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Journal of Virology, November 1998, p. 9337-9344, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Coreceptors Other Than CCR5 by Non-Syncytium-Inducing Adult and Pediatric Isolates of Human Immunodeficiency Virus Type 1 Is Rare In Vitro

Yi-jun Zhang,1 Tatjana Dragic,1 Yunzhen Cao,1 Leondios Kostrikis,1 Douglas S. Kwon,2 Dan R. Littman,2,3 Vineet N. KewalRamani,2 and John P. Moore1,*

Aaron Diamond AIDS Research Center, The Rockefeller University,1 and The Skirball Institute of BioMolecular Medicine,2 and Howard Hughes Medical Institute,3 New York University School of Medicine, New York, New York 10016

Received 1 June 1998/Accepted 4 August 1998

    ABSTRACT
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We have tested a panel of pediatric and adult human immunodeficiency virus type 1 (HIV-1) primary isolates for the ability to employ the following proteins as coreceptors during viral entry: CCR1, CCR2b, CCR3, CCR4, CCR5, CCR8, CXCR4, Bonzo, BOB, GPR1, V28, US28, and APJ. Most non-syncytium-inducing isolates could utilize only CCR5. All syncytium-inducing viruses used CXCR4, some also employed V28, and one (DH123) used CCR8 and APJ as well. A longitudinal series of HIV-1 subtype B isolates from an infected infant and its mother utilized Bonzo efficiently, as well as CCR5. The maternal isolates, which were syncytium inducing, also used CXCR4, CCR8, V28, and APJ.

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Human immunodeficiency virus type 1 (HIV-1) enters CD4+ cells by fusion at the plasma membrane after interactions with CD4 and coreceptor molecules (6, 8, 23, 53). The first coreceptor to be identified was the CXC chemokine receptor CXCR4 (30), closely followed by the CC chemokine receptor CCR5 (3, 17, 21, 24, 25). These two proteins are generally considered to be the most important coreceptors used by HIV-1 strains of the T-tropic and M-tropic phenotypes, respectively (6, 8, 23, 53) (see reference 7 for a discussion of how HIV-1 phenotype is related to coreceptor usage). There is strong epidemiological support for this view in the case of CCR5 (19, 38, 46, 63, 80).

At least nine other chemokine receptors, or structurally related molecules, have also been described as supporting HIV-1 env-mediated membrane fusion or viral entry in vitro. These include CCR2b (1, 24, 31), CCR3 (2, 5, 17, 24, 34, 59-61), BOB/GPR15 (20, 29), Bonzo/STRL33/TYMSTR (20, 45, 47), GPR1 (29), CCR8 (37), US28 (57), V28/CX3CR1 (58, 61), and APJ (16, 27). There is good evidence that CCR3 can be used efficiently by a significant fraction of HIV-1 isolates in vitro, provided the expression of this protein in transfected cells is boosted (2, 5, 59, 61).

Several studies have compared coreceptor usage by primary HIV-1 isolates of several genetic subtypes, but the emphasis has usually been on studying CCR1 to CCR5 and CXCR4 (9, 18, 65, 69, 76, 79). In general, the results were similar to those from more limited studies of the individual receptors; other than the occasional use of CCR3, only CCR5 and CXCR4 were efficiently used by the majority of the isolates tested (9, 18, 65, 69, 76, 79). It is therefore not clear how general, and how efficient, the use by HIV-1 of the more recently identified potential coreceptors actually is. Knowing the range of coreceptors that could be used by HIV-1 in vivo is important for the development of antiviral drugs aimed at inhibiting HIV-1 entry. How many different potential coreceptors must be blocked? To address this question, we have compared the usage of CCR1, CCR2b, CCR3, CCR4, CCR5, CCR8, CXCR4, Bonzo, BOB, GPR1, V28, US28, and APJ by collections of primary HIV-1 isolates of both adult and pediatric origins. We tested pediatric isolates because the unusual characteristics of HIV-1 infection in infants could, in principle, be influenced by a cell tropism pattern that is in turn, determined by the use of coreceptors other than CCR5 or CXCR4 (10, 70, 77).

