing with the anti-AU1 monoclonal antibody (lower panel).

View larger version (50K): [in this window] [in a new window]   FIG. 5.   SIV nef interacts with the endogenous TCR in Jurkat cells. Jurkat cells were electroporated with expression plasmids encoding SIVmac239 nef, SIVsmH4 nef, or HIV-1 NL4-3 nef (NL4-3) tagged with an AU1 epitope. After 20 h, cells were harvested and nef proteins were immunoprecipitated with anti-AU1 monoclonal antibody. Proteins coimmunoprecipitated with nef were analyzed by SDS-12% PAGE and immunoblotted onto a nylon membrane which was probed with the anti-TCR monoclonal antibody (upper panel). Expression of the nef proteins was detected by separating the whole-cell lysates in an SDS-12% polyacrylamide gel and immunoblotting with the anti-AU1 monoclonal antibody (lower panel).

The central region of SIVmac239 nef is required to bind to TCR. In order to delineate the regions of nef responsible for binding to TCR, a series of SIVmac239 nef deletion mutants was constructed and tested for binding to TCR. All nef mutants contained aa 1 to 15 at the amino terminus for myristoylation. The deletion mutants were constructed with an AU1 tag at the carboxyl terminus and analyzed following transfection of Cos 18 cells. The cells were lysed and incubated with AU1 antibody; precipitated proteins were analyzed by SDS-12% PAGE, and the presence of CD8-TCR was detected by reactivity of anti-TCR monoclonal antibody with immunoblotted proteins (Fig. 4). As shown in the lower panel of Fig. 4, comparable amounts of the full-length and the truncated nef proteins were detected in the transfected Cos 18 cells. When analyzed without heating prior to the electrophoresis, nef5 was detected, and the level of its expression was found to be similar to that of the control (not shown). Deletion of the amino terminus of nef from aa 16 to 97 (nef2 in Fig. 1) did not affect the binding to CD8-TCR (Fig. 4), suggesting that the amino-terminal region of nef which contains the putative SH2 binding motif and the acidic stretch may not be required for interaction with the TCR. However, further deletion up to aa 133, including the putative PXXP motif and the potential PKC phosphorylation site (PKC₁) (nef3 in Fig. 1) resulted in the loss of binding to TCR (Fig. 4). While extensive deletion at the amino-terminal region of nef did not affect its interaction with the TCR, only minor deletion at the carboxyl terminus of the protein was tolerated (nef4 versus nef5 and nef6; Fig. 1 and 4). A minimal construct containing only aa 98 to 235 and 1 to 15 (nef2-4 in Fig. 1) was shown to bind CD8-TCR in these assays (Fig. 4). Thus, we have mapped aa 98 to 235 as a minimal region in nef of SIVmac239 capable of interacting with the TCR.

SIV nef and HIV-2 nef, but not HIV-1 nef, associate with TCR. The failure of TCR to bind to GSTnef from HIV-1 strain SF2 (Fig. 3) and to coimmunoprecipitate with nef from HIV-1 strain NL4-3 in transfected Jurkat cells (Fig. 5) prompted us to investigate further this apparent restriction in specificity. We expressed AU1-tagged nef from five different HIV-1 isolates and analyzed their association with CD8-TCR in transfected Cos 18 cells. NL4-3 and SF2 nef were derived from two laboratory strains of HIV-1, whereas Rulda nef was derived from a primary clinical isolate. nef-153 and nef-259 are derivatives of NL4-3 nef from monkeys with progressive disease following infection by SHIVnef chimeras (16a). Expression plasmids containing these HIV-1 nef genes were transfected into Cos 18 cells, and TCR was detected in immunoprecipitates with TCR monoclonal antiserum as described for the above experiments. In contrast to nef of SIVmac239, which coimmunoprecipitated the TCR, none of the HIV-1 nefs associated with the TCR (Fig. 6, upper panel). Failure to coprecipitate the TCR was not due to instability or inefficient expression of the HIV-1 nef genes, since comparable amounts of the nef proteins were detected in the HIV-1 and the SIV nef-transfected cells (Fig. 6, lower panel).

