JVI Accepts, published online ahead of print on 1 April 2009

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J. Virol. doi:10.1128/JVI.00384-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Multiply-deleted replication-defective adenovirus vectors do not induce measurable vector-specific T cells in human trials

Richard A. Koup^*, Laurie Lamoreaux, David Zarkowsky, Robert T. Bailer, C. Richter King, Jason G. D. Gall, Douglas E. Brough, Barney S. Graham, and Mario Roederer

Immunology Laboratory, Immunology Core Section, Clinical Trials Core, and ImmunoTechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA; GenVec Inc. Gaithersburg, MD, USA

^* To whom correspondence should be addressed. Email: rkoup{at}mail.nih.gov.

The magnitude and character of adenovirus type 5 (Ad5) -specific T cells was determined in volunteers with and without pre-existing neutralizing antibodies (NA) to Ad5 who received rAd5-based HIV vaccines. There was no correlation between T cell responses and NA to Ad5. There was no increase in magnitude or activation state of Ad5-specific CD4⁺ T cells at time points where antibodies to Ad5 and T cell responses to the recombinant gene products could be measured. These data indicate that rAd5-based vaccines containing deletions in the E1, E3 and E4 region do not induce appreciable expansion of vector-specific CD4⁺ T cells.

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