env complementation assay of coreceptor use. We first used an env complementation assay to measure the entry of a luciferase-containing HIV-1 reporter virus into U87-CD4 cells transiently transfected with a pcDNA3.1 plasmid encoding either CCR1, CCR2b, CCR3, CCR5, CCR8, CXCR4, Bonzo, BOB, or GPR1 (Table 1). U87-CD4 cells transfected with the unmodified pcDNA3.1 plasmid were used as a control. Entry mediated by seven different HIV-1 envelope glycoproteins was assessed 2 days later by the measurement of luciferase activity in triplicate, essentially as described previously (18, 21, 25, 26). The sources of the env genes have been recorded elsewhere (25, 26), except for Gun1 and Gun1V, which were cloned from isolates provided by Paul Clapham and Hiroo Hoshino (49, 72).

                              
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  		TABLE 1.   env complementation assay of HIV-1 entry into U87-CD4 cells transfected with different coreceptors

Only CCR5 and/or CXCR4 were used efficiently by five of the seven test viruses (boldface values in Table 1). Gun1 and, to a greater extent, Gun1V were able to enter via GPR1 as well as by CXCR4. Note that Gun1V weakly entered U87-CD4 cells transfected with the control plasmid or with several coreceptors, as reported previously (49). This probably reflects limited usage by Gun1V of a coreceptor endogenous to U87-CD4 cells, probably GPR1 (29).

Coreceptor use in stably transfected GHOST cells. Human osteosarcoma HOS-CD4 cells stably transduced with different potential coreceptors and the green fluorescent protein (GFP) reporter gene under the control of the HIV-2 long terminal repeat (designated GHOST cells) were used to assess the replication of a much larger panel of pediatric and adult primary isolates and clones (Table 2). These cells have been described elsewhere (41, 74). In total, 14 pediatric isolates and 13 viruses of adult origin were tested against 13 different cell lines (GHOST cells expressing CCR1, CCR2b, CCR3, CCR4, CCR5, CCR8, CXCR4, Bonzo, BOB, GPR1, V28, US28, or APJ). The pediatric isolates were obtained from the Pediatric AIDS Foundation's Ariel Project (11); the sources of most of the adult viruses have been described elsewhere (74, 75). A viral inoculum of 10 ng of p24 per well (24-well plate) was used, with the endpoint being HIV-1 replication, assayed by p24 antigen production on day 6 postinfection.

                              
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  		TABLE 2.   Coreceptor usage of pediatric and adult primary HIV-1 isolates in GHOST-CD4 cells expressing different coreceptors

Again, with rare exceptions, only CCR5 and CXCR4 were used efficiently by the HIV-1 isolates. Classified according to the ability to replicate on MT-2 cells (73), with one exception, the non-syncytium-inducing (NSI) viruses all used CCR5 only. In contrast, the syncytium-inducing (SI) isolates had a broader pattern of coreceptor recognition (Table 2). All four SI viruses used CXCR4, and three used CCR5 as well. Two of the SI viruses (301593 and AD-73) also utilized V28 weakly, and the DH123 clone (66) used CCR8, V28, and APJ efficiently, in addition to CCR5 and CXCR4. A simian immunodeficiency virus isolate (SIVlib) was also tested (13); it replicated in cells expressing CCR5, Bonzo, BOB, and (to a lesser extent) GPR1, consistent with earlier studies (4, 13, 20, 27, 29, 48, 61).

Overall, most of the GHOST cell lines supported the entry of at least one HIV-1 or SIV strain, confirming the expression of the transfected coreceptor at a functional level. In addition, the CCR1-, CCR2b-, CCR3-, CCR4-, CCR5-, CXCR4-, Bonzo-, and BOB-expressing GHOST cell lines have each been shown to support the entry of at least three different HIV-2 primary isolates, confirming that the coreceptors are properly expressed on these cells (54). No such positive control exists for the GHOST-US28 cells. In the absence of monoclonal antibodies specific for these and other potential coreceptors, we cannot compare the levels to which they are expressed on the surfaces of GHOST cells and relevant primary cells.