View larger version (71K): [in this window] [in a new window]   FIG. 6.   HIV-1 nef does not associate with TCR. Cos 18 cells were transfected with expression plasmids encoding SIVmac239 or HIV-1 nef tagged with an AU1 epitope. nef immune complexes were precipitated with anti-AU1 monoclonal antibody. Immunoprecipitation complexes were washed extensively, separated by SDS-12% PAGE, and transferred onto a nylon membrane which was probed with the anti-TCR monoclonal antibody (upper panel). Expression of the nef proteins in the corresponding transfected Cos 18 cells was detected by separating the whole-cell lysates in an SDS-12% polyacrylamide gel, immunoblotting onto a nylon membrane, and probing with the anti-AU1 monoclonal antibody (lower panel). Lanes: 1, mock transfected; 2, wild-type SIVmac239 nef; 3 and 4, HIV nef obtained from two animals (259 and 153, respectively) infected with a recombinant SIV in which the SIV nef gene was replaced by the HIV NL4-3 nef allele (SHIVnef); 5, HIV nef derived from a primary clinical isolate (Rulda); 6, HIV-1 SF2 nef; and 7, HIV-1 NL4-3 nef.

View larger version (51K): [in this window] [in a new window]   FIG. 7.   SIV and HIV-2 nef, but not HIV-1 nef, associates with TCR in yeast cells. S. cerevisiae Y190 was cotransformed with hybrid plasmid encoding the fusion protein of the GAL4 DNA binding domain and the HIV-1 consensus nef (pBD-consnef), HIV-1 NL4-3 nef (pBD-NL4-3), SIVsmH4 nef (pBD-SIVsmH4), SIVmac239 nef (pBD-SIVmac239), or HIV-2 ST nef (pBD-HIV-2ST) alone (left panel) or in combination with the pAD-cDNA69 (right panel). Transformed yeast cells were plated onto LYH+3AT plates, and colonies were assayed for the production of -galactosidase (blue). The top panel indicates the positive and negative controls with the yeast cells transformed with expression plasmids of pVA3-1 (p53) and pTD1-1 (SV40 T-Ag), vectors with no inserts (pBD+pAD), or pAD-cDNA69 (TCR) alone.

Tyrosine kinase activity in nef complexes. Protein tyrosine phosphorylation is one of the earliest biochemical events elicited by stimulation of B-cell receptor (BCR) and TCR in B and T cells, respectively (10, 29). Neither the BCR nor the TCR has intrinsic tyrosine kinase activity; both appear to interact with cytoplasmic protein tyrosine kinase (PTK). Three cytoplasmic PTKs (lck, fyn, and ZAP70) have been implicated in intracellular TCR signal transduction (11, 12, 48, 58). In the ensuing experiment, we asked if the nef-TCR complex coprecipitated with a PTK. Immobilized GSTnef from SIVmac239 was incubated with the Jurkat cell lysate as described previously. After extensive washing, the coprecipitation complexes were incubated under the conditions for assay of kinase activity in the presence or absence of ATP. The reaction products were analyzed by SDS-12% PAGE and immunoblotted onto a nylon membrane. Tyrosine-phosphorylated proteins were detected with an antiphosphotyrosine antibody. In the sample supplied with ATP, two major bands with migration corresponding to the molecular weights of GSTnef and TCR were detected by phosphotyrosine-specific antiserum (Fig. 8, upper panel, lane 2). The presence of TCR was confirmed when the same blot was reprobed with TCR-specific antiserum (Fig. 8, lower panel, lane 2). In contrast, tyrosine phosphorylation of the protein band with a molecular weight similar to that of GSTnef was not detected in the sample without the addition of ATP (Fig. 8, upper panel, lane 3). No tyrosine phosphorylation was observed in the samples without prior incubation with the Jurkat cell lysate (Fig. 8, lanes 7 and 8).

View larger version (67K): [in this window] [in a new window]   FIG. 8.   TCR-nef complex contains tyrosine kinase activity. Affinity-purified and immobilized recombinant GST or GSTnef was incubated in the presence of Jurkat T or J.CAM1.6 cell lysates previously precleared with immobilized GST and glutathione-Sepharose beads. Complexes precipitated with GSTnef were washed extensively and incubated in kinase buffer in the presence or absence of ATP. The reaction products were analyzed by SDS-12% PAGE, transferred onto a nylon membrane, and probed with antiphosphotyrosine monoclonal antibody. As negative controls, GST and recombinant GSTnef were purified from the bacterial lysates and assayed directly for kinase activity without prior incubation with the cell extracts (upper panel). To confirm the presence of TCR in the coprecipitation complexes, antibodies were stripped and the filter was reprobed with the anti-TCR monoclonal antibody (lower panel).