A pediatric HIV-1 isolate uses Bonzo efficiently. A single pediatric isolate (P6 02-217-V3) replicated efficiently in GHOST cells expressing CCR5 or Bonzo (Table 2). Since this was the only virus in the test panel to utilize Bonzo, we tested six longitudinally obtained isolates from the same male infant (P6) and three from its mother (M6). The mother was resident in Florida when she became HIV-1 infected through heterosexual intercourse, but she has since died of AIDS. Infant P6 was M6's second child, an older boy having been also vertically infected with HIV-1 (no samples were available from either the elder child or his father at the time this study was performed).

The infant isolates (P6 series; m indicates the month postbirth) were all of the NSI phenotype, and the maternal isolates (M6 series) were all SI (Table 3). The first five infant isolates and all three from the mother used both CCR5 and Bonzo, and the last isolate from the infant used only CCR5. In addition to CCR5, Bonzo, and CXCR4, the maternal isolates utilized CCR8, V28, and APJ (Table 3). With the exception of the last isolate from the infant (P6-m36), the replication of the infant and maternal isolates in Bonzo-expressing GHOST cells was robust, being comparable to the replication of the same isolates in the CCR5-expressing cells (Fig. 1a and b and data not shown). The last-obtained infant isolate (P6-m36) did not, however, replicate in Bonzo-expressing GHOST cells at any inoculum size tested, although its replication in CCR5-expressing cells was strong (Fig. 1c). Presumably, and for reasons unknown, phenotypic evolution in the infected infant has led to the loss of Bonzo usage but the retention of CCR5 usage.

                              
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  		TABLE 3.   Coreceptor usage of sequential primary HIV-1 isolates from a mother-child transmission paira


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  		FIG. 1.   Use of CCR5 and Bonzo by HIV-1 isolates from a mother and her child. The replication of two isolates (m1 and m18) from infant P6 and of two isolates (m1b and m4b) from his mother (M6) in GHOST cells expressing CCR5 (a) or Bonzo (b) is indicated by the amount of p24 antigen production at 3 days (shaded bars) and 6 days (black bars) postinfection. Infant isolates P4 and P9 are also shown for comparison (see Tables 2 and 3). (c) Replication of different inocula of the last-obtained isolate (m36) from infant P6 in GHOST cells expressing CCR5 (shaded bars) or Bonzo (black bars), as determined by p24 antigen production 4 days postinfection.

Most HIV-1 isolates do not use Bonzo efficiently (Tables 1 and 2). However, coreceptor usage by HIV-2 is broad in comparison to that by HIV-1 (10, 20, 28, 36, 50, 54, 58, 71), and some HIV-2 strains use Bonzo quite well (20, 54). We therefore considered it possible that the P6 and M6 isolates might contain HIV-2 in addition to HIV-1, perhaps as a result of dual infection of the mother. Although culture supernatants from these isolates were weakly positive in commercial HIV-2 antigen assays, so were some known HIV-1 isolates (data not shown). Antigenic similarities between the HIV-1 and HIV-2 core antigens reduce the significance of such findings. Serology studies (Western blots using commercial reagents) on the mother and infant revealed no evidence of HIV-2 infection (data not shown). Furthermore, no HIV-2 sequences could be amplified from peripheral blood mononuclear cells (PBMC) infected with P6 or M6 isolates by using two different sets of nested PCR primer pairs that have each successfully amplified HIV-2 gag and env fragments from primary PBMC DNA in other studies (14, 32).

In contrast, when nested PCR primers designed to amplify the C2-to-C4 env region of all genetic subtypes of the HIV-1 M group were used (43, 44), nucleotide sequences associated with HIV-1 subtype B were obtained. The maternal and infant isolates clustered tightly together in a phylogenetic analysis (100 of 100 bootstrap replicates, using the neighbor-joining program of PHYLIP), consistent with the known vertical transmission. BLAST was used to demonstrate that the sequences obtained from the mother and infant were not a contaminant of pre-existing strains (no sequence was more than 95% similar to anything found in GenBank) (68). The recombination identification program RIP was used to show that, over the region tested, there was no evidence of recombination between the B subtype and any other subtype---the sequence was clearly subtype B throughout (68). More detailed analyses of the genetic sequences and the coreceptor usage properties of clones derived from the HIV-1 isolates from this maternal-infant transmission case are in progress.

Conclusions. We have found that efficient use of coreceptors other than CCR5 and CXCR4 by HIV-1 is rare in vitro. We observed expanded coreceptor recognition only by SI viruses that can use both CCR5 and CXCR4, including SI viruses from an infected mother who transmitted NSI viruses to her child (Tables 2 and 3). The expanded tropism of SI viruses has been documented previously (9, 16, 18, 27, 65, 69, 76, 79).

One aspect of our conclusions should be tempered. The expression of CCR3 is known to be unusually inefficient in transfected cells, in comparison to other seven-transmembrane-spanning receptors (2, 5, 17, 61). In principle, this could mean that some viruses able to use CCR3 in vivo might not be able to enter CCR3-transfected cells in vitro, leading to false-negative conclusions about CCR3 usage. However, we used CCR3-specific murine monoclonal antibody 7B11 (35) to determine the extent of CCR3 expression on both GHOST-CCR3 cells and mitogen-activated PBMC. CCR3 was detected by this antibody on 25% of the GHOST-CCR3 cells but on only about 1% of activated PBMC (data not shown). The rarity of CCR3-expressing cells among PBMC has been reported previously (62). The fluorescence intensity of CCR3 staining on the GHOST cells was comparable to, or greater than, the intensity on the rare CCR3-positive cells in PBMC (data not shown). Thus, it is unlikely that the GHOST-CCR3 cells would fail to detect any CCR3 usage by viruses that entered activated PBMC via this coreceptor. The possibility remains that CCR3 is an important coreceptor on a minor subset of CD4+ T cells in vivo (62).

None of the isolates we tested used CCR1, CCR2b, CCR4, BOB (GPR15), or US28 for entry into transfected GHOST cells. The failure of any HIV-1 isolate to use CCR4 is notable in the context of a report that macrophage-derived chemokine, a CCR4 ligand (39), inhibits the replication of several NSI and SI HIV-1 isolates in vitro (55). The only isolates able to use Bonzo (also known as STRL33 or TYMSTR) were genetically related SI and NSI strains from a maternal-infant vertical transmission case. The HIV-1 SI isolates all used CXCR4, as expected from multiple previous studies (9, 18, 65, 69, 76, 79). However, the SI isolates also had an expanded tropism for other coreceptors. The M6 series of maternal SI isolates, along with three other SI viruses, used V28 (CX3CR1), albeit to various extents. Only the M6 isolates and the DH123 molecular clone entered CCR8- or APJ-expressing GHOST cells efficiently (Tables 2 and 3), although we were unable to demonstrate CCR8 use by DH123 in transiently transfected U87-CD4 cells (Table 1). This difference may reflect the larger infectious inoculum used with the GHOST cells. Only Gun1V and, to a lesser extent, the related Gun1 virus entered GHOST cells via GPR1. In general, the usage of GPR1, CCR8, V28, and APJ by a subset of SI viruses is in accord with earlier reports on these coreceptors (16, 27, 29, 37, 58, 61).