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

The TCR is a multimolecular complex containing the polymorphic TCR  and  subunits, the invariant CD3 , , and  chains, and a homodimer of  chains or, in a minority of receptors, a heterodimer of  and  chains (4, 13, 20). The disulfide-linked TCR  and  heterodimer is responsible for antigen recognition, but the short, 5-aa cytoplasmic domains of the TCR  and  subunits are insufficient to couple to the intracellular signaling molecules. In contrast, the  chain has an extracellular region of only 9 aa but an extensive intracellular domain of 113 aa (60). Using a chimeric protein consisting of the extracellular and transmembrane domains of CD8 fused to the cytoplasmic domain of the  chain, Irving and Weiss elegantly showed that this fusion protein could elicit transducing signals indistinguishable from those generated by the intact TCR (28). One characteristic feature of the  chain is the presence of the immunoreceptor tyrosine-based activation motif (ITAM) (EX₂YX₂L/IX₇YX₂L/I), which is crucial for  chain coupling to the intracellular tyrosine kinases and adapter proteins and, hence, is absolutely required for all subsequent TCR signaling responses (8, 12, 41). Phosphorylated ITAM sequences function as SH2 binding domains (27, 44). One of the earliest biochemical events in TCR signaling is the activation of the src family tyrosine kinase lck, which in turn recruits various enzymes and signaling molecules leading to an altered pattern of gene expression and cellular activation (9-11, 40).

We used a truncated SIVmac239 nef as bait to screen an activated lymphocyte cDNA library in a yeast two-hybrid system. We identified TCR as one of the cellular proteins associating with nef. The clone that was identified in the screen actually differed from the published sequence of TCR (61) at three amino acid positions. The reasons for this are not clear. However, specific binding of nef to authentic TCR present in Jurkat cells and to authentic TCR sequences present in the CD8-TCR fusion in Cos 18 cells was demonstrated. Binding of TCR to full-length nef was demonstrated in vitro (Fig. 3) and in cells coexpressing TCR and nef (Fig. 4, 5, and 6). The association with TCR was specific for SIVmac, SIVsm, and HIV-2 nef but was not observed with HIV-1 nef. In addition, the nef-TCR complex coprecipitated active tyrosine kinase, which is present in Jurkat cells but not in J.CAM1.6 cells lacking a functional Lck. Finally, using a series of amino- and carboxyl-terminal deletion mutants of SIVmac239 nef, we mapped the central core region (aa 98 to 235) of nef responsible for binding to the  chain.

We can speculate that the binding of SIV nef to TCR might be related to reported activities of nef in causing T-lymphocyte activation. An unusual nef allele of SIV, called Y nef, is responsible for causing activation of primary lymphocytes in culture and an unusually acute disease course in monkeys (17, 18). Natural nef alleles of both SIV and HIV allow virus replication in an IL-2-dependent cell line and activate the production of IL-2 from these cells (3). HIV-1 nef was earlier reported to cause activation signals in Jurkat cells (6). HIV-1 nef has a highly conserved SH3 binding element, PXXPXXP, which is principally but not exclusively responsible for binding to src family kinases (34). SIV and HIV-2 nefs have a single PXXP element, and, although these can bind src family kinases, they appear to do so less well than HIV-1 nefs (14, 18, 46). Perhaps HIV-1 nef can influence signaling by direct interaction with src family kinases through the highly conserved PXXPXXP SH3 binding domain, while nef of SIVmac, SIVsm, and HIV-2, as an alternative means to the same end, may interact first with TCR. The interaction of nef with a TCR-kinase complex could result in tyrosine phosphorylation of nef, perhaps on a conserved YXXL sequence near the amino terminus which resembles an SH2 binding domain, and this could in turn result in recruitment of other tyrosine kinases through the phosphorylated SH2 binding domain. Thus, the picture that emerges from this scenario is that both HIV-1 nef and SIVmac, SIVsm, and HIV-2 nef may affect signaling through tyrosine kinases but that they may rely on a slightly different combination of cellular partners and binding domains to achieve these ends.