Positive use of Bonzo, BOB, and GPR1 was, however, shown by SIVlib, which confirms the functional expression of these coreceptors on the GHOST cell lines. Bonzo, BOB, and GPR1 are known to be highly efficient coreceptors for a range of strains from the SIV grouping (4, 20, 29), as is CCR5 (13, 15, 20, 27, 48, 61). Likewise, several HIV-2 primary isolates can enter CCR1-, CCR2b-, and CCR4-expressing GHOST cells, confirming that they also express the transfected coreceptor appropriately (54).

What does a negative result mean in in vitro assays of HIV-1 replication in transfected cells? Previous reports have described efficient use by HIV-1 of several of the "newer" coreceptors (16, 20, 27, 29, 37, 45, 47, 57, 58). Often, but not always, the viruses able to use these coreceptors were "T-tropic" or SI strains. We can confirm that SI viruses can indeed use multiple coreceptors in vitro (Tables 1 and 2). When comparing different experimental systems, a relevant issue is, we believe, one of efficiency of coreceptor usage. In several earlier reports, the assays most commonly employed were those of env-mediated membrane fusion, sometimes in association with measurements of the entry of env-complemented, reporter gene-expressing HIV-1 pseudotypes. These assays can be quite sensitive. However, we have noted circumstances in which a positive result in a membrane fusion assay is not mirrored in a virus entry assay using the same envelope glycoproteins and coreceptors (26). This is probably because virus entry has a more stringent requirement for high-affinity virus-receptor interactions, because of the lower number of envelope glycoprotein complexes on virions than on the surfaces of fusing cells. Overexpression of lower-affinity coreceptors in cell-cell fusion systems can compensate for the reduced efficiency of individual coreceptors, but this is not necessarily predictive of cell-free virus entry (26).

In an env complementation test of virus entry, what is a significant result? The luciferase expression assay can have an extremely wide dynamic range (Table 1). A level of virus entry that is 10-fold over the background in the presence of a particular coreceptor is clearly a demonstration that the virus is capable of interacting with that coreceptor. But if the level of entry is still 100- to 1,000-fold lower than that achieved with CXCR4 or CCR5, as is often the case, that needs to be taken into account when judging whether the interaction is likely to be biologically significant. For example, as shown in Table 1, HIV-1 NDK enters U87-CD4-GPR1 cells 7-fold more efficiently than it enters parental U87-CD4 cells, but entry via GPR1 is 493-fold less efficient than via CXCR4. We believe that the latter value is the more relevant of the two. In the present study, we have mostly used virus replication as an endpoint (Tables 2 and 3). Our use of a large inoculum in these assays reduces the possibility of a false-negative result. In terms of viral output, we are unlikely to detect a weak level of replication that is 100-fold less than what we score as positive (+) (Table 2). However, HIV-1 entry and replication at such a low level are not likely to be important.

The existence of "natural knockouts" for CCR5 (19, 37, 45, 61, 78), combined with the multiplicity of studies that demonstrate ligand-sensitive, efficient use of this coreceptor in vitro (3, 9, 17, 18, 21, 24, 25, 40, 65, 69, 75, 76, 79), has led to the firm conclusion that CCR5 is of paramount importance for HIV-1 transmission and replication in vivo (6, 8, 23, 53). The acquisition of efficient, ligand-sensitive CXCR4 use in vitro by HIV-1 variants isolated at a time when there are alterations in CD4 cell loss and a more rapid disease course in vivo (9, 18, 65, 69) speaks to the physiological importance of this coreceptor. But are other coreceptors important in vivo? Tissue expression patterns are a relevant factor to consider. To be involved in the maintenance of high viral loads, a coreceptor would have to be expressed on activated CD4+ T cells (56). Whether this occurs is far from certain for many of the coreceptors described to date. A significant problem is the unavailability of specific chemokine ligands and/or monoclonal antibodies for many coreceptors. This makes it difficult to judge the importance of individual coreceptors in cells other than transfectants. Blocking HIV-1 entry with the CCR3 ligand eotaxin was a major contribution to demonstrating that CCR3 was a coreceptor in microglia (34), although there is now no consistent agreement as to the relative importance of CCR3 and CCR5 in these cells (33, 67). HIV-1 inhibition studies have been performed with the I-309 ligand for CCR8, but only in CCR8-transfected cells (37). However, wherever it is expressed in vitro (and perhaps also in vivo), the presence of a potential coreceptor on a cell (CD4+ or CD4-) does not prove that it can be used efficiently in that cellular context (22).

Many SI isolates can clearly use multiple coreceptors in vitro. Are these coreceptors used by SI viruses in vivo? Of note is the fact that an SI isolate from an individual homozygous for defective CCR5 alleles was shown to use only CXCR4 when it was tested for the ability to enter GHOST cells expressing CCR1, CCR2b, CCR3, CCR4, CCR5, CXCR4, BOB, Bonzo, or GPR1 (51). Thus, inability to use CCR5 in vivo does not necessarily drive HIV-1 to use a coreceptor other than CXCR4. In addition, any alterations in envelope glycoprotein structure that might be necessary for a HIV-1 strain to switch from using CCR5 or CXCR4 to using a coreceptor such as BOB or Bonzo, whether under drug selection pressure or spontaneously, would have to be compatible with the retention of resistance to humoral immunity. The latter is also a property of the envelope glycoproteins, and a neutralization-sensitive variant may well not persist in the host (52, 74).

Overall, we believe that there is no compelling evidence for the importance of coreceptors other than CCR5 and CXCR4 when considering HIV-1 infection of blood cells of the lymphoid and monocyte lineages in vivo. A proviso is that the expression and use of different coreceptors in other cell types, for example, in the brain or thymus, could contribute to some facets of HIV-1 pathogenesis in adults or in children (6, 8, 23, 53).

We observed that a mother (M6) who was infected with SI viruses able to use CXCR4 as well as CCR5 transmitted only NSI, CCR5-using viruses to her infant (Tables 2 and 3). This pattern of phenotypic selection has been previously documented in maternal-infant transmission of HIV-1 (11, 64), although the converse pattern was noted in a single case of HIV-2 vertical transmission (12). As noted above, both the maternal and infant isolates also used Bonzo, and the maternal SI isolates used several coreceptors. However, with this exception, we noted nothing unusual about the coreceptor usage patterns of pediatric HIV-1 isolates that could distinguish them from adult isolates. Overall, the characteristics of the developing immune system probably have more influence on the nature of pediatric HIV-1 infection than do any unusual properties of the infecting HIV-1 strains (42, 78).

    ACKNOWLEDGMENTS

We are very grateful to Alexandra Trkola for helpful discussions on virology, to Bette Korber for serious advice on genetics, and to Beatrice Hahn for consultations. We appreciate the contributions of the donors of HIV-1 isolates and clones used in this study, including the participants in the Pediatric AIDS Foundation's ARIEL Project. We particularly thank Zhi-wei Chen and Preston Marx for gifts of SIVlib and HIV-2 primers, Hiroo Hoshino and Paul Clapham for the Gun1 and Gun1V isolates, WenKai Xiang for provision of cell lines, Paul McHardy and Hugh Jarce for technical support, and Ana Puga for clinical information on the HIV-1 vertical transmission case.

This study was supported by NIH grant AI41420 and by the Pediatric AIDS Foundation. V.N.K. is supported by a postdoctoral fellowship from the Damon Runyon/Walter Winchell Foundation, D.R.L. is an Investigator of the Howard Hughes Medical Institute, and J.P.M. is an Elizabeth Glaser Scientist of the Pediatric AIDS Foundation.

    FOOTNOTES

* Corresponding author. Mailing address: Aaron Diamond AIDS Research Center, 455 1st Ave., 7th floor, New York, NY 10016. Phone: (212) 725-0018. Fax: (212) 725-1126. E-mail: jmoore{at}adarc.org.

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Journal of Virology, November 1998, p. 9337-9344, Vol. 72, No. 11